• Title/Summary/Keyword: enrichment

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Microarray Analysis of Genes Involved with Shell Strength in Layer Shell Gland at the Early Stage of Active Calcification

  • Liu, Zhangguo;Zheng, Qi;Zhang, Xueyu;Lu, Lizhi
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.5
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    • pp.609-624
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    • 2013
  • The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of purebred Xianju hens with consistent high or low shell breakage strength were grouped into two pairs. Using Affymetrix Chicken Array, a whole-transcriptome analysis was performed on hen's shell gland at 9 h post oviposition. Gene ontology enrichment analysis for differentially expressed (DE) transcripts was performed using the web-based GOEAST, and the validation of DE-transcripts was tested by qRT-PCR. 1,195 DE-transcripts, corresponding to 941 unique genes were identified in hens with strong eggshell compared to weak shell hens. According to gene ontology annotations, there are 77 DE-transcripts encoding ion transporters and secreted extracellular matrix proteins, and at least 26 DE-transcripts related to carbohydrate metabolism or post-translation glycosylation modification; furthermore, there are 88 signaling DE-transcripts. GO term enrichment analysis suggests that some DE-transcripts mediate reproductive hormones or neurotransmitters to affect eggshell quality through a complex suite of biophysical processes. These results reveal some candidate genes involved with eggshell strength at the early stage of active calcification which may facilitate our understanding of regulating mechanisms of eggshell quality.

Case Study concerning the Application of the U.S. Antidumping Law (미국반덤핑법의 적용에 관한 사례연구)

  • Ha, Choong-Lyong;Han, Na-Hee
    • International Commerce and Information Review
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    • v.10 no.3
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    • pp.143-162
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    • 2008
  • The Title 19 of the U.S. Code covers custom duties and is the heart of international trade regulation in the U.S.. Among the provisions in Title 19, is Chapter 4, the Tariff Act of 1930. Under U.S. Antidumping duty law, dumping occurs when `subject merchandise' is imported into the U.S. and sold at less than `fair value.' The administration of U.S. Antidumping duty law is shared between the Department of Commerce('Commerce') and International Trade Commission('USITC'). The U.S. Court of International Trade ("CIT") and the U.S. Court of Appeals for the Federal Circuit ("CAFC") decided the review of antidumping duty ("AD") determinations and administrative review results issued by the Commerce and the USITC, as well as the review of countervailing duty ("CVD") decisions. In Eurodif S.A. v. United States, the CAFC considered the important issue of whether the antidumping and countervailing duty laws apply to sales and purchases of services--in this case, the sale or purchase of enrichment services. Although the federal courts had considered the issue of whether a sale of enrichment services constitutes a sale of goods, the issue had never arisen in the context of the antidumping and countervailing duty laws. Also this is the first time that the Supreme Court has ever agreed to consider an antidumping case.

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A Study on the Pollutant Reduction by Venturi Type After-burner (벤츄리형 후연소기의 오염물질 저감에 관한 연구)

  • Lee, Hwa-Sin;Lee, Yong-Hoo;Lee, Jin-Seok;Kwon, Oh-Boong;Lee, Do-Hyung
    • Proceedings of the Korean Society of Marine Engineers Conference
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    • 2005.06a
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    • pp.81-86
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    • 2005
  • The purpose of this study is to develop venturi type after-burner in order to obtain pollutant reduction effect and find the best stable combustion condition. For this purpose, through a flow analysis, the shape of venturi type was made and flame holder locations were also decided by measuring chemical species at before and after the after-burner. Also, various chemical species concentration were measured at changing the induced air rates and the oxygen for oxygen enrichment for the solution the problems of much oxygen flow rate and the flame stability range. As results of this study, a flow distribution and the purification effect was excellent at venturi contraction 0.5 and flame holder location 12mm below the center of Venturi throat. On the purification characteristics, we found that pollutants reduction was effective when area ratio and oxygen are increased. But there are suitable quantities due to the flame shape change and combustion efficiency.

