• 제목/요약/키워드: extraction condition

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Developement of Quantitative Extraction Method of Amygdalin without Enzymatic Hydrolysis from Kyonin(Armeniacae Semen) by High Performance liquid Chromatography

  • Kim, Dong-Min;Hong, Seon-Pyo
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.388.3-389
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    • 2002
  • Kyonin(Armeniacae Semen)is the herb medicine that contains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde. HCN. and glucose by emulsin. a hydrolysis enzyme in kyonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula kyonin was 0.5% from crude powers. 0.7% from small pieces. 1.2% from half pieces and 2.7% from whole pieces. (omitted)

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Scenedesmus sp.로부터 Taguchi 법을 이용한 지방추출의 최적화 (Optimization of Lipid Extraction from Scenedesmus sp. Using Taguchi Approach)

  • 김나영;오성호;최운용;이현용;이신영
    • KSBB Journal
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    • 제25권4호
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    • pp.371-378
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    • 2010
  • For the biodiesel fuel production from microalgae, the lipid from wet and dry samples of green algae Scenedesmus sp. was extracted by using various solvents and pre-treatment methods. Extraction yield of the lyophilized sample was better than that of dry sample. Chloroform/methanol (2:1, v/v) and ultrasonication or homogenization method were also selected as the most effective solvent and pre-treatment methods for lipid extraction, respectively. Under these constraint conditions, optimization experiment of lipid extraction was investigated by Taguchi approach using orthogonal matrix $L_9$ ($3^4$) method. The optimum extraction conditions of lipid extraction was obtained at pre-treatment of homogenization, extraction time of 5 hour, temperature of $35^{\circ}C$, and solvent ratio of 1:20 (w/v). Yield of extraction at optimized condition was 20.55% and it was 96% of total lipid content (21.38%) of Scenedesmus sp.

반응 표면 분석에 의한 캐모마일(Anthemis nobilis) 첨가 홍차의 최적 추출조건 (Optimization of Extraction Condition for Black Tea with Chamomile (Anthemis nobilis) by Response Surface Methodology)

  • 임오준;변광인;이병구
    • 한국식품조리과학회지
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    • 제23권6호
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    • pp.954-960
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    • 2007
  • The study was designed to determine optimum extraction conditions, by the factorial design of 3 variables and 3 levels using an a RSM program. To determine the optimal conditions for the extraction of black tea with chamomile, the experimental design was applied. The independent factors were the extraction temperature ($60^{\circ}C$, $80^{\circ}C$, $100^{\circ}C$), extraction time (2 min, 4 min, 6 min), and chamomile addition (0.3%, 1.0%, 1.7%). By the RSM (response surface methodology) analysis of the black tea with chamomile extraction, we found that the extraction temperature greatly affected the extraction yield, brix, pH, and reducing sugar, caffeine, theobromine and theophillin contents. The optimum extraction time, temperature, and chamomile content were 4 min, $90^{\circ}C$, and $1.6{\sim}1.7%$, respectively.

Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage

  • Shin, S.C.;You, S.J.;An, B.K.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권4호
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    • pp.601-604
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    • 2006
  • The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharide-protein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

스트립 배열된 다수 부이에 의한 파력에너지 추출 (Wave Power Extraction by Strip Array of Multiple Buoys)

  • 조일형
    • 한국해양공학회지
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    • 제28권5호
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    • pp.474-483
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    • 2014
  • The majority of existing WECs (wave energy converters) are designed to achieve maximum power at a resonance condition. In the case of a single WEC, its size must be large enough for tuning, and it has high efficiency only within a limited frequency band. Recently, wave power extraction by deploying many small buoys in a compact array has been studied under the assumption that the buoy's size and separation distance are much smaller than the water depth, wave length, and size of the array. A boundary value problem involving the macro-scale boundary condition on the mean surface covered by an infinite strip of buoys is solved using the eigenfunction expansion method. The energy extraction efficiency (${\varepsilon}=1-R^2_f-T^2_r$), where $R_f$ and $T_r$ are the reflection and transmission coefficients for a strip array of buoys, is assessed for various combinations of packing ratio, strip width, and PTO damping coefficient.

