• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.504-508
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• 1991

$1.85\times 10^{-10}$ (mmole/cell/h) of specific glucose consumption rate was obtained under fed-batch cultivation of recombinant mamalian ce11s with maintaining $4.7\times 10^{-7}(\mu g/ceil/h)$ of average specific erythropoeitin production rate. Higher maximum cell density was also achieved than for both cases of batch and perfusion cultivations. It proves that glutamolysis dominates metaboiic pathways at latter period of cultivation where quasi steady state was maintained. Substrate limitation of glucose concentration was estimated as 13 (mmole/l) under fed-batch conditions. while specific product production rate was decreased according to cultivation time, erythropoeitin production was increased as glucose concentration in the media increased up to 13.2 (mole/l).

• KSBB Journal
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• v.4 no.1
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• pp.34-39
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• 1989

Growth kinetics of mammalian cell, Chinese Hamster Ovalry(CHO) was investigated to effectively produce pharmaceutically important Erythropoeitin under perfusion chemostat conditions. Perfusion rate, D is correlated with total viable is to be an essential factor in controlling growth kinetic parameters under this kind of operations. It is also found that the measurement of oxygen uptake rates is a relatively accurate method to understand cell growth, in case that the traditional cell count method is no longer useful due to heavy cell clumpings. True growth yield, Ymax and maintenance coefficient, me associated with mammalian cell growth were estimated as $2.86{\times}10^8$ cells/ g of glucose and 0.0063 g of glucose/ cells/ hr, respectively.

• Journal of Yeungnam Medical Science
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• v.11 no.1
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• pp.82-93
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• 1994

Clinical study was carried out on the 64 hemodialysis patients(HD) with chronic renal failure who had been treated from December 1992 to July 1993 in Yeungnam University Hospital. The following results were obitained. In hematologic parameters, MCH was $28.8{\pm}2.0pg$, and MCV was $92.4{\pm}4.7fl$. Result revealed normochromic and normocytic anemia. Mean values of serum ferritin were $657.4{\pm}292.0ng/ml$ in men and $511.5{\pm}370g$ in women. Mean valuses of serum iron were $145.5{\pm}63.7{\mu}g/dl$. Mean values of transferrin saturation was $61.6{\pm}28.4%$. Serum frerritin, serum iron and transferrin saturation were higher in HD group than normal reference. In erythropoeitin treatment group, Hb and Hct were significantly higher than non-erythropoietin treatment group. Amount of transfusion was significantly higher in non-erythropoietin treatment group than erythropoeitin treatment group(p<0.05). Values of iron, transferrin saturation were significantly higher in abnormal liver function test(LFT) hemodialysis group than normal LFT group(p<0.05). Transfusion amounts revealed positive correlation with ferritin(r=0.4675), transferrin satruation (r=0.3823) and iron(r=0.3386)(p<0.05). In conclusion, erythropoietin treatment cna reduce requirement of blood transfusion and transfusion related side effects such as iron overload, hemosiderosis and hemochromatosis.