• 정보처리학회논문지A
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• 제14A권2호
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• pp.85-90
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• 2007

본 논문은 애니메이터로 하여금 표정공간으로부터 임의의 표정상태 수 개를 선택하도록 하면, 최적의 표정전이경로를 자동적으로 설정하도록 해줌으로써, 얼굴 표정 애니메이션을 실시간적으로 생성하거나 표정 제어가 가능하도록 하기 위한 기법을 기술한다. 표정공간은 약 2500개의 얼굴 표정상태 간의 거리를 구하고, 다차원 스케일링 기법을 사용하여 2차원 평면에 분포시킴으로서 형성된다. 표정공간에서 최적의 표정전이경로를 설정하기 위해서는 임의의 얼굴 표정상태를 기준으로 사분면처럼 4개의 영역으로 나눈다. 그리고 각 영역별로 최단거리에 존재하는 열굴 표정상태를 결정하고, 그 중에서 가장 가까운 얼굴 표정상태를 선택하여 전이시키고, 전이가 끊어진 얼굴 표정상태에서는 두 번째, 세 번째 혹은 네 번째로 가까운 얼굴 표정상태를 선택하여 순서대로 전이시킴으로써 완전한 표정전이경로가 결정된다. 그리고 애니메이터가 표정공간에서 대표적인 수 개의 표정상태만을 선택해주면 시스템은 자동적으로 최적의 표정전이경로를 설정하여 준다. 본 논문은 애니메이터들로 하여금 본 시스템을 사용하여 얼굴 애니메이션을 생성하거나 표정 제어를 수행하도록 하였으며, 그 결과를 평가한다.

• 한국실내디자인학회논문집
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• 제14권6호
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• pp.168-176
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• 2005

This study is a understanding on technological specific expression to reflect of a research institute in modern architecture. For this study, it separately reduce space program, plan program, design elements of elevation, structural system, mechanical system, building material program to its expressional elements of research institute. Also, it's arranged about a specificity that is expressed for technology each plan elements of a research institute. As a result, elements what is expressive of technology on research institute are influenced an aesthetic expression, expression of an arrangement system, structural expression, expression of architectural organization, and it influences more getting feels technological specificity expressed an aesthetic, structural system, architectural composition, mechanical distribution system in order of their magnitude. This is offered suggestion what must be an achievable complex both a simply technological expression and aesthetic expression.

• 한국콘텐츠학회논문지
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• 제8권2호
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• pp.18-26
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• 2008

본 논문은 사용자로 하여금 표정공간으로부터 일련의 표정들을 선택하게 함으로써 3차원 아바타의 표정을 제어할 수 있는 표정공간의 단계적 가시화 기법을 기술한다. 본 기법에 의한 시스템은 무표정 상태를 포함하여 11개의 서로 다른 모션들로 구성된 2400여개의 표정 프레임으로 2차원 표정공간을 구성하였으며, 3차원 아바타의 표정 제어는 사용자가 표정공간을 항해함으로서 수행되어진다. 그러나 표정공간에서는 과격한 표정 변화에서부터 세밀한 표정 변화까지 다양한 표정 제어를 수행할 수 있어야하기 때문에 단계적 가시화 기법이 필요하다. 표정공간을 단계적으로 가시화하기 위해서는 퍼지 클러스터링을 이용한다. 초기 단계에서는 11개의 클러스터 센터를 가지도록 클러스터링하고, 단계가 증가될 때 마다 클러스터 센터의 수를 두 배씩 증가시켜 표정들을 클러스터링한다. 이때 클러스터 센터와 표정공간에 분포된 표정들의 위치는 서로 다른 경우가 많기 때문에, 클러스터 센터에서 가장 가까운 표정상태를 찾아 클러스터 센터로 간주한다. 본 논문은 본 시스템이 어떤 효과가 있는지를 알기 위해 사용자들로 하여금 본 시스템을 사용하여 3차원 아바타의 단계적 표정 제어를 수행하게 하였으며, 그 결과를 평가한다.

• Journal of Genetic Medicine
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• 제3권1호
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• pp.33-37
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• 1999

Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expression level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.