정상 환경 상태에서 다중 빔 탐색 레이다의 표적 고도 추출 (Target Altitude Extraction for Multibeam Surveillance Radar in Normal Environmental Condition)

  • 정명수;박동철
    • 한국전자파학회논문지
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    • 제18권9호
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    • pp.1090-1097
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    • 2007
  • 다중 빔 탐색 레이다는 디지털 빔 형성 기술을 이용하여 수신시 적층 빔을 형성하는 최신의 3D(3 Dimensional) 레이다 기술이다. 본 논문에서는 다중 빔 3D 레이다의 빔 형성기 설계 개념 및 다중 경로 현상이 없는 정상 환경 상태에서 표적 고도 추출 방법을 제안하고 고찰해 보고자 한다. FFT(Fast Fourier Transform)수신 빔형성기에서 안테나 정현 공간(sine space) 좌표계 기반으로 표적 고도 추출 알고리듬에 대해 기술하였다. 제안된 알고리듬은 1개의 look-up 테이블을 이용하여 다양한 고도 및 레이다 주파수 대역에 대해서 일치하는 결과가 나오는 것을 시뮬레이션으로 확인하였다.

초임계유체 추출에 의한 생강(Zingiber officinale Roscoe) Oleoresin의 항균활성 (Antimicrobial Activity of Ginger(Zingiber officinale Roscoe) Oleoresin by Supercritical Fluid Extraction)

  • 이명희;이경혜;김경탁;김성수
    • 한국식품위생안전성학회지
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    • 제27권2호
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    • pp.109-116
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    • 2012
  • 본 연구에서는 에탄올추출과 초임계추출(SFE) 조건에 따른 생강 oleresin(GO) 추출물의 Gram 양성균(Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes)과 Gram 음성균(Pseudomonas aeruginosa, Escherichia coli)에 대한 항균활성을 살펴보았다. GO 에탄올추출은 추출물의 농도가 증가할수록 clear zone이 비례적으로 증가하였다. 이의 항균성은 Gram 양성균이 Gram 음성균보다 크게 나타났으며, 특히 Listeria monocytogenes에서 항균활성이 크게 나타났다. 생강분말과 에탄올의 추출비율이 1:6인 E-III 처리구가 항균활성이 높았다. GO 에탄올추출물의 항균성은$80^{\circ}C$ 조건에서 처리하는 것이 효과적이었고, 추출시간 (1시간~7시간)은 처리구 간에 큰 차이가 없었다. 초임계추출물에 대한 항균성은 Listeria monocytogenes에서 가장 크게 나타났다. 초임계추출물에서는 100 bar $35^{\circ}C$, 250 bar $35^{\circ}C$, 250 bar $65^{\circ}C$ 조건에서 강한 항균력을 보였다. 그리고 용매추출물의 경우 추출조건에 따라서 항균활성에 큰 차이를 나타내지 않았으나, 초임계추출물의 경우 추출압력 100 bar, 500 bar 처리구와 추출온도 $35^{\circ}C$, $65^{\circ}C$ 처리구에서 항균활성이 크게 나타났다.

알칼리 추출법에 의한 탈지 미강 단백질 추출의 최적 pH 조건 (Optimum pH Condition of Defatted Rice Protein Extraction by Alkaline Method)

