• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.459-476
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• 2008

Taxonomic characteristics of spores of 17 species belonging to 6 genera of the Korean Thelypteridaceae are examined. Description of each species and key to the species are provided. Spores of Korean Thelypteridaceae are monad and monolete with bilateral symmetry. Perispore, the sporoderm of spore, was found in all the species examined. The perispores and exospores of Thelypteris japonica var. japonica, T. japonica var. glabrata and Pseudocyclosorus subochthodes are much similar to each other, but the rest of taxa examined shows diverse patterns of the surface ornamentation of perispores and exopores enough to distinguish genera and sometimes to distinguish species in some genera. Previous study reported that perispore is not easily distinguished from exospore in Cyclosorus and Leptogramma. However, three species of Cyclosorus examined here show different pattern having scabrate or verrucate exospores and cristate or echinate perispores. Leptogramma pozoi ssp. mollisima also have echinate perispore and fossulate exospore. Macrothelypteris oligophlebia var. elegans and M. viridifrons have scabrate exospore which is contrast to previous report of reticulate exospore. Thelypteris glanduligera, T. angustifrons and T. laxa show same patterns of perispore and exospore with reticulate perispore and fossulate exospore and hence cannot be distinguished by spores only.

• Korean Journal of Plant Taxonomy
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• v.36 no.1
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• pp.1-19
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• 2006

Description of spore morphology of Korean Polypodiaceae has on LM and SEM and key to the genera are provided. Korean Polypodiaceae monad, monolete with bilateral symmetry, bipolar and oblete spore except for Loxogramme grammitoides having trilete spore with radial symmetry. In spore wall structure, exopore showed variable patterns enough to distinguish genera. Perispore was found in all the species examined. The patternof perispore and exospore was most similar in all the species examined except Crypsinus, Pyrrosia hastata ans P. linearifolia. The perispores of Crypsinus, Pyrrosia hastata and P. linearifolia were echinate and gemmate, rugulate, and verrucate respectively. In term of exospore wall sculpturing, Polypodium and Loxogramme showed fossulate, Lepisorus, rugulate with irregular verrucate, Crypsinus ans Coloysis showed psilate or rarely verrucate in the latter, Neocheiropteris ensata showed dense small verrucate, Lemmaphyllum microphyllum showed complex reticulate and Pyrrosia showed verrucate or psilate.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.67-83
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• 1983

The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured $2.9{\pm}0.28{\mu}$ in length and $1.7{\pm}0.29{\mu}$ width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide ($H_2O_2$) at $30^{\circ}C$ was $26{\mu}$ of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of $60^{\circ}$ to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida, order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was $7.1{\times}10^7/ml$ as lower pathogenecity than that of Nosema bombycis Naegeli of $1.2{\times}10_7/ml$. While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell weight and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg batches from the infected female moth.