• Current Research on Agriculture and Life Sciences
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• 제22권
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• pp.49-56
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• 2004

Cellulolytic enzymes were prepared from alkaline resistant microorganisms which were newly screened from calcic soil. Characteristics of enzymes and enzymatic deinking efficiency of wastepaper were investigated. The results were summarized as fellows: 1. The recovery rate of crude enzyme was 93.7% in Bio-B and 57.4% in Bio-F. 2. The protein content in crude enzymes was lowest and the thermal stability of crude enzymes was highest in Bio-F. 3. The brightness gain of Bio-F deinked pulp was best in ONP and Bio-B deinked pulp was best in MOW. 4. The reject yield was increased with enzymatic deinking flotation process. 5. The residual ink area of paper was increased with enzymatic deinking and large size of ink particles were remained in paper.

• 펄프종이기술
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• 제32권1호
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• pp.48-56
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• 2000

The objective of this study was to investigate the enzymatic deinking technology. Office recycled papers such as computer printed out(CPO) and white ledger(WL) were used in the evaluation of four different deinking conditions ; alkaline deinking , neutral deinking and enzymatic deinking at alkaline and neutral pH. Filltering pads were prepared after flotation deinking to measure brighteness gain and ink removal efficiency. Three different surfactants were also used in the evaluation of deinking efficiency including surface chemical properties by measuring foam height and stability, surface tension and cloud point of views. The results indicated that the neutral deinking method with enzyme and surfactant addition showed highest deinking efficiency of office recycled papers.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.347-350
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• 2000

Enzymatic deinking of office-waste paper was studied using crude cellulase and papain-hydrolyzed cellulase from Trichoderma reesei Rut C-30 in small-scale and mid-scale. The results were compared with deinkings using commercial enzyme(Novozym 342) and conventional chemical methods. Maximum brightness and freeness were obtained at 3 units/g Oven Dry Paper(ODP) of CMCase activity using crude cellulase in mid-scale deinking experiments. The deinked pulp had higher physical strength and brightness, and lower freeness and yield than the pulp deinked in small scale. In small scale deinking, maximum brightness and freeness were obtained at 2 unit/g ODP. Deinking by papain-hydrolyzed cellulase showed similar results with one by Novozym 342. It was better in brightness and freeness, but showed lower physical strength and yield, than the conventional deinking by sodium hydroxide. The ratio of endo-1,4-glucanase and exo-1,4-glucanase components in papain hydrolyzed cellulase from T. reesei Rut C-30 was similar to that of commercial enzyme, Novozym 342, implicating a successful application as a deinking enzyme.

• 펄프종이기술
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• 제35권1호
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• pp.34-40
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• 2003

This study was carried out to select the useful bacteria which secret extracellula enzymes for enzymatic deinking agent of old newspaper. CMCase, FPase and xylanase activities of the bacteria liquid culture were measured at optimal growth conditions. Clear zone test was checked on the solid culture. The results of this study were as follow: Eight strains of 28 bacteria isolated from a paper mill soil ground were shown strong CMCase and xylanase activity with the clear zone test. The optimal pH and temperature for culture growth were 6~8 and 26~$34^{\circ}C$, respectively and optimal culture period were less than 60 hours. Based on CMCase, FPase and xylanase activity, strain No. 18, 21, 22 and 28 which were relatively higher than the other strains, were selected for further enzymatic deinking research.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.1
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• pp.123-128
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• 2006

Enzymatic deinking method can avoids the alkaline environment as usual required in chemical deinking, which consequently cuts chemical costs and reduced the white water pollution. The electrostatic wastepaper was dinked with commercial cellulolytic enzymes and surfactant in neutral pH and the effectiveness of deinking and the physical properties of deinked pulp were evaluated. The disintegrating efficiency of the electrostatic wastepaper in neutral pH was enhanced with enzyme treatments. Although the freeness of deinked pulp with enzymes was higher than that of deinked pulp with chemical deinking agents, the brightness of the enzymatic deinked pulp was slightly lower than that of the chemical deinked pulp. But, by additions of nonionic surfactants, the brightness of deinked pulp was increased with less residual ink particles and mechanical properties of enzymatic deinked pulp was improved compared to the deinked pulp of conventional alkaline method.

