• 보존과학연구
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• s.28
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• pp.75-90
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• 2007

The ancient biomolecular remains are the potential source for paleobiology and paleoanthropology. Especially, ancient human specimens such as bone, teeth, and hair are powerful materials to identify historical origin and migration of ancestor population from the past. However, most excavated human specimens in archaeological sites have commonly problems as natural damage and exogenous contamination. We carried out histological and molecular analyses of excavated bone from the historic sites in South Korea from the recently discovered in tumulus of Seochun and Naju. Biological deterioration of bone was observed anatomically by optical and scanning electron microscope (SEM). We extracted degraded DNA, and amplified hyper variable region (HVR) of mitochondrial DNA (mtDNA) and amelogenin of nucleus DNA. This study applied and examined the relationships between histological preservation and DNA survival in excavated bone.

• 보존과학연구
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• s.22
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• pp.27-40
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• 2001

DNA typing is often used to determine identity from human remains. Recently, the molecular biological analysis of ancient deposits has become possible since methods for the recovery of DNA conserved in bones or teeth from archaeological remains have been developed. In the field of archaeology, one of the most promising approaches is to identify the individuals present in a mass burial site. We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from a Korea ancient human remain excavated from Sa-chon Nuk-island and civilian access controlline(CACL). A femur bone were collected and successfully subjected to DNA extraction, quantification, PCR amplification, and subsequently typed for several shot tandem repeat(STR)loci. 4 types of STR systems used in this study were CTT multiplex(CSF1PO, TPOX, TH01), FFv multiplex(F13A01, FESFPS, vWA), Silver STRⅢ multiplex(D16S539, D7S820, D13S317), and amelogenin for sex determination. This studies are primarily concerned with the extraction, amplification, and DNA typing of ancient human bone DNA samples. Also, it is suggestive of importance about closely relationship between both fields of archaeology and molecular biology.

• Korean Journal of Heritage: History & Science
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• v.41 no.1
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• pp.99-108
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• 2008

The paleogenetic analysis has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. Recently, scientific research for human skeletal remains was more activated because of increasing awareness of the valuable archaeological information by the ancient DNA analysis. State of preservation of organic remains vary in different soil and burying environmental condition. Almost all available tissue disappear to analysis ancient DNA of bone in acidic soil caused by climate and geological features in Korea. Many preserved human remains excavated in the 'Heogwakmyo'(limelayered tomb of Chosun Dynasty Period) is able to explain through the relationship between burial conditions and bone survival form the burial method and ceremony. Ancient DNA analysis of excavated human bone form ancient tomb requires to remove contaminants such as microorganism's DNA and soil components that affect authentic results. Particularly, contamination control of contemporary human DNA is major serious problem and should verified by criteria of authenticity. In order to understand migration and culture of ancient population, when possible, ancient DNA studies needs to go abreast both radiocarbon and stable isotope studies because the dietary inferences will suggest ancient subsistence and settlement patterns. Also when the paleogenetic research supported with the arts and humanities research such as physical anthropology and archaeology, more valuable ancient genetic information is providing a unique results about evolutionary and population genetics studies to reconstruct the past.

• 보존과학연구
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• s.20
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• pp.5-20
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• 1999

We have analyzed the allele and genotype frequencies from 10 fractions of ancient human skeleton in 3 pieces of Jar coffin excavated from Naju Bokamni3rd tumulus by PCR amplification, high resolution polyacrylamide gelelectorphoresis and silver staining. We could isolate human genomic DNA from 3 bone fractions but the rest of them could not be used as materials due to being decayed. We could detect sex determination as male and 3 genotypes of STR system, HUMTHO1, HUMTPOX and HUMC5F1PO from the bone fraction of left side in Jar coffin 3 and see the slightly reaction suggesting the sex as male from the bone fraction of the left side in Jar coffin 2 and female from the right side in Jar coffin 3.We have also analyzed the genotype frequencies of mitochondria from the bone fractions of the left side and the right side in jar coffin 3, respectively. From the result of indetifiying at nucletide position between 16018 and 16378of the base of hyper variable region(HV1) in the control region, We can presume that the both bones have the same maternal inheritance.

• 보존과학연구
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• s.29
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• pp.111-124
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• 2008

Gold case which is called sarari container was excavated from Wangheungsaji of Buyeo in October 2007. This case did not contain sarira, but was filled with clear liquid. This study focused on component analysis of the liquid which had filled sarira container, because we didn't know if this case had not originally contained sarira or not. It is said that original sarira can be melted by a some liquid in this case. So, many kinds of samples which are liquid, soil and ground water, was collected from a inner and outer of sarira container, and analyzed using a IC, ICP-MS, GC-MS and SEM-EDS. As a result of analysis, all of the elements composition was identified by general components of soil and ground water. Especially, phosphate which is chemical element to consist of human bone, did not found in this liquid.

