• 대한약리학회지
• /
• 제11권2호
• /
• pp.9-17
• /
• 1975

The adrenergic blocking activity and refractory period of cardiac muscle on isolated rabbit atria were measured after administration of Scutellaria. In rabbits and cats the antiarrhythmic action of Scutellaria on atrial and ventricular arrhythmias produced by epinephrine or ouabain was examined and also compared with that of propranolol and quinidine. The alcoholic extract of Scutellaria produced a marked decrease in heart rate and contractile amplitude of the isolated rabbit atria. Pretreatment with Scutellaria rendered the atria to fail to respond to epinephrine, indicating that this crude drug possesses an adrenergic blocking activity. The extract produced a marked prolongation of the refractory period of atrial muscle. The extract effectively abolished the spontaneous arrhythmia occurring in the isolated rabbit atria. As propranolol and quinidine it also suppressed the atrial arrhythmia induced by ouabain. The extract prevented, as propranolol and quinidine, the induction of ventricular arrhythmia arising from excessive dose of epinephrine in anesthetized rabbits and cats. With regard to the ventricular arrhythmia induced by a continuous infusion of ouabain, the alcoholic extract of Scutellaria exerted some suppressive effect in anesthetized rabbits but no effect on cats. From the above results, it may be concluded that Scutellaria is effective against atrial and ventricular arrhythmias. The antiarrhythmic effects of this drug may be the result of adrenergic beta receptor blocking and cardiac depressive activities including prolongation of the refractory period of cardiac muscle.

• The Korean Journal of Physiology and Pharmacology
• /
• 제5권6호
• /
• pp.503-510
• /
• 2001

Long-term treatment of cultured bovine adrenal medullary chromaffin (BAMC) cells with arachidonic acid $(100\;{\mu}M),$ angiotesnin II (100 nM), prostaglandin $E_2\;(PGE_2;\;10\;{\mu}M),$ veratridine $(2\;{\mu}M)$ or KCl (55 mM) for 24 hrs increased both norepinephrine and epinephrine levels in the supernatant. Pretreatment with staurosporine (10 nM), a protein kinase C (PKC) inhibitor, completely blocked increases of norepinephrine and epinephrine secretion induced by arachidonic acid, angiotensin II, $PGE_2,$ veratridine or KCl. In addition, K252a, another PKC inhibitor whose structure is similar to that of staurosporine, effectively attenuated both norepinephrine and epinephrine secretion induced by arachidonic acid. However, K252a did not affect the catecholamine secretion induced by angiotensin II, $PGE_2,$ veratridine or KCl. Our results suggest that staurosporine may inhibit long-term catecholamine secretion induced by various secretagogues in a mechanism other than inhibiting PKC signaling. Furthermore, long-term secretion of catecholamines induced by arachidonic acid may be dependent on PKC pathway.

• 동의생리병리학회지
• /
• 제17권4호
• /
• pp.930-938
• /
• 2003

The purpose of the present study was to investigate the effects of Hyunhosaiksan (HHS) on antithrombotic actions which include blood activation, thrombus removal, warming of circulating blood, and the control of pain on abdomen and lower and upper burning spaces. HHS significantly inhibited platelet aggregation induced by ADP and epinephrine in a HHS dose-dependent manner when analyzed by the Sigmoid Emax model in WinNonlin. EC50 values of HHS were 1.71 ㎍/ml and 0.004 ㎍/ml for ADP and epinephrine respectively. In the vivo study, HHS inhibited pulmonary embolism induced by collagen and epinephrine, which was however statistically insignificant. HHS increased number of platelets, APTT and volume of fibrinogen significantly as compared with the control group in dextran-induced thrombus model. Furthermore, HHS stimulated levels of blood flow in vivo though its effect was not observed in vitro. These results suggest that Hyunhosaiksan (HHS) can be used for treating numerous diseases related with blood aggregation and circulation problems. Further systematic investigations on the synergic effects among drugs used in the oriental medicine as well as in the western medicine in relation to thrombosis therapy would provide an important insight into the potential therapeutic applications.

