• Communications for Statistical Applications and Methods
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• 제11권1호
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• pp.59-77
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• 2004

Since the introduction of DNA microarray, a revolutionary high through-put biological technology, a lot of papers have been published to deal with the analyses of the gene expression data from the microarray. In this paper we review most papers relevant to the cDNA microarray data, classify them in statistical methods' point of view, and present some statistical methods deserving consideration and future study.

• 대한핵의학회지
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• 제34권1호
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• pp.1-9
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• 2000

The rapid progress of molecular genetic methods over the past two decades has necessitated the development of methods to detect and quantify genetic activity within living bodies. Reporter genes provide a rapid and convenient tool to monitor gene expression by yielding a readily measurable phenotype upon expression when introduced into a biological system. Conventional reporter systems, however, are limited in their usefulness for in vivo experiments or human gene therapy because of its invasive nature which requires cell damage before assays can be performed. This offers an unique opportunity for nuclear imaging techniques to develope a novel method for imaging both the location and amount of gene expression noninvasively. Current developments to achieve this goal rely on utilizing either reporter enzymes that accumulate radiolabeled substrates or reporter receptors that bind specific radioligands. This overview includes a brief introduction to the background for such research, a summary of published results, and an outlook for future directions.

• 한국콘텐츠학회논문지
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• 제10권7호
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• pp.198-207
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• 2010

그림책화면에서 나타나는 시각적 의미형성은 전달하고자 하는 현상이나 메시지를 직접적으로 보여주는 것뿐만 아니라 다른 이미지를 더하거나 과장, 축소하여 대상의 속성이나 특성을 보다 쉽고 빠르게 이해시켜줄 수 있다는 점에서 독창적인 표현방법이 된다. 본 연구는 어린이그림책에서 나타나는 은유적 표현방식이 어린이에게 시각적 심리적으로 어떻게 작용하는지 분석하기 위하여 먼저, 시각적 은유특성과 표현형식에 대한 이론적 고찰을 통해 그림책일러스트레이션의 표현방법에 대한 범주화의 틀을 마련하였다. 또한 실증조사를 통해 어린이의 시각적 심리적 지각반응을 명확하게 도출하기 위하여 표현형식을 2가지 유형으로 구분하고, 이를 심층면접의 설문자료로 활용하여 유형별 어린이의 언어적 심리적 반응을 분석하였다. 끝으로 본 연구가 제시한 결론은 일러스트레이터의 개성과 창의성이 바탕이 된 화면제작에 도움이 됨은 물론, 일러스트레이션을 배우는 학생들에게 효과적인 표현방법연구와 실험에 활용될 것으로 사료된다.

• 패션비즈니스
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• 제27권6호
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• pp.66-84
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• 2023

Handbags are a part of fashion and while their significance and value are increasing, research on this topic is lacking. This study defines handbags and categorizes the materials used for making handbags, sewing methods, expression techniques, and terminologies related to accessories. A total of 1,743 handbags that were released from the Spring 2020 to Fall 2023, Ready-to-Wear collections by 8 selected brands (Hermes, Dior, Fendi, Chanel, Louis Vuitton, Prada, Gucci, and Alexander McQueen), were analyzed. Out of these, 732 unique designs, excluding those with only color variations, were studied. The most common sewing methods were 'Cut, sewing, and edge painting', 'Cylinder arm sewing', 'Cut, edge painting, and sewing', and 'Inverted seam', in that order. Slim strap designs primarily used the 'Cut, sewing, and edge painting' method, whereas the body, especially with narrow and hard leather, was best suited for the 'Cylinder arm sewing machine'. For expression techniques, the most frequently used methods were 'Quilting', 'Metal Eyelet', 'Embossing', 'Printing', 'Punching', and 'Weaving', respectively. The characteristics of each brand's production methods, expression techniques, and accessories were as follows: First, the exposure of logos and monograms is prominent. Unlike clothing, handbags often prominently feature the brand's logo or monogram. Second, signature quilting is a prominent feature. Quilting effectively conveys the brand's signature style, providing cushioning, volume, and pattern effects. Third, sustainable development is a growing trend. Brands are increasingly applying eco-friendly and socially responsible designs.