• 펄프종이기술
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• 제36권3호
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• pp.9-14
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• 2004

The useful fungi which secret extracellular enzymes was selected for deinking agent of old newsprint. Five fungal strains were isolated from a paper mill soil ground. The CMCase, FPase and xylanase activities of fungi on the liquid culture were investigated at optimal growth conditions. The results of this study were as follow: The optimal pH and temperature for culture growth were 4～8 and 27～$35^{\circ}C$, respectively. For screening of extracellular enzymes at optimal culture conditions the optimal culture period were less than 6-7 days. Fusarium pallidoroseum and Aspergiilus niger which shows relatively higher CMCase, FPase and xylanase activities than the other species were selected for further enzymatic deinking research.

• 펄프종이기술
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• 제31권5호
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• pp.12-17
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• 1999

This study was to evaluate applications of the alkalophilic enzymes from Coprinus cinereus 2249 with old newsprint(ONP). A enzymatic deinking process based on alkalopholic enzymes was investigated . It was found that alkalophilic enzymes could effectively deink old newsprint. When applied on deinking of the old newsprint, it increases the freeness and brightness due to effect of hydrolysis at 0.1% enzyme concentration . Also, the physical properties of deinked pulp were improved.

• 펄프종이기술
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• 제39권5호
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• pp.12-19
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• 2007

Enzymatic deinking method can avoids the alkaline environment as usual required in chemical deinking, which consequently cuts chemical costs and reduced the white water pollution. The electrostatic wastepaper was dinked with commercial cellulolytic enzymes and surfactant in neutral pH and the effectiveness of deinking and the physical properties of deinked pulp were evaluated. The disintegrating efficiency of the electrostatic wastepaper in neutral pH was enhanced with enzyme treatments. Although the freeness of deinked pulp with enzymes was higher than that of deinked pulp with chemical de inking agents, the brightness of the enzymatic deinked pulp was slightly lower than that of the chemical deinked pulp. But, by additions of nonionic surfactants, the brightness of deinked pulp was increased with less residual ink particles and mechanical properties of enzymatic deinked pulp was improved compared to the deinked pulp of conventional alkaline method.

• 펄프종이기술
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• 제37권3호
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• pp.9-16
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• 2005

This study was carried out to investigate the characteristics of extracellular cellulase and xylanase from 4 selected different species, such as enzyme activity and stability by pH, temperature and metal ions, for application into enzymatic deinking system. The optimal temperature and pH for enzyme activity of Bacillus pumilus I, B. subtilis I, B. pumilus II and B. subtilis II were mainly $40{\sim}60^{\circ}C$ and pH $6.0{\sim}7.0$, respectively. Certain metal ions, calcium and cobalt, elevated enzyme activity, even though there were different results of enzyme activities based on various metal ions in 4 different species. With these results we suggest that enzymatic deinking system should be proceed at $50^{\circ}C$ with neutral pH condition.

• 펄프종이기술
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• 제37권4호통권112호
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• pp.1-7
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• 2005

This study was carried out to investigate the characteristics of extracellular cellulase and xylanase from Fusarium pallidoroseum and Aspergillus niger, such as enzyme activity and stability by various pH, temperature and metal ions, for application into enzymatic deinking system. The optimal temperature and pH for enzyme activity and stability of Fusarium pallidoroseum and Aspergillus niger were $50^{\circ}C$, pH 5.0 and $60^{\circ}C$, pH 9.0, respectively. Certain metal ions, calcium and cobalt, brought to elevate cellulase and xylanase activity from F. pallidoroseum and A. niger. With these results we suggest that enzymatic deinking system should be proceed at $50\~60^{\circ}C$ under their optimal pH condition.