• Journal of Animal Reproduction and Biotechnology
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• v.39 no.2
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• pp.88-94
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• 2024

Background: Using cryovial for freezing dog spermatozoa provides a practical method to increase extended sperm volume and shorten the time required for equilibration by using a simple freezing techniques. The purpose of this study was to determine the optimal thawing condition for dog sperm cryopreservation using cryovials. Methods: For sperm freezing, cryovials with 200 × 10⁶ sperm/mL were cooled after the addition of tris egg yolk extender (TEY) at 4℃ for 20 min, then TEY with 4% glycerol was added and equilibrated for another 20 min before being aligned over LN₂ vapor for another 20 min and plunged directly into LN₂. Spermatozoa were thawed in a water bath at 37℃ for varying times (25 sec, 60 sec, 90 sec, and 120 sec) in the first experiment. In the second experiment, spermatozoa were thawed in a water bath at various temperatures and times (37℃ for 1 min, 37℃ for 1 min with gentle stirring, 24℃ for 24 min, and 75℃ for 20 sec). In these experiments, the effect of thawing conditions on motility parameters, viability (SYBR-14/PI), and acrosome integrity (PSA/FITC) of spermatozoa were investigated. Results: The post-thaw sperm motility parameters, viability, and acrosome integrity were not significantly different across the experimental groups. Conclusions: In this study, the characteristics of spermatozoa frozen using cryovials were not significantly affected by various thawing conditions.

• Journal of Periodontal and Implant Science
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• v.54 no.3
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• pp.177-188
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• 2024

Purpose: Placing dental implants in areas with low bone density or in conditions where bone healing is suppressed is challenging for clinicians. An experiment using a rodent model was performed with the aim of determining the efficacy of host modulation by increasing the systemic level of cholesterol sulfate (CS) using Irosustat in the context of the bone healing process around dental implants. Methods: In 16 ovariectomised female Sprague-Dawley rats, 2 implant fixtures were placed in the tibial bones (1 fixture on each side). At 1 week after surgery, the high-CS group (n=8) received Irosustat-mixed feed, while the control group (n=8) was fed conventionally. Block specimens were obtained at 5 weeks post-surgery for histologic analysis and the data were evaluated statistically (P<0.05). Results: Unlike the high-CS group, half of the specimens in the control group demonstrated severe bone resorption along with a periosteal reaction in the cortex. The mean percentages of bone-to-implant contact (21.5%) and bone density (28.1%) near the implant surface were significantly higher in the high-CS group than in the control group (P<0.05), as was the number of Haversian canals (by 5.3). Conclusions: Host modulation by increasing the CS level may enhance the osseointegration of dental implants placed under conditions of impaired bone healing.

• Korean Journal of Cognitive Science
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• v.13 no.3
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• pp.47-60
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• 2002

The study investigated the effects of types of presentation and cognitive load on multimedia learning. In experiment 1, subject were 90 elementary school students. The subject were assigned in three conditions: Narration and Text (NT) condition, Animation and Narration(AN) condition, Animation and Text(AT) condition. The result showed that AN condition improved the learning performances in comparison with AT condition, NT condition. Experiment 2 was administrated to 87 undergraduate students. They were participated in three conditions, also. The conditions were Animation and Text (AT) condition, Animation and Narration (AN) condition, Animation, Narration and Text (ANT) condition. the results showed that AN condition was greater in AT, ANT condition. The results from a series of these experiments imply that varying the types of presentation of identical learning materials had influences on the performances. Multimedia presentation(animation and verbal conditions) improved the learning performances in comparison with monomedia presentation(verbal condition), and the advantage was raised when learners were provided the learning material in the multimodal and multimedia environment(AN condition). Also, it came out that redundant text identical to narration disrupted learning when learners were in the picture (either animation or illustration) and narration conditions. Likewise, also for adults, performances were improved in the multimodal conditions and redundant text identical to narration was not helpful for learning. These results are evidences for the dual-coding theory and the cognitive load theory.

• Journal of The Korean Association For Science Education
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• v.27 no.1
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• pp.59-69
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• 2007

The aim of this study was to investigate teachers' perceptions on MBL (microcomputer-based laboratory) experiment training program for teachers, the expecting effects of MBL experiment and application of MBL experiment after conducting MBL experiment training for science classes in schools. This study showed that most of the teachers who participated in the training program thought that the MBL experiment training program was very useful and instructive. Many teachers considered that MBL experiments using a computer could decrease time spent in the experiment by accurate and fast data collection and analysis. They also thought that the reduced time could be used more effectively in the analysis of experimental data and discussion activities leading to correct concept formation as well as in the development of graphical analysis and science process skills. However, they thought that MBL experiments were ineffective in learning how to operate experiment apparatus. This study also revealed that most teachers intended to apply MBL experiments in real classrooms context right after the training course and they pointed out many obstacles in introducing MBL experiments into their classrooms such as a budget to purchase equipment, poor laboratory conditions, and few MBL experiment training opportunities. In order to apply MBL experiment into the real classrooms, further changes were suggested as follows; development of technologies to reduce unit cost of equipment for MBL experiments, production and supply of many kinds of sensors, development of MBL experiment materials, and expansion of the training program for teachers.

• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.194-200
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• 2000

The main objective of this study was to characterize physico-chemical properties, sensory property and oxidative stability of safflower seed obtained by various roasting temperature and time. The contents of water soluble solids decreased in the higher roasting temperature and time. Sensory evaluation of safflower seed roasted in various conditions showed significant differences in taste, color, flavor and palatability. The safflower seed roasted at $190^{\circ}C$ for 20min had the best palatability. At the change of Hunter's values, L values were decreased, and a, b and ${\Delta}E$ values were increased in the higher roasting temperature and time. The content of free sugars such as sucrose and raffinose were reduced significantly in higher roasting time of $190^{\circ}C$ and $210^{\circ}C$. During the storage period after roasting treatment, peroxide values (POV) were highly increased after eight months at the all treatment except for $150^{\circ}C$. Therefore, it is inadequate over eight months after roasting treatment.

• Journal of Environmental Science International
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• v.25 no.9
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• pp.1233-1239
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• 2016

This study aimed to evaluate changes in the TN and TP removal efficiencies, depending on whether or not a settling process is applied, in a sequencing batch reactor (SBR) process with a membrane bioreactor (MBR). Nutrient removal was considered in terms of developing an advanced water treatment system for ships in accordance with water quality standards set forth by 227(64). For these purposes, the TN and TP concentrations in the inflow and outflow water were measured to calculate the TN and TP removal efficiencies, depending on whether or not a settling process was used. Water discharged from a bathroom, which was constructed for the experiment, was used as the raw water. The experiment that included a settling process was conducted twice, and the operating conditions were: aeration for 90 min, settling for 30 min, agitation for 15 min, and settling for 15 min for one experiment; and aeration for 150 min, settling for 45 min, agitation for 15 min, and settling for 15 min in the other. Operating conditions for the experiment that did not include a settling process were: aeration for 180 min and agitation for 60 min. The concentration of the mixed liquor suspended solids (MLSS) in the reactor was 3,500 mg/L, while the aeration rate was 121 L/min and the water production rate was 1.5 L/min. For the two experiments where a settling process was applied, the average TN removal efficiencies were 44.39% and 41.05%, and the average TP removal efficiencies were 47.85% and 46.04%. For the experiment in which a settling process was not applied, the average TN removal efficiency was 65.51%, and the average TP removal efficiency was 52.51%. Although the final nutrient levels did not satisfy the water quality standards of MEPC 227(64), the TN and TP removal efficiencies were higher when a settling process was not applied.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.341-352
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• 2012

In large-type goats that were fed on dry forage twice daily, dry forage intake was markedly suppressed after 40 min of feeding had elapsed. The objective of this study was to determine whether or not marked decreases in dry forage intake after 40 min of feeding are mainly caused by the two factors, that is, ruminal distension and increased plasma osmolality induced thirst produced by dry forage feeding. Six large-type male esophageal- and ruminal-fistulated goats (crossbred Japanese Saanen/Nubian, aged 2 to 6 years, weighing $85.1{\pm}4.89kg$) were used in two experiments. The animals were fed ad libitum a diet of roughly crushed alfalfa hay cubes for 2 h from 10:00 to 12:00 am during two experiments. Water was withheld during feeding in both experiments but was available for a period of 30 min after completion of the 2 h feeding period. In experiment 1, saliva lost via the esophageal fistula was replenished by an intraruminal infusion of artificial parotid saliva (RIAPS) in sham feeding conditions (SFC) control, and the treatment was maintained under normal feeding conditions (NFC). In experiment 2, a RIAPS and non-insertion of a balloon (RIAPS-NB) control was conducted in the same manner as the SFC control of experiment 1. The intraruminal infusion of hypertonic solution and insertion of a balloon (RIHS-IB) treatment was carried out simultaneously to reproduce the effects of changing salt content and ruminal distension due to feed entering the rumen. The results of experiment 1 showed that due to the effects of multiple dry forage suppressing factors when feed boluses entered the rumen, eating rates in the NFC treatment decreased (p<0.05) after 40 min of feeding and cumulative dry forage intake for the 2 h feeding period reduced to 43.8% of the SFC control (p<0.01). The results of experiment 2 indicated that due to the two suppressing factors of ruminal distension and increased plasma osmolality induced thirst, eating rates in the RIHS-IB treatment were, as observed under NFC, reduced (p<0.05) and cumulative dry forage intake for the 2 h feeding period decreased to 34.0% of the RIAPS-NB control (p<0.01). The combined effects of ruminal distension and increased plasma osmolality accounted for 77.5% of the suppression of dry forage intake 40 min after the start of dry forage feeding. The results indicate that ruminal distension and increased plasma osmolality induced thirst are the main factors in the suppression of dry forage intake in large-type goats.

