• IMMUNE NETWORK
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• 제23권3호
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• pp.23.1-23.17
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• 2023

Inflammation is a series of host defense processes in response to microbial infection and tissue injury. Inflammatory processes frequently cause extracellular acidification in the inflamed region through increased glycolysis and lactate secretion. Therefore, the immune cells infiltrating the inflamed region encounter an acidic microenvironment. Extracellular acidosis can modulate the innate immune response of macrophages; however, its role for inflammasome signaling still remains elusive. In the present study, we demonstrated that macrophages exposed to an acidic microenvironment exhibited enhanced caspase-1 processing and IL-1β secretion compared with those under physiological pH. Moreover, exposure to an acidic pH increased the ability of macrophages to assemble the NLR family pyrin domain containing 3 (NLRP3) inflammasome in response to an NLRP3 agonist. This acidosis-mediated augmentation of NLRP3 inflammasome activation occurred in bone marrow-derived macrophages but not in bone marrow-derived neutrophils. Notably, exposure to an acidic environment caused a reduction in the intracellular pH of macrophages but not neutrophils. Concordantly, macrophages, but not neutrophils, exhibited NLRP3 agonist-mediated translocation of chloride intracellular channel protein 1 (CLIC1) into their plasma membranes under an acidic microenvironment. Collectively, our results demonstrate that extracellular acidosis during inflammation can increase the sensitivity of NLRP3 inflammasome formation and activation in a CLIC1-dependent manner. Thus, CLIC1 may be a potential therapeutic target for NLRP3 inflammasome-mediated pathological conditions.

• 대한치과보철학회지
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• 제50권4호
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• pp.249-257
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• 2012

연구 목적: 본 연구는 광주지역 주민의 나이에 따른 미소형태를 분석하고 설문조사를 통해 심미적인 미소형태를 분석하여 전치부의 보철 수복에 도움을 주고자 하였다. 연구 재료 및 방법: 20대(평균연령 27.2세)와 50, 60대(평균연령 55.6세)의 정상 교합자를 각각 33명(남자 15명, 여자 18명), 20명 (남자 7명, 여자 13명)을 대상으로 하였고, 안정위 시와 미소 시 얼굴정면 사진을 촬영하여 미소 시 입술 형태, 입술의 변화와 치아와의 관계를 계측, 분석하였다. 그 후, 전남대학교 치의학전문대학원 1학년(67명), 4학년(47명)을 대상으로 아름다운 미소에 대한 설문조사를 실시하였다. 결과: 미소 시 상순과 치아와의 관계는 두 그룹 모두 average smile이 가장 많았으며, smile line ratio는 청년층에서 1.09, 장년층에서 0.90으로 나타나 거의 평행한 관계를 보였다. 미소 시 buccal corridor ratio가 커질수록 노출되는 치아 수는 적어지는 역상관관계를 보였다. 미소 시 노출되는 치아는 청년층에서 제2소구치까지 보이는 경우가 63.64%로 제일 많았고, 장년층에서는 제1소구치까지 보이는 경우가 35%로 가장 많았다. 미소 시 상순 하연의 수직적 길이 변화와 상악 중절치 노출 임상치관 길이 사이에는 서로 역상관관계가 보였으며, 하순 상연의 길이 변화와 상악 중절치 노출 임상치관 길이 사이에는 아무런 상관관계가 나타나지 않았다. 결론: 심미성에 관하여는 High smile line과, 미소 시 상악 제1소구치까지 보이는 경우가 제일 심미성이 높았으며, 전남대학교 치의학전문대학원 1학년의 경우, 얼굴전체 사진과 입술만 나타난 사진에서 뽑은 아름다운 미소 사진이 달랐으며, 4학년의 경우에는 일치하였다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.966-970
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• 2011

