• Journal of the Korean Society of Costume
• /
• v.60 no.2
• /
• pp.35-50
• /
• 2010

In this dissertation, the analysis of the study of female customs from late Joseon Dynasty and their genre painting to research humors and its aesthetic senses in that certain era. The purpose of the genre paintings is to look at general populations in an objective point of view and endeavor better value of public life style to embody clear understandings of humanity. The artists such as Hong-Do Kim and Youn-Bok Shin expressed their arts based on reasonable reality with sarcastic but humorous and creative ways to criticize the society's problems with clear statements. Therefore, the formative characters are realism, exaggeration and coloring of the whole arts that represents as innovative and original genre of it's time. Hong-Do Kim and Youn-Bok Shin expressed the women in society as open minded with versatile and refined looks in their paintings as the reality was repressed and closed mind for women figures in late Joseon Dynasty. The female customs in the paintings has both suppression and expression in their dresses and exaggerated shapes of accessories and the use of color were also found. All of these elements has the aesthetic values of satirized eroticism, the hint of next evolution of the late Joseon Era under the conservative disposition of social characters and freedom of expression of playfulness. These elements were new developments and a step forward of female 'sex' and expression of satirized eroticism. The exaggerated and distorted forms and accessories demonstrates unbalanced and asymmetrical elements in humorous characters and they include spontaneity. Also, using the five traditional color schemes of Korean art displays humor in playfulness of an art with splendid and purity, duality of positiveness and artless, smart and elegance looks.

• Journal of Digital Convergence
• /
• v.15 no.9
• /
• pp.427-434
• /
• 2017

The purpose of this study is to analyze the BI elements of expression for four brand's experience spaces in Korea and propose a direction for improvements for such spaces. I started with the theoretical background of nowadays brand marketing and experiential BI elements through literature researches, and then researched the property of spatial expressions to analyze BI elements. Based on this research, I analyzed four domestic brand experience spaces. As a result, brand experience spaces must clearly express the brand image of the company through various contents and situations to effectively communicate and improve BI expressions. Also, they need long-term experience contents for sustained relationships rather than short-term experiences. I expect this study will be a good resource for BI expression research in brand experience spaces. I also believe that this study can guide other studies about BI expression in other fields.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.17 no.9
• /
• pp.2286-2293
• /
• 1993

The study presents the development of a cost-effective CAD/CAM system for metal moulds by use of personal computer. In the personal CAD/CAM system named DKSYS, metal moulds are defined by the operations of basic elements such as prism, pyramid and sphere, plane, rotational body, and surface elements including curved surface. The internal expression of shape elements in the computer is based on the wire model, which is a set of cross-sectional curves of the shape elements. With the addition of OMS system to CAD/CAM system the optimum cutting condition can be selected automatically. After programming NC information with the form definition and the optimum cutting condition, the metallic mould can be formed by transferring cutting information to CNC machine through DNC system. Using a computer for the above process, it is possible to increase the productivity and reduce the cost.

• Journal of Korean Classical Literature and Education
• /
• no.15
• /
• pp.139-161
• /
• 2008

This study examined the texts of the Late Joseon Dynasty's YEONJEONG GASA, whose authors are unknown, to list the contents of education which can be provided by conventional elements. Most existing discussions on expression education have focused on creativity. However, the perspective creativity based on the concepts of modern individualism and difference believes that creation can be done only by special professionals with inborn abilities and is likely to jeopardize the possibility and need of expression education to reduce the scope of expression education. On the other hand, conventionality has been related to "cliche" and "old-fashioned" and considered the counterpoint of creativity with lacking originality and aesthetical or educational valuelessness. Nevertheless, conventionality is valuable as cultural custom in that it is closely related to the contemporary culture, thoughts, and language. Also, it is the power that creates new text and induces sympathy in receivers. The Late Joseon Dynasty's YEONJEONG GASA by unknown authors include typicality of circumstances, schematism of emotion, and formula in conventionality. The positive aspects of conventionality - familiarity and intimacy - are the basis of sympathy and become the power that encourages the public to participate in creation and enjoyment within the frame of creativity. Ultimately, conventionality of YEONJEONG GASA allows learners to experience the pleasure of expression and has an important meaning for expression education as it provides them with the opportunity to perform expressions to extend sympathy.

