• 한국식품영양과학회지
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• 제27권4호
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• pp.687-692
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• 1998

To use onion(Allium cepa L.) residue as raw materials of food product, yields of onion-juice according to various extracting methods and optimal extracting condition of quercetin and its related glycosides were carried out. Onion juices gained by the methods of pressing, rotary crushing, freeze pressing and enzyme treatment. The yield by the method of enzyme treatment was higher than others. The yields of juice from fresh onion and heat-treated onion(8$0^{\circ}C$/10min) by pressing were 66% and 83%, respectively. Ethanol extraction of onion was efficient at 75$^{\circ}C$ and acetic acid extraction was proper at 3% concentration for 3 hours at 11$0^{\circ}C$. The onion extract was fractionated in the order of hexane, chloroform, ethylacetate and butanol to test flavonoids. The highest abundant flavonoids were found in ethylacetate and butanol fraction.

• International Journal of Oral Biology
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• 제36권3호
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• pp.149-154
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• 2011

In the present study, total methanol extracts prepared from Alpinia katsumadai showed significant protective effects against the oxidative stress induced by hydrogen peroxide, UV-C or ${\gamma}$-ray irradiation. These protective effects were substantially increased by treatment with 20~100 ${\mu}g$/ml of the extract. The A. katsumadai total methanol preparation was further fractionated into n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these five fractions, the ethylacetate and butanol fractions of A. katsumadai showed the strongest protective effects against oxidative stress induced by UV-C and ${\gamma}$-ray irradiation. These fractions also showed high DPPH radical scavenging and lipid peroxidation inhibitory activities. In addition, both fractions displayed cell proliferation activation effects, as evidenced by significant increases in colony formation. Our current data thus suggest that the mechanisms underlying the protective effects of A. katsumadai against oxidative damage may include radical scavenging, protection against cell membrane damage and stimulation of cell proliferation.

• 한국식품영양과학회지
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• 제28권2호
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• pp.458-463
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• 1999

Methanol extract and its fraction of traditional fermented soybean paste(doenjang) from Soonchang area were studied for growth suppression on the various tumor cells and suppression components, by using HPLC and GC were analysed. Hexane fraction of methanol extract was indicated 79%, 76%, 67%, 66%, 78% of growth suppression on L1210, P338D1, HepG2, WiDr and SNU 1 tumor cells, respectively. Ethylacetate fraction of methanol extract also showed 81%, 75%, 75%, 76% and 82% of growth suppression on the same tumor cells, respectively. Peak 8 obtained from HPLC of ethylacetate fraction indicated 81%, 77%, 77%, 75% and 79% of growth suppression on the same tumor cells and identified as a genistein, by comparing with standard one by HPLC analysis. Hexane fraction of methanol extract contained oleic acid, linoleic acid and palmitic acid.

• 한국식품영양과학회지
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• 제29권1호
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• pp.153-160
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• 2000

We investigated the cytotoxic effects of roots and seeds Daucus carota L. on HepG2, HeLa, MCF7, SW626, C6 and NB41A3 cell lines by the MTT assay. Among extracts, the ethylacetate partition layer (DCMEA) of root of Daucus carota L. showed the strongest cytotoxic effects on HepG2, HeLa, C6 and NB41A3 cell lines. On the other hand, methanol(DCM), n-ethylacetate(DCMEA) and n-butanol(DCMB) extract of the seeds of Daucus carota L. also showed significant cytotoxic activities for all six cell lines. $\beta$-carotene, a well-known main component of Daucus carota L. was also tested for its cytotoxic effect. However, in all six cell lines, $\beta$-carotene falied to show significant cytotoxicity. Therefore, the anticancer effect of DCMEA of root fo Daucus carota L. and DCM, DCMH, DCMEA and DCMB extracts of seeds may be caused by components other than $\beta$-carotene. Futher studies are under way to isolate the compounds responsible for the significant cytotoxic activity.

• 한국미생물·생명공학회지
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• 제24권5호
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• pp.591-596
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• 1996

In the course of screening lipid peroxidation inhibitor from basidiomycetes, a mushroom, which was collected at O-Dae mountain in Kangweon- Do, was found to have active compound. The mushroom was identified as Polyzellus multiplex, which belongs to Aphylloporalles Thelephoraceae, on the basis of macroscopic and microscopic characteristics of the fruiting body. The methanol extract of fruiting body was extracted with benzene and ethylacetate, sequentially. By using various kinds of chromatographies, PM1, and PM2 and PM3, were purified from the ethylacetate extract and the benzene extract, respectively. Color reaction and analyses of IR, UV, and NMR spectra indicated that PM1 was a derivative of thelephoric acid, and PM2 and PM3 were linoleic acid and oleic acid, respectively. IC$_{50}$ of PM1 for inhibition of lipid peroxidation was 1.96 ppm and LD$_{50}$ was 500 mg/kg.

