• 한국식품과학회지
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• 제43권5호
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• pp.618-623
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• 2011

E. coli O157:H7은 요독증후군, 출혈성 대장염 및 설사 등을 유발하는 원인균으로, 전세계적으로 이들의 오염에 따른 식중독 발생이 증가하고 있어, 본 연구에서는 E. coli O157:H7을 신속 정확하게 분석할 수 있는 면역크로마토그래피법을 개발하였고, 보다 신속한 검출을 위하여 육류와 새싹시료를 대상으로 최소 증균시간을 확인하였다. 먼저, 면역크로마토그래피법의 개발을 위해 colloidal gold와 EC MAb를 합성하여 EC MAb-gold conjugate를 제작한 다음, 접합여부를 확인한 결과, test line과 control line에 각각 처리되는 EC MAb와 anti-mouse IgG에 특이적으로 반응하였다. 제작된 EC MAb-gold conjugate를 이용하여 개발된 면역크로마토그래피법의 검출한계는 $1{\times}10^5$ CFU/mL 수준까지 검출이 가능한 것으로 확인되었고, 다른 대장균속 및 주요 식중독균과의 교차반응성은 나타나지 않았다. 개발된 면역크로마토그래피법을 이용하여 임의로 E. coli O157:H7을 오염시킨 돼지고기, 소고기, 닭고기 및 새싹채소를 증균하는 동안 분석한 결과, 돼지고기와 닭고기는 6시간, 소고기는 10시간, 특히 새싹채소는 2시간 후부터 $1{\times}10$ CFU/100 ${\mu}L$ 수준까지 검출이 가능한 것으로 나타났다. 따라서 본 연구에서 개발된 면역크로마토그래피법을 이용하여 육류와 채소 등에 오염된 E. coli O157:H7을 최대 10시간 이내로 신속히 분석할 수 있을 것으로 판단된다.

• 한국조리학회지
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• 제24권3호
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• pp.188-195
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• 2018

The objective of this study was to evaluate the quality characteristics of moringa powder enrichment in sponge cake. The developed product can be a promising inclusion in the functional foods. Moringa powder was added to sponge cake batter (in the ratios of 0, 3, 6, 9, 12% of the total flour), called MP3, MP6, MP9, and MP12 respectively. The effect of moringa supplementation on physical, chemical and sensory quality of cakes were evaluated and analyzed statistically using SPSS program. The pH of cake was decreased as the concentration of moringa powder added. The specific volume of cake samples decreased with increasing levels of moringa powder in the formulation. Colour measurements showed that lightness and yellowness were decreased with an increased level of moringa powder. But redness result revealed and increasing in redeness with the increasing morigna powder level, compared to the control sample. The results of texture profile analysis showed hardness, springness, cohesiveness of the moringa cake that were higher than control sample. In sensory evaluation, MP3 was selected as the most acceptable sample. In the conclusion, moringa powder was incorporated in the formulation to enrich sponge cakes and was suggested for development of functional foods.

• 대한수학교육학회지:학교수학
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• 제11권2호
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• pp.207-225
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• 2009

수학영재의 지적 욕구를 충족시키고 창의성을 신장시키는 문제해결 중심의 수업활동을 하기 위해서는 수학영재의 수준에 맞게 만들어진 문제가 필수적이다. 본 논문의 목적은 수학영재 지도교사의 교수 능력을 신장시키기 위한 심화 연수 과정의 일부인 원격 연수에 참여한 수학영재 지도교사가 만든 문제의 형태를 문제 접근 방법에 따라 '익숙한 문제', '익숙하지 않은 문제', '오류가 있는 문제'로 나누어 분석하여 수학영재 지도교사를 위한 원격 연수에서 교사의 문제만들기에 대한 실천적 방안을 제시하는데 있다.

• 생명과학회지
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• 제21권5호
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• pp.700-705
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• 2011

농화배양법을 이용하여 울산공단 일대의 폐수 및 토양에서 유기용매 내성 Bacillus sp. BCNU 5005를 분리하였다. 16S 리보좀DNA 염기서열 분석결과 BCNU 5005 균주는 B. subtilis와 98% 상동성을 가진 것으로 나타났으며 계통학적으로도 B. subtilis임이 확인되었다. 일반적으로 대부분의 세균과 그들의 효소는 고농도 유기용매하에서 불활성화되거나 파괴된다. 그러나. Bacillus sp. BCNU 5005의 lipase 활성은 chloroform, ethylbenzene 그리고 decane을 제외한 다양한 종류의 유기용매(25%, v/v)에서 매우 안정함을 보였다. 게다가 BCNU 5005는 유기용매를 분해하는 능력을 가진 것으로 확인하였다. 유기용매 내성 Bacillus sp. BCNU 5005는 생물전환과 생물복구산업을 위한 새로운 잠재적인 자원으로서 이용될 수 있다.

