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Physicochemical characteristics and physiological activities of mixture extracts from Liriope platyphylla, Schizandra chinensis, and Panax ginseng C.A. Meyer (맥문동, 오미자 및 인삼 혼합추출물의 이화학적 특성 및 생리활성)

  • Gu, Yul-Ri;Hong, Joo-Heon
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.431-439
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    • 2017
  • This study was conducted to examine the antioxidant activities and physiological activities of mixture extracts (Liriope platyphylla, Schizandra chinensis and Panax ginseng C.A. Meyer) with different extraction mixing ratios (MEC, 2:1:1; ME1, 1:2:1; ME2, 1:1:2; ME3, 1.34:1.33:1.33). The yield of extracts ranged from 25.33 to 33.87%. The total polyphenol and total flavonoid contents of ME1 extracts were 1.01 g/100 g, 0.07 g/100 g, respectively. The total sugar contents of MEC extract was 22.83 g/100 g, respectively. The DPPH and ABTS radical scavenging activities of ME1 extracts at $1,000{\mu}g/mL$ were 26.79% and 21.08%. The superoxide radical scavenging and ferric-reducing antioxidant power of ME1 extracts at $1,000{\mu}g/mL$ were 67.83% and $295.47{\mu}M$, respectively. The functionalities of extracts were investigated with L-132 and RAW264.7 cell lines. The extracts on different mixing ratios did not show the toxicity on L-132 and RAW264.7 cell line in $100-2,500{\mu}g/mL$. The ME1 extract of $1,000{\mu}g/mL$ performed better than other extracts protective effects against oxidative stess in L-132 cells (81.22%) and the ME2 extract at $1,000{\mu}g/mL$ decreased nitric oxide production by $7.48{\mu}M$ which was more potent than other extracts. There results suggest that the ME1 extracts may be a useful functional food material in the food industry.

Antioxidant and anti-inflammatory effects of red garlic compositions (홍마늘 조성물의 항산화 및 항염증 효과)

  • Kang, Min Jung;Kim, Dong-Gyu;Shin, Jung Hye
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.446-454
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    • 2017
  • Garlic (Allium sativum L.) and traditional herb has several functional properties and strong biological activities, making it useful as a functional food material. We investigated the antioxidant and anti-inflammatory activity of mixed compounds from red garlic and supplementary materials, including ginger (Zingiber officinale Roscoe), doraji (Platycodon grandiflorum), quince (Chaenomeles sinensis), citrus peel (Citri Pericarpium), and mint (Mentha arvensis). The extracts were prepared with water (W) and ethanol (E) at $70^{\circ}C$ (W-70, E-70) and $95^{\circ}C$ (W-95, E-95) for 3 h. The total content of phenolic compounds was the highest in E-70 (608.60 mg/100 g). Alliin, one of the active ingredients in red garlic, was contained at 1.18-1.29 mg/g and 0.81-0.85 mg/g in water and ethanol extract, respectively. Another active ingredient of red garlic, S-allyl-cysteine (SAC) had higher content in the water extract than in the ethanol extracts. DPPH radical scavenging activity was higher in E-70 (15.96-73.65%) at $313-5,000{\mu}g/mL$. ABTS radical scavenging activity was also higher in E-70 (5.71-77.19%) than in the others. The ROS production rate showed the same tendency as the NO production, with more efficacy in E-95. The expression level of iNOS and $IL-1{\beta}$ was decreased in the E-95 significantly at the concentration of $1,000{\mu}g/mL$ compared to the lipopolysaccharide (LPS) treated group. Based on the above results, the antioxidative and anti-inflammatory activities of the extracts of red garlic and supplementary materials were expressed by different useful substances. The contents of these useful substances were different according to the extraction solvent and temperature.

Comparison of volatile flavor compounds of yuzu, kumquat, lemon and lime (유자, 금귤, 레몬 및 라임의 휘발성 향기성분의 비교)

