• Title/Summary/Keyword: elongation of E

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Identification of Proteins Phosphorylated by Protein Kinase C in Soybean (대두 유식물에서 Protein Kinase C에 의해서 인산화되는 단백질의 동정)

  • 최윤희
    • Journal of Plant Biology
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    • v.35 no.1
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    • pp.85-90
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    • 1992
  • The previous report (Chung and Lee, 1992) in our laboratory demonstrated that the protein kinase C (PKC) activator, TPA, promotes the elongation of corn coleoptiles significantly. To understand the role of TPA on the growth, substrates of PKC were investigated using PKC partially purified from soybean by DEAE-52 cellulose column. The enzyme activity increased about 5-fold in the presence of $Ca^{2+}$, phosphatidylserine and diolein compared with that in the absence of these reagents. Phosphorylation of both cytosol and membrane proteins by the purified PKC increased in the presence of $Ca^{2+}$ compared with that of EGTA treatment. However, the phosphorylation did not increase markedly by treatment with TPA or phosphatidylserine and diolein in the presence of $Ca^{2+}$ compared with $Ca^{2+}$ alone. The decrease, in phosphorylation of 100, 61 and 43 Kd proteins of the cytosol, and 140, no, 66, 47 and 32 Kd membrane proteins in hypocotyls, and 140, no, 66, 47, 33, 31 and 16 Kd membrane proteins in the root was observed in the presence of PKC inhibitor staurosporine (5T A). These results suggest that subatrates of PKC in soybean may be 110, 63 and 41 Kd proteins of the cytosol, and 140, 110, 66, 47 and 32 Kd membrane proteins in the subapical region of the hypocotyl, and 140, 110, 66, 47, 33, 31 and 16 Kd membrane proteins of the root.e root.

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Influence of Blend Mode of Extender Oil on the Properties of EPDM/PP-Based Thermoplastic Vulcanizates (이피디엠/폴리프로필렌 열가소성 경화물에서 오일의 블렌드 방식이 경화물의 물성에 미치는 영향)

  • Na, Sung-Su;Song, Ki-Chan;Kim, Su-Kyung
    • Elastomers and Composites
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    • v.44 no.3
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    • pp.315-322
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    • 2009
  • Influence of blend mode of extender oil on the properties of thermoplastic vulcanizates (TPVs), based on an ethylene-propylene-diene copolymer (EPDM) and a polypropylene (PP), was studied. The EPDM/PP TPVs were prepared in an open roll mill using two different modes in blending sequence of paraffinic oil and phenolic curative, i.e., Oil-Cure and Cure-Oil modes. Degree of cross-linking by gel fraction and properties such as hardness, tensile strength, elongation at break, and melt flow rate were investigated as a function of extender oil content for the two modes. Little influence of the blend mode of extender oil on the degree of cross-linking and mechanical behaviors was observed. However, the use of Cure-Oil mode in the preparation of EPDM/PP TPVs resulted in a marked increase in the level of processability as reflected by melt flow index, as compared to the use of Oil-Cure mode.

Mechanical and Biological Characteristics of Reinforced 3D Printing Filament Composites with Agricultural By-product

  • Kim, Hye-Been;Seo, Yu-Ri;Chang, Kyeong-Je;Park, Sang-Bae;Seonwoo, Hoon;Kim, Jin-Woo;Kim, Jangho;Lim, Ki-Taek
    • Food Engineering Progress
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    • v.21 no.3
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    • pp.233-241
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    • 2017
  • Scaffolds of cell substrates are biophysical platforms for cell attachment, proliferation, and differentiation. They ultimately play a leading-edge role in the regeneration of tissues. Recent studies have shown the potential of bioactive scaffolds (i.e., osteo-inductive) through 3D printing. In this study, rice bran-derived biocomposite was fabricated for fused deposition modeling (FDM)-based 3D printing as a potential bone-graft analogue. Rice bran by-product was blended with poly caprolactone (PCL), a synthetic commercial biodegradable polymer. An extruder with extrusion process molding was adopted to manufacture the newly blended "green material." Processing conditions affected the performance of these blends. Bio-filament composite was characterized using field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Mechanical characterization of bio-filament composite was carried out to determine stress-strain and compressive strength. Biological behaviors of bio-filament composites were also investigated by assessing cell cytotoxicity and water contact angle. EDX results of bio-filament composites indicated the presence of organic compounds. These bio-filament composites were found to have higher tensile strength than conventional PCL filament. They exhibited positive response in cytotoxicity. Biological analysis revealed better compatibility of r-PCL with rice bran. Such rice bran blended bio-filament composite was found to have higher elongation and strength compared to control PCL.

