• Proceedings of the Korean Society for Bioinformatics Conference
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• 2000.11a
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• pp.59-60
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• 2000

The past few years have seen a dramatic increase in gene expression data on the basis of DNA microarrays or DNA chips. Going beyond a generic view on the genome, microarray data are able to distinguish between gene populations in different tissues of the same organism and in different states of cells belonging to the same tissue. This affords a cell-wide view of the metabolic and regulatory processes under different conditions, building an effective basis for new diagnoses and therapies of diseases. In this talk we present machine learning techniques for effective mining of DNA microarray data. A brief introduction to the research field of machine learning from the computer science and artificial intelligence point of view is followed by a review of recently-developed learning algorithms applied to the analysis of DNA chip gene expression data. Emphasis is put on graphical models, such as Bayesian networks, latent variable models, and generative topographic mapping. Finally, we report on our own results of applying these learning methods to two important problems: the identification of cell cycle-regulated genes and the discovery of cancer classes by gene expression monitoring. The data sets are provided by the competition CAMDA-2000, the Critical Assessment of Techniques for Microarray Data Mining.

• Journal of Microbiology
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• v.34 no.1
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• pp.15-22
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• 1996

The cloned X-bacterial gene (groEx) which is analogous to groE of E. Coli strongly expressed in E. coli when grown at the temperature 27.deg. C or higher without having to add any inducers. By S1-nuclease mapping, primer extension analysis and site directed mutagenesis, we found 4 promoters in the gene. Among them two promoters located at 5'-extended region of the gene are homologous to the promoters found in groE family of heat-shock genes ; they are , .sigma.$^{32}$ factor-dependent P1 promotor and .delta$^{70}$factor-dependet P2 promoter. The other two promoters found within the coding region of groESx were P3, 5'-TTGGCG-(18 bases)-AATACT-3' and P4, 5'-TTGGCA-(19 bases)-TAAGT which overlapped within 49 bases. These unique intragenic .delta.$^{70}$-dependent promoters are the first to be cloned and characterized in groE analogous heat-shock genes so far. These P3 and P4 promoters appeared to be responsible for the strong expression of GroElx in X-bacteria in vivo.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1473-1477
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• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.151-162
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• 1996

Pseudorabies is caused by Pseudorabies virus (PRV: Aujeszky's disease virus) of Herpesviridae that is characterized by 100 to 150nm in size with a linear double-stranded DNA molecule with of approximately $90{\times}10^6Da$. This disease affects most of domestic animals such as swine, cattle, dog, sheep, cat, chicken, etc. causing high mortality and economic losses. In swine, young piglets show high mortality and pregnant sows, reproductive failures. However the adult swine reveals no clinical signs in general. But they become a carrier state and play an important role for propagation of the disease. In this study, the nucleotide sequence of major casid protein gene of PRV, Yangsan strain isolated from the diseased swine in Korea was analyzed, and the recombinant MCP was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. As result, in BamHI digestion, MCP gene locus of PRV YS strain showed different from that of Indiana S strain. The patterns of enzyme mapping were also found to be unidentical each other. The sequence of the MCP gene partially analyzed showed 98.09% identity to Indiana S strain. The expression of MCP in Sf-9 cell cotransfected by pVLMCP-44 baculovirus expression vector was characterized by Southern blot hybridization, immunofluoresent and immunocytochemical tests, SDS-PAGE and Western blotting. The rMCP with M.W. 142kDa was most effectively expressed in Sf-9 cells at the 3-4th days post inoculation of the recombinant baculovirus by 2 moi.

• The Research Journal of the Costume Culture
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• v.13 no.4 s.57
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• pp.550-563
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• 2005

These days, photoshop and illustrator program can make a fashion illustration express easily and speedily, And they can also express a feeling and sensitivity of fashion illustrator by a tool and effect more than a manual work's media. The purpose of this study was to analyze a visual expression and characteristics in fashion illustration using 2D graphics. The method of this study was to analyze the fashion illustration books using 2D graphics. The visual expressions in fashion illustration using 2D graphics were as follows: Fashion illustration was based on a sketch or photography, and used a composition, drawing, mapping, painting, and manual work's re-touching. Characteristics of visual expression were as follows: First, a image composition was showed discontinuity and heterogeneity of image, new context and composition, and allowance of reality. Second, image transform was showed image overlap, body transformation by image recomposition, and deformed transformation by vector drawing. Third, hyper-real was showed precise touching, mechanical and neutral image, omission of background and focus of an object's characteristic and information. Fourth, following a realistic expression was showed simplified color, shading, dominant view point of fashion illustrator by omission, and daily lift style. Fifth, following a pictorial expression was showed non-fixed and irregular line, natural painting, and drawing and painting by conventional pictorial media. In conclusion, a photoshop and illustrator in 2D graphics will serve as a new media far fashion illustration with a manual work. And they will not only intensify a capacity as a commercial role of fashion illustration but also present a positive motive for students learning a fashion illustration.

