• 대한간호학회지
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• 제34권7호
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• pp.1234-1242
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• 2004

Purpose: This cross-sectional study was designed to identify anger-expression types of adolescent women and investigate the relation between the identified anger-expression types and their problem behaviors and health status. Method: One hundred ninety nine high school freshmen were recruited from September to November, 2003. Data was analyzed using descriptive statistics, cluster analysis, 2-test, ANOVA, and Duncan's multiple comparison test. Result: Three anger-expression types in adolescent women were found; Anger-out/in, Anger-control/in, and Anger-control type. Adolescent women with frequently using the anger-out/in type and with higher state anger reported more delinquent behaviors, more health risk behaviors, and higher psychosomatic symptoms. However, adolescent women with lower state anger and frequently using the anger-control type reported more depression scores. Conclusion: There is a need to further clarify the relationship between anger-expression type sand depression in adolescent women. The findings suggest the necessity of a development of the program for lowering the anger level and controlling the unfavorable anger expression types such as the anger-out in.

• 대한의생명과학회지
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• 제15권2호
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• pp.113-118
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• 2009

Actin cytoskeletal architecture is believed to be a crucially important modulator of chondrocyte phenotype. 2DG(2-Dexoy-D-glucose) induces reorganization of actin cytoskeletal architecture in chondrocytes. In this study, we have investigated the effects of 2DG on dedifferentiation and inflammation via reorganization of cytoskeletal architecture in rabbit articular chondrocytes, with a focus on p38 kinase pathway. Treatment of 2DG alone reduced type II collagen and COX-2 expression in chondrocytes. But, 2DG reduced type II collagen was recovered by CD, disruptor of actin cytoskeletal architecture, whereas did not affect on COX-2 expression and production of $PGE_2$ compared with 2DG alone treated cells. Treatment of 2DG with JAS, inducer of cytoskeletal architecture polymerization, accelerated reduction of type II collagen expression and synthesis of proteoglycan but did not affect on COX-2 expression and production of $PGE_2$. Also, 2DG stimulated activation of p38 kinase. This result showed that 2DG regulates type II collagen but not cyclooxygenase-2 expression through reorganization of cytoskeletal architecture via p38 kinase pathway in rabbit articular chondrocytes.

• 여성건강간호학회지
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• 제21권2호
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• pp.128-138
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• 2015

Purpose: This cross-sectional study was designed to identify anger-expression types of adolescent women and investigate the relationship between the identified anger-expression types and their problem behaviors and suicide probability. Methods: The participants were 942 students at two female high school located in Gwangju. The data were analyzed using descriptive statistics, cluster analysis, t-test, ANOVA, and $Scheff{\acute{e}}$ multiple comparison test. Results: Cluster analysis revealed 4 distinct anger expression types; Anger-out, Low anger expression, Anger-control, and Anger-in/out types. Female adolescent women had a higher level of trait anger or who frequently used the anger-in/out type reported internalized-externalized problem behaviors and suicide probability more frequently compared to those who frequently used the other three types of anger expression. Conclusion: Female adolescent women who had the low anger expression type and anger control type managed anger most effectively. The findings suggest the necessity of a development of the program for lowering the trait anger level and controlling the unfavorable anger expression types such as the anger-in/out.

• Genomics & Informatics
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• 제18권3호
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• pp.26.1-26.6
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• 2020

Single-cell RNA sequencing (scRNA-seq) has been widely applied to provide insights into the cell-by-cell expression difference in a given bulk sample. Accordingly, numerous analysis methods have been developed. As it involves simultaneous analyses of many cell and genes, efficiency of the methods is crucial. The conventional cell type annotation method is laborious and subjective. Here we propose a semi-automatic method that calculates a normalized score for each cell type based on user-supplied cell type-specific marker gene list. The method was applied to a publicly available scRNA-seq data of mouse cardiac non-myocyte cell pool. Annotating the 35 t-stochastic neighbor embedding clusters into 12 cell types was straightforward, and its accuracy was evaluated by constructing co-expression network for each cell type. Gene Ontology analysis was congruent with the annotated cell type and the corollary regulatory network analysis showed upstream transcription factors that have well supported literature evidences. The source code is available as an R script upon request.

• 한국인터넷방송통신학회논문지
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• 제11권1호
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• pp.177-183
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• 2011

수식의 자료형 확인을 위하여 많이 사용하는 방법의 하나가 속성문법이다, 그런데 이 속성문법은 구성하기가 어려운 단점이 있다. 파싱 과정을 잘 이해하고 속성문법의 두 개의 속성들을 사용하여야 하기 때문이다. 그래서 본 논문에서는 이 수식 속성문법의 구성을 보다 쉽게 하기 위한 방법을 제시한다. 문법 구성을 통하여 하나의 속성만으로 수식의 자료형 확인이 가능함을 보여준다.

