• Animal Systematics, Evolution and Diversity
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• v.8 no.2
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• pp.211-222
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• 1992

The systematic study of freshwater rotifers was conducted with the materials collected from 205 sites in South Korea. As a result, 4 subspecies, 3 from of Family Brachionidae (Keratella, Notholca) was identified; of which, 1 from is new to the Korean fauna: Keratella quadrata f. testudo. Total 135 species, 15 subspecies, 9 varieties and 10 forms representing 14 families 40 genera are now recorded from Korea by adding the 1 from newly described in the present paper.

• Animal Systematics, Evolution and Diversity
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• no.nspc2
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• pp.107-134
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• 1988

This study on the ampithoid gammarids in Korea was based on the materials collected in 32 localities , and deposited in the Department of Zoology, Seoul National University . Twelve species in three genera were identified and classified, of which following five species were new to science ; Ampithoe brevipalma Ampithoe Koreana, AMpithoe youngsanensis, Peramphithoe baegryeongensis, Peramphithoe namhaensis. Ampithoe shimizuensis Stephensen, 1944 was newly ranked as a subspecies - Ampithoe valida shimizuensis. Ampithoe ramondi Audouin, 1826 ,Peramphithoe tea (Barnard , 1965), Perzmphithoe orientalis (Dana, 1853) and Sunamphithoe plumosa Stephensen, 1944 are new records for Korea.

• Animal Systematics, Evolution and Diversity
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• v.33 no.2
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• pp.123-130
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• 2017

The family Geometridae, which is one of the most species-rich taxa of Lepidoptera, includes more than 23,000 species worldwide and over 800 species in South Korea. Herein, we record six species of Geometridae for the first time in Korea. Jodis urosticta and Jodis amamiensis can be characterized by their greenish wings and strongly dentate ante- and postmedial lines on the fore and hindwings. These species can also be separated by the shape of the valva of the male genitalia. Pelagodes antiguadraria can be characterized by their greenish wings, whitish medial bands on the forewing and whitish postmedial line on the hindwing. Brabira kasaii can be characterized by the thick grayish band-shaped central fascia of their fore- and hindwings. Sauris marginepunctata can be characterized by the greenish ground color of the forewing, the dark ochreous medial bands and subterminal line and the grayish hindwing. Philereme vetulata can be characterized by their dark brownish wing color and the band-shaped central fascia of the forewing, as well as the medially projected postmedial line of the hindwing. Echthrocollix minuta can be characterized by their pale grayish wing color, brownish postmedial and subterminal lines and large discal dot.

• Architectural research
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• v.21 no.1
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• pp.1-8
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• 2019

Social housing has a long history in Germany from the first still existing social housing ever, the "Fuggerei" in Augsburg (founded in 1521), over the last 100 years from the end of World War I to today's situation where the need in social housing has increased while the number of housing projects and the number of existing apartments in this program has decreased or ended. Socio-economic changes like demographic evolution, more single households, greater working abilities in bigger cities and an unforeseen highly increased number of migrants within Europe mostly but also from other countries led to the need of affordable housing for a growing number of people who are not able to care for their housing needs in their own responsibility. This is especially true for bigger cities, where the offer of affordable housing is nearly non-existing any more. The family Fugger, a trade and banking dynasty at their time, established a very modern housing concept, providing good and healthy living space for their workers. In 2018 now some trade companies, discounters (ALDI, LIDL, Norma) and IKEA announce to combine their interest in sales in the inner cities with the municipal interest of redensification of existing housing areas and conversion to ecological urban reconstructuring.

• International journal of advanced smart convergence
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• v.8 no.3
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• pp.115-122
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• 2019

Recent and ongoing discoveries of mycoviruses with new properties demand the development of an appropriate research infrastructure to analyze their evolution and classification. In particular, the discovery of negative-sense single-stranded mycoviruses is worth noting in genome types in which double-stranded RNA virus and positive-sense single-stranded RNA virus were predominant. In addition, some genomic properties of mycoviruses are more interesting because they have been reported to have similarities with the pathogenic virus family that infects humans and animals. Genetic information on mycoviruses continues to accumulate in public repositories; however, these databases have some difficulty reflecting the latest taxonomic information and obtaining specialized data for mycoviruses. Therefore, in this study, we developed a bioinformatics-based pipeline to efficiently utilize this genetic information. We also designed a schema for data processing and database construction and an algorithm to keep taxonomic information of mycoviruses up to date. The pipeline and database (termed 'mycoVDB') presented in this study are expected to serve as useful foundations for improving the accuracy and efficiency of future research on mycoviruses.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.419-428
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• 2019

Phytases are enzymes that can hydrolyze phytate and its salts into inositol and phosphoric acid, and have been utilized to increase the availability of nutrients in animal feed and mitigate environmental pollution. However, the enzymes' low thermostability has limited their application during the feed palletization process. In this study, a combination of B-value calculation and protein surface engineering was applied to rationally evolve the heat stability of Escherichia coli phytase. After systematic alignment and mining for homologs of the original phytase from the histidine acid phosphatase family, the two models 1DKL and 1DKQ were chosen and used to identify the B-values and spatial distribution of key amino acid residues. Consequently, thirteen potential amino acid mutation sites were obtained and categorized into six domains to construct mutant libraries. After five rounds of iterative mutation screening, the thermophilic phytase mutant P56214 was finally yielded. Compared with the wild-type, the residual enzyme activity of the mutant increased from 20% to 75% after incubation at $90^{\circ}C$ for 5 min. Compared with traditional methods, the rational engineering approach used in this study reduces the screening workload and provides a reference for future applications of phytases as green catalysts.

