• 제목/요약/키워드: extraction condition

검색결과 1,145건 처리시간 0.026초

Extraction of β-glucosidase from Bagasse Fermented by Mixed Culture under Solid State Fermentation

  • Shata, Hoda Mohamed Abdel Halim;Farid, Mohamed Abdel Fattah
    • Journal of Applied Biological Chemistry
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    • 제57권3호
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    • pp.197-203
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    • 2014
  • Various parameters such as solvent selection, concentration, solid/liquid ratio, soaking time, temperature, stationary, shaking conditions, and repeated extractions were investigated in order to determine the optimum extraction conditions of ${\beta}$-glucosidase from bagasse fermented by mixed culture of Aspergillus niger NRC 7A and Aspergillus oryzae NRRL 447. Among various solvents tested, non ionic detergents gave the best results than the inorganic or organic salt solutions and distilled water. The optimum conditions for extraction of ${\beta}$-glucosidase were 30 min soaking time at $40^{\circ}C$ under shaking condition at 150 rpm, with solid/liquid ratio 1:15 (w/v), which yielded $2882.74{\pm}95.52U/g$ fermented culture (g fc) of enzyme activity. With repeated washes under the above optimum conditions, the results showed that enzyme extracted in the $1^{st}$ and $2^{nd}$ washes represents about 90% of the total activity.

Automatic Extraction of the Interest Organization from Full-Color Continuous Images for a Biological Sample

  • Takemoto, Satoko;Yokota, Hideo;Shimai, Hiroyuki;Makinouchi, Akitake;Mishima, Taketoshi
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2002년도 ITC-CSCC -1
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    • pp.196-199
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    • 2002
  • We presented the automatic extraction technique of a biological internal organization from full-color continuous images. It was implemented using the localized homogeneousness of color intensity, and also using the continuity between neighboring images. Moreover, we set the "four-level status value" of area condition as a value showing "area possibility. This played important role of preventing a miss-judgement of area definition. These our approach had a beneficial effect on tracking color and shape change of the interest area in continuous extraction. As a resell we succeeded in extraction of mouse's stomach from continuous 50 images.

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반응표면분석법에 의한 맥문동 열수추출 조건의 최적화 (Optimization for Hot water Extraction Condition of Liriope spicata Tuber Using Response Surface Methodology)

  • 김순동;구연수;이인자;박인경;윤광섭
    • 한국식품저장유통학회지
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    • 제8권2호
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    • pp.157-163
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    • 2001
  • Optimal conditions for hot water extraction of Liriope spicata tuber were investigated with changes in solvent ratio(2∼6 fold) and heating time(1∼5 hr) by response surface methodology. The content of extractable solids increased with an increased in solvent ratio, and the highest content showed at heating time of 3 hr. The content of total steroid saponin increased with a decrease in solvent ratio, and increased with an increase in heating time at increasing the solvent ratio. The content of non-reducing sugar containing oligosaccharides at a lower solvent ratio didn’t show changes depending on heating time, while that at a higher ration decreased with an increase in heating time. Optimal extraction conditions using hot water as the limited conditions of 15∼18% extractable solids, 1.5∼2.0% total steroid saponin, 6∼8% reducing sugar, 6∼7% non-reducing sugar and 13∼15 brix were 3 hrs of heating time and 4 fold of solvent ratio.

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다차원공간을 이용한 연구테마 기회발굴시스템의 개발 (Development of Opportunity Extraction System for Generating New Research Themes by Using Multi-dimensional Space)

  • 김점복;권철신
    • 한국경영과학회:학술대회논문집
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    • 한국경영과학회 2005년도 추계학술대회 및 정기총회
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    • pp.103-111
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    • 2005
  • In this paper, we have designed a deployment process system that extract and integrate information related to technology and market opportunities through searching for external environment and internal condition on multi-dimensional space in order to generate new research themes. It is expected that this theme extraction model could be contributed to the maximization of R&D productivity by systemization of the opportunity extraction process for generating research themes in R&D center or laboratory.

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임프란트 수술이나 발치 후 지각이상 환자에서의 약물치료 (Pharmacologic management for the patient with paresthesia after implant surgery or extraction)

  • 김성택;김일영;강희석
    • 구강회복응용과학지
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    • 제19권2호
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    • pp.109-113
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    • 2003
  • The inferior alveolar nerve provides unilateral innervation to the dentition, labial mucosa and skin from about commissure to the mental protuberance. Injury to this nerve resulting in sensory impairment can be a distressing problem to some patients. The causes of this problem include trauma, extraction, implant surgery and any maxillofacial surgery and generally the altered sensation is temporary. The surgical procedure has been the most common treatment for this condition but it has some complications. The antidepressants and anticonvulsants have been effective to the treatment of trigeminal dysesthesia. This case report suggests that the use of antidepressants and anticonvulsants is an alternative method to treat the paresthesia after implant surgery or extraction.