• 대한정형도수물리치료학회지
• /
• 제27권2호
• /
• pp.87-92
• /
• 2021

Purpose: This study aimed to investigate the effects of myofascial release technique on pain threshold and hormonal changes in patients with adhesive capsulitis of the shoulder. Methods: Eight patients with adhesive capsulitis were treated with the myofascial release technique. Myofascial release is a form of manual therapy that involves the application of a low load, long duration stretch to the myofascial complex, intended to restore optimal length, decrease pain, and improve function. Blood tests and pressure pain threshold (PPT) examinations were performed on their first visit. On their second visit, the myofascial release technique was applied to the shoulder for 20 min. Then, blood tests and PPT were re-evaluated to determine the effects of the myofascial release technique on pain threshold and hormonal changes. Results: Pain threshold increased from 2.92 to 24.13 lb after treatment. Epinephrine decreased from .13 to .08 ng/mL whereas norepinephrine increased from .25 to .41ng/㎖ after treatment. Conclusion: Myofascial release technique in patients with adhesive capsulitis increased pain thresholds, norepinephrine and decreased epinephrine levels.

• 대한약리학회지
• /
• 제24권1호
• /
• pp.1-10
• /
• 1988

카테콜아민 생합성에 관여하는 마지막 효소인 phenylethanolamine N-methyltransferase는 Norepinephrine을 epinephrine으로 전환시키는 중요한 효소이다. PNMT효소의 발현은 epinephrine 신경세포의 발현에 필수적이다. 따라서 PNMT유전자를 크로닝하여 그 구조를 결정하고, 유전자 발현연구를 하는 것은 상당히 중요한 일이다. 그러나 최근에 저자가 bovine cDNA를 처음으로 분리하여 그 구조를 보고한 것 외에는 아직까지 인간 PNMT cDNA나, 전체 genomic DNA의 분리 보고는 없다. 이에 저자들은 인간 PNMT유전자의 전체구조와 여러 종(species) 사이의 진화적인 관계를 규명하기 위해서 human genomic library(Charon 4A)를 만들고, 이 library 이용하여 bovine cDNA를 probe로 13.1 Kb길이의 genomic clone을 분리 크로닝하는데 성공하였다. 이 유전자는 두개의 EcoRI site가 포함되어 있어서, EcoRI제한효소에 의해서 7.5 Kb, 5.0 Kb,0.6 Kb로 분리되었으며, Southern과 dot blot 실험 에서 보면 5.0 Kb와 0.6 Kb에 exon이 흩어져 존재하고 있으며, 7.5 Kb는 flanking sequence로 판명되었다.

• 대한약리학회지
• /
• 제23권2호
• /
• pp.87-94
• /
• 1987

스트레스로 인한 위궤양형성에 중추성 교감신경의 영향여부를 추구하기 위하여 norepinephrine, epinephrine, dopamine, isoproterenol 및 clonidine을 흰쥐의 뇌실내로 투여 하고 한냉 환경 $(4^{\circ}C)$에서 4시간 구속방치하여 위 분비기능의 변동과 궤양 발생 정도를 검색하여 다음과 같은 결과를 얻었다. 1. Norepinephrine, epinephrine, dopamine및 소량의 clonidine 처치로 궤양 발생이 현저하게 감소하였다. 2. Norepinephrine또는 epinephrine 처치군에서는 위액분비, 산분비 및 펩신 분비의 감소와 궤양 발생 감소가 초래되었다. 3. Dopamine혹은 소량의 clonidine 처치군에서는 궤양 발생의 감소와 위액분비 및 산분비 감소가 초래되었으나 펩신 분비는 변동 없었다. 4. Isoproterenol처치군에서는 궤양 발생과 펩신 분비는 대조군과 차이 없고, 위액분비 및 산분비의 감소만 나타났다. 5. 대량의 clonidine 투여군에서는 궤양발생, 산분비 및 펩신분비 모두 변동없이 약간의 위액분비 감소가 나타났다 이상의 결과로 보아 중추성 교감신경자극은 궤양 형성을 억압하는 작용이 있고, 이에는 교감신경성 ${\alpha}$-수용체 및 도파민성 수용체가 관여된다고 믿어지며, 이 효과는 위액분비 감소 및 산 분비 감소작용과 아울러 또 다른 요인이 관여한다고 추측된다.

