• Journal of Digital Convergence
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• v.14 no.11
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• pp.91-100
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• 2016

The purpose of this study was to draw out success factors of port operation for New International Passenger Terminal(NIPT) in Incheon Port using the fuzzy method. In this study, the success factors of port operation were firstly selected by literature review and finally adopted them by in-depth interview with experts who had working experiences over 15 years in the field of Korea-China Carferry shipping business and stevedoring business. Success factors of port operation of the NIPT have been classified into four principal factors such as 'land's use side', 'productivity side', 'cost side' and 'services side', and the comparative analysis was conducted among success factors of port operation. According to the results of analysis, in terms of "land's use side", securing CY space of full-empty containers(0.857) was the most important factor. Among detailed factors of "productivity side", building terminal automation system(0.806) was significantly needed. In the factors of "cost side" and "service side", land rental costs(0.861) and schedule management of berth operation(0.798) were selected as the most important factors. Consequently, new international passenger terminal has implications that the main port operations should focus on reasonable rents calculation, ensuring proper space of CY, and building automation system of terminal, There is a need to proceed to conduct a comparative study of the same operating mode of an international passenger terminal.

• Journal of Life Science
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• v.26 no.10
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• pp.1182-1188
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• 2016

Membrane-associated guanylate kinase inverted-3 (MAGI-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs). MAGI-3 modulates the kinase activity of protein kinase B (PKB)/AKT through interactions with phosphatase and tensin homolog (PTEN)/MMAC. Coxsackievirus B3 (CVB3) is a common causative agent of acute myocarditis and chronic dilated cardiomyopathy. Activation of AKT and extracellular signal-regulated kinases 1/2 (ERK1/2) is essential for CVB3 replication, but the relation between MAGI-3 signaling and CVB3 replication is not well understood. This study investigated the role of MAGI-3 in CVB3 infection and replication. MAGI-3 was overexpressed in HeLa cells by polyethylenimine (PEI) transfection. To optimize the transfection conditions, different ratios of plasmid DNA to PEI concentrations were used. MAGI-3 and empty plasmid DNA were transfected into the HeLa cells. MAGI-3 overexpression alone was not sufficient to efficiently activate AKT. However, expression of the CVB3 capsid protein VP1 dramatically increased in the HeLa cells overexpressing MAGI-3 24 h after CVB3 infection. In addition, the activities of AKT and ERK were significantly induced in the CVB3-infected MAGI-3 cells overexpressing HeLa. These results demonstrate that MAGI-3 expression upregulates CVB3 replication through AKT and ERK signaling activation. MAGI-3 may be an important target to control CVB3 replication.