은행잎 플라보놀배당체에 대한 셀루라제류의 영향 (The Effect of Cellulases on Flavonolglycosides of Ginkgo Leaf)

  • 배기환;민병선;백흠영;안병준
    • 약학회지
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    • 제35권4호
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    • pp.271-276
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    • 1991
  • The extractability and stability of ginkgoflavonolglycosides under presence of several cellulose preparations were investigated. The enzymes used were macerosin, cellulose C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglycosides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at $40^{\circ}C$, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of $\beta$-glycosidase and $\alpha$-rhamnosidase. Kaempferol-3-O-(6'"-O-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition.tion.

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강황에서 curcuminoids의 고순도 추출 및 고성능 액체 크로마토그래피 동시분석 (High Purity Extraction and Simultaneous High-performance Liquid Chromatography Analysis of Curcuminoids in Turmeric)

  • 이광진;마진열;김영식;김동선;김은철
    • Journal of Applied Biological Chemistry
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    • 제55권1호
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    • pp.61-65
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    • 2012
  • Three major curcuminoids in turmeric (Curcuma longa), curcumin, demethoxycurcumin, and bisdemethoxycurcumin, were efficiently extracted by optimizing extraction condition and simultaneously analyzed and identified using reverse phase high-performance liquid chromatography, thin-layer chromatography, and liquid chromatography-mass spectrometry method. The highest yield of extraction amount 0.279 g, 9.30% was obtained by dipping method with extraction time of 7 h.

소목 염료의 추출조건이 색상에 미치는 영향 I (Color Changes according to the Extraction Condition of Caesalpinia sappan Dyestuff I)

  • 전희영;최세민;안정훈;전동원
    • 패션비즈니스
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    • 제13권2호
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    • pp.136-144
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    • 2009
  • The purpose of this study is to investigate various phenomena quantitatively occurring during the course of changing dyeing conditions of cotton and silk fibers in natural dyeing using Caesalpinia sappan. Paying attention to the fact that the color may be varied according to the extraction conditions of dyestuffs in Caesalpinia sappan dyeing, the color changes were investigated after dyeing using dyestuffs extracted at elevated temperature and dyestuffs extracted at room temperature. According to the extraction methods, the degree of color development for the category of red color and the category of yellow color has changed.

보리차의 추출 조작 관련 물성에 관하여 (Physical Properties and Rheology Effecting on Barley Tea Extraction)

  • 박상기;전재근
    • 한국식품영양학회지
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    • 제4권1호
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    • pp.61-68
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    • 1991
  • In order to study the extraction mechanism of barley tea, some factors affecting extraction and physical properties of roasted barley and extract were examined. The main results of this study are as follows : 1. The roasted barley particles used as the raw materials were amorphous. Under the extraction condition used the average swelling ratio of the particles and imbibed volume were 1. 3g and 1.65ml/g respectively. The maximum soluble solid content in the roasted barley was 67.07%. 2. The concentrations of the barley extracts were determined by measuring their optical densitis at 420nm and the relationship between the concentration(C) and optical density(O. D.) was expressed as : C=6, 178XO. D. As shown in the following relationship, the viscosities(u: Ns/m2) of barley extracts were Influenced by concentration(C : kg/m3) and temperature(T : C). $\mu$=5.6731$\times$$10^{-4}e^{2.7}^{\times10^{{-3}\times{C}}$$\mu$=3.577$\times$$10^{-3}e^{{-3.02}\times{10^{-2}\times{T}}$

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Study on A Laser-induced Photoredox Reaction for the Extraction of Precious Elements from Aqueous Solutions

  • Kyuseok Song;Hyungki Cha;Lee, Jongmin;Park, Jongsoo;Lee, Yong-Ill
    • Nuclear Engineering and Technology
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    • 제32권6호
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    • pp.531-536
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    • 2000
  • The extraction of precious metals from aqueous solutions is performed by using a photoredox reaction with a Q-switched Nd:YAG laser. The metallic silver was efficiently precipitated and extracted from the silver nitrate solution by laser photolysis. An optimum reaction condition for silver extraction was determined by adjusting various experimental factors such as type of reducing agent, type of acids and reaction time. The composition of the reaction product was analyzed and it was identified as metallic silver, not other molecular types. The photoreaction of chromium(III) chloride in an acidic aqueous solution was also investigated. The 355 nm laser light was better suited for the reaction of silver nitrate as well as chromium(III) chloride in an acidic solution compared to the 532 nm light.

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