• 대한약리학회지
• /
• 제16권1호
• /
• pp.41-50
• /
• 1980

Chenodeoxycholic acid(CDCA) has been used as a gallstone dissolving agent since 1972. Recently, ursodeoxycholic acid(UDCA) has been reported to be effective in dissolving gallstones. Both bile acids increased bile flow. The increase in bile flow associated with an increase in cholesterol level in bile after CDCA or UDCA infusion was reported. In this study, using the smooth muscle strips of guinea pig and fowl, responses of the cholates were observed. In addition, the influence of adrenergic blocking agents on the response of the strips to cholates was investigated. Also the effects of cholates on cardiac function were examined by using isolated atria of rabbit and heart of anesthetized frog. The results are as follows: 1) All cholates, such as UDCA, CDCA, and CA produced a marked inhibitory effect on the motility in isolated duodenal strip of guinea pig and fowl, however, only UDCA showed the contraction in the isolated esophagus of fowl. These effects of cholates were blocked by propranolol. 2) In isolated guinea pig stomach strip and gall bladder, cholates exhibited a marked inhibitory effect on the motility and the effects due to UDCA and CA were blocked by phenoxybenzamine while CDCA was not affected. 3) The spontaneous and ouabain induced arrhythmia was partially abolished by cholates. However, concomitant administration of cholates with ouabain or epinephrine caused a marked prolongation in occurrence of atrial arrhythmia in comparison with ouabain or epinephrine alone in isolated rabbit atria. 4) In the heart of anesthetized frog, the epinephrine-induced arrhythmia was partially abolished by cholates. The combined treatment with cholates and ouabain or epinephrine produced a marked prolongation in occurrence of the arrhythmia in comparison with, ouabain or epinephrine alone. From the above results, it can be suggested that the effects of cholates on the smooth muscle of duodenum and esophagus are produced in response to adrenergic ${\beta}$-receptor and the effect or gall bladder and stomach is more likely due to the direct effect on the muscle. In addition, cholates exhibit a slight antiarrhythmic effect on heart, therefore, cholates can be classified as a nonselective antiarrhythmic drug, such as propranolol.

• Journal of Ginseng Research
• /
• 제11권2호
• /
• pp.145-252
• /
• 1987

Isolated rat adipocytes are well known to possess opposite pathways of lipid metabolism: lipolysis and ipogenesis. Both of the metabolism respond to various biologically active substances such as epinephrine, ACTH and insulin. Epinephrine and ACTH stimulate lipolysis and insulin accelerates lipogenesis. Recently, Korean red ginseng powder was found to contain adenosine and an acidic poptide which inhibited epinephrine-induced lipolysis and sl imulated insulin-mediated lipogenesis from added glucose. The acidic peptide is consisted mainly of glutamic acid and glucose. Ginsenosides Rb1 and Re inhibited ACTH-induced lipolysis in isolated rat adipocytes, while they did not affect insulinstimulated lipogenesis, Thus, all these substances extracted from Korean red ginseng exhibited selective modulations toward the opposite metabolic pathways in rat adipocyte; They inhibited the lipolysis but not the lipogenesis. We call these substances"selective modulators". Recently, we isolated a toxic substance named "toxohormone-L " from ascites fluid of patients with various malignant tumors. The toxohormone-L stimulated lipolysis in rat adipocytes and induced anorexia in rats. Both the lipolytic and the anorexigenic actions of toxohormone-L were found to be inhibited by ginsenoside Rb2 in Korean red ginseng. Based on these results, physiological signifi¬cances of these substances in Korean red ginseng were discussed. Pan ax ginseng is a medicinal plant long used in treatment of various pathological states including general complaints such as head ache, shoulder ache, chilly constitution and anorexia in cancer patients, There have been many pharmacological studies on Panax ginseng roots. Petkovllreported that oral administration of an aqueous alcoholic extract of ginseng roots decreased the blood sugar levtl of rabbits. Saito2lreported that Panax ginseng suppressed hyperglycemia induced by epinephrine and high carbohydrate diets. These findings suggest that Panax ginseng roots contain insulin-like substances. Previously, we demonstrated that gin¬seng roots contain an insulin-like peptide which inhibits epinephrine-induced lipolysis and stimulated insulin-mediated lipogenesis. In 1984, we suggested that such an insulin-like substance should be called a selective modulator4). Present investigation describes the details of the selective modulators in ginseng roots. During progressive weight loss in patients with various neoplastic disease, depletion of fat stores have been observed. The depletion of body fat during growth of neoplasms is associated with increase in plasma free fatty acids. Recently, we found that the ascites fluid from patients with hepatoma or ovarian tumor and the pleural fluid from patients with malignant lymphoma elicited fatty acid release in slices of rat adipose tissue in vitro. The lipolytic factor, named"toxohormone-L". was purifed from the ascites fluid of patients with hepatoma. The isolated preparation gave a single band on both disc gel electrophoresis and sodium dodecyl sulfate(SDS)-acrylamide gel electrophoresis in the presence of ${\beta}$-mercaptoethanol. Its molecular weight was determined to be 70,000-75,000 and 65,000 by SDS-acrylamide gel electrophoresis and analytical ultracentrifugation, respectively. Injection of toxohormone-L into the lateral ventricle of rats significantly suppressed food and water intakes. There was at least 5 hr delay between its injection and appearance of its suppressive effect. In the present study, we also tried to find a inhibitory substance toward toxohormone-L from root powder of ginseng.