• Child Health Nursing Research
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• 제10권1호
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• pp.89-97
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• 2004

Purpose: Adolescence is viewed as a critical period in the formation of health behavior and many health behaviors developed during this era persist into adulthood. Social-cognitive theory, self-efficacy as a central construct, has been used to predict and intervene the health behavioral patterns in adolescent. Previous research demonstrated that the attachment in childhood and self-esteem as psychosocial factors are predictors of the health efficacy in adolescent and it is viewed as solely an antecedent for the lifelong health behavior. Method: To investigate the path pattern of attachment in childhood, self-esteem and health efficacy in adolescents, 381 adolescents in high school from two urban cities in Korea were recruited for cross-sectional sample. Attachment in childhood was measured using Mother-Father-Peer Scale. Self esteem was measured with Hare self esteem. Perceived self efficacy in Health was measured by the School Health Efficacy Questionnaire. The path analysis revealed a significant relationship between attachment in childhood and self-esteem, self-esteem and health efficacy in adolescents. Self esteem was the strongest contributing factor for health efficacy in adolescent. The results suggest that attachment in childhood may aid in formulating positive self -esteem in adolescents and self-esteem played a major role in predicting health efficacy in adolescents. Conclusion: Therefore, self-esteem enrichment should be incorporated with adolescent health promotion and certainly should be a component in any health education program in school health program and interventions. These results have implications of psychosocial and family related factors on health promotion and health education for the health care provider with regard to primary and secondary prevention in adolescent population.

• 한국문헌정보학회지
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• 제48권4호
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• pp.51-70
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• 2014

연구의 주요 목적은 정보활용능력과 수사학의 융합을 통해 정보활용능력 교육이 대학에서 필수적인 교양과목의 요건을 갖출 수 있는지를 논증하는 것이다. 연구 결과 수사학적 정보활용능력의 성격을 1) 발견법을 이용한 상황적 활용능력, 2) 탐구를 중시하는 과정 중심 활용능력, 3) 편재성에 적합한 활용능력으로 정리하였다. 이렇게 하여 수사학적 정보활용능력이 학제성을 띠는 민주시민의 핵심 교양이 될 수 있음을 논증하였다. 정보활용능력의 교육에는 기술 이용 중심의 방법도 있는 등 다양성이 존재하는 만큼 이 부분과의 비교가 필요하다. 수사학적 정보활용능력 교육의 실제 프로그램으로는 '문제 발견을 위한 상황적 질문하기', '학습론 책 선별해서 읽고 서평 쓰기', '실제 도서관 탐방 보고서 쓰기', '민주시민을 위한 정보윤리 교육하기' 등을 제시하였다.

• Genomics & Informatics
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• 제13권4호
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• pp.137-145
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• 2015

Selective sweep can cause genetic differentiation across populations, which allows for the identification of possible causative regions/genes underlying important traits. The pig has experienced a long history of allele frequency changes through artificial selection in the domestication process. We obtained an average of 329,482,871 sequence reads for 24 pigs from three pig breeds: Yorkshire (n = 5), Landrace (n = 13), and Duroc (n = 6). An average read depth of 11.7 was obtained using whole-genome resequencing on an Illumina HiSeq2000 platform. In this study, cross-population extended haplotype homozygosity and cross-population composite likelihood ratio tests were implemented to detect genes experiencing positive selection for the genome-wide resequencing data generated from three commercial pig breeds. In our results, 26, 7, and 14 genes from Yorkshire, Landrace, and Duroc, respectively were detected by two kinds of statistical tests. Significant evidence for positive selection was identified on genes ST6GALNAC2 and EPHX1 in Yorkshire, PARK2 in Landrace, and BMP6, SLA-DQA1, and PRKG1 in Duroc. These genes are reportedly relevant to lactation, reproduction, meat quality, and growth traits. To understand how these single nucleotide polymorphisms (SNPs) related positive selection affect protein function, we analyzed the effect of non-synonymous SNPs. Three SNPs (rs324509622, rs80931851, and rs80937718) in the SLA-DQA1 gene were significant in the enrichment tests, indicating strong evidence for positive selection in Duroc. Our analyses identified genes under positive selection for lactation, reproduction, and meat-quality and growth traits in Yorkshire, Landrace, and Duroc, respectively.

