• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.107-107
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• 2019

Ulcerative colitis (UC) is one of inflammatory bowel disease (IBD), characterized by chronic inflammatory response and dysregulation of immune function. The severity of US has been influenced by environmental factors and food habit. The immune modulatory, anti-inflammatory and steroidal medicine have been used for the treatment of UC. However, long-term administration of those medicine is accompanied with side-effect. So, it is necessary to develop the non side-effect medicine using natural product. Prebiotics influences intestinal condition and food consumption. The heredity, immunity and environmental condition are related with occurrence of UC. In recent study, UC patients had lower level of prebiotics such as Lactobacillus and Bifidobacterium compared with healthy people. Also, previous study announced that imbalance of enteric flora aggravates the severity of UC. The effectiveness of probiotics might affect colon ability and viable bacteria also could promote the proliferation of beneficial intestinal bacteria. Prebiotics, such as herbal medicine, could lead to balance of intestinal bacteria or increase beneficial bacteria. So, proper choice of herbal medicine could control the intestinal condition. This study aimed to investigate the effect of mixture of probiotics and prebiotics (synbiotics) on dextran sulfate sodium (DSS)-induced UC in vivo. The synbiotics consist of Lactobacillus buchneri, Polymnia sonchifolia and Glycine max Merr. in this study. To evaluate the effect of synbiotics, 3% DSS was administered in BALB/c mice and synbiotics was daily administered for experimental days. The administration of synbiotics regulated colon length shortening, body weight change and disease activity index effectively. Also, extract of synbiotics upregulated survival ability of Lactobacillus buchneri in gut condition. These results suggest that mixture of probiotics and prebiotics, called as synbiotics, could influence intestinal condition also regulate the colon disease. Synbiotics might be a therapeutic agent for treatment of UC.

• 한국가금학회지
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• 제38권2호
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• pp.155-164
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• 2011

The use of antimicrobials will be soon removed due to an increase of occurrence of antibiotic-resistant bacteria or ionophore-resistant Eimeria species in poultry farms and consumers' preference on drug-free chicken meats or eggs. Although dietary antimicrobials contributed to the growth and health of the chickens, we do not fully understand their interrelationship among antimicrobials, gut microbiota, and host immunity in poultry. In this review, we explored the current understanding on the effects of antimicrobials on gut microbiota and immune systems of chickens. Based on the published literatures, it is clear that antibiotics and antibiotic ionophores, when used singly or in combination could influence gut microbiota. However, antimicrobial effect on gut microbiota varied depending on the samples (e.g., gut locations, digesta vs. mucosa) used and among the experiments. It was noted that the digesta vs. the mucosa is the preferred sample with the results of no change, increase, or decrease in gut microbiota community. In future, the mucosa-associated bacteria should be targeted as they are known to closely interact with the host immune system and pathogen control. Although limited, dietary antimicrobials are known to modulate humoral and cell-mediated immunities. Ironically, the evidence is increasing that dietary antimicrobials may play an important role in triggering enteric disease such as gangrenous dermatitis, a devastating disease in poultry industry. Future work should be done to unravel our understanding on the complex interaction of host-pathogen-microbiota-antimicrobials in poultry.

• 미생물학회지
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• 제20권1호
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• pp.21-26
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• 1982

The disinfection effects of sunlight and predacious microorganisms on enteria of Escherichia coli and Proteus vulgaris were examined in sewage water at 22 to $26^{\circ}C$. The test prganisms were disinfected by 4 to 5 logs during 6-hour a day exposure to sunlight for seven days. The effect was proportional to the light intensity and was proved to be dur to the action of the visible light of short wavelength which can transmit the soft glass of a flask glass. The predacious microorganisms decreased trhe test organisms by about 3 to 4 logs during 7 days. The complex actions of sunlight and predacious microorganisms were variable depending upon the test organisms, but the total effects of those enviromental factors were yet completely understood.

• 미생물과산업
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• 제12권2호
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• pp.14-18
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• 1986

Barcterial chemotaxis is a transient response of an organism in a situation where environmental homogeneity has been disturbed by certain chemical compounds. The phenomenon has been described in motile bacterial species including enteric bacteria, Gram-positives(14), Spirochaetes (6) and even Archaebacteria (8). However, most comprehensive studies have been done with Escherichia coli and Salmonella typhimurium. Two analogies to higher eucaryotic sensory phenomena are provided by the study of bacterial chemotaxis. First, bacterial chemotaxis is similar to the stimulus-response of neuronal, immune and sperm cells. Second, studies of individual components involved in the bacterial sensory pathway can contribute to the understanding of the function of receptors, controling signals and molecular comparators in transmembrane signalling system. The bacterial sensory transducer, a chemoreceptor in a broad sense, is a unique entity for studying sensory function in which sensory reception, signalling and adaptation are integrated (7,18).