• Journal of Microbiology
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• v.42 no.4
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• pp.361-364
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• 2004

Internal ribosome entry site (IRES) of the hepatitis C virus (HCV) is known to be essential for HCV replication and most conserved among HCV variants. Hence, IRES RNA is a good therapeutic target for RNA-based inhibitors, such as ribozymes. We previously proposed a new anti-HCV modulation strategy based on trans-splicing ribozymes, which can selectively replace HCV transcripts with a new RNA that exerts anti-HCV activity. To explore this procedure, sites which are accessible to ribozymes in HCV IRES were previously determined by employing an RNA mapping method in vitro. In this study, we evaluate the intracellular accessibility of the ribozymes by comparing the trans-splicing activ­ities in cells of several ribozymes targeting different sites of the HCV IRES RNA. We assessed the intra­cellular activities of the ribozymes by monitoring their target-specific induction degree of both reporter gene activity and cytotoxin expression. The ribozyme capable of targeting the most accessible site iden­tified by the mapping studies then harbored the most active trans-splicing activity in cells. These results suggest that the target sites predicted to be accessible are truly the most accessible in the cells, and thus, could be applied to the development of various RNA-based anti-HCV therapies.

• Journal of The Korean Society of Agricultural Engineers
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• v.50 no.3
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• pp.115-123
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• 2008

Evapotranspiration mapping using both meteorological ground-based measurements and satellite-derived information has been widely studied during the last few decades and various methods have been developed for this purpose. It is significant and necessary to estimate regional evapotranspiration (ET) distribution in the hydrology and water resource research. The study focused on analyzing the surface ET of Chungbuk region using Landsat 7 ETM imagery. For this process, we estimated the regional daily evapotranspiration on May 8, 2000. The estimation of surface evapotranspiration is based on the relationship between Temperature Vegetation Dryness Index (TVDI) and Morton's actual ET. TVDI is the relational expression between Normalized Difference of Vegetation Index (NDVI) and Land Surface Temperature (LST). The distribution of NDVI corresponds well with that of land-use/land cover in Chungbuk. The LST of several part of city in Chungbuk region is higher in comparison with the averaged LST. And TVDI corresponds too well with that of land cover/land use in Chungbuk region. The low evapotranspiration availability is distinguished over the large city like Cheongju-si, Chungju-si and the difference of evapotranspiration availability on forest and paddy is high.

• Geomechanics and Engineering
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• v.11 no.3
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• pp.325-343
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• 2016

A total stress-based bounding surface model is developed to predict the undrained behaviour of saturated soft clays under cyclic loads based on the anisotropic hardening modulus field and bounding-surface theories. A new hardening rule is developed based on a new interpolation function of the hardening modulus that has simple mathematic expression and fewer model parameters. The evolution of hardening modulus field is described in the deviatoric stress space. It is assumed that the stress reverse points are the mapping centre points and the mapping centre moves with the variation of loading and unloading paths to describe the cyclic stress-strain hysteresis curve. In addition, by introducing a model parameter that reflects the accumulation rate and level of shear strain to the interpolation function, the cyclic shakedown and failure behaviour of soil elements with different combinations of initial and cyclic stresses can be captured. The methods to determine the model parameters using cyclic triaxial compression tests are also studied. Finally, the cyclic triaxial extension and torsional shear tests are performed. By comparing the predictions with the test results, the model can be used to describe undrained cyclic stress-strain responses of elements with different stress states for the tested clays.

• Journal of the Korean Home Economics Association
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• v.40 no.6
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• pp.159-171
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• 2002

Today, many people seek for their own personal character which is distinguished from another people and they utilize fashion coordination as the was of expression their own image. In addition, interest in electronic commerce and cuber shopping mall on the internet is increasing. For this reason, visual and interesting virtual fashion coordination system is needed. The purpose of this study is to propose possibility of fashion coordination by virtual 3D model. For this study, 1. We make a 3D standard body model by automatic generation. 2. We make 3D fashion item (sleeveless top and flare skirt) by automatic generation. 3. We combine 3D body model with fashion item by special point, grouping and gap being between body and clothes. 4. We make textile palettes and textile DB for texture mapping and rendering. As a effect of this study, 1. It can give the chance to coordinate clothes suitable for their own character and bodyshape on the cuber space more speedily and variously. 2. It can help fashion internet shopping mall company can save a time, expenses and tries to advertise their new products, offer service for customers and lead customers to purchasing. 3. It can accumulate a database of design and textile for using by fashion and textile industry.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.224-231
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• 1993

The nucleotide sequence of the xylanase gene (xynY) from alkalophilic Bacillus sp. YC-335 was determined and analyzed. An open reading frame of 1, 062 base pairs for xynY gene was observed and encoded for a protein of 354 amino acids with a molecular weight of 38, 915. S1 nuclease mapping showed that the transcription initiation sites of the xynY gene were different in Bacillus sp. YC-335 and Escherichia coli HB101 (pYS55). S1 mapping also showed that -10 region of the xynY gene recognized by RNA polymerases of E. coli and Bacillus sp. YC-335 were TACAGT and TATGAT , respectively. A ribosome binding site sequence with the free energy of -17.0 Kcal/mol was observed 9 base pairs upstream from the unusual initiation codon, TTG. The proposed signal sequence consisted of 27 amino acids, 2 of which were basic amino acid residues and 21 were hydrophobic amino acid residues. When the amino acid sequences of xylanases were compared, Bacillus sp. YC-335 xylanase showed more than 50% homology with xylanases from B. pumilus, B. subtilis, and B. circulans.