• 대한안과학회지
• /
• 제59권12호
• /
• pp.1137-1141
• /
• 2018

목적: 증식당뇨망막병증에서 유리체절제술과 수정체유화술을 동시에 시행한 경우 백내장수술 후 에피네프린 앞방내 주사가 동공 확대에 미치는 영향에 대해 알아보고자 하였다. 대상과 방법: 증식당뇨망막병증에서 유리체절제술과 수정체유화술을 동시에 시행한 96명(96안)에서 백내장수술 후 0.001% 에프네프린을 앞방내 주사를 한 군(주사군, 46안)과 하지 않은 군(대조군, 50안)에서 수술 전, 백내장수술 후, 유리체절제술이 끝난 후 동공 크기를 비교하였다. 동공 크기는, 저장된 수술 동영상에서 Image J 프로그램(National Institute of Health, Bethesda, MD, USA)을 이용하여 동공면적과 각막면적의 비를 구하여 수술 전-백내장수술 후의 동공 크기의 변화와 백내장수술 후-유리체절제술 후의 동공의 크기 변화를 비교하였다. 결과: 주사 군에서 수술 전 동공/각막면적의 비는 $0.52{\pm}0.11$, 백내장수술 후에는 $0.43{\pm}0.12$, 유리체절제술 후에는 $0.51{\pm}0.11$로 통계학적으로 유의한 동공 크기 변화를 관찰할 수 있었다(p<0.001, p<0.001). 대조군에서 수술 전 동공/각막면적의 비는 $0.52{\pm}0.10$, 백내장수술 후에는 $0.39{\pm}0.15$, 유리체절제술 후에는 $0.43{\pm}0.15$였다. 대조군에서는 백내장수술 후에는 동공 크기가 유의하게 차이가 났으나(p=0.011), 유리체절제술 후에는 유의한 동공 크기의 차이를 관찰할 수가 없었다(p=0.056). 결론: 에피네프린 앞방내 주사는 증식당뇨망막병증 환자에서 유리체절제술과 초음파유화술 병합 수술 시 백내장수술 후 동공을 다시 빠르게 산동시킬 수 있는 간단한 처치 방법으로 생각된다.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• 제19권4호
• /
• pp.276-280
• /
• 2016

Dieulafoy lesions, vascular anomalies typically found along the gastrointestinal tract, have been viewed as rare and obscure causes of sudden intestinal bleeding, especially in pediatric patients. Since their discovery in the late 19th century, the reported incidence has increased. This is due to an increased awareness of, and knowledge about, their presentation and to advanced endoscopic diagnosis and therapy. Our patient was a three-year-old male, without a complex medical history. He presented to the emergency department with acute hematemesis with blood clots and acute anemia requiring blood transfusion. Endoscopy revealed four isolated Dieulafoy lesions along the lesser curvature of the stomach, which were treated with an epinephrine injection. The Dieulafoy lesion, although thought to be rare, should be considered when investigating an acute gastrointestinal bleed. These lesions have been successfully treated endoscopically. Appropriate anticipation and preparation for diagnosis and therapy can lead to optimal outcomes for the pediatric patient.