The thermo-mechanical interaction between brake block and wheel tread during braking has been found to cause thermal crack on the wheel tread. Due to thermal expansion of the rim material, the thermal cracks will protrude from the wheel tread and be more exposed to wear during the wheel/block contact than the rest of the tread surface. The wheel rim is in residual compression stress when is new. After service running, the region in the tread has reversed to tension. This condition can lead to the formation and growth of thermal cracks in the rim which can ultimately lead to premature failure of wheel. In the present paper, the thermal cracks of railway wheel, one of severe damages on the wheel tread, were evaluated to understand the safety of railway wheel in running condition. The residual stresses for damaged wheel which are applied to tread brake are investigated. Mainly X-ray diffusion method is used. Under the condition of concurrent loading of continuous rolling contact with rails and cyclic frictional heat from brake blocks, the reduction of residual stress is found to correlate well with the thermal crack initiation.

• 대한전자공학회논문지SP
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• 제47권3호
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• pp.70-80
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• 2010

본 논문에서는 치과용 의료시스템(Dental PACS)에서 필요한 시계열 분석(Subtraction Radiography) 방법에 기반 하여 기존의 연구에서 갖지 못했던 영상정렬의 자동화 방안과 미세변화 검출 방안을 제안한다. 제안된 방법은 기존의 단일 템플릿 방식의 문제점을 해결하기 위해 인공치아 및 자연치아의 기하학적 형태 정보를 이용하여 각 치아에 대한 관심영역을 획득하고 획득된 관심영역을 기준으로 지식 기반(knowlege-based)의 다중 템플릿(multi-template) 방식의 정합을 통해 치아 주위에서 발생되는 미세변화를 빠르고 정확하게 검출할 수 있다. 실험결과 제안된 방법은 신속하고 정확하게 치아 주위의 미세변화에 대한 검출이 가능하였고, 보다 객관적이고 정량적인 결과를 제시할 수 있음을 보여주었다.

• Current Photovoltaic Research
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• 제6권2호
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• pp.39-42
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• 2018

When the PV module is illuminated in a high temperature region, solar cells are also exposed to the high temperature external environment. The operating temperature of the solar cell inside the module is increased, which causes the power drops. Various efforts have been made to reduce the operating temperature and compensate the power of solar cells according to the outdoor temperature such as installing of a cooling system. Researches have been also reported to lower the operating temperature of solar cells by improving the heat dissipation properties of the backsheet. In this study, we conducted a test to measure the internal temperature of each module components and the external temperature when the light was irradiated according to the surrounding temperature. Backsheets with different thermal conductivities were compared in the test. Finally, in order to explain the temperature difference between the solar cell and the outside of the module, we proposed an evaluation method of the heat transfer characteristics of photovoltaic modules with different backsheet.

• 열처리공학회지
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• 제33권3호
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• pp.129-134
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• 2020

Due to excellent properties such as high specific strength and low density, application of magnesium alloys have been rapidly increased. However, magnesium alloy has a serious problem that is easily oxidized when exposed to high-temperature. For this reason, magnesium alloys have been generally used for SF6 gas such as protective cover gas in casting and melting, but it has been reported that this gas has a serious influence on global warming. Therefore, many researchers have been studied to improve the oxidation resistance of magnesium alloy. It was reported that addition of Be, Ca and CaO in magnesium alloy can improve the oxidation properties. In this study, the possibility of improving the oxidation resistance by adding CaO extracted from oyster shells was investigated. Oyster shells were completely decomposed into CaO and CO₂ by annealing. With the addition of CaO, a coexistence region of MgO + CaO was formed in the oxide layer and its thickness was also reduced.

