• Journal of Ginseng Research
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• 제34권4호
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• pp.296-303
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• 2010

A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type II peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RT-PCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

• Smart Structures and Systems
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• 제17권6호
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• pp.995-1015
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• 2016

In the design and condition assessment of bridges, it is usually necessary to take into consideration the extreme conditions which are not expected to occur within a short time period and thus require an extrapolation from observations of limited duration. Long-term structural health monitoring (SHM) provides a rich database to evaluate the extreme conditions. This paper focuses on the extrapolation of extreme traffic load effects on bridges using long-term monitoring data of structural strain. The suspension Tsing Ma Bridge (TMB), which carries both highway and railway traffic and is instrumented with a long-term SHM system, is taken as a testbed for the present study. Two popular extreme value extrapolation methods: the block maxima approach and the peaks-over-threshold approach, are employed to extrapolate the extreme stresses induced by highway traffic and railway traffic, respectively. Characteristic values of the extreme stresses with a return period of 120 years (the design life of the bridge) obtained by the two methods are compared. It is found that the extrapolated extreme stresses are robust to the extrapolation technique. It may owe to the richness and good quality of the long-term strain data acquired. These characteristic extremes are also compared with the design values and found to be much smaller than the design values, indicating conservative design values of traffic loading and a safe traffic-loading condition of the bridge. The results of this study can be used as a reference for the design and condition assessment of similar bridges carrying heavy traffic, analogous to the TMB.

• 통합자연과학논문집
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• 제12권4호
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• pp.127-132
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• 2019

Programmed cell death 4 (PDCD4) is a novel tumor suppressor that function in the nucleus and the cytoplasm and appears to be involved in the regulation of transcription and translation. Stress granules (SGs) are cytoplasmic foci at which untranslated mRNAs accumulate when cells exposed to environmental stresses. Since PDCD4 has implicated in translation repression through direct interaction with eukaryotic translation initiation factor 4A (eIF4A), we here investigated if PDCD4 has a functional role in the process of SG assembly under oxidative stresses. Using immunofluorescence microscopy, we found that PDCD4 is localized to SGs under oxidative stresses. Next, we tested if knockdown of PDCD4 has an effect on the assembly of SG using PDCD4-specific siRNA. Interestingly, SG assembly was accelerated and this effect was caused by sensitization of phosphorylation of eIF2α and dephosphorylation of eIF4E binding protein (4E-BP). These results suggest that PDCD4 has an effect on SG dynamics and possibly involved in cap-dependent translation repression under stress conditions.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.175-181
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• 2012

Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184 (Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184 fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.130-136
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• 2011

Ions deficiency or excess remains one of the critical ground level environmental problems, affecting crop productivity. In this overview, we will discuss an increased application of proteomics technology in addressing this issue using rice (Oryza sativa L.) as a model crop plant. Proteomics analyses have revealed that rice proteome undergoes changes in the proteins composition and expression in response to several ionic stresses, including mineral nutrients (aluminum, nitrogen, and phosphorous) and heavy metals (arsenic, cadmium, and copper). Developed inventory of responsive proteins and their correlation with changes in physiological symptoms and parameters are a major step forward in: (i) better understanding the underlying mechanisms of ionic stresses-triggered responses in rice; (ii) comparative proteomics studies; and (iii) designing a novel strategy to improve crop plants.

• 한국재난정보학회 논문집
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• 제13권2호
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• pp.147-154
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• 2017

듀플렉스 스테인리스강은 일반 오스테나이트계 스테인리스강과는 다른 열적, 기계적 성질로 인하여 일반 스테인리스강과는 다른 잔류응력 크기와 분포를 가진다. 따라서 듀플렉스 스테인리스강을 강구조물에 적용시키기 위해서는 용접부의 잔류응력 특성에 대한 연구가 반드시 선행되어야 한다. 본 연구에서는 듀플렉스 스테인리스강 용접시 발생하는 잔류응력을 스트레인 게이지를 이용한 응력 이완법을 이용하여 실험적으로 측정하여 용접부의 잔류응력 특징을 조사하였다.