  • Hong, Young Shin;Lee, Ym Shik;Kim, Kyong Su
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.394-405
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    • 2017
  • This study was conducted to confirm the usefulness of essential oil components in yuzu and kumquat cultivated in Korea for comparison with those in lemon and lime. The volatile flavor compounds in citrus fruits (yuzu, kumquat, lemon and lime) were extracted for 3 h with 100 mL redistilled n-pentane/diethylether (1:1, v/v) mixture, using a simultaneous steam distillation and extraction apparatus (SDE). The volatile flavor compositions of the samples were analyzed by gas chromatography-mass spectrometry (GC-MS). The aroma compounds analyzed were 104 (3,713.02 mg/kg) in yuzu, 87 (621.71 mg/kg) in kumquat 103 (3,024.69 mg/kg) in lemon and 106 (2,209.16 mg/kg) in lime. Limonene was a major volatile flavor compound in four citrus fruits. The peak area of limonene was 35.03% in yuzu, 63.82% in kumquat, 40.35% in lemon, and 25.06% in lime. In addition to limonene, the major volatile flavor compounds were ${\gamma}$-terpinene, linalool, ${\beta}$-myrcene, (E)-${\beta}$-farnesene, ${\alpha}$-pinene and ${\beta}$-pinene in yuzu, and ${\beta}$-myrcene, ${\alpha}$-pinene, (Z)-limonene oxide, (E)-limonene oxide, geranyl acetate and limonen-10-yl acetate in kumquat. Furthermore, ${\gamma}$-terpinene, ${\beta}$-pinene, ${\beta}$-myrcene, geranyl acetate, neryl acetate and (Z)-${\beta}$-bisabolene in lemon and ${\gamma}$-terpinene, ${\beta}$-pinene, (Z)-${\beta}$-bisabolene, neral, geranial and neryl acetate in lime were also detected. As a result, it was confirmed that the composition of volatile flavor compounds in four citrus fruits was different. Also, yuzu and kumquat are judged to be worthy of use alternatives for lemon and lime widely used in the fragrance industry.

Physicochemical properties and antioxidant activities of Streptococcus thermophilus KCCM 3782 strain fermented Cordyceps militaris grown on Tenebrio molitor (갈색거저리 동충하초의 Streptococcus thermophilus KCCM 3782 유산균 발효에 따른 물리화학적 특성과 산화방지 활성 비교)

  • Gim, Do-Woo;Park, Jun-Ki;Kim, Hyun-Jin;Cho, In-Kyung;Cho, Ja-Yong;Kim, Yong-Doo;Huh, Chang-Ki
    • Food Science and Preservation
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    • v.25 no.1
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    • pp.136-144
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    • 2018
  • This study aimed to provide basic evidence regarding the development of materials by analyzing the physicochemical properties and antioxidant activities of Streptococcus thermophilus KCCM 3782 strain-fermented Cordyceps militaris grown on Tenebrio molitor. Among the solvents tested, DPPH radical scavenging activity was the highest in the hot water-extracted sample after 30 min of extraction. Moisture content decreased, whereas crude protein and fat content increased, after lactic acid bacteria-mediated fermentation. Sodium, magnesium, calcium, and zinc contents increased, with potassium levels attaining the maximum value, whereas free amino acid content decreased after the fermentation. Among Hunter's color values, a value increased to $66.7{\rightarrow}149.92$ after fermentation, whereas the L and b values decreased to $15.79{\rightarrow}-15.75$ and $54.45{\rightarrow}0.01$, respectively. Cordycepin content assay increased from 7.02 mg% to 8.66 mg%. The DPPH free radical scavenging activity of the fermented product was 91.92 EDA%, which was higher than that of the extract (84.69 EDA%). The ABTS free radical scavenging and superoxide dismutase (SOD) activities were also higher in the fermented products.

Effect of antioxidation and antibacterial activity on crude extract and Characterization of American Cockroaches (Periplaneta americana L.) in Korea (국내 서식 미국바퀴(Periplaneta americana L.)의 특성 및 추출물의 항산화·항균 효과)

  • Kim, Jung-Eun;Kim, Seon-Gon;Kang, Sung-Ju;Kim, Chun-Sung;Choi, Yong-Soo
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.135-142
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    • 2015
  • The American cockroaches, Periplaneta americana L. was the most important worldwide pest species. It has been an public health problems. We were determinated life cycle and extraction of crude extracts by chemical reagents from cockraches (P. americana L.). The extracted crude solution has been antibacterial activity to gram negative bacteria (Pseudomonas aeruginosa, $6.44{\pm}1.03mm$), gram positive bacteria (Bacillus subtilis, $1.88{\pm}0.40mm$), and fungus (Candida albicans, $5.61{\pm}0.57mm$) using radial diffusion assay. We were analysed of up-regulation of Glutathione-S-transferases (GSTs) stimulation, indicating that antioxidantial protein from various classes are simultaneously expressed in a single insect upon infection or injury. The gene from Periplaneta americana L. were cloned, analysed sequence, and measured protein expression by Real Time PCR (Polymerase Chain Reaction).