• 대한의생명과학회지
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• 제18권2호
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• pp.160-164
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• 2012

The baculovirus/insect cell expression system is of great value for the large-scale production of normal and mutant mammalian passive glucose-transport proteins heterologously for structural and functional studies. In most mammalian cells that express HepG2, this transporter isoform is predominantly located at the cell surface. However, it had been reported that heterologous expression of other membrane proteins using the baculovirus system induced highly vacuolated cytoplasmic membranes. Therefore, how a cell responds to the synthesis of large amounts of a glycoprotein could be an interesting area for investigation. In order to examine the subcellular location of the human HepG2 transport proteins when expressed in insect cells, immunofluorescence studies were carried out. Insect cells were infected with the recombinant baculovirus AcNPVHIS-GT or with wild-type virus at a MOI of 5, or were not exposed to viral infection. A high level of fluorescence displayed in cells infected with the recombinant virus indicated that transporters are expressed abundantly and present on the surface of infected Sf21 cells. The evidence for the specificity of the immunostaining was strengthened by the negative results shown in the negative controls. Distribution of the transporter protein expressed in insect cells was further revealed by making a series of optical sections through an AcNPVHIS-GT-infected cell using a confocal microscope, which permits optical sectioning of cell sample. These sections displayed intense cytoplasmic immunofluorecence surrounding the region occupied by the enlarged nucleus, indicating that the expressed protein was present not only at the cell surface but also throughout the cytoplasmic membranous structures.

• Journal of Trauma and Injury
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• 제33권1호
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• pp.48-52
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• 2020

Composite skull defects in patients with severe head injuries are very challenging to manage. The dilemma when deciding whether to perform a definitive reconstruction is how long to wait for physiological recovery before an intervention complicates the situation. The inability of such patients to tolerate prolonged anesthetic exposure is a driving factor for performing the minimal intervention necessary to facilitate recovery. Herein, we present a case involving the successful immediate reconstructive treatment of a severely head-injured adolescent with a composite scalp defect secondary to trauma. A 14-year-old boy sustained a severe head injury from a motor vehicle accident with a composite scalp defect in the right fronto-parietal region. The frontal lobe was exposed, and the right eye was crushed and devitalized. The patient was deeply unconscious for 3 days, without any significant improvements before reconstructive surgery was proposed due to fear of possible meningitis resulting from the exposure of brain structures. We successfully managed the patient with a fronto-parieto-occipital flap, after which the patient promptly recovered consciousness.

• 한국지역사회생활과학회지
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• 제18권1호
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• pp.87-95
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• 2007

This study was to determine the effect of cooling parts of the limbs without harm to health. The results provide basic data for the development of clothing which could increase work efficiency and reduce body strain in hot environments. Five male adults took part in the study, conducted in a climate chamber with an ambient temperature of $37^{\circ}C$ and a relative humidity of 50%. The limbs were divided into six areas to be cooled: upper arm, forearm, thigh, calf, hand, and foot. According to preceding studies, permissible cooling safety limits of skin temperature for each part of the body for one-hour were $20^{\circ}C$ on the upper arm, forearm, thigh, and calf, and $23^{\circ}C$ on the hand and foot. For this reason, cooling the skin of each region was carried out at the above mentioned temperatures. In conclusion, cooling the hand and foot reduced perspiration, rectal temperature and heart rate. Therefore, the heat stress of workers exposed to hot environments would be reduced by decreased subjective sensations of heat and increased comfort. The effectiveness of cooling was better on the arm than on the leg.

• 한국초지조사료학회지
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• 제38권3호
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• pp.170-174
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• 2018

Environmental stresses caused by climate change, such as high temperature, drought and salinity severely impact plant growth and productivity. Among these factors, high temperature stress will become more severe during summer. In this study, we examined physiological and molecular responses of maize plants to high temperature stress during summer. Highest level of $H_2O_2$ was observed in maize leaves collected July 26 compared with June 25 and July 12. Results indicated that high temperature stress triggers production of reactive oxygen species (ROS) in maize leaves. In addition, photosynthetic efficiency (Fv/Fm) sharply decreased in leaves with increasing air temperatures during the day in the field. RT-PCR analysis of maize plants exposed to high temperatures of during the day in field revealed increased accumulation of mitochondrial and chloroplastic small heat shock protein (HSP) transcripts. Results demonstrate that Fv/Fm values and organelle-localized small HSP gene could be used as physiological and molecular indicators of plants impacted by environmental stresses.