Genetic Diversity and Population Structure Analyses of SSIV-2 Gene in Rice

  • Thant Zin Maung;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.212-212
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    • 2022
  • Soluble starch synthase (SS) IV-2 is one of the starch synthase gene family members and responsible for starch chain elongation interacting with other rice eating and cooking quality controlling genes (e.g., AGPlar and PUL). SSIV-2 is mainly expressed in leaves, especially at grain-filling stage and its alleles can significantly affect rice quality. Here, we investigated the genetic diversity and population structure analyses of SSIV-2 gene by using 374 rice accessions. This rice set was grouped into 320 cultivated bred (subsequently classified into temperate japonica, indica, tropical japonica, aus, aromatic and admixture) and 54 wild rice. Haplotyping of cultivated rice accessions provided a total of 7 haplotypes, and only three haplotypes are functional indicating four substituted SNPs in two exons of chromosome 5: T/A and G/T in exon 4, and C/G and G/A in exon 13. Including the wild, a highest diverse group (0.0041), nucleotide diversity analysis showed temperate japonica (0.0001) had a lowest diversity value indicating the origin information of this gene evolution. Higher and positive Tajima5s D value of indica (1.9755) indicate a selective signature under balancing selection while temperate japonica (-0.9018) was in lowest Tajima's D value due to a recent selective sweep by positive selection. We found the most diverse genetic components of the wild in PCA but shared in some portion with other cultivated groups. Fixation index (FST-values) and phylogenetic analysis indicate a closer relationship of the wild with indica (FST=0.256) than to its association to both of temperate japonica (FST=0.589). Structure analysis shows a clear separation of cultivated subpopulations at every K value, but genetic components were admixed within the wild illustrating the same genetic background with japonica and indica in some proportion.

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Promotion of Plant Growth by Submergence and the Action Network of Hormones (침수에 의한 식물의 생장 촉진과 호르몬들의 작용 네트워크)

  • Cho Young Jun;Lee Young Na;Park Woong June
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.112-117
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    • 2005
  • Plants living riverside show diverse resistance responses to submergence. The promoted petiole elongation of semi-aquaitc plants, e.g., such as Ranunculus sceleratus and Rumex palustris, is one of the adaptive responses mediated by the plant hormone ethylene. The gaseous hormone is trapped in submerged plant tissues and enhances the petiole growth by increasing sensitivity of the tissues to some plant hormones including auxin. Due to the stimulated growth of petioles, the leaves finally reach the water surface and can respirate again. At the water surface, the accumulated ethylene diffuses out from the tissues to the air. As a result, the increased hormone sensitivity decreases again, and thus the growth rate reduces to the basal level as before. The increased auxin sensitivities by ethylene observed in Ranunculus sceleratus, revealed by the changes in the auxin dose-response curves, indicate the increase of affinities of the receptors to auxin. However, the molecular mechanism of the affinity regulation remains still largely unknown, because the identity of the auxin receptor is still unclear.

Characterization of the Material Properties of Sheet Metal for Auto-body at the High Strain Rate Considering the Pre-strain Effect (예비변형률 효과를 고려한 고변형률 속도에서의 차체용 강판의 물성 특성)

  • Kim, Seok-Bong;Lim, Ji-Ho;Huh, Hoon;Lim, Jong-Dae
    • Transactions of the Korean Society of Automotive Engineers
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    • v.12 no.3
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    • pp.204-210
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    • 2004
  • Most auto-body members fabricated by the sheet metal forming process. During this process the thickness and material properties of the sheet metal are changed with the residual stress and plastic strain. This paper deals with the material properties of the sheet metal at the high strain rate considering the pre-strain effect. Specimens are selected from sheet metals for outer panels and inner members, such as SPCEN, SPRC45E, SPRC35R and EZNCD. The specimens are prepared with the pre-strain of 2, 5 and 10 % by tensile elongation in Instron 5583, which could be equivalent to the plastic strain in sheet metal forming. High speed tensile tests are then carried out with the pre-stained specimens at the strain rate of 1 to 100/sec. The experimental result informs that the material properties are noticeably influenced by the pre-strain when the yield stress of the specimens is moderate as SPCEN, SPRC35R and EZNCD. The result also demonstrates that the ultimate tensile strength as well as the yield stress is increased as the amount of the pre-strain is increased.

Development of Rubber Composite Materials Using Waste EPDM (폐 EPDM을 이용한 고무 복합 소재 개발)

  • Park, Dong-Kyu;Hong, Yeo-Joo;Jeong, Keuk-Min;Kim, Jin-Kuk
    • Elastomers and Composites
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    • v.47 no.2
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    • pp.121-128
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    • 2012
  • Waste EPDM(W-EPDM) collected from the automotive weather strip and the gasket of a laundry machine has not been effectively recycled. Using this W-EPDM powder and other ingredients, i.e., binder(polyolefin resin, polyolefin elastomer, etc.), filler and additives, various economic rubber composites were made by extrusion. In advance of main experiments, the effects of ultrasonic treatment of W-EPDM on the property of rubber composites, comparison in the property of the composites of W-EPDM with those of virgin and devulcanized EPDM, and waste tire rubber were investigated. Also, the properties of the rubber composites extruded with a 12-screw extruder were compared with those extruded with twin-screw extruder. Various W-EPDM composites for synthetic turf filler and car mat were extruded and injection molded, and 3 main properties of tensile strength, elongation and hardness were investigated to develop economical and proper recipes of the rubber composites.