• Animal cells and systems
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• 제16권1호
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• pp.50-56
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• 2012

Kelp grouper ($Epinephelus$ $bruneus$ Bloch 1793) is a commercially important fish in Korea. In recent years, the catch of kelp grouper in the coastal waters of Korea has significantly declined. Despite its importance, little is known about its genetic diversity and conservation efforts are hampered. In this study, we isolated and characterized 12 microsatellite loci using an enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. All loci were readily amplified and contained TG/CA denucleotide repeats. To characterize each locus, 30 individuals from a natural E. bruneus population in the coastal waters of Jeju Island, Korea, were genotyped. All loci except three, KEm118, KEm154, and KEm219, were polymorphic, with an average of 8.1 alleles per locus (range 2-18). The mean observed and expected heterozygosities were 0.47 (range 0.19-1.00) and 0.61 (range 0.29-0.92), respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at three loci (KEm134, KEm184, and KEm283). These findings will be useful for effective monitoring and management of genetic variation of kelp grouper as well as for the implementation of a fisheries conservation program.

• Journal of Radiation Protection and Research
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• 제42권1호
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• pp.48-55
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• 2017

Background: Simultaneous detection of neutrons and gamma rays have become much more practicable, by taking advantage of good gamma-ray discrimination properties using pulse shape discrimination (PSD) technique. Recently, we introduced a commercial CLYC system in Korea, and performed an initial characterization and simulation studies for the CLYC detector system to provide references for the future implementation of the dual-mode scintillator system in various studies and applications. Materials and Methods: We evaluated a CLYC detector with 95% $^6Li$ enrichment using various gamma-ray sources and a $^{252}Cf$ neutron source, with validation of our Monte Carlo simulation results via measurement experiments. Absolute full-energy peak efficiency values were calculated for gamma-ray sources and neutron source using MCNP6 and compared with measurement experiments of the calibration sources. In addition, behavioral characteristics of neutrons were validated by comparing simulations and experiments on neutron moderation with various polyethylene (PE) moderator thicknesses. Results and Discussion: Both results showed good agreements in overall characteristics of the gamma and neutron detection efficiencies, with consistent ~20% discrepancy. Furthermore, moderation of neutrons emitted from $^{252}Cf$ showed similarities between the simulation and the experiment, in terms of their relative ratios depending on the thickness of the PE moderator. Conclusion: A CLYC detector system was characterized for its energy resolution and detection efficiency, and Monte Carlo simulations on the detector system was validated experimentally. Validation of the simulation results in overall trend of the CLYC detector behavior will provide the fundamental basis and validity of follow-up Monte Carlo simulation studies for the development of our dual-particle imager using a rotational modulation collimator.

• Genomics & Informatics
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• 제18권4호
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• pp.42.1-42.9
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• 2020

Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-Seq) is a powerful technology to profile the location of proteins of interest on a whole-genome scale. To identify the enrichment location of proteins, many programs and algorithms have been proposed. However, none of the commonly used peak calling programs could accurately explain the binding features of target proteins detected by ChIP-Seq. Here, publicly available data on 12 histone modifications, including H3K4ac/me1/me2/me3, H3K9ac/me3, H3K27ac/me3, H3K36me3, H3K56ac, and H3K79me1/me2, generated from a human embryonic stem cell line (H1), were profiled with five peak callers (CisGenome, MACS1, MACS2, PeakSeq, and SISSRs). The performance of the peak calling programs was compared in terms of reproducibility between replicates, examination of enriched regions to variable sequencing depths, the specificity-to-noise signal, and sensitivity of peak prediction. There were no major differences among peak callers when analyzing point source histone modifications. The peak calling results from histone modifications with low fidelity, such as H3K4ac, H3K56ac, and H3K79me1/me2, showed low performance in all parameters, which indicates that their peak positions might not be located accurately. Our comparative results could provide a helpful guide to choose a suitable peak calling program for specific histone modifications.