• Tuberculosis and Respiratory Diseases
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• 제83권1호
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• pp.51-60
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• 2020

Background: Programmed death-ligand 1 (PD-L1) expression is tested by immunohistochemistry (IHC)-22C3, SP263, and SP142. The aim of this study is to evaluate the correlation among the three methods of PD-L1 IHC in non-small cell lung cancer (NSCLC) and clinical significance of PD-L1 expression in lung adenocarcinoma with an epidermal growth factor receptor (EGFR)-tyrosine kinase domain mutation. Methods: The results of 230 patients who were pathologically confirmed as having NSCLC; tested using PD-L1 IHC 22C3, SP263, and SP142 methods; and evaluated via the peptide nucleic acid clamping method to confirm EGFR mutation, were analyzed in this study. Results: 164 patients underwent both the SP263 and 22C3 tests. There was a significant positive correlation between the outcomes of the two tests (Spearman correlation coefficient=0.912, p<0.001), with a derived regression equation as follows: 22C3=15.2+0.884×SP263 (R²=0.792, p<0.001). There was no relationship between the expression of PD-L1 and clinical parameters, including EGFR-tyrosine kinase inhibitor (TKI) mutation. The PD-L1 expression in patients treated with EGFR-TKI yielded a 2-month-shorter progression period than that in the PD-L1-negative group. However, this did not reach statistical significance (PD-L1<1% vs. PD-L1≥1%, 10 months vs. 8 months). Conclusion: The results of the 22C3 and those of SP263 methods were in good correlation with one another. Since the PD-L1 expression is not influenced by the EGFR mutation, it is necessary to perform a PD-L1 test to set the treatment direction in the patients with EGFR-mutant NSCLC.

• 한국실내디자인학회논문집
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• 제19권4호
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• pp.58-65
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• 2010

The purpose of this study is to be applied with the basic data for the design method in the housing space which is differential. So, we analyzed the expression method and the application characteristic to materials of units in housing complex. To that, we researched on the condition of finishing material about the kind of material, color, texture and pattern for components in living room space. The results of this study are as follows : First, at the present time, the Object concept is expressed by 'mixing' and 'juxtaposition' of material, texture and pattern of finishing materials in housing space. But, we could find that the Object concept was used restrictively in housing space. So, we have to find different expression methods to be the effective and differential design by being applied Object concept for the housing space. Second, the 'mixing' which is the method to express the Object concept is showed in only 'art wall'. so, we can estimate that the method is the limited method to plan the varied housing. It is needed various expression methods to design components for the housing space. Third, it showed that the 'mixing' was a passive expression method using a little differential for design of housing space. We could estimate that similar methods were applied to the housing design. By results of this study, we can think that the develop study is needed to find design methods for expression of the Object in housing space.

• 한국의류산업학회지
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• 제11권1호
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• pp.24-32
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• 2009

The purpose of this study is to understand characteristic of expression in Korean style fashion design and investigated Korean style fashion design's deployment and design idea method as well as its esthetic value. The photographs for the research were selected from fashion collections during S/S 2003-F/W 2007 by four specialists in the department of textile and then analyzed with 288 final data. The results were as followings. First, the characteristics of design expression in Korean style fashion design were formation by separating partial shape from the whole, formation by shape's separation and repetition, and connecting formation by combination and transformation of parts. In the case of color, they were direct expression, contrasting formative expression, and gradual changing expression. In the case of pattern, they were the methods of filling, filling & emptying, and partial filling. Second, the deployments of Korean style fashion design were adding modern elements to traditional things, combining traditional elements and modern things, and adding traditional elements to modern things. The third, Korean style fashion design's idea methods were weaving, snatching, adding, and changing. Fourth, it was shown that esthetic values of Korean style fashion design were the interactive organism by building organic relationship, the optimum expressing beauty with minimum elements, the palpitation having dynamics and rhythmical beauty.