• 대한미생물학회지
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• 제16권1호
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• pp.13-18
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• 1981

Total 984 specimens were collected from sweages(130 specimens) and human rectal swabs(854 specimens) during period from October 1979 to November 1980 in Korea. Sixteen strains of salmonella were isolated from the human rectal swabs at the St. Mary's Hospital in Dae Jeon. They were confirmed into fifteen strains of S. typhi, one strain of S. paratyphi A. In other hand, fourty three strains of Shigella had been isolated from the stool specimens. They were typed as thirty four strains of Shigella flexneri, five strains of Shigella sonnei and one strain of Shigella dysenteriae. However, 16 strains of Vibrio eltor had been isolated from patients and carriers in the first epidemic areas in Chunla Namdo in September 1980. The serotype of isolated strains was determined as a Inaba which was resistant to the Mukerjee phage group IV but was susceptible to El Tor phage groupe I and II. Authors had been concluded that the strains were certain to be identified with Vibrio cholerae biotype El Tor.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2000년도 학술대회지
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• pp.18-18
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• 2000

• 약학회지
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• 제12권3_4호
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• pp.72-75
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• 1968

It has been believed that "Kup kee" (Chloranthus japonica) is good for boils, or dermatopathia, and enteric fever by the Korean folks. However, there is no scientific report on its chemical ingredients and its antibacterial activities so far. The roots of Chloranthus japonica were dried and pulverized. The pulverized powder was then extracted with methanol. Then an extract was obtained by following Stas Otto's method. finally a crystal fraction was obtained through Column Chromatography. Recrystalization from methanol gave white needle crystals, m.p. $198^{\circ}C.$ Generally this compound has shown strong antibacterial effects on Gram negative bacteria. It exerts most powerful effect on Salmonella typhi. on the other hand it shows weak effect on Inaba cholera.

• 한국자연치유학회지
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• 제8권1호
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• pp.1-10
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• 2019

목적: 본 연구는 임상대상자 30명에게 미생물발효용액인 Zen발효용액을 8주간 섭취시킨 후에 임상대상자들의 변에서 중요한 장내 유익세균 및 저해세균의 증식이 촉진되는지 또는 감소하는지를 연구하는 것이 목적이었다. 방법: 장내세균은 특정 Primer를 이용하여 PCR 증폭기로 동정 검색하였다. 결과: Zen발효액을 섭취한 임상군의 Bifidobacterium genus 유전자복제지수(gi%)는 섭취 전 수치는 55.15%, 섭취 후에는 70.1%로 나타났으며, 섭취 후에 14.95% 차이로 유의성이 있게 증가하였다. 아래 모든 대조군은 유의성이 없었다. 임상군의 Lactobacillus genus지수는 섭취 전이 46.87%, 섭취 후가 60.91%로 나타났으며, 섭취 후에 14.04% 차이로 유의성은 있게 증가하였다(p<.01). 임상군의 Clostridium genus지수는 사전이 85.64%, 사후가 65.99%로 나타났으며, 섭취 후에 -19.65% 차이로 유의성이 있게 감소를 하였다(p<.017). 임상군의 Bacteroides genus지수는 사전이 17.11%, 사후가 20.22%로 나타났으며, 섭취 후에 3.11% 차이로 유의성이 있게 증가하였다. 임상군의 Prevotella genus지수는 사전이 14.01%, 사후가 16.79%로 나타났으며, 섭취 후에 2.78%차이로 증가하였으나 유의성은 없었다. 결론: 장내세균은 혼합미생물의 발효액 Zen을 섭취 후에 장내에서 유익균은 증식이 증가하고, 유해균은 억제되는 현상을 발견하였다. Zen발효액은 장 건강에 유익한 음료라 평가한다.

• 미생물학회지
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• 제11권4호
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• pp.167-174
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• 1973

This study included two parts of investigation, the microfloral changes during the brewing process with the changes of pH, total acidity, temperature and alcoholic contents, as well as determination of survival times of major enteric pathogens in Takju. 1. Maximum number of Saccharomyces cerevisiae was $4.3{\times}10^7$ per milliliter on the 5th day of fermentation and gradually decreased. Saccharomyces cerevisiae was one of the predominant strains of the fermentation process. The number of Saccharomyces cerevisiae was $4.3{\times}10^6$ per milliliter at the completion of the brewing and human consumption. In a few days after the completion of the brewing. Bacillus subtilis and some species of Staphylococcus spp. began to grow and those organisms were responsible for the spoilage. 2. Maximum pH, during the brewing, was 5.8 on the first day of fermentation and rapidly decreased until 6th day of fermentation at pH 4.3. 3. Maximum alcholic content was 14.5 degree on the 4th day of fermentation, 10.3 degree on the 5th day and this degree was continued during the experimentation. 4. Maximum temperature, during Takju brewing was 34.deg.C on the 3rd day of fermentation and rapidly decreased up to 23.deg.C on the 6th day and this temperature was continued until the brewing process was finished. 5. Maximum total acidity was 0.57 percent on the 4th day of fermentation and gradually decreased by brewing process was completed. 6. Survival time of major enteric pathogenic bacteria in Takju was as follows : Shigella dysenteriae and Escherichia coli were isolated in two hours and 14 hours respectively, but Salmonella typhi, Vibrio parahemolyticus were not isolated even in an hour after the inoculation of those organisms in undiluted Takju. In diluted Takju, Salmonella typhi, Vibrio parahemolyticus were not isolated even in an hour after the inoculation of those organisms in undiluted Takju. In diluted Takju, Salmonella typhi, Shigella dysenteriae, and Escherichia coli were survived for 50-60 hours, but Vibrio cholerae and Vibrio parahemolyticus were not isolated even if treated within one hour.

• 대한수의학회지
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• 제39권1호
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• pp.118-125
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• 1999

Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.