• 한국초지조사료학회지
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• 제27권2호
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• pp.109-116
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• 2007

환경스트레스에 강한 내성을 지닌 신품종 톨페스큐를 개발할 목적으로 산화스트레스에 의해 강하게 유도되는 SWPA2 promoter 하류에 CuZnSOD와 APX 유전자가 엽록체에 동시에 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 가지며 재분화된 형질전환 식물체를 pot로 이식하여 기내 순화시킨 후, Southern 분석을 실시하여 본 결과, 발현벡터의 T-DNA 영역이 형질전환 식물체의 genome에 성공적으로 도입되었음을 확인하였다. 형질전환 식물체 잎 절편을 산화스트레스와 중금속을 포함하고 있는 용액에 처리하여 엽록체의 손상정도를 조사한 결과, 비형질 전환체에 비해 형질전환체는 강한 내성을 나타내었다. 또한 유식물체 수준에서 MV를 처리하여 내성을 비교한 결과, 비형질전환체에 비해 형질전환체는 손상을 덜 받았다. 이와 같은 연구결과는 CuZnSOD와 APX 유전자를 엽록체에 동시발현시키는 기술이 다양한 환경스트레스에 대해 복합재해내성을 가지는 다양한 작물을 개발하는데 유용하게 이용될 수 있음을 나타낸 결과이다.

• Structural Engineering and Mechanics
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• 제20권6호
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• pp.673-693
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• 2005

A general step-by-step simulation for the time-dependent analysis of segmentally-erected prestressed concrete box-girder bridges is presented. A three dimensional finite-element model for the balanced-cantilever construction of segmental bridges, including effects of the load history, material nonlinearity, creep, shrinkage, and aging of concrete and the relaxation of prestressing steel was developed using ABAQUS software. The models included three-dimensional shell elements to model the box-girder walls and Rebar elements representing the prestressing tendons. The step-by-step procedure allows simulating the construction stages, effects of time-dependent deformations of materials and changes in the structural system of the bridges. The structural responses during construction and throughout the service life were traced. A comparison of the developed computer simulation with available experimental results was conducted and good agreement was found. Deflection of the bridge deck, changes in stresses and strains and the redistribution of internal forces were calculated for different examples of bridges, built by the balanced-cantilever method, over thirty-year duration. Significant time-dependent effects on the bridge deflections and redistribution of internal forces and stresses were observed. The ultimate load carrying capacities of the bridges and the behavior before collapse were also determined. It was observed that the ultimate load carrying capacity of such bridges decreases with time as a result of time-dependent effects.

• Computers and Concrete
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• 제14권5호
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• pp.613-633
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• 2014

A double-skinned composite tubular (DSCT) column, which consists of concrete and inner and outer tubes, was finally developed to overcome the weaknesses of concrete filled tube columns by reducing the self-weight of the column and confining the concrete triaxially. Research pertaining to the stiffness and strength of the column and the confining effect in a DSCT column has been carried out. However, detailed studies on the confining stress, especially the internal confining stress in a DSCT column, have not been carried out. Internal and external confining stresses should be evaluated to determine the effective confining stress in a DSCT column. In this paper, the confining stresses of concrete before and after insertion of an inner tube were studied using finite element analysis. The relationship between the internal or external confining stresses and the theoretical confining stress was investigated by parametric studies. New modified formulae for the yield and buckling failure conditions based on the formulae suggested by former researchers were proposed. Through analytical studies, the modified formulae were verified to be effective for economic and reasonable design of the inner tubes in a DSCT column under the same confining stress.

• 한국수자원학회논문집
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• 제49권8호
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• pp.707-717
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• 2016

본 연구에서는 직선 개수로 내에 설치된 수제 모형 주위에서 발생하는 난류 흐름의 특성을 알아보기 위하여 수리모형실험을 수행하였다. 실험은 Froude 수가 0.100과 0.185인 두 가지 흐름 조건에서 이루어졌다. 시간평균유속과 난류 응력을 구하기 위하여 초음파 유속계를 이용하여 3차원 순간 유속을 측정하였다. 실험 결과, 수제 하류에서 난류 응력이 큰 재순환 영역이 존재하는 것으로 확인하였다. Froude 수가 큰 경우와 작은 경우의 전반적인 평균 유속 분포는 유사하게 나타났으나, 최대 무차원 난류 응력과 최대 무차원 바닥 전단응력은 Froude 수가 증가할수록 차이를 보였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.465-465
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• 2005

• Structural Engineering and Mechanics
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• 제31권6호
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• pp.751-754
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• 2009

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.116-116
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• 2002

It is believed that cell and/or tissue toxicity is resulted from alterations in expression of many genes in response to environmental stresses or toxicants. New technology, such as DNA microarray analysis, can measure the expression of thousands of genes at a time providing the potential to accelerate discovery of toxicant pathways and specific gene targets.(omitted)

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.66.1-66
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• 2002

• Smart Structures and Systems
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• 제7권4호
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• pp.275-287
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• 2011

The behaviors of saturated soils such as compressibility and permeability are distinguished by preconsolidation stress. Preconsolidation stress becomes an important design parameter in geotechnical structures. The goal of this study is to introduce a new method for the evaluation of preconsolidation stress based on the shear wave velocity at small strain, using Busan, Incheon, and Gwangyang clays in Korea. Standard consolidation tests are conducted by using an oedometer cell equipped with bender elements. The preconsolidation stresses estimated by shear wave velocity are compared with those evaluated by the Casagrande, constrained modulus, work, and logarithmic methods. The preconsolidation stresses estimated by the shear wave velocity produce very similar values to those evaluated by the Onitsuka method (one of the logarithmic methods), which yields an almost real preconsolidation stress. This study shows that the shear wave velocity method provides a reliable method for evaluating preconsolidation stress and can be used as a complementary method.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.422-422
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• 2005