• Applied Biological Chemistry
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• v.37 no.5
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• pp.361-369
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• 1994

To study the light-induced expression mechanism and protein transport into the chloroplast, a rice genomic clone (GrbcS) for the small subunit of ribulose 1,5-bisphosphate carboxylase (rbcS) was isolated and its nucleotide sequence was determined. Nucleotide sequence analysis of GrbcS revealed that the gene consists of two exons interrupted by an intron, encoding a protein of 175 amino acids including a transit peptide of 47 amino acids. These structural features of GrbcS are consistent with those of other rbcS genes from monocot species. Genomic Southern blot analysis suggested that the rbcS genes are present as a relatively small multigene family in the rice genome. Comparison of the nucleotide and deduced amino acid sequences to other rice rbcSs shows close sequence similaritiy. Conserved DNA sequences present in other light-responsive genes are also found in the 5’ upstream region of GrbcS such as G-box, 3AF1-binding site and GATA site. The possible function of these putative regulatory elements are discussed.

• The Journal of the Korea Contents Association
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• v.21 no.5
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• pp.889-904
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• 2021

The planning of traditional urban has to face great challenges under the influence of the uncertainty and mobility of contemporary cities. While for this kind of challenge, it has brought the chance to develop Landscape Urbanism quickly. As an important practice place for Landscape Urbanism, open space provides a platform for many landscape designers to display. The purpose of this research is to explore the expression of the core content of Landscape Urbanism in open space, and propose the design tendency of open space in Korea. According to the constitution elements of urban open space and the core concept of Landscape Urbanism, this thesis establishes the analysis framework, which carries out the case empirical analysis for the open space of New York. Through case analysis, we can see that there are five major characteristics of Landscape Urbanism. That is, the integration or imitation of natural terrain, green infrastructure construction, emphasizing ecological resilience, adaptability to unplanned events, and analyzing the site from multiple scales. In this research, the design proposal proposed on the basis of Landscape Urbanism is able to provide enlightenment for the urban open space design of Korea in the future.

• Journal of Korea Entertainment Industry Association
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• v.14 no.1
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• pp.117-124
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• 2020

Chinese youth films fall into the dilemma of continuous development after experiencing rapid development from 2013 to 2017. How to update the creative ideas and methods of the films, arouse the audience's interest in watching and stimulate a new aesthetic experience has become a focal point for the creators who concern about the Chinese film market. "Us and Them" is a successful youth film in 2018. Its creative practice proves that defamiliarization can provide theoretical and methodological basis for the creation of Chinese youth films. Therefore, taking the current situation of Chinese youth film as the research background, treating "Us and Them" as the research object, the defamiliarization of narrative discourse and narrative content used in the film are analyzed. First, the defamiliarization of narrative discourse composed of scenes, characters and plots makes the film "stand out" from the real world. Second, the defamiliarization of narrative content composed of metropolis adoration, self-identity and hometown affection makes the film "stand out" from the existing context. These methods of creation not only meet the needs of the contemporary, but also provide an effective reference for the creation of other youth films. Research will be needed to utilize the elements of defamiliarization through the analysis of the successful case in spite of the time change.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.23 no.3
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• pp.221-231
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• 2005

Though Digital Map Ver. 2.0 is adequate to GIS, the possibility to gain information from its external form and the ease of producing paper map had retrogressed. In this research, concerned with the problems, we plan to make Digital Map Ver. 2.0, which satisfies the conditions of GIS. It will have geometrical and logical data structure, and also possess informative ability as much as that of Ver. 1.0. For the study, the process to analyze the topographic code, color, code priority order, etc. of paper relief map, digital map Ver. 1.0, and digital map Ver. 2.0 was taken. For the topographical feature with diverse expression, we changed the portrayal of digital map Ver. 2.0 to make it fit the regulations of map portrayal. At the point of topographic code priority order, the rule is to arrange them in the same order as the real territory. However, we made a special code in the case of any change of the locational order. According to the property of this study, we observe the regulations of map portrayal, for the elements related to subjective sense, such as colors. And we give priority on the data construction when the portrayal of topographical feature and the schema of GIS database system are contradictory.