• 패션비즈니스
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• 제11권3호
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• pp.133-151
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• 2007

The role of fabric now directly related with the expression of the beauty of clothing and it provides new and creative ideas. This study was aimed at reviewing basic data that can be used in systematic design development through fabric expression for today's fashion designers who must study unique, original fashion design development. For systematic development of design technique through fabric expression, fabric expression methods and characteristics, aesthetic characteristics and fabric design of 'art to wear' were reviewed and the results are as follows. First, the highly wrought fabric expression of art to wear was confirmed to be comprehending a message within itself. Second, aesthetic characteristics of fabric expression used in art to wear can be classified as decorativeness, extensity, 2-D pictorialness, handicraft, compounding and rearrangement, and 3-D characteristics. Third, the 6 aesthetic characteristics have unique design features and aesthetic categories. The understanding the fabric expression techniques through study on the classification of the fabric expression in 'art to wear' is expected to be extended to proposition of creative direction and inspiration of modern fashion.

• Asian Pacific Journal of Cancer Prevention
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• 제14권7호
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• pp.4209-4214
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• 2013

Background: Breast cancer is among the five most common cancers and ranks first among cancers diagnosed in Iranian women. Screening and treatment of this disease with molecular methods, especially regarding high incidences at early age and advanced stage, is essential. Several genes with altered expression have been identified by cDNA microarray studies in breast cancer, with the Bcl-2 gene indicated as a likely candidate. In this study, we studied Bcl-2 gene expression levels in parallel tumor and non-tumor breast tissues. Materials and Methods: Forty samples including 21 tumor, 16 non tumor (marginal) and 3 benign breast tissues which were all pathologically diagnosed, were subjected to RNA extraction and polyA RT-PCR with the expression level of Bcl-2 quantified using real-time PCR. Results: There is higher expression levels of the Bcl-2 gene in tumor samples compared with marginal samples, but not attaining significance(p>0.05). Bcl-2 expression in 14 (66.7%) of the cases of tumor samples and 9 (56.3%) cases of the marginal samples were positive. Comparison of the expression of the Bcl-2 gene in histological grade showed that a high expression of Bcl-2 was associated with a high histological grade (p<0.41). Conclusions: Our data suggests that dysregulated Bcl-2 gene expression is potentially involved in the pathogenesis of breast cancer. Using gene expression analysis may significantly improve our ability for screening cancer patients and will prove a powerful tool in the diagnosis and prognostic evaluation of the disease whilst aiding the cooperative group trials in the Bcl-2 based therapy project.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.23-30
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• 2019

Objective: Recent studies have demonstrated that lin-28 homolog B (LIN28B)/miRNA let-7 (let-7) plays a role in the regulation of pubertal onset in mammals. However, the role of LIN28B/let-7 in the onset of ovine puberty remains unknown. We cloned the Duolang sheep Lin28B cDNA sequence, detected the expression change of LIN28B, let-7a and let-7g in hypothalamus, pituitary and ovary tissues at three different pubertal stages. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of LIN28B gene from Duolang sheep and the bioinformatics methods were applied to analyze the amino acid sequence of LIN28B protein. The mRNA expression levels of the LIN28B gene at different pubertal stages were examined by real time RT-PCR. Results: LIN28B cDNA of Duolang sheep was cloned, and two transcripts were obtained. The amino acid sequence of transcript 1 shares 99.60%, 98.78%, and 94.80% identity with those of goat, wild yak and pig, respectively. Strong LIN28B mRNA expression was detected in the hypothalamus, pituitary, ovary, oviduct and uterus, while moderate expression was found in the liver, kidney, spleen and heart, weak expression was observed in the heart. No expression was found in the lungs. Quantitative real-time PCR (QPCR) and western-blot analysis revealed that the LIN28B was highly expressed in the hypothalamus and ovary at prepuberty stages, and this expression significantly decreased from the prepuberty to puberty stages (p<0.05). Markedly increased levels of mRNA expression were detected in the pituitary from prepuberty to puberty (p<0.05) and then significantly decreased from puberty to post-puberty (p<0.05). The expression levels of let-7a and let-7g showed no significant changes among different pubertal stages (p>0.05). Conclusion: These results provided a foundation for determining the functions of LIN28B/let-7 and their role in the onset of sheep puberty.