• Reproductive and Developmental Biology
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• 제36권1호
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

• 제어로봇시스템학회논문지
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• 제12권3호
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• pp.255-263
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• 2006

In the last decade, face analysis, e.g. face detection, face recognition, facial expression recognition, is a very lively and expanding research field. As computer animated agents and robots bring a social dimension to human computer interaction, interest in this research field is increasing rapidly. In this paper, we introduce an artificial emotion mimic system which can recognize human facial expressions and also generate the recognized facial expression. In order to recognize human facial expression in real-time, we propose a facial expression classification method that is performed by weak classifiers obtained by using new rectangular feature types. In addition, we make the artificial facial expression using the developed robotic system based on biological observation. Finally, experimental results of facial expression recognition and generation are shown for the validity of our robotic system.

• Reproductive and Developmental Biology
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• 제37권3호
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• pp.109-115
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• 2013

Until recently the most popular tetracycline-inducible gene expression system has been the one developed by Gossen and Bujard. In this study, we tested the latest version of same system and the results are summarized as follows: Compared with previous one, the difference of new system are minor changes of nucleotide sequences in transactivator and tetracycline response element (TRE) regions. Sensitivity to the doxycycline (a tetracycline derivative) was improved. Leakiness of GFP marker gene expression in non-inducible condition was significantly decreased. Higher expression of the marker gene was observed when the cells were fed with doxycycline-containing medium. Optimal insertion site of woodchuck posttranscriptional regulatory element (WPRE) sequence which was known to increase gene expression was different depending on the origin of cells. In chicken embryonic fibroblast, location of WPRE sequence at 3' end of TRE resulted in the highest GFP expression. In bovine embryonic fibroblasts, 3' end of transactivator was the best site for the GFP expression.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1124-1131
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• 2020

Techniques used for the regulation of gene expression facilitate studies of gene function and treatment of diseases via gene therapy. Many tools have been developed for the regulation of gene expression in mammalian cells. The Lac operon system induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) is one of the employed inducible systems. IPTG mimics the molecular structure of allolactose and has a strong affinity for the corresponding repressor. IPTG is known to rapidly penetrate into mammalian cells and exhibits low toxicity. In the present study, we developed a new inducible expression system that could regulate the expression of genes in mammalian cells using IPTG. Here we confirm that unlike other vector systems based on the Lac operon, this expression system allows regulation of gene expression with lactose in the mammalian cells upon transfection. The co-treatment with IPTG and lactose could improve the regulatory efficiency of the specific target gene expression. The regulation of gene expression with lactose has several benefits. Lactose is safe in humans as compared to other chemical substances and is easily available, making this technique very cost-effective.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 하계종합학술대회 논문집(3)
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• pp.25-28
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• 2002

This paper introduce a personalized facial expression recognition system. Many previous works on facial expression recognition system focus on the formal six universal facial expressions. However, it is very difficult to make such expressions for normal person without much effort and training. And in these days, the personalized service is also mainly focused by many researchers in various fields. Thus, we Propose a novel facial expression recognition system with fuzzy neural networks and robust image processing.

• 한국실내디자인학회논문집
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• 제26호
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• pp.64-71
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• 2001

After Industrial Revolution, new thought system is needed to subdue multi-chanties and complexities of society. Nowadays under 'system theory', these efforts are theorized and organized in fields of science, philosophy and others. From Industrial revolution, before establishing the system theory, system has been needed by the expression methods. From that time, as it could be done, standardization, has been continued. Finally these conclusion reached at the systematic expression methods and when the system was build up, this theory was come true in Art such as De Stijl, Neo-Pasticism, Russian Suprematism and Constructism. For that reason, formal solution to achieve mass reductions was found in Art, so people connected Industry with Art at that time. Systematic expression methods in Art have characteristics of system and reflect mathematics and geometry theory which is based on the system theory. This study analyzes the systematic methods in contemporary interior space through the concept of systematic expression methods in Swiss Allianz's works which used the systematic expression methods and also inquires how the common meaning of system after recent times effect to contemporary design.