• Proceeding of Spring/Autumn Annual Conference of KHA
• /
• 2009.11a
• /
• pp.223-228
• /
• 2009

The meaning of the characteristics of formative expression in contemporary residential architecture is flowing elements as important means to inbreathe emotional feeling of movement and as automatic and positive wats of expression. So the purpose of this study was to analyze the characteristics of formative expression in contemporary residential architecture, and to plan residential design linked with this kind of concept. For this study, analysis of case studies were used and the cases were selected in contemporary residential architecture which were represented having formative expressions since 2005. The major results found that the characteristics of formative expression were ex-center, reiteration, dispersibility, diagonality, plasticity, non-linearity, and deconstruction. In addition to that, we derived various methods expressing the formative characteristics, which were mass transformation, plan variation and composition element transformation. Summing these results up, the various characteristics of formative expression in contemporary residential architecture are understood as the results of the free modeling will of architects fulfilled with the spirit of inquiry and experiment to escape from the standard architecture in order to differentiate themselves.

• Journal of fish pathology
• /
• v.24 no.3
• /
• pp.297-305
• /
• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Toxicological Research
• /
• v.31 no.1
• /
• pp.1-9
• /
• 2015

Environmental toxicants such as toxic metals can alter epigenetic regulatory features such as DNA methylation, histone modification, and non-coding RNA expression. Heavy metals influence gene expression by epigenetic mechanisms and by directly binding to various metal response elements in the target gene promoters. Given the role of epigenetic alterations in regulating genes, there is potential for the integration of toxic metal-induced epigenetic alterations as informative factors in the risk assessment process. Here, we focus on recent advances in understanding epigenetic changes, gene expression, and biological effects induced by toxic metals.

• Journal of Microbiology
• /
• v.33 no.3
• /
• pp.196-200
• /
• 1995

In order to express the CDC70 gene of Saccharomyces cerevisiae by heat shock, we have designed heat inducibe hybrid promoters using the Drosophila melanogaster heat shock elements (HSEs). A 220 bp-long upstream fragment of the D. melanogaster hsp70 gene comprised of four HSEs was placed upstream of the putative proximal TATA box of the CDC70 gene. Hybrid promoters containing different fusion joints were tested for their ability to drive the CDC70 gene expression by heat shock. The results showed that the HSEs of D. melanogaster conferred the heat-induced CDC70 gene expression, but the heat inducibility was much lower than that in D. melanogaster.

• KSBB Journal
• /
• v.16 no.1
• /
• pp.1-10
• /
• 2001

Escherichia coli has been used as an expression work horse for foreign genes. This article summarized recent development in genetic engineering techniques for overproduction of medical proteins and industrial enzymes. Special emphasis was placed upon research activities concerning folding and refolding of inclusion bodies at genetic and fermentation levels. Plasmid and mRNA stabilization, development of strong inducible promoters, modification of translational elements and reduction of rpoteolytic degradation were carried out to elevate an expression level of a target protein. Optimization of culture conditions, improvement of denaturation and renaturation steps and coexpression of molecular chaperones or foldase were accomplished to produce active proteins in soluble form. Fusion protein systems with selective separation and surface display technology were also performed in an effort to make the E. coli expression system more effective and versatile.

• Molecules and Cells
• /
• v.45 no.8
• /
• pp.522-530
• /
• 2022

Transposable elements (TEs) account for approximately 45% of the human genome. TEs have proliferated randomly and integrated into functional genes during hominoid radiation. They appear as right-handed B-DNA double helices and slightly elongated left-handed Z-DNAs. Human endogenous retrovirus (HERV) families are widely distributed in human chromosomes at a ratio of 8%. They contain a 5'-long terminal repeat (LTR)-gag-pol-env-3'-LTR structure. LTRs contain the U3 enhancer and promoter region, transcribed R region, and U5 region. LTRs can influence host gene expression by acting as regulatory elements. In this review, we describe the alternative promoters derived from LTR elements that overlap Z-DNA by comparing Z-hunt and DeepZ data for human functional genes. We also present evidence showing the regulatory activity of LTR elements containing Z-DNA in GSDML. Taken together, the regulatory activity of LTR elements with Z-DNA allows us to understand gene function in relation to various human diseases.