• Journal of the Korean Wood Science and Technology
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• v.47 no.3
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• pp.371-380
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• 2019

Termites are pests that cause serious economic and cultural damage by digesting wood cellulose. Termites are arthropods and have an epidermis surrounded by a chitin layer. To maintain a healthy epidermis, termites have chitinase (${\beta}$-1,4-poly-N-acetyl glucosamidinase, EC 3.2.1.14), an enzyme that hydrolyzes the ${\beta}$-1,4 bond of chitin. In this study, the amino acid sequence of the gene, which is presumed to be termite chitinolytic enzyme (NCBI accession no. KC477099), was obtained from a transcriptomic analysis of Reticulitermes speratus KMT001 in Bukhan Mountain, Korea. An NCBI protein BLAST search confirmed that the protein is a glycoside hydrolase family 18 (GH18). The highest homology value found was 47%, with a chitinase from Araneus ventricosus. Phylogenetic analysis indicated that the KC477099 protein has the same origins as those of arthropods but has a very low similarity with other arthropod chitinases, resulting in separation at an early stage of evolution. The KC477099 protein contains two conserved motifs, which encode the general enzymatic characteristics of the GH18 group. The amino acid sequences $Asp^{156}-Trp^{157}-Glu^{158}$, which play an important role in the enzymatic activity of the GH18 group, were also present. This study suggests that the termite KC477099 protein is a new type of chitinase, which is evolutionarily distant from other insect chitinases.

• Genomics & Informatics
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• v.20 no.1
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• pp.6.1-6.15
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• 2022

Litorilituus sediminis is a Gram-negative, aerobic, novel bacterium under the family of Colwelliaceae, has a stunning hypothetical protein containing domain called von Hippel-Lindau that has significant tumor suppressor activity. Therefore, this study was designed to elucidate the structure and function of the biologically important hypothetical protein EMK97_00595 (QBG34344.1) using several bioinformatics tools. The functional annotation exposed that the hypothetical protein is an extracellular secretory soluble signal peptide and contains the von Hippel-Lindau (VHL; VHL beta) domain that has a significant role in tumor suppression. This domain is conserved throughout evolution, as its homologs are available in various types of the organism like mammals, insects, and nematode. The gene product of VHL has a critical regulatory activity in the ubiquitous oxygen-sensing pathway. This domain has a significant role in inhibiting cell proliferation, angiogenesis progression, kidney cancer, breast cancer, and colon cancer. At last, the current study depicts that the annotated hypothetical protein is linked with tumor suppressor activity which might be of great interest to future research in the higher organism.

• Animal Systematics, Evolution and Diversity
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• v.40 no.2
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• pp.130-134
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• 2024

The family Velutinidae is found in various intertidal and subtidal habitats worldwide including Arctic and Antarctic seas. They are characterized by possessing a fragile shell that is partially or entirely covered by the mantle. Eight valid species of the genus Coriocella have been reported mostly in the Indo-West Pacific. Here we report Coriocella jayi Wellens, 1996 from Korean waters for the first time and describe details of their external morphology and radula characteristics using scanning electron microscopy, and provide the mtDNA cox1 sequence as a DNA barcode sequence information. This species is distinguished from other congeneric species by having six cylinder-shaped tubercular lobes of their dorsal part of mantle body and mantle color. Phylogenetic tree using the mtDNA cox1 sequence data shows that two Coriocella species (C. jayi and C. nigra) are grouped as their respective sister among Velutinidae species, and these relationships are strongly supported by 100% bootstrap value. Despite the morphological similarities, further investigation will be needed to confirm whether the African and Korean populations can be justified as the same species with a disconnected distribution range, or represent morphologically similar but two distinct species.

• Molecules and Cells
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• v.38 no.11
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• pp.950-958
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• 2015

BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in human genome. In present study, we conducted evolutionary analysis of AluYRa2-exonized transcript with various primate genomic DNAs and cDNAs from humans, rhesus monkeys, and crabeating monkeys. Remarkably, our results show that AluYRa2 element has only been integrated into genomes of Macaca species. This Macaca lineage-specific integration of AluYRa2 element led to exonization event in the first intron region of BCS1L gene by producing a conserved 3' splice site. Intriguingly, in rhesus and crabeating monkeys, more diverse transcript variants by alternative splicing (AS) events, including exon skipping and different 5' splice sites from humans, were identified. Alignment of amino acid sequences revealed that AluYRa2-exonized transcript has short N-terminal peptides. Therefore, AS events play a major role in the generation of various transcripts and proteins during primate evolution. In particular, lineage-specific integration of Alu elements and species-specific Alu-derived exonization events could be important sources of gene diversification in primates.