Analysis of Fungicide Prochloraz in Platycodi Radix by GC-ECD (GC-ECD를 이용한 한약재 길경(Platycodi Radix) 중 살균제 Prochloraz의 분석)

  • Oh, Gyeong-Seok;Yoon, Myung-sub;Yang, Seung-Hyun;Choi, Hoon
    • Korean Journal of Environmental Agriculture
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    • v.40 no.4
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    • pp.353-358
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    • 2021
  • BACKGROUND: Prochloraz has been widely used as an imidazole fungicide on fruits and vegetables in Korea. Analytical approaches to evaluate prochloraz residues in herbal medicine are required for their safety management. In this study, we developed a GC-ECD method for quantitative determination of prochloraz in Platycodi Radix. The metabolite 2,4,6-trichlorophenol (2,4,6-T) was used as a target compound to evaluate total prochloraz residues as it is categorized to a representative residue definition of prochloraz. All residues containing 2,4,6-T were converted to 2,4,6-T and subjected to GC-ECD. METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining prochloraz and it metabolite 2,4,6-T in Platycodi Radix. Prochloraz and its metabolite 2,4,6-T residuals were extracted using acetone. The extract was diluted with and partitioned directly into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was decomposed to 2,4,6-T, and then the partitioned ion-associate was finally purified by optimized aminopropyl solid-phase extraction (SPE). The limits of quantitation of the method (MLOQs) were 0.04 mg/kg and 0.02 mg/kg, respectively for prochloraz and 2,4,6-T, considering the maximum residue level (MRL) of prochloraz as 0.05 mg/kg in Platycodi Radix. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (82.1-89.7%). Good reproducibilities were obtained (coefficient of variation < 2.8%), and the linearities of calibration curves were reasonable (r2 > 0.9986) in the range of 0.005-0.5 ㎍/mL. CONCLUSION(S): The method developed in this study was successfully validated to meet the guidelines required for quantitative determination of pesticides in herbal medicine. Thus, the method could be useful to monitor prochloraz institutionally in herbal medicine.

Aggregate of Korea in 2020 (2020년도 국내 골재 수급 분석)

  • Hong, Sei Sun;Lee, Jin Young
    • Economic and Environmental Geology
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    • v.54 no.5
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    • pp.581-594
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    • 2021
  • In 2020, about 132 million m3 of aggregate was produced in Korea. Of the total domestic aggregates produced in 2020, about 33.3 % was sand and about 66.7% was gravel. It estimated that of the 132 million m3 of aggregates in Korea in 2020, about 52% was produced by screening crushed aggregate, by 36% by forest aggregate, 3% by land aggregate, 5.6% by sea aggregate and 2.5% by washing each other, and 0.4% by river aggregate. This indicates that screening crushed aggregate and forest aggregate are the main producers of domestic aggregates. Leading producing metropolitan cities were Gyeonggi-do, Gyeongsangnam-do, Chungcheongbuk-do, Gangwon-do, Chungcheongnam-do, Incheon in order decreasing volume, which together accounted for about 72.4% of total product. In 2020, aggregates were produced in 153 cities, about 67% of the 231 cities of Korea, 38 local governments have developed aggregates of more than 1 million m3, and the combined production of the 38 cities accounted for about 65% of national total. This means that the aggregate extraction trend of local governments is becoming larger and more concentrated. In 2020, at 153 local governments, a total of 889 operations produced aggregates with 420 operations by permission, 469 operations by declaration. A review of production by size of operation indicated that about 17 million m3 (12.8% of the total aggregate) was produced by 14 operations reporting production of more than 1 million m3. In about 420 operations, the maximum period of permit is 32 years to at least 2 months. When the remaining period of permit is taken into account, only about 55% of active operations can be developed the aggregate after 2021. In order to maintain the permitted aggregate volume by 2020 level, it will be necessary to obtain an extension permit or find new operation sites for at least 200 or more operations.

Development of Cloud and Shadow Detection Algorithm for Periodic Composite of Sentinel-2A/B Satellite Images (Sentinel-2A/B 위성영상의 주기합성을 위한 구름 및 구름 그림자 탐지 기법 개발)

