• The KIPS Transactions:PartA
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• v.14A no.2
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• pp.85-90
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• 2007

This paper presents a facial animation and expression control method that enables the animator to select any facial frames from the facial expression space, whose expression transfer paths the system can setup automatically. Our system creates the facial expression space from approximately 2500 captured facial frames. To create the facial expression space, we get distance between pairs of feature points on the face and visualize the space of expressions in 2D space by using the Multidimensional scaling(MDS). To setup most suitable expression transfer paths, we classify the facial expression space into four field on the basis of any facial expression state. And the system determine the state of expression in the shortest distance from every field, then the system transfer from the state of any expression to the nearest state of expression among thats. To complete setup, our system continue transfer by find second, third, or fourth near state of expression until finish. If the animator selects any key frames from facial expression space, our system setup expression transfer paths automatically. We let animators use the system to create example animations or to control facial expression, and evaluate the system based on the results.

• Korean Institute of Interior Design Journal
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• v.14 no.6 s.53
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• pp.168-176
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• 2005

This study is a understanding on technological specific expression to reflect of a research institute in modern architecture. For this study, it separately reduce space program, plan program, design elements of elevation, structural system, mechanical system, building material program to its expressional elements of research institute. Also, it's arranged about a specificity that is expressed for technology each plan elements of a research institute. As a result, elements what is expressive of technology on research institute are influenced an aesthetic expression, expression of an arrangement system, structural expression, expression of architectural organization, and it influences more getting feels technological specificity expressed an aesthetic, structural system, architectural composition, mechanical distribution system in order of their magnitude. This is offered suggestion what must be an achievable complex both a simply technological expression and aesthetic expression.

• The Journal of the Korea Contents Association
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• v.8 no.2
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• pp.18-26
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• 2008

This paper presents a phased visualization method of facial expression space that enables the user to control facial expression of 3D avatars by select a sequence of facial frames from the facial expression space. Our system based on this method creates the 2D facial expression space from approximately 2400 facial expression frames, which is the set of neutral expression and 11 motions. The facial expression control of 3D avatars is carried out in realtime when users navigate through facial expression space. But because facial expression space can phased expression control from radical expressions to detail expressions. So this system need phased visualization method. To phased visualization the facial expression space, this paper use fuzzy clustering. In the beginning, the system creates 11 clusters from the space of 2400 facial expressions. Every time the level of phase increases, the system doubles the number of clusters. At this time, the positions of cluster center and expression of the expression space were not equal. So, we fix the shortest expression from cluster center for cluster center. We let users use the system to control phased facial expression of 3D avatar, and evaluate the system based on the results.

• Journal of Genetic Medicine
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• v.3 no.1
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• pp.33-37
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• 1999

Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expression level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.

• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.3
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• pp.255-263
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• 2006

In the last decade, face analysis, e.g. face detection, face recognition, facial expression recognition, is a very lively and expanding research field. As computer animated agents and robots bring a social dimension to human computer interaction, interest in this research field is increasing rapidly. In this paper, we introduce an artificial emotion mimic system which can recognize human facial expressions and also generate the recognized facial expression. In order to recognize human facial expression in real-time, we propose a facial expression classification method that is performed by weak classifiers obtained by using new rectangular feature types. In addition, we make the artificial facial expression using the developed robotic system based on biological observation. Finally, experimental results of facial expression recognition and generation are shown for the validity of our robotic system.

• Reproductive and Developmental Biology
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• v.37 no.3
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• pp.109-115
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• 2013

Until recently the most popular tetracycline-inducible gene expression system has been the one developed by Gossen and Bujard. In this study, we tested the latest version of same system and the results are summarized as follows: Compared with previous one, the difference of new system are minor changes of nucleotide sequences in transactivator and tetracycline response element (TRE) regions. Sensitivity to the doxycycline (a tetracycline derivative) was improved. Leakiness of GFP marker gene expression in non-inducible condition was significantly decreased. Higher expression of the marker gene was observed when the cells were fed with doxycycline-containing medium. Optimal insertion site of woodchuck posttranscriptional regulatory element (WPRE) sequence which was known to increase gene expression was different depending on the origin of cells. In chicken embryonic fibroblast, location of WPRE sequence at 3' end of TRE resulted in the highest GFP expression. In bovine embryonic fibroblasts, 3' end of transactivator was the best site for the GFP expression.

• Journal of Microbiology and Biotechnology
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• v.30 no.8
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• pp.1124-1131
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• 2020

Techniques used for the regulation of gene expression facilitate studies of gene function and treatment of diseases via gene therapy. Many tools have been developed for the regulation of gene expression in mammalian cells. The Lac operon system induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) is one of the employed inducible systems. IPTG mimics the molecular structure of allolactose and has a strong affinity for the corresponding repressor. IPTG is known to rapidly penetrate into mammalian cells and exhibits low toxicity. In the present study, we developed a new inducible expression system that could regulate the expression of genes in mammalian cells using IPTG. Here we confirm that unlike other vector systems based on the Lac operon, this expression system allows regulation of gene expression with lactose in the mammalian cells upon transfection. The co-treatment with IPTG and lactose could improve the regulatory efficiency of the specific target gene expression. The regulation of gene expression with lactose has several benefits. Lactose is safe in humans as compared to other chemical substances and is easily available, making this technique very cost-effective.

• Proceedings of the IEEK Conference
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• 2002.06c
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• pp.25-28
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• 2002

This paper introduce a personalized facial expression recognition system. Many previous works on facial expression recognition system focus on the formal six universal facial expressions. However, it is very difficult to make such expressions for normal person without much effort and training. And in these days, the personalized service is also mainly focused by many researchers in various fields. Thus, we Propose a novel facial expression recognition system with fuzzy neural networks and robust image processing.

• Korean Institute of Interior Design Journal
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• no.26
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• pp.64-71
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• 2001

After Industrial Revolution, new thought system is needed to subdue multi-chanties and complexities of society. Nowadays under 'system theory', these efforts are theorized and organized in fields of science, philosophy and others. From Industrial revolution, before establishing the system theory, system has been needed by the expression methods. From that time, as it could be done, standardization, has been continued. Finally these conclusion reached at the systematic expression methods and when the system was build up, this theory was come true in Art such as De Stijl, Neo-Pasticism, Russian Suprematism and Constructism. For that reason, formal solution to achieve mass reductions was found in Art, so people connected Industry with Art at that time. Systematic expression methods in Art have characteristics of system and reflect mathematics and geometry theory which is based on the system theory. This study analyzes the systematic methods in contemporary interior space through the concept of systematic expression methods in Swiss Allianz's works which used the systematic expression methods and also inquires how the common meaning of system after recent times effect to contemporary design.