• Structural Engineering and Mechanics
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• 제36권4호
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• pp.401-419
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• 2010

This paper focuses on the development, verification and application of a three-dimensional nite element code for coupled thermal and structural analysis of roller compacted concrete arch dams. The Ostour Arch dam located on Ghezel-Ozan River, Iran, which was originally designed as conventional concrete arch dam, has been taken for the purpose of verication of the nite element code. In this project, RCC technology has been ascertained as an alternative method to reduce the cost of the project and make it competitive. The thermal analysis has been carried out taking into account the simulation of the sequence of construction, environmental temperature changes, and the wind speed. In addition, the variation of elastic modulus with time has been considered in this investigation using Concard's model. An attempt was made to compare the stresses developed in the dam body five years after the completion of the dam with those of end of the construction. It was seen that there is an increase in the tensile stresses after five years over stresses obtained immediately at the end of construction by 61.3%.

• 대한토목학회논문집
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• 제28권6A호
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• pp.881-891
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• 2008

국내 최초로 시공되는 롤러다짐 콘크리트 댐(RCD)의 온도균열 관리방안 수립하기 위하여 RCD 공법의 시공특성인 층(layer) 타설 방식을 고려하여, 댐체의 온도분포 및 온도응력 해석을 수행하였다. RCD 공법은 수 백 개의 시공단계로 구성되어 있고, 실제 타설 층을 시공단계로 해석하는 것과 6층을 한 시공단계로 시공하는 경우를 비교하여 해석 단계의 단순화 가능성을 검토하였으며, 단위시멘트량이 $130kg/m^3$ 내외로 발열량이 매우 작은 RCD 배합의 경우에도 여름철에는 온도균열 지수가 1.0 이하로 나타나 온도관리의 필요성을 확인하였다.

• Steel and Composite Structures
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• 제34권3호
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• pp.423-440
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• 2020

In the recent years, steel box girder bridges have been extensively used due to high bending stiffness, torsional rigidity, and rapid construction. Therefore, researches related to this girder bridge have been widely conducted. This paper investigates the effect of residual stresses and geometric imperfections on the load-carrying capacity of steel box girder bridges spanning 30 m and 50 m. A three - dimensional finite element model of the steel box girder with a closed section was developed and analyzed using ABAQUS software. Nonlinear inelastic analysis was used to capture the actual response of the girder bridge accurately. Based on the results of analyses, the superimposed mode of webs and flanges was recommended for considering the influence of initial geometric imperfections of the steel box model. In addition, 4% and 16% strength reduction rates on the load - carrying capacity of the perfect structural system were respectively recommended for the girders with compact and non-compact sections, whose designs satisfy the requirements specified in AASHTO LRFD standard. As a consequence, the research results would help designers eliminate the complexity in modeling residual stresses and geometric imperfections when designing the steel box girder bridge.

• 한국초지조사료학회지
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• 제38권3호
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• pp.170-174
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• 2018

Environmental stresses caused by climate change, such as high temperature, drought and salinity severely impact plant growth and productivity. Among these factors, high temperature stress will become more severe during summer. In this study, we examined physiological and molecular responses of maize plants to high temperature stress during summer. Highest level of $H_2O_2$ was observed in maize leaves collected July 26 compared with June 25 and July 12. Results indicated that high temperature stress triggers production of reactive oxygen species (ROS) in maize leaves. In addition, photosynthetic efficiency (Fv/Fm) sharply decreased in leaves with increasing air temperatures during the day in the field. RT-PCR analysis of maize plants exposed to high temperatures of during the day in field revealed increased accumulation of mitochondrial and chloroplastic small heat shock protein (HSP) transcripts. Results demonstrate that Fv/Fm values and organelle-localized small HSP gene could be used as physiological and molecular indicators of plants impacted by environmental stresses.