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The Effect of Preferable Enrichments in the Laboratory Minipigs

  • Jeon, Ryoung-Hoon;Kim, Seung-Joon;Lee, Won-Jae
    • Journal of Embryo Transfer
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    • v.32 no.4
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    • pp.305-310
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    • 2017
  • Miniature pig (minipig) has been considered as an important laboratory animal in the developmental biotechnology researches with respect to xenotransplantation, stem cell, somatic cell nuclear transfer and embryo transfer. Given that the laboratory minipigs are normally housed at an indoor facility, they pass the time with lying or sleeping unless it is feeding time. Therefore, it is necessary to provide environmental enrichments to satisfy their innate needs and to lessen atypical behaviors caused by stress, on the purpose of welfare. We quantitatively investigated the type of preferable enrichment for the laboratory minipigs as well as its effect on their daily life. They presented a great interest to the pliable pail but a rapid loss of attraction to non-preferable enrichments. When the daily life of the single housed minipigs was quantified based on duration of playing or resting, they were more actively engaged in lively activities in the presence of enrichments. In addition, the provision of enrichments could effectively alleviate the conflicts during group housing when new pen mate was introduced, resulting in reduction of wound cases. We believe the considerations of animal welfare are essential to the conduct of better research because animals in the non-stressful environment will be more physiologically stable and provide more reliable results in the animal experiments.

Enrichment of Peptides using Novel C8-functionalized Magnetic Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Analysis

  • Song, Sun-Mi;Yang, Hyo-Jik;Kim, Jin-Hee;Shin, Seong-Jae;Park, Eun-Hye;Kim, Jeong-Kwon
    • Mass Spectrometry Letters
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    • v.2 no.2
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    • pp.53-56
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    • 2011
  • [ $C_8$ ]functionalized magnetic nanoparticles were synthesized by coating magnetic $Fe_3O_4$ nanoparticles with silicaamine groups using 3-aminopropyltriethoxysilane and by subsequently modifying the amine groups with chloro(dimethyl)octylsilane to produce octyl groups on the surface of the MNPs. The $C_8$-functionalized MNPs were used to enrich peptides from tryptic protein digests of myoglobin and ${\alpha}$-casein. The enriched peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-MS was also used to investigate desalting of the $C_8$-functionalized MNPs. Sample solutions were prepared in 1.0 M NaCl, and the successful removal of salt was observed. Enrichment with $C_8$-functionalized MNPs was very effective for separating and concentrating tryptic peptides.

Characteristics of Heavy Metallic Elements of PM10 for Yellow sand and Non-Yellow sand during Springtime of 2002 at Busan (2002년 부산지역 봄철 황사/비황사시 PM10 중의 중금속 농도 특성)

  • Jeon, Byung-Il
    • Journal of Environmental Impact Assessment
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    • v.12 no.2
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    • pp.99-108
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    • 2003
  • We collected and analyzed PM10 samples to account for the characteristics of heavy metallic elements for yellow sand and non-yellow sand during springtime of 2002 at Busan, The mean PM10 mass concentration for springtime of 2002 was $219.82{\mu}g/m^3$ with the maximum $787.50{\mu}g/m^3$ and the minimum $19.44{\mu}g/m^3$. The mean concentration of metallic elements contained in PM10 are shown as follows : Si>Ca>Fe>Al>Na, respectively. The ratio of mean PM10 mass concentration for yellow sand($362.7{\mu}g/m^3$) to that for non-yellow sand($48.3{\mu}g/m^3$) was 7.5, the significant positive correlation (P<0.05) was found between yellow sand and non-yellow sand. The metallic elements concentration ratios of yellow sand to the non-yellow sand were over 10 times for Al, Ca, Mg, 4~8 times for Fe, Si, Mn. But the concentration of Na, Cu, Zn for non-yellow sand was higher than those of yellow sand. The crustal enrichment factor of Cd, Cu, Pb, Zn, Cr, K, Mn, Na, Ni for yellow sand was higher that of non-yellow sand over 10 times, and concentration rate of soil particles of yellow sand was increased 2.3 times that of nonyellow sand.

