• Title/Summary/Keyword: electrical manipulation

Search Result 117, Processing Time 0.023 seconds

Real-Time Force Sensing in the Envelope of Zebrafish Egg during Micropipette Penetration

  • Yun, Seok;Kim, Deok-Ho;Kim, Byung-Kyu;Lee, Sang-Ho;Park, Gwi-Tae
    • 제어로봇시스템학회:학술대회논문집
    • /
    • 2003.10a
    • /
    • pp.2451-2456
    • /
    • 2003
  • In biological cell manipulation, manual thrust or penetration of an injection pipette into an egg is currently performed by a skilled operator, relying only on visual feedback information. Massive load of various micro injection of either genes, fluid or cells in the postgenomic era calls a more reliable and automatic micro injection system that can test hundreds of genes or cell types at a single experiment. We initiated to study cellular force sensing in zebrafish eggs as the first step for the development of a more controllable micro injection system by any inexperienced operator. Zebrafish eggs at different developmental stages were collected and an integrated biomanipulation system was employed to measure cellular force during penetrating the egg envelope, the chorion. First of all, the biomanipulation system integrated with cellular force sensing instrument is implemented to measure the penetration force of cell membranes and characterize mechanical properties of zebrafish embryo cells. Furthermore, implementation of cellular force sensing system and calibration are presented. Finally, the cellular force sensing of penetrating cell membranes at each developmental stages was experimentally performed. The results demonstrated that the biomanipulation system with force sensing capability can measure cellular force at real-time while the injection operation is undergoing. The magnitude of the measured force was in the range of several hundreds of uN. The precise real-time measurement should provide the first step forwards for the development of an automatic and reliable injection system of various materials into biological cells.

  • PDF

Epidural Steroid Injection in the Treatment of Cervical Radiculopathy (Cervical Radiculopathy에 대한 경막외 Steroid 주입에 관하여)

  • Choe, Huhn;Han, Young-Jin;Baek, Hae-Jung;Kim, Dong