• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.6
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• pp.57-65
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• 2015

Microfibrillated cellulose (MFC) or Nanofibrillated cellulose (NFC) has been used to reduce the use of raw pulp and to improve paper strength. The problem of MFC preparation is high manufacturing cost. In this study, it was carried out to prepare MFC after enzyme beating and estimated properties of MFC. Endo-D was the best beating efficiency among three type of endo-glucanase. As the grinder pass number increased, the viscosity and the fines of MFC suspension increased while the crystallinity and the porosity of MFC sheet decreased. Also enzyme beating MFC was higher value in the crystallinity and lower value in the viscosity than non-enzyme MFC. In addition, the aspect ratio of MFC was the highest at 5 pass. MFC addition improved the handsheet strength and the air permeability but worsened the drainage.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.1
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• pp.59-65
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• 2015

In this study, we evaluated optimum condition of enzyme with pH and temperature for preparation of microfibillated cellulose(MFC). Well-known endo-glucanase, three enzymes were used and CMC was used for substrate. Enzyme activity was evaluated using DNS method and absorbance with UV/VIS spectrophotometer. The enzyme shown the greatest activity was reacted with pulps at optimum condition for 1 hour and treated pulps beated until 100 mL CSF. Enzyme B and Enzyme L was the higher enzyme activity below 0.1% concentration and Enzyme N was the lowest enzyme activity. At various pH and temperature conditions, enzyme activity of Enzyme B was higher than the others at the same concentration. Especially enzyme activity at $50^{\circ}C$ of Enzyme B was almost not changed over pH 6.0. Optimum condition of three enzyme was pH 6 or pH 7 and $50^{\circ}C$ or $60^{\circ}C$. Also beating efficiency of enzyme treated pulps with Enzyme B is 55.6%.

• Journal of the Korean Graphic Arts Communication Society
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• v.19 no.1
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• pp.75-85
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• 2001

This study confirmed different results according to the molecular weight and pore properties of treated pulp. In this study the pre-treated enzyme material was increasing the beating speed compared with non-treated one. And it was observed that the change of freeness according to the combination ratio depended upon the pores size of pulp and that fibers became flexible owing to the physical deformation of fibers themselves through pre-treated enzyme increasing the flexibility of fibers to increase the contact area of fiber and thus resulting in the increase of specific surface area, the increase of fiber bonding strength and the increase of beating speed.

• Journal of the Korean Graphic Arts Communication Society
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• v.18 no.1
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• pp.121-139
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• 2000

This study is to test the possibility of applying the low-molecular weighted waste cellulase, which is produced in the process of cellulase production, to paper making. After experimenting on high-crystallized non-wood fibers with beating catalyst. I got the result that the condition for the optimal effect is temperature 40~6$0^{\circ}C$, the time 90min to 120min, pH 5.0 to 6.0, the enzyme contents 0.3% and that the effect of beating such as slight reduction of fiver viscosity, increase of water retention value(WRV) and shortening of fiber length was increased with waste cellulase. Through this process, the density, folding endurance, tensile strength and burst strength of paper was remarkably increased, which is inferred to result from the increased flexibility of fiber by individual characteristics of non-wood fiber, which was high-crystallized by penetrated low-molecular weight cellulases in the fiber.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2000.04a
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• pp.39-46
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• 2000

In pulp and papermaking process, enzymatic treatment of pulp fibres has been a topic of increasing interest in last decade. Lots of patents, papers and research reports were published on the application of enzymes in the fields of enzymatic bleaching, deinking, slime control, pitch control, waste water treatment and fibre modification. Cellulase and hemicellulase are the principal enzymes used for the modification of fibre property. This study was carried out for determinating the behaviors of enzyme to pulp fibres. A commercial enzyme, Denimax BT which is consisted with cellulase and hemicellulase, was treated to the kraft pulp produced from domestic hardwood mixtures. Results were mainly concentrated on the behaviors of freeness, drainability and fines content of fibres, and physical properties of paper with enzyme treatment. The freeness levels and dewatering ability were developed, and the fines contents were decreased. The creation of fines were controlled by the method of pre-enzyme treatment prior to fibre beating. The mechanical strength of paper, like tensile, burst, tear strength and folding endurance, were remarkably improved by the pre-enzyme treatment.

