• Title/Summary/Keyword: environmental DNA

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Chromium-Induced Cytotoxicity in CHO Cells (크롬에 의한 CHO 세포의 세포독성기전에 관한 연구)

  • 기혜성;손은희;유일재;맹승희;정해원
    • Journal of Environmental Health Sciences
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    • v.22 no.4
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    • pp.77-81
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    • 1996

  • The present experiment was carried out to examine the mechanism of cytotoxicity of Chromium in CHO cells. Chromium induced chromosomal aberrations in a dose-dependent manner. The most frequent type of aberration was chromatid deletions and chromosome type exchanges were also observed. Ultrafiltrates of culture media from CHO cells treated with Chromium induced sister chromatid exchanges(SCE) in CHO cells and Chromium induced lipid peroxidation. It was suggested that indirect effect through formation of clastogenic factor(CF) as well as direct effect on DNA might contribute to the cytotoxicity of Chromium.

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Comparison of Sensitivity of Detetion for Clostridium perfringens Type A Enterotoxin by the Reversed Passive Latex Agglutination and the Polymerase Chain Reaction

  • 정희곤
    • Journal of Environmental Health Sciences
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    • v.23 no.4
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    • pp.45-49
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    • 1997

  • Clostridium perfringens A형이 생산하는 장독소를 검색해본 결과, RPLA법에 있어서는 2배로 희석한 용액으로부터 64배로 희석한 용액 (NCTC 8239 Hobbs serotype 3 CPE$^+$)에서까지 양성반응을 보였으며 PCR 기법에 있어서는 10 pg 희석 용액까지 364 bp의 장독소 DNA fragment(NCTC 8238 Hobbs serotype 2 CPE$^+$)를 확인 할수 있었다. 그러므로 장독소를 검색하기 위해서는 PCR기법이 RPLA법에 비하여 훨씬 감도가 높음을 확인할 수 있었다.

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Reduction of Dioxin-Induced Expression of cyplal Gene through Repression of AhR/Arnt DNA Binding by Mek-1 inhibitor PD98059

  • Park, Hyunsung
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.60-66
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    • 2002

  • Aryl hydrocarbons, environmental contaminants accumulate in tissue and pose potential risk in human health. 2,3,7,8-Tertachlorodibenzo-p-dioxin (TCDD) is known as a most potent toxicant among aryl hydrocarbons. TCDD elicits numerous toxic responses in experimental animals and human, including hepatic carcinoma, pulmonary and skin tumor in adult rodents, craniofacial abnormality during mouse embryogenesis, chloracne, reproductive abnormality, immunotoxicity, endocrine effects in exposed humans.(omitted)

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