• Microbiology and Biotechnology Letters
• /
• v.20 no.4
• /
• pp.422-429
• /
• 1992

The effect of various water-activity depressors, such as pol yo Is, sugars, and polymers, on the conversion yields of the enzymatic synthesis of maltosyl-$\beta$-cyclodextrin from $\beta$-cyc1odextrin and maltose through reverse reaction of pullulanase was investigated. PEG 6000 of concentration of 10% (w/w) was found to be the most acceptable water-activity depressor resulting for increment of conversion yield from 43.0% to 55.9%, corresponding maltosyl-$\beta$-cyc1odextrin concentration of 3.02 g/100 ml H20. Water activity was changed from initial 0.966 to 0.914 upon addition of 20% (w/w) of PEG 6000. The conversion yields were inversely proportional to the water activities, and the increased conversion yield was caused by water activity depression which inhibited the hydrolysis reaction of maltosyl-$\beta$-CD to maltose and $\beta$-cyc1odextrin. The changes of enthalpy ($\Delta$H), entropy ($\Delta$S), and Gibbs free energy ($\Delta$G) were calculated to be 36.788 kJ/mole, 0.067 kJ/mole K. and 14.433 kJ/mole, respectively. The synthesis of maltosyl-$\beta$-CD could be increased substantially by the intermittent feeding of $\beta$-cyclodextrin. PEG 6000 could be separated effectively from reaction mixture using ultrafiltration membrane for reutilization.

• Journal of the Korean GEO-environmental Society
• /
• v.20 no.11
• /
• pp.11-22
• /
• 2019

Limit state design (LSD) and allowable stress design (ASD) are two main types of soil nailing design methodologies. In the LSD method, stability is determined by applying individual coefficients to ground strength, working load and etc. The ASD method calculates the safety factor and compares it with the minimum safety factor to determine the stability. The global design trend of soil nailing system is changing from the ASD method to the LSD method. The design method in Korea still adopts the ASD philosophy while others mostly do the limit state design. In this study, four soil nail design methods, 'FHWA GEC 7' in U.S. (2015), 'Clouterre' in France (1991), 'Soil nailing - best practice guidance' in U.K. (2005), 'Geoguide 7' in Hongkong (2008), and 'Design guide for slope in construction work' in Korea (2016) were applied to the evaluation of the stability and the results were analyzed comparatively in brief. It is revealed that the design method of 'the overall stability of soil nail walls' in Korea is the most conservative and next those by FHWA, Clouterre and CIRIA become more conservative in order. However, the difference of results obtained from FHWA and Clouterre is negligible. Also, this study found out that efforts to improve domestic design criterion are needed.