  • 김원;정소영;홍광원
    • 산업식품공학
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    • 제15권2호
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    • pp.143-147
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    • 2011
  • 알칼리를 이용하여 탈지미강에서 단백질을 추출하기 위해 용출 pH(8, 9, 10, 11, 12) 5개 구간과 등전점 침전 pH(2, 4, 6) 3개 구간을 설정하고 각 pH 구간에서의 회수된 단백질 함량과 갈변, 전기영동 패턴 및 최종 수용성 단백질의 회수율을 비교 확인하였다. 용출 pH가 증가할수록 수용성 단백질의 회수율은 증가하였으나 동시에 갈변현상도 증가하였다. 등전점 침전 pH는 4에서 가장 많은 단백질을 회수할 수 있었다. SDS-PAGE 결과, 사용한 pH에 따라 추출된 단백질의 주요 패턴은 뚜렷한 차이가 없었으나, 높은 알칼리조건이 35 kDa 이하의 단백질 추출에 좀 더 용이하였다. 각 pH 구간별 용출 대비 수용성 단백질 회수율은 평균 31.5% 이었다. 용출 pH 11 및 침전 pH 4 구간에서 46.95%의 가장 높은 회수율을 나타냈으나 갈변이 급격히 증가하였다. 따라서 37.65%의 회수율을 나타낸 용출 pH 10 과 침전 pH 4 구간이 미강 단백질의 최적 추출조건임을 확인하였다.

Analytical Method for Dioxin and Organo-Chlorinated Compounds: (Ⅱ) Comparison and Extraction Methods of Dioxins from XAD-2 Adsorbent

  • 양정수;이성광;박영훈;이대운
    • Bulletin of the Korean Chemical Society
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    • 제20권6호
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    • pp.689-695
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    • 1999
  • Supercritical fluid extraction (SFE), ultrasonic extraction (USE), and accelerated solvent extraction (ASE) were compared with the well known Soxhlet extraction for the extraction of polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins(PCDDs) from the XAD-2 resin which was used to adsorb PCDDs in the atmosphere. XAD-2 resin spiked with five PCDDs was chosen as a sample. The optimum conditions for the extraction of PCDDs by SFE were turned out to be the use of CO2 modified with 10% toluene at 100 ℃ and 350 atm, with 5 min static extraction followed by 20 min dynamic extraction. SFE gave a good extraction rate with good reproducibility for PCDDs ranging from 68 to 98%. The ultrasonic extraction of PCDDs from XAD-2 was investigated and compared with other extractions. A probe type method was compared with a bath type. Two extraction solvents, toluene and acetone were compared with their mixture. The use of their mixture in probe type, with 9 minutes of extraction time, was found to be the optimum condition. The average recovery of the five PCDDs for USE was 82-93%. Accelerated solvent extraction (ASE) with a liquid solvent, a new technique for sample preparation, was performed under elevated temperatures and pressures. The effect of tem-perature on the efficiency of ASE was investigated. The extraction time for a 10 g sample was less than 15 min, when the organic solvent was n-hexaneacetone mixture (1 : 1, v/v). Using ASE, the average recoveries of five PCDDs ranged from 90 to 103%. SFE, USE, and ASE were faster and less laborious than Soxhlet extraction. The former three methods required less solvent than Soxhlet extraction. SFE required no concentration of the solvent extracts. SFE and ASE failed to perform simultaneous parallel extractions because of instrumental limitations.

A FINITE ELEMENT METHOD USING SIF FOR CORNER SINGULARITIES WITH AN NEUMANN BOUNDARY CONDITION

  • Kim, Seokchan;Woo, Gyungsoo
    • East Asian mathematical journal
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    • 제33권1호
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    • pp.1-9
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    • 2017
  • In [8] they introduced a new finite element method for accurate numerical solutions of Poisson equations with corner singularities, which is useful for the problem with known stress intensity factor. They consider the Poisson equations with homogeneous Dirichlet boundary condition, compute the finite element solution using standard FEM and use the extraction formula to compute the stress intensity factor, then they pose a PDE with a regular solution by imposing the nonhomogeneous boundary condition using the computed stress intensity factor, which converges with optimal speed. From the solution they could get accurate solution just by adding the singular part. This approach works for the case when we have the reasonably accurate stress intensity factor. In this paper we consider Poisson equations defined on a domain with a concave corner with Neumann boundary conditions. First we compute the stress intensity factor using the extraction formular, then find the regular part of the solution and the solution.