• Korean Journal of Medicinal Crop Science
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• v.9 no.3
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• pp.211-219
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• 2001

The disease incidence rates of Phytophthora root rot of A. macrocephala caused by P. drechsleri were dramatically increased in two distinctive periods at experimental fields of National Crop Experiment Station(NCES), Suwon, in 1999 and in 2000 ; one was in the period of 30 to 45 days old seedling stage in spring and the other was just after heavy rainy season in late summer or in early fall. The disease was occurred at seedling stage under the conditions of ${{\geq}15^{\circ}C}$ of average temperature with ${{\geq}100mm}$ rainfalls for 20 days and the tendencies were similar in both year. By the disease, rhizome propagated field was more damaged(18.6%) than seed propagated field(56.0%). Comparing the disease incidence rates at five different fields in Suwon, Youngju and Andong, the damages at soil improving fields and non-mulching fields were less severe than those at continuous cropping fields without soil improvement and mulching fields and occurrence. Expansion of the disease were seemed to be highly related with the populations of P. drechsleri in soils depend on the cultivation method and field conditions. Although the populations of the pathogen in soils collected from Andong and Youngju, in which rhizome were continuously propagated for two and three years respectively, were comparably less than that from Suwon, in which rhizome were propagated for one year, however, the damages by the disease were more severe in Andong and Youngju. So, two or more years of cultivation at the same field may not be useful for Atractylodes plants..

• Atmosphere
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• v.25 no.4
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• pp.639-657
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• 2015

There is an increasing need to improve the air quality over South Korea to protect public health from local and remote anthropogenic pollutant emissions that are in an increasing trend. Here, we evaluate the performance of the WRF-Chem (Weather Research and Forecasting-Chemistry) model in simulating near-surface air quality of major Korean cities, and investigate the impacts of time-varying chemical initial and lateral boundary conditions (IC/BCs) on the air quality simulation using a chemical downscaling technique. The model domain was configured over the East Asian region and anthropogenic MICS-Asia 2010 emissions and biogenic MEGAN-2 emissions were applied with RACM gaseous chemistry and MADE/SORGAM aerosol mechanism. Two simulations were conducted for a 30-days period on April 2010 with chemical IC/BCs from the WRF-Chem default chemical species profiles ('WRF experiment') and the MOZART-4 (Model for OZone And Related chemical Tracers version 4) ('WRF_MOZART experiment'), respectively. The WRF_MOZART experiment has showed a better performance to predict near-surface CO, $NO_2$, $SO_2$, and $O_3$ mixing ratios at 7 major Korean cities than the WRF experiment, showing lower mean bias error (MBE) and higher index of agreement (IOA). The quantitative impacts of the chemical IC/BCs have depended on atmospheric residence time of the pollutants as well as the relative difference of chemical mixing ratios between the WRF and WRF_MOZART experiments at the lateral boundaries. Specifically, the WRF_MOZART experiment has reduced MBE in CO and O3 mixing ratios by 60~80 ppb and 5~10 ppb over South Korea than those in the WRF-Chem default simulation, while it has a marginal impact on $NO_2$ and $SO_2$ mixing ratios. Without using MOZART-4 chemical IC, the WRF simulation has required approximately 6-days chemical spin-up time for the East Asian model domain. Overall, the results indicate that realistic chemical IC/BCs are prerequisite in the WRF-Chem simulation to improve a forecast skill of local air quality over South Korea, even in case the model domain is sufficiently large to represent anthropogenic emissions from China, Japan, and South Korea.

• Korean journal of applied entomology
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• v.22 no.1 s.54
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• pp.1-6
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• 1983

The survey on the occurrence and distribution of barley stripe disease was conducted in the farmer's field of 19 gun (county) throughout Yeongmm area in May of 1982, in order to obtain a basic information on the breeding of resistant varieties, and for control of the disease. The percent of infected culms of barley stripe disease in Gyeongnam province (Southern Yeongnam) was higher $(13.7\%)$ them in Gyeong-bug $(6.9\%)$, northern Yeongnam, and especially, Ham-an, Milyang, Eui-chang and Weol-seong were severly occurred. The cultivar of Millyang 6 was slightly infected while the cultivars Olbori and Oweolbori were severely infected by the disease. Among soil conditions, the barley plant grown in the loam, clay and clay loam texture which have more availble moisture, and that of the plant cultivated in the poorly drained soils were shown to have severe infection. The barley plant grown in the soils in local valley $(18.8\%)$ where is frequently over saturated with water showed the more infection the barley plant grown in plains $(9.5\%)$. Generally, the poorer the soil drainage the more severeinfection occurred. Among cultivation conditions, the earlier the sowing dates of the barley, the less the percent of infected culms was observed. The heavier or lighter application of N fertilizer than the optimum to barley plant seemed to cause more infection.