  • Kim, Sun-Hwa;Eun, Jeong
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.989-998
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    • 2021
  • In the utilization of optical satellite imagery, which is greatly affected by clouds, periodic composite technique is a useful method to minimize the influence of clouds. Recently, a technique for selecting the optimal pixel that is least affected by the cloud and shadow during a certain period by directly inputting cloud and cloud shadow information during period compositing has been proposed. Accurate extraction of clouds and cloud shadowsis essential in order to derive optimal composite results. Also, in the case of an surface targets where spectral information is important, such as crops, the loss of spectral information should be minimized during cloud-free compositing. In thisstudy, clouds using two spectral indicators (Haze Optimized Tranformation and MeanVis) were used to derive a detection technique with low loss ofspectral information while maintaining high detection accuracy of clouds and cloud shadowsfor cabbage fieldsin the highlands of Gangwon-do. These detection results were compared and analyzed with cloud and cloud shadow information provided by Sentinel-2A/B. As a result of analyzing data from 2019 to 2021, cloud information from Sentinel-2A/B satellites showed detection accuracy with an F1 value of 0.91, but bright artifacts were falsely detected as clouds. On the other hand, the cloud detection result obtained by applying the threshold (=0.05) to the HOT showed relatively low detection accuracy (F1=0.72), but the loss ofspectral information was minimized due to the small number of false positives. In the case of cloud shadows, only minimal shadows were detected in the Sentinel-2A/B additional layer, but when a threshold (= 0.015) was applied to MeanVis, cloud shadowsthat could be distinguished from the topographically generated shadows could be detected. By inputting spectral indicators-based cloud and shadow information,stable monthly cloud-free composited vegetation index results were obtained, and in the future, high-accuracy cloud information of Sentinel-2A/B will be input to periodic cloud-free composite for comparison.

Analysis of Polar Region-Related Topics in Domestic and Foreign Textbooks (국내외 교과서에 수록된 극지 관련 내용 분석)

  • Chung, Sueim;Choi, Haneul;Choi, Youngjin;Kang, Hyeonji;Jeon, Jooyoung;Shin, Donghee
    • Journal of the Korean earth science society
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    • v.42 no.2
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    • pp.201-220
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    • 2021
  • The objective of this study is to increase awareness and interest regarding polar science and thereby aid in establishing the concept and future direction of polar literacy. To analyze the current status, textbooks based on the common school curriculum pertaining to polar topics were reviewed. Six countries that actively conduct polar science, namely Korea, France, Japan, Germany, the United States, and the United Kingdom, were chosen. Subsequently, 402 cases in 110 science and social studies (geography) textbooks of these countries were analyzed through both quantitative and qualitative methods. Based on the obtained results, the importance of polar research in geoscience education and the need for spreading awareness regarding polar research as an indicator of global environmental changes were examined. It was found that the primary polar topics described in the textbooks are polar glaciers, polar volcanism, solid geophysics, polar infrastructure, and preservation of geological resources and heritage. This demonstrates that the polar region is a field of research with important clues to Earth's past, present, and future environments and is also a good teaching subject for geological education. However, an educational approach is needed for systematically laying emphasis on polar research. The implications of this study are manifold, such as the establishment of a cooperative system between polar scientists and educators, extraction of core concepts for polar literacy and content reconstruction, discovery of new polar topics associated with the curriculum, diversification of forms of presentation in textbooks, and development of an affective image that is based on correct cognitive understanding. Furthermore, through the continuance of polar topics in textbooks, students can improve their awareness regarding polar literacy and polar science culture, which in turn will serve as the driving force for sustainable polar research in the future.

Monitoring of a Time-series of Land Subsidence in Mexico City Using Space-based Synthetic Aperture Radar Observations (인공위성 영상레이더를 이용한 멕시코시티 시계열 지반침하 관측)

  • Ju, Jeongheon;Hong, Sang-Hoon
    • Korean Journal of Remote Sensing
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    • v.37 no.6_1
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    • pp.1657-1667
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    • 2021
  • Anthropogenic activities and natural processes have been causes of land subsidence which is sudden sinking or gradual settlement of the earth's solid surface. Mexico City, the capital of Mexico, is one of the most severe land subsidence areas which are resulted from excessive groundwater extraction. Because groundwater is the primary water resource occupies almost 70% of total water usage in the city. Traditional terrestrial observations like the Global Navigation Satellite System (GNSS) or leveling survey have been preferred to measure land subsidence accurately. Although the GNSS observations have highly accurate information of the surfaces' displacement with a very high temporal resolution, it has often been limited due to its sparse spatial resolution and highly time-consuming and high cost. However, space-based synthetic aperture radar (SAR) interferometry has been widely used as a powerful tool to monitor surfaces' displacement with high spatial resolution and high accuracy from mm to cm-scale, regardless of day-or-night and weather conditions. In this paper, advanced interferometric approaches have been applied to get a time-series of land subsidence of Mexico City using four-year-long twenty ALOS PALSAR L-band observations acquired from Feb-11, 2007 to Feb-22, 2011. We utilized persistent scatterer interferometry (PSI) and small baseline subset (SBAS) techniques to suppress atmospheric artifacts and topography errors. The results show that the maximum subsidence rates of the PSI and SBAS method were -29.5 cm/year and -27.0 cm/year, respectively. In addition, we discuss the different subsidence rates where the study area is discriminated into three districts according to distinctive geotechnical characteristics. The significant subsidence rate occurred in the lacustrine sediments with higher compressibility than harder bedrock.