• Title/Summary/Keyword: erythrocyte deformability

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The Relationship between Metabolic Syndrome and Erythrocyte Deformability in Small Vessel Disease Stroke Patients (중풍환자에서 대사증후군과 적혈구변형능의 관련성)

  • Leem, Jung-Tae;Park, Su-Jyung;Kim, Mi-Young;Choi, Won-Woo;Jung, Woo-Sang;Cho, Ki-Ho;Park, Sung-Wook;Ko, Chang-Nam;Lee, Jung-Sup
    • The Journal of Internal Korean Medicine
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    • v.30 no.4
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    • pp.761-771
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    • 2009
  • Objectives : The aim of this study was to assess the relationship between metabolic syndrome and erythrocyte deform ability in acute stroke patients. Methods : Among 88 of the recruited patients, 52 were diagnosed as metabolic syndrome. We assessed their general characteristics, risk factors. We compared the assessed variables between metabolic syndrome and control group. We analyzed the relationship between metabolic syndrome and erythrocyte deform ability. We analyzed relationship between cardiovascular risk factors and erythrocyte deformability. Results : The general characteristics waist and hip circumference, waist/hip ratio were higher in metabolic syndrome group. The metabolic syndrome group was also diagnosed with hypertension, DM, and hyperlipidemia more often than the control group. The blood test metabolic syndrome group showed higher triglycerides, total lipids, fasting blood sugar, and 2 hours postprandial plasma glucose level and lower HDL-cholesterol than the control group. There were more patients diagnosed with Dampness-Phlegm in the metabolic syndrome group. There were more patients showing lower erythrocyte deform ability in the metabolic syndrome group. The plasma homocysteine level was negatively correlated with erythrocyte deform ability. Conclusion : The results reconfirmed that the risk factors are more in metabolic syndrome group. The results indicated that metabolic syndrome lead to a lower erythrocyte deform ability in small vessel disease stroke patients. The Plasma homocysteine level was negatively correlated with erythrocyte deform ability.

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The Effects of Boyanghwanotang on the Thrombosis Related Factors (보양환오탕(補陽還五湯)이 혈전생성관여인자(血栓生成關與因子)에 미치는 영향(影響))

  • Park, Jong-Hyung;Han, Yang-Hi;Jun, Chan-Yong;Kim, Dong-U;Park, Se-Gi;Lee, Chung-Jung-Hye;Go, Seung-Hi;Go, Jae-Chul;Choi, You-Kyung;Park, Sun-Young
    • The Journal of Internal Korean Medicine
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    • v.21 no.5
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    • pp.829-837
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    • 2000
  • Purpose : To investigate the inhibitory effect of BHE on platelet aggregation and erythrocyte deformability, we performed following experiments. Methods : Concentrated Boyanghwanotang (BHE) was fed to rats for 10days. High dose group was treated with $500{\beta}{\partial}/kg$ and low dose group with $500{\beta}{\partial}/kg$. Results : BHE enhanced the blood filtration rate, reduced the platelet aggregability, inhibited granule release from collagen-stimulated platelet, the prostaglandin syn-thesis in platelet and enhanced erythrocyte deformability. Conclusion : BHE should inhibit thrombos formation due to platlete hyperaggregabili-ty and reduction of erythrocyte deformabi-lity.

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Bio-inspired Cell Deformability Monitoring Chips Based on Strain Dependent Digital Lysis Rates (미소유로의 길이에 따른 통과세포의 파괴율을 바탕으로 한 생체모사 세포 변형성 검사칩에 관한 연구)

  • Youn, Se-Chan;Lee, Dong-Woo;Cho, Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.32 no.10
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    • pp.844-849
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    • 2008
  • We present a novel cell deformability monitoring chip based on the digitally measured cell lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple cell deformability monitoring by automated high-throughput testing system. We suggest the filter design considering the areal strain imposed on the cell membrane passing through the filter array having gradually increased orifice length. In the experiment using erythrocytes, we characterized the cell deformability in terms of average fracture areal strain which was $0.24{\pm}0.014\;and\;0.21{\pm}0.002$ for normal and chemically treated erythrocytes, respectively. We also verified that the areal strain of 0.15 effectively discriminates the deformability difference of normal and chemically treated erythrocytes, which can be applied to the clinical situation. We compared the lysis rates and their difference for the samples from different donors and found that the present chips can be commonly used without any calibration process. The experimental results demonstrate the simple structure and high performance of the present cell deformability monitoring chips, applicable to simple and cost-effective cell aging process monitoring.