• 보존과학연구
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• s.25
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• pp.47-74
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• 2004

We investigated the nucleotide substitution and insertion polymorphism of the hypervariable region Ⅰ and Ⅱ in mt DNA by sequencing ancient DNA from 51 ancient bones and teeth excavated at Nuk-do and Yimdang-dong in Korea. It revealed 35 sequence types from the ancient Korean. Of these, different sequences were 34 sequences. There were 19 and 38 base substitutions in HVI and HVⅡ, respectively. Some substitutions were characteristic of East Asian populations as compared with data reported on Caucacianpopulations,16051, 16150, 16172, 16223 in region I and 73, 263 in region II were noted as polymorphic sites, respectively. These were distributed evenly along the control region, though the frequency of each site was variable. Nucleotide substitution rather than insertion and deletion was the prevalent pattern of variation. Insertion of cytosine between312 and 315 in region HVⅡ were detected up to 98% in 51 ancient bone samples. This sequence data represents a phylogenetic tree using NTI DNA Suite computer program. The phylogenetic tree showed that mt DNA sequences of Nuk-do bones were relative to west Siberian and Indonesian. The usefulness of mt DNA sequencing in ancient Korean population excavated atarchaeological sites is based on biological and historical evidence for origin and migration of ancient Korean.

• 보존과학연구
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• s.31
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• pp.69-77
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• 2010

Preservation of artifacts that are excavated from archeological sites is closely related to soil environment. Biological remains are especially influenced by degradation activity of microorganism from soil environment. In this study a preserved human bone in archaeological tomb, Tou-kwang-myo from Joseon Period was analyzed to characterize bacteria groups by molecular genetic tools using 16S rDNA sequences. 117 clones were identified and classified 9 phylogenetic groups : ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-Proteobacteria, Sphingobacteria, Clostridia, Actinobacteridae, Nitrospiraceae, and Gemmatimonadetes according to homologous 16S rDNA sequences submitted in NCBI. ${\gamma}$-Proteobacteria group appears the highest ratio in bones (about 35%) while about 19.6% belong to the Actinobacteria group. The results may contribute to study on the effect of microorganisms on the human remains with burial method.

• Journal of Conservation Science
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• v.30 no.4
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• pp.409-416
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• 2014

Most human bones of Joseon Dynasty period are so good condition that we can do research in physical anthropology, genetics and chemistry with them. In this study, we analyzed DNA typing using 6 human bones of Joseon dynasty period excavated at Janggi-dong, Gimpo. The DNA typing was mitochondrial DNA haplotype, Y-chromosome haplotype and sex determination. Prior to DNA analysis, we distinguished histological index of 6 human bones. As the result of mitochondrial DNA analysis, most of bones were confirmed as haplogroup G, R11, M7, A5, etc. As the result of sex determination, 4 human bones were female and 2 human bones were male. The male haplogroup was confirmed as haplogroup O by the single nucleotide polymorphism analysis of Y chromosome. For extensive ancient human bone analysis, researchers need to apply a histological index to select ancient human bones and explain a relationship among ancient human bones with various analyses of mitochondrial and nuclear DNA.

• 보존과학연구
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• s.24
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• pp.153-167
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• 2003

We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from an ancient Korean remainsexcavated from Siheung in Korea. 7 bones were collected and partially STR(short tandem repeat) systems, Sex determination Amelogenin kit(Promega co, USA), were used in this study. Mitochondrial DNAs were also amplified and sequenced by ABI 310 DNA sequencer. We know that sample no. 2 and no. 3 were females and also sample no. 2 and no.7 possessed the same maternal inheritance by mitochondrial DNA sequencing results. Throughout this research, the mitochondrial DNA sequencing of human in the middle of Joseon Dynasty in Korea is obtained. In addition, this finding will be an important foundation for the future research.

• The Journal of the Korea Contents Association
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• v.7 no.9
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• pp.62-71
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• 2007

Man kind has been doing a human body ornament as a expression of desire for beauty from the earliest times. The ornament might assume expression for the human body earlier than clothes it has protected human body from the exterior such as dishonor, cold weather and hot weather etc. because ornament has been excavated compare than clothes. for example. The aborigine's clothes and tools seem to be more splendid ornament than natural function. The ornament has been development with variety such as bone from the animals, Feather from the primitive ages to high technology new materials in the modern society. In this research contribute that development of jewelry contents industries through the vernacular of tradition korean jewelry.