• 식품저장과 가공산업
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• 제6권1호
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• pp.31-38
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• 2007

매실의 생리활성을 검정하여 신약개발 이나 건강기능성 식품 개발의 기초 자료 제공을 목적으로 실험한 결과는 아래와 같다. 1) methanol, water, hexane, ethylacetate 및 ether 추출물의 항균활성 검색 결과 methanol에서 가장 크게 나타났고, hexane과 ether에서는 유의적인 활성이 나타나지 않았다. 2) 품종별 차이는 적은 반면 수확시기가 늦어짐에 따라 항균활성이 증가함을 확인할 수 있었다. 3. 용매에 따른 전자공여능은 methanol > ethylacetate > water > ether > hexane 순으로 높게 나타났으며 품종간의 차이는 크지 않았다. 4) 매실 methanol 추출물을 silica gel column chromatography와 HPLC등을 이용해서 분리, 정제하였고 LC-Mass spectrum분석 결과를 LC-Mass에 내장된 Wiley Database와 비교한 결과 dl-Malic disodium salt 로 추정되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.334.2-334.2
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• 2002

As the part of our continuing study for antihyperlipidemic agents from the herbal medicinal resources. we examined the possibility of the ethylacetate fraction of Alpinia officinarum water extract in vitro and in vivo. We isolated some compounds from the ethylacetate fraction of Alpinia officinarum and measured their antihyperlipidemic activities. The active components isolated by silicagel column lmproved serum TG. HDL and LDL level in corn oil feeding and triton WR-1339 induced hyperlipidemic mice. The most active compound was 3-Methylethergalangin. Consequently. these biologically active gerbal functional foods could be used for preparing the healthy food which might diabetas. hyperlipidemia and other disease.

• 한국식품영양과학회지
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• 제16권4호
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• pp.328-332
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• 1987

한국산 표고버섯의 생것과 가열한것의 향기성분을 GC, GC-MS를 이용하여 분석한 곁과는 다음과 같다. 1) 생 표고버섯에서는 향기성분 10종을 동정하였으며 이들중 1-octen-3-ol이 71.05%, ethylacetate 1.17%, 2-octenol 1.22%, 그리고 octylalcohol 1.05%로 전체의 향기성분 중에서 74.47%를 함유하고 있었으며 2) 가열중인 것에서는 8종을 동정할 수 있었으며 1-octen-3-ol이 83.68%, ethylacetate 2.24%, 2-octenol 1.55%, octylalcohol 1.28% 및 1,2,4-trithiolane 1.91%로 전체 향기성분의 90.56%를 함유하였다. 3) 1,2,4-trithiolane과 1,2,4,5-tetrathiane은 유황화합물의 향기성분들로서 표고버섯 향기의 특징으로 인정되었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제27권4호
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• pp.281-288
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• 2001

Anticancer effect of methanol extract of Caesalpinia sappan L. on oral carcinoma (KB) and osteosarcoma (HOS) cells were investigated in this study. In order to elucidate the anticancer mechanism of Caesalpinia sappan L, we analyzed telomerase inhibitory effect of the methanol extract of Caesalpinia sappan L. In addition we prepared 5 fraction samples according to its polarity differences and analyzed anticancer effects on oral carcinoma and osteosarcoma cells. Following results are obtained in this study. 1. 50% cell proliferation inhibitory value ($IC_{50}$) of the methanol extract of Caesalpinia sappan L. against oral carcinoma (KB) cells and osteosarcoma (HOS) cells were $9.0{\mu}g/ml$ and $10.9{\mu}g/ml$, respectively. 2. The methanol extract of Caesalpinia sappan L. showed inhibitory effect of telomerase which is required for cancer cell immortality. Therefore, it seems that the anticancer effect of methanol extract of Caesalpinia sappan is at least partially due to telomerase inhibitory effect. 3. Five fraction samples were prepared according to its polarity and 88.7% of ingredient of total methanol extract was transferred to ethylacetate fraction. Thin layer chromatography analysis showed that dichloromethane fraction contained ingredient with relatively high polarity and ethylacetate fraction contained similar ingredient found in total methanol extract. 4. Anticancer effect was observed in n-hexane, dichloromethane, and ethylacetate fractions. The highest anticancer effect was found in dichloromethane fraction which had $IC_{50}$ value of 4.4 and $>4.0{\mu}g/ml$ against oral carcinoma (KB) cells and osteosarcoma (HOS) cells, respectively.

• Applied Biological Chemistry
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• 제30권3호
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• pp.264-271
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• 1987

전통약주(傳統藥酒)인 백하주(白霞酒), 녹파주(綠波酒), 동동주(酒), 청명주(淸明酒)를 양조(讓造)하여 휘발성(揮發性) 향기성분(香氣成分)을 head space gas로 포집(捕集)하여 분석(分析)하였다. 향기성분(香氣成分)으로 formaldehyde, acetone, diacetyl, acetaldehyde, ethyl acetate, ethanol, propanol, iso-butanol, iso-amyl alcohol, dimethylsulfide가 분리(分離) 정량(定量)되었으며 acetaldehyde, ethyl acetate, n-propanol은 각주품(各酒品)에 따라 $17.84{\sim}73.74ppm$으로 acetaldehyde는 동동주(酒)가 62.04ppm, ethylacetate는 녹파주(綠波酒)가 41.26ppm, n-propanol은 녹파주(綠波酒)가 73.74ppm으로 가장 많았으며 iso-butanol과 iso-amyl alcohol은 청명주(淸明酒)가 각각(各各) 194.21ppm, 925.04ppm으로 가장 높았으며 백하주(白霞酒)는 72.12ppm 및 418.14ppm으로 가장 낮았다. Formaldehyde와 acetone은 $0{\sim}2.15ppm$으로 백하주(白霞酒)에서는 acetone이 정량(定量)되지 않았다. Dimethyl sulfide의 함량(含量)은 주품(酒品)에 따라 $5{\sim}65ppb$로써 동동주가 65ppb로 가장 많았으며 diacetyl은 $0.032{\sim}1.012ppm$으로 주품(酒品)에 따라 차(差)가 컸다.