• (The) Research of the performance art and culture
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• no.20
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• pp.63-94
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• 2010

Research on Gong Ok-jin's choreography and its artistic value is still insufficient in the academia of Korea. Her life cannot be said even or smooth - she was born into an artist family with abundant innate talent and sensitivity, feeling joy and sorrow as a gypsy artist begging for food, facing and overcoming the crisis of her trademark deformed dance during the period when she played the solo performance of Changmugeuk, making a transition to the animal dance and finally to the sick dance getting her health back from the long struggle against disease. Her life, however, has always been an artistic expression and we can know that art has been the consolation and driving force of her life. In the Korean artistic tradition that integrates music, singing and dancing, it is beyond doubt that the third has been rather secondary compared with the first two. In the Changmugeuk, however, Gong Ok-jin focuses on each movement of hers, commensurate with the humorous witticism and the traditional opera of pansori. What is her message in such painstaking movements? She puts gestures ahead of words and continues them, reminding her audience of the meaning of dance and driving it into them. Especially, her deformed dance, unprecedented in choreographic history, is hard to understand if you do not sympathize with Koreans' innermost emotions. If you are to understand it, you should first feel what emotions are in Koreans' mentality to be expressed in the form of choreographic elements and what artistic values they carry. This study, in this context, is to reflect upon the original choreographic form of her Changmugeuk solo and to overview Korean dance's unique emotional values with regard to the way Korean choreographic subject matters and traditions are passed down.

• Archives of design research
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• v.18 no.2 s.60
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• pp.293-302
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• 2005

The emotion and culture of an age are reflected through several mediums. The special quality and generality are discovered through household utensils used in everyday life specially. This paper was intended as proceeding study for brand development with national empathic relationship. In this article, we were here concerned with symbolism and features for stories of traditional pattern on ancestral common household utensils, and considered about development possibility by brand of tradition pattern. As analysis for symbolism of traditional pattern, re-sorting and classifying pattern, meaning, symbol and stories of pattern based on 'Blessing notion' were set. It was found from the analysis that meaning and symbol of traditional pattern were set up and stories for them were excerpted. This study defines tradition pattern classified based on 'Blessing notion' as '8 Blessing pattern', and investigated development possibility and derivative value of it. We discovered through this study that 'Blessing patterns' function as communicator, have 4 abilities in sympathy with brand paradigm, and have 6 elements of brand identity. Furthermore, 'Blessing Patierns' have some derivative functions; cognitive intension, expression, loyalty, differentiation and symbol. This research will become basis of study for brand development having 'Empathic relationship' and foundation of 'Blessing brand' that is available to be umbrella and super brand. A further direction of this study will be to make 'Blessing brand series' and to create culture of it. We hope Korean brand market goes a step further through our studies

• The KIPS Transactions:PartB
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• v.11B no.5
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• pp.563-572
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• 2004

Sentence ion is a simplification of a sentence preserving its communicative function. It accomplishes sentence revision and concept ion simultaneously. Sentence revision is a method that resolves the discrepancy between human's thoughts and its expressed semantic in sentences. Concept ion is an expression of general ideas acquired from the common elements of concepts. Sentence ion selects the main constituents of given sentences and describes the upper concepts of them with detecting their semantic information. This enables sen fence revision and concept ion simultaneously. In this paper, a syntactic parser LGPI+ and an ontology OfN are utilized for sentence ion. Sentence abstracter SABOT makes use of LGPI+ and OfN. SABOT processes the result of parsing and selects the candidate words for sentence ion. This paper computes the sentence recall of the main sentences and the topic hit ratio of the selected sentences with the text understanding system using sentence ion. The sources are 58 paragraphs in 23 stories. As a result of it, the sentence recall is about .54 ～ 72% and the topic hit ratio is about 76 ～ 86%. This paper verified that sentence ion enables sentence revision that can select the topic sentences of a given text efficiently and concept ion that can improve the depth of text understanding.