• IMMUNE NETWORK
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• 제20권5호
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• pp.39.1-39.13
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• 2020

Sjögren's syndrome (SS) is a chronic and systemic autoimmune disease characterized by lymphocytic infiltration in the exocrine glands. In SS, type I IFN has a pathogenic role, and recently, inflammasome activation has been observed in both immune and non-immune cells. However, the relationship between type I IFN and inflammasome-associated pyroptosis in SS has not been studied. We measured IL-18, caspase-1, and IFN-stimulated gene 15 (ISG15) in saliva and serum, and compared whether the expression levels of inflammasome and pyroptosis components, including absent in melanoma 2 (AIM2), NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein (ASC), caspase-1, gasdermin D (GSDMD), and gasdermin E (GSDME), in minor salivary gland (MSG) are related to the expression levels of type I IFN signature genes. Expression of type I IFN signature genes was correlated with mRNA levels of caspase-1 and GSDMD in MSG. In confocal analysis, the expression of caspase-1 and GSDMD was higher in salivary gland epithelial cells (SGECs) from SS patients. In the type I IFN-treated human salivary gland epithelial cell line, the expression of caspase-1 and GSDMD was increased, and pyroptosis was accelerated in a caspase-dependent manner upon inflammasome activation. In conclusion, we demonstrate that type I IFN may contribute to inflammasome-associated pyroptosis of the SGECs of SS patients, suggesting another pathogenic role of type I IFN in SS in terms of target tissue -SGECs destruction.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.789-793
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• 1999

The effect of acetic acid formation deficiency on recombinant E. coli fermentation was investigated using a mutant strain deficient in acetic acid formation. A mutant strain which does not grow under anaerobic conditions was isolated. The acetic acid production in this strain was negligible in aerobic batch fermentation. The cloned-gene expression in the mutant strain was higher than the wild-type strain. Fed-batch fermentations with controlled specific growth rates were carried out in order to compare the cloned-gene expression between the wild-type and the mutant strains. The expression decreased along with the specific growth rate in both strains. The cloned-gene expression in the mutant strain was 60% higher than in the wild-type strain at the same specific growth rate.

• 대한바이러스학회지
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• 제29권3호
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• pp.183-193
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• 1999

Human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein is synthesized as a 160 KDa precursor, gp160, that is cleaved by a cellular protease to form the gp120 and gp41 subunits. Mammalian expression vectors were designed that are capable of efficient expression of various mutant envelope glycoproteins derived from a molecular clone of HIV-1. To construct these vectors, one type of mutation was made at the gp120-gp41 cleavage site by oligonucleotide-directed mutagenesis. And another mutation was made to change amino acids in the membrane spanning region of HIV-1 gp41 important for membrane anchorage. Next, these two mutations were combined to generate a vector to have double mutations in cleavage site and membrane-spanning region. These mutants were transiently expressed in mammalian cells. The effect of these mutations on envelope glycoprotein synthesis, proteolytic processing and secretion was determined. In addition, cell surface expression and ability of the glycoprotein to induce syncytium formation were examined. This study provides a mammalian expression system that is capable of efficient expression and secretion of soluble gp160.

• Journal of Gastric Cancer
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• 제4권2호
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• pp.82-88
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• 2004

Purpose: E-cadherin and CD44H have been shown to play a role in the progression and the metastasis of tumors. This study evaluated the clinical correlations between expression of E-cadherin and CD44H and various clinicopathologic factors and the value of expressions of E-cadherin and CD44H as prognostic factors in Borrmann type IV gastric cancer. Materials and Methods: In 122 patients with Borrmann type IV gastric cancer, we performed the immunohistochemical stainings for E-cadherin and CD44H. We analyzed the correlation between the expressions of E-cadherin and CD44H and lymphatic invasion, venous invasion, perineural invasion, histologic type, lymph node metastasis, depth of invasion, stage, and peritoneal dissemination, and survival. Results: There were no correlations between reduced expression of E-cadherin and CD44H and lymphatic invasion, venous invasion, perineural invasion, histologic type, lymph node metastasis, depth of invasion, and stage. However, there was a significant correlation between lymph node metastasis and the lymphatic invasion (P=0.022). There was also a significant correlation between the peritoneal dissemination and CD44H expression (P=0.005). The 5-year survival rate was correlated with CD44H expression expression (P=0.026), peritoneal dissemination (P<0.01), depth of invasion (P<0.01), lymph node metastasis (P<0.01), stage of tumor (P<0.01), and lymphatic invasion (P<0.01). There was no correlation between expression of E-cadherin and survival rate. Conclusion: The expression of CD44H and peritoneal dissemination was correlated. The expression of CD44H was an independent prognostic factor in Borrmann type IV gastric cancer. Further prospective studies with a large number of cases are required.

• 복식
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• 제57권4호
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• pp.30-44
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• 2007

This research searched characteristic of expression of Surrealism in Fashion Art which is new genre as source of design origination and classified as type of Time, Instinct, Existence and analyzed these and after analyzed result, it tried to verify the value aesthetically and followed by compared characteristic of expression in Surrealism appeared in Art based on content of type of expression that deduced and discussed it. Also comparing common characteristic appeared in Fashion Art in surrealism of the east and the west, it suggested discussion that try to find out identity that draw more near to Fashion Art. Results of research are as following these; Surrealist deliver sentiment and emotion that impossible to happen in reality and primitive thought by combination of unrealistic image and this tendency concreted by expression of Time, Expression of Instinct, expression of Existence. It appeared that deconstruct function and purpose of Fashion Art and existing traditional beauty of form and it is in collusion with way of expression of modelling used in Art of Surrealism those are Irregularity, Disorder, Imperfection and Dissymmetry.