• Journal of the Korean Graphic Arts Communication Society
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• v.18 no.1
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• pp.141-155
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• 2000

To examine how the difference of molecular weight distribution of cellulase influenced the beating process according to surface pore size of the fiber, high molecular weight enzyme and low one were applied to soft wood pulp, hard wood pulp, cotton linter pulp. Some enzymes with the distribution of low molecular weight penetrated into cellulose in the proportion of surface pore size and the results were negative as like : the low viscosity, decrease of refining Yield, decrease of fine fibers content and so on. But in cotton linter pulp in the small surface area, the fiber softness was increased and it had a positive result that the paper intensity was high. Other enzymes with the distribution of high molecular weight had an enzyme reaction on the most surfaces of cellulose. They were effective in eliminating the fuzz of hydrophile fine fibers and the freeness was increased.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.3
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• pp.37-43
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• 2016

Various mechanical and chemical pretreatment methods including alkali treatment, pre-beating, enzyme treatment and oxidation treatment have been used to reduce the production energy of the microfibrillated cellulose (MFC). Among them, alkali swelling can be helpful to reduce the energy consumption because the internal bonding between fibrils could be weakened. In this study, dimethyl sulfoxide (DMSO) was used as a cosolvent to improve alkali pretreatment efficiency and the effects of NaOH concentration during NaOH-DMSO swelling on changes in fiber characteristics of softwood bleached kraft pulp (SwBKP) were elucidated. For alkali treatment in H2O-DMSO solvents, fiber length were decreased with increasing NaOH concentration while fiber width, curl and WRV were increased. WRV began to increase at 8% NaOH solution. In addition, above 8% concentration of NaOH, crystalline structure of pulp fibers converted from cellulose II to cellulose III by DMSO cosolvent. Comparing the previous results with this study, it was shown that DMSO cosolvent could promote swelling of pulp fibers and thus reduce NaOH concentration for the maximum swelling of fibers.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.6
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• pp.667-674
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• 2017

Angiotensin II (Ang II) is metabolized from N-terminal by aminopeptidases and from C-terminal by Ang converting enzyme (ACE) to generate several truncated angiotensin peptides (Angs). The truncated Angs have different biological effects but it remains unknown whether Ang-(4-8) is an active peptide. The present study was to investigate the effects of Ang-(4-8) on hemodynamics and atrial natriuretic peptide (ANP) secretion using isolated beating rat atria. Atrial stretch caused increases in atrial contractility by 60% and in ANP secretion by 70%. Ang-(4-8) (0.01, 0.1, and $1{\mu}M$) suppressed high stretch-induced ANP secretion in a dose-dependent manner. Ang-(4-8) ($0.1{\mu}M$)-induced suppression of ANP secretion was attenuated by the pretreatment with an antagonist of Ang type 1 receptor ($AT_1R$) but not by an antagonist of $AT_2R$ or $AT_4R$. Ang-(4-8)-induced suppression of ANP secretion was attenuated by the pretreatment with inhibitor of phospholipase (PLC), inositol triphosphate ($IP_3$) receptor, or nonspecific protein kinase C (PKC). The potency of Ang-(4-8) to inhibit ANP secretion was similar to Ang II. However, Ang-(4-8) $10{\mu}M$ caused an increased mean arterial pressure which was similar to that by 1 nM Ang II. Therefore, we suggest that Ang-(4-8) suppresses high stretch-induced ANP secretion through the $AT_1R$ and $PLC/IP_3/PKC$ pathway. Ang-(4-8) is a biologically active peptide which functions as an inhibition mechanism of ANP secretion and an increment of blood pressure.