• Cartoon and Animation Studies
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• s.25
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• pp.65-85
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• 2011

In this essay, Japanese animator Kihachiro Kawamoto's works will be analyzed. Kawamoto made Breaking of Branches is Forbidden in 1968 and was famous for puppet animation as The Demon(1972), Dojoji Temple(1976) and House of Flame(1979) used Japanese traditional elements in his works. The themes are the agony and despair of a human being, and the narrative is developed dramatically. This is possible through a variety of techniques in animation expression. For example of this are the movement of the puppets and the lighting. In the case of Kawamoto's works, above all, it can be said that the dramatic development depends on editing - the relation of each shot to the next shot. Therefore, this analysis will focus especially on the editing of The Demon, Dojoji Temple and House of Flame. Kawamoto's method of editing will be examed and the analysis will confirm that classical continuity edting by controling space and time has been used. Namely that the effect of editing enhances dramatic development of the narrative on Kawamoto's works. This study will also discuss the benefit of using cinematic methods of in animation. Eventhough it is not essential, Kawamoto chooses cinematic method editing. Through their use, he is able to absorb the audience in the traditional Japanese world which ordinarily could be too difficult to understand through puppet animation.

• Journal of Life Science
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• v.32 no.7
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• pp.491-500
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• 2022

HK shiitake mushroom mycelium (HKSMM), containing 14% β-glucan, is a health functional food ingredient individually approved by the Korea Ministry of Food and Drug Safety for liver health. The anti-inflammatory effect of a 50% aqueous ethanol extract of HKSMM (designated HKSMM50) was studied in RAW 264.7 macrophage cells treated with lipopolysaccharide (LPS). An active hexose correlated compound (AHCC) was used as a positive control. LPS-activated RAW 264.7 cells were treated with HKSMM50 and AHCC (0, 20, 100, 500 ㎍/ml) and cultured for 24 hr. Inflammation-related elements in the supernatant were measured using enzyme-linked immunosorbent assay (ELISA) kits, and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in the cells was analyzed by Western blotting. The HKSMM50 lowered iNOS and COX-2 protein expressions, and nuclear factor-kappa B (NF-κB), nitric oxide (NO) and prostaglandin E₂ (PGE₂) contents in a concentration-dependent manner as compared to LPS treatment. Similarly, the HKSMM50 lowered the content of pro-inflammatory cytokines interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and interleukin-6 (IL-6) contents and increased the activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). The efficacy of the AHCC treatment was similar to that of the HKSSM50 treatments. These results indicate that HKSMM50 showed an anti-inflammatory effect in LPS-treated RAW 264.7 cells by down-regulation of NF-κB signaling and suggest that HKSMM could be used as a health functional food ingredient to help improve immune function.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.979-986
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• 2022

In this study, the virtual exhibition using 3D architectural space was analyzed in terms of the viewer's experience. For this purpose, the analysis items of the virtual architectural space include whether the actual architectural space is reproduced, the introduction of surreal elements, the degree of freedom of movement and circulation, the level of photorealism of spatial expression, the level of reproduction of the exhibits and information provision method, and the interaction with other participants. Six virtual exhibition projects designed by a well-known architect were selected and analyzed. Three directions were found through the analysis. First, even when designing a virtual exhibition space with a high degree of freedom, there is a tendency to present a familiar architectural environment. Second, the current method of creating a virtual architectural space is that the method using a 360-degree rendering image and the method using a game engine coexist with pros and cons. Third, the interaction between participants in the virtual exhibition is implemented only by using a game engine. It is expected that the virtual space production environment using the game engine to be developed will become more advantageous in the future.