Search | Korea Science

Kim, Seon-Ja;Park, So-Young;Moon, Heung-Kyu;Lee, Wi-Young
- Journal of Korean Society of Forest Science
- /
- v.99 no.1
- /
- pp.125-130
- /
- 2010
The application of bioreactor culture techniques for plant micropropagation is regarded as one of the ways to reduce production cost by scaling-up and automation. In an attempt to optimize mass proliferation systems in Eucalyptus pellita, four types of bioreator systems including temporary immersion system with or without net were tested. Highest growth was achieved with 30-min flushes of medium at every 4-h intervals in TIN (temporary immersion with net) system. Results indicate over three-fold increase in shoot growth with the TIN system when compared with TIX (control: temporary immersion without net) system which is without net in bioreactor. Furthermore, plants produced from the TIN system increased total chlorophyll content, chlorophyll a/b and dry matter, giving higher yields of acclimatized plants. Our findings suggest that plantlet growth increases with appropriate exposure to media at correct intervals, as well as use of net for maintaining aerobic condition in the vessels. The TIN system thus has great potential for in vitro mass production of Eucalyptus clones commercially.
PDF KSCI