The Effect of Woohwangcheongsim-won on Circulatory Disturbance in Diabetes (우황청심원이 당뇨병 Rat의 혈액순환장애에 미치는 영향)

  • 황성록;정승현;신길조;이원철
    • The Journal of Korean Medicine
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    • v.23 no.2
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    • pp.164-179
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    • 2002
  • Object: Death rate due to hypertension, atherosclerosis, ischemic heart disease and cerebral infarction induced by Westernized diet and increased average life span is on the rise. Decrease in blood circulation, activation of thrombus generation and intravascular lipid accumulation, cited as the principal causes of the above mentioned diseases in recent studies, result in circulatory disturbance and blood vessel obstruction leading to ischemic cell death of heart, brain and peripheral vessels. Method: We investigated the biochemical changes in microvascular permeability, aggregation of platelet and the intravascular lipid accumulation in induced-diabetic rat using Streptozotocin. We also studied the effects of Woohwangcheongsirn-won after oral administration on blood circulation, platelet function and lipid metabolism. The results are as follows: I. Woohwangcheongsim-won increased blood circulation in microvessels. 2. Woohwangcheongsim-won increased the reduced erythrocyte deformability in diabetes. 3. Woohwangcheongsim-won induced the reduction of contents of 2, 3-DPG, but failed to affect the reduced contents of ATP in erythrocyte in diabetes. 4. Woohwangcheongsim-won reduced the activity of Ca/sup 2+/-ATPase in the membrane of erythrocyte. 5. Woohwangcheongsim-won reduced the platelet aggregation evoked by platelet agglutinin factor. 6. Woohwangcheongsim-won reduced the production of platelet-derived granules. 7. Woohwangcheongsim-won reduced the production of metabolites of arachidonic acid in diabetes, and also reduced the production of increased thromboxane B2. 8. Woohwangcheongsim-won reduced the synthesis of oxidized LDL-cholesterol. In conclusion, Woohwangcheongsim-won enhanced blood circulation in microvesseles, erythrocyte deformability and inhibited the increased platelet aggregation and the synthesis of oxidized LDL-cholesterol in diabetes. Therefore Woohwangcheongsim-won is believed to positively affect blood circulation (J Korean Oriental Med 2002;23(2):164-179)

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The Stabilizing Effects of Phospholipids on the Human Erythrocyte Membranes (인체적혈구막(人體赤血球膜) 안정화(安定化)에 미치는 인지질(燐脂質)의 영향(影響))

  • Kim, Yong-Ki;Kim, Jae-Back
    • Journal of Pharmaceutical Investigation
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    • v.11 no.2
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    • pp.1-10
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    • 1981
  • Phospholipids were examined for their capacity to protect human erythrocytes against hemolysis induced by hypotonic solution, p-hydroxymercuribenzoate or hematin. The following results were obtained. 1. Phosphatidyl choline, lysophosphatidyl choline and phophatidyl ethanoleamine as well as chlorpromazine prevented the osmotic hemolysis of human erythrocytes which occurred due to water influx into erythrocytes from medium, but showed no effect on hematin-induced hemolysis which occurred without the volume change of erythrocytes. 2. Human erythrocytes were found to be most sensitive to the antihemolytic action of phospholipids among mammalian erythrocytes from sheep, rabbit, rat and mouse. 3. Phospholipids at the concentrations showing their strong antihemolytic effect on human erythrocytes against osmotic hemolysis had no influence on methylene blue uptake and volume change of erythrocytes in hypotonic solution. 4. Phospholipids increased erythrocyte deformability 2 to 3 times over control group and there was aclose relationship between their antihemolytic action and increase of deformability as a function of their concentrations. 5. The phospholipids increased the resistance to osmotic hemolysis of human erythrocytes by increasing membrane elasticity through their incorporation into lipid bilayer without altering glucose metabolism and water influx to erythrocytes.

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The effects of Sa-Mul-Tang (Si-Wu-Tang), a Traditional Chinese Medicine, on Phenylhydrazine-induced Anemic Rats

  • Ryu, Jong-Hoon;Yook, Chang-Soo
    • Biomolecules & Therapeutics
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    • v.9 no.1
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    • pp.1-6
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    • 2001
  • Sa-Mul-Tang (Si-Wu-Tang, SMT), a kind of Chinese medicine, has been used for the hemato-deficient disease for hundreds of years. In this work, investigations on the anti-anemic activity of an aqueous extract of SMT were undertaken in order to find the pharmacological basis for the ethnomedical use of the formulation. Three kinds of Angelicase species, such as Angelica sinensis, Angelica acutiloba, and Angelica gigas, were used for preparing the water extracts of SMT. Anemic model rats were induced by the treatment of phenylhydrazine (40 mg/kg/day, i.p.) for 4 days. After the treatment of phenylhydrazine, rats were divided into several groups for their different treatment of three kinds of SMT. Red blood cell (RBC), hemoglobin (Hg), and hematocrit (Hct) were determined on the day 0, 3, 6, 10, 14 after the treatment of SMTs and erythrocytes deformabilities were also determined at the end of experiments. Oral administration of SMT (1g/kg/day) for 14 days did not ameliorate drug-induced anemic states evaluated by RBC counts, Hg contents, and Hct values. However, the erythrocyte deformabilities were improved in phenylhydrazine-treated group by the administragest that SMTs (p<0.05). Especially, these effects were high in the Angelica acutiloba group. These results suggest that SMTs have an ameliorative effect on blood rheology related to the blood stasis syndrome in oriental diagnostics not on the blood deficient states related to the anemic syndrome.

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