• KSBB Journal
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• v.17 no.6
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• pp.549-554
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• 2002

The hydrolysis of old book(Hanji) was performed using endoglucanase Ⅰ(endo Ⅰ), and exoglucanase II(exe II) and their mixtures purified from Trichoderma viride cellulase. The optimum degradation of old book(Hanji) with endo Ⅰ, exo II and endo-exo mixture(Ⅰ：Ⅰ) were exhibited at pH 4.5, 5.5, 5.0, respectively. Maximum degradations using endo Ⅰ, exo II and endo-exo mixture(Ⅰ：Ⅰ) occurred at 50$\^{C}$. The yield decreased an increasing the enzyme concentration. Especially, the yield was lowest for treatment with the endo Ⅰ-exo II mixture(Ⅰ：Ⅰ), which may be regarded as being due to a synergistic action of the cellulase components. Physical strength increased with increasing exo II concentration, and decreased with increasing concentration of endoglucanase Ⅰ. These results indicated that the degradation of old book(Hanji) depends largely upon the action of endoglucanase. Therefore, the most effective method of conserving paper cultural properties is to repress the action of endoglucanase.

• Microbiology and Biotechnology Letters
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• v.33 no.4
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• pp.267-273
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• 2005

To develop effective and powerful probiotic, Saccharomyces cerevisiae strains producing cellulolytic enzymes were genetically brooded. For the production of exoglucanase, the plasmid pVT-TExo (8.8 kb) was constructed, in which Thermomonosporafusca exoglucanase gene (E3) was under the control of ADHl promoter, and introduced into S. cerevisiae SEY2102. When the transformant, S. cerevisiae SEY2102/pVT-TExo, was cultivated on YPD medium, the total expression level of avicelase reached about 190 unit/l. The secretion efficiency and plasmid stability were about $50\%\;and\;91\%$, respectively. Recombination exoglucanase enzyme bound to avicel better than Clostridium endoglucanase (CelA) and Trichoderma endoglucanase (C4) enzymes. The mixing ratio of E3 and CelA displaying the best synergistic hydrolysis for avicel was observed at 4:1. The mixture of endoglucanase (CelA) and exoglucanase (E3) resulted in 3.2-fold increase of avicelase activity and 2.5-fold enhanced production of sugar production from avicel, compared to the single enzyme treatment.