Effects of Light-Quality Control on the Plant Growth in a Plant Factory System of Artificial Light Type (인공광 식물공장내 광질 제어가 작물생육에 미치는 영향)

  • Heo, Jeong-Wook;Baek, Jeong-Hyun
    • Korean Journal of Environmental Agriculture
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    • v.40 no.4
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    • pp.270-278
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    • 2021
  • BACKGROUND: Horticultural plant growth under field and/or greenhouse conditions is affected by the climate changes (e.g., temperature, humidity, and rainfall). Therefore investigation of hydroponics on field horticultural crops is necessary for year-round production of the plants regardless of external environment changes under plant factory system with artificial light sources. METHODS AND RESULTS: Common sage (Salvia plebeia), nasturtium (Tropaeolum majus), and hooker chive (Allium hookeri) plants were hydroponically culturing in the plant factory with blue-red-white LEDs (Light-Emitting Diodes) and fluorescent lights (FLs). Leaf numbers of common sage under mixture LED and FL treatments were 134% and 98% greater, respectively than those in the greenhouse condition. In hooker chives, unfolded leaf numbers were 35% greater under the artificial lights and leaf elongation was inhibited by the conventional sunlight compared to the artificial light treatments. Absorption pattern of NO3-N composition in hydroponic solution was not affected by the different light qualities. CONCLUSION(S): Plant factory system with different light qualities could be applied for fresh-leaf production of common sage, nasturtium, and hooker chive plants culturing under field and/or greenhouse. Controlled light qualities in the system resulted in significantly higher hydroponic growth of the plants comparing to conventional greenhouse condition in present.

A Study on the Mechanical and Hand Properties of Knitted Fabrics - Focused on the 2 Colors Jacquard - (편성물의 역학적 특성과 태 평가 - 2 칼라 자카드를 중심으로-)

  • Koo, Mi-Ran
    • Journal of the Korea Fashion and Costume Design Association
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    • v.12 no.3
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    • pp.93-103
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    • 2010
  • The results of comparison and analysis of dynamical features according to Jacquard structure are as follows. Regarding elongation(E) value in tensile property, the value in the direction of course was found to be larger in all Jacquard structure, except floating Jacquard, than the direction of wale. It could be found that, as to bending strength(B) in Bending Property, the value of bending strength in the direction of course smaller in all the textile(structure) except floating than the direction of wale, so that the ability of curve formation is excellent. And in case of floating Jacquard, it showed the smallest value in the direction of course and wale, so that it was interpreted as Jacquard having a soft feel. Blister Jacquard showed the highest value in both directions of course and wale, so that it was evaluated as Jacquard having the hardest touch. With regard to shearing character(G) and shearing hysteresis(2HG) in Shearing Property, Jacquard indicated the lowest value, so it was evaluated as the textile(structure) having high drape the transformation of whose fabric is easy. In addition, normal Jacquard, transfer Jacquard, blister Jacquard showed a high value, so that it could be known they are Jacquards having stiff touch. The study implies that normal Jacquard, bird's eye Jacquard, etc., which well stretch in the direction of a course like tubular Jacquard course, is better to make the margin a little shorter than the basic margin, and Jacquard that well stretches in the direction of wale like ladder's back Jacquard and floating Jacquard is better to make the margin longer and the length shorter than the basic margin.

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Computational Analysis of Tumor Angiogenesis Patterns Using a Growing Brain Tumor Model

  • Shim, Eun-Bo;Kwon, Young-Keun;Ko, Hyung-Jong
    • International Journal of Vascular Biomedical Engineering
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    • v.2 no.1
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    • pp.17-24
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    • 2004
  • Tumor angiogenesis was simulated using a two-dimensional computational model. The equation that governed angiogenesis comprised a tumor angiogenesis factor (TAF) conservation equation in time and space, which was solved numerically using the Galerkin finite element method. The time derivative in the equation was approximated by a forward Euler scheme. A stochastic process model was used to simulate vessel formation and vessel elongation towards a paracrine site, i.e., tumor-secreted basic fibroblast growth factor (bFGF). In this study, we assumed a two-dimensional model that represented a thin (1.0 mm) slice of the tumor. The growth of the tumor over time was modeled according to the dynamic value of bFGF secreted within the tumor. The data used for the model were based on a previously reported model of a brain tumor in which four distinct stages (namely multicellular spherical, first detectable lesion, diagnosis, and death of the virtual patient) were modeled. In our study, computation was not continued beyond the 'diagnosis' time point to avoid the computational complexity of analyzing numerous vascular branches. The numerical solutions revealed that no bFGF remained within the region in which vessels developed, owing to the uptake of bFGF by endothelial cells. Consequently, a sharp, declining gradient of bFGF existed near the surface of the tumor. The vascular architecture developed numerous branches close to the tumor surface (the brush-border effect). Asymmetrical tumor growth was associated with a greater degree of branching at the tumor surface.

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