Response of Soybean Growth to Elevated $CO_{2}$ Conditions

  • Kim Young-Guk;Lee Jae-Eun;Kim Sok-Dong;Shin Jin-Chul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.303-309
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    • 2006
  • The study examined the effects of $CO_2$ enrichment on growth of soybean (Glycine max). Two soybean varieties were used, Taekwang and Cheongja. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the flowering stage. The plants were exposed to the two elevated $CO_2$ levels of 500 and 700 ppm and the ambient level of 350 ppm. Results of the experiment showed that at the second-node trifoliate stage of the two varieties, the elevated $CO_2$ increased plant height, leaf area and dry weight. The elevated $CO_2$ also raised the photosynthetic rate of soybean as compared to the ambient level. From the beginning bloom stage to the full maturity stage of the two varieties, the elevated $CO_2$ increased plant height, leaf area, seed weight and photosynthetic rate. The stomatal conductance and transpiration rate decreased on long days relative to short days of treatment. Through the entire stages, the elevated $CO_2$ increased the water use efficiency of soybean plants because stomatal conductance and transpiration rate decreased at the elevated $CO_2$ levels relative to the ambient level.

A Mentorship Model based on KIGES' Mentorship program (KIGES의 사사교육 프로그램에 기초한 사사교육 모형 개발)

  • Sunk, Hyun-Sook;Lee, Sang-Chun;Heo, Jung-Yun;Woo, Jeong-Soo
    • Journal of Gifted/Talented Education
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    • v.14 no.4
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    • pp.93-112
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    • 2004
  • Mentorship as an enrichment program as well as a promising educational method in the 21st century for the gifted students in science provides young scientists with opportunities to develop their abilities and attitudes regarding research. Kyungnam Institute of Gifted Education in Science (KIGES) has run mentorship program for five years. Many advantages of mentorship program for the science gifted students are reviewed including those of KIGES. A mentorship model for future student research programs is proposed as a way to facilitate the process of mentorship.

Factors Associated with Use and Types of Multiple Concurrent Care and Education Arrangements in Early Childhood in South Korea

  • An, Miyoung
    • International Journal of Human Ecology
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    • v.15 no.2
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    • pp.81-92
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    • 2014
  • This paper explores factors associated with multiple concurrent care and education arrangements in early childhood in South Korea. It draws on a subsample from the Korean Longitudinal Survey of Women and Families. Results show that about one-fifth of the families utilized multiple arrangements for their first preschool child. The primary non-parental option in multiplicity was nurseries or kindergartens. Home care and education options such as home study materials were found to be most prevalent secondary non-parental option, followed by services at private institution as well as relative care. Children's age, care cost, non-parental care time and time constraints were found to be positively related to the incidence of multiplicity for the first preschool child while family income and cost constraints were negatively associated. As a secondary option in the multiplicity, services at private institutions increased with children's age, care cost and when the grandparents live far from the parents' house. Home care and education utilization was found more among mothers with low degree of time constraints. Utilization of relative support decreased with the children's age and meant lower care cost and increased with mother's employment, fathers' education, family income, cost constraints and when grandparents live nearby. This paper, based on the associated factors, suggests how issues of children enrichment and constraints might be related to the multiplicity.

Enrichment Measurement of Nuclear Materials by Passive Gamma-ray Analysis (수동적 감마선분석에 의한 핵물질 농축도 측정)

  • Hong, Jong-Sook;Cha, Hong-Ryul;Park, Hyoung-Nae;Lee, Byung-Doo;Park, Ho-Joon
    • Nuclear Engineering and Technology
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    • v.23 no.2
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    • pp.233-240
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    • 1991
  • U-235 enrichment has been measured non-destructively by passive gamma-ray pulse height analysis. Measurement source is 185.7 keV gamma-ray which is emitted from uranium sample during alpha decay of U-235 in it. Factors influencing the measurement such as sample composition, attenuation effect of container wall, collimation effect and counting efficiency were evaluated. Under the optimized counting system, the measured relative errors were~8%, ~8% and~1% from Tag values at 95% confidence level for depleted UF$_{6}$ cylinders, depleted UO$_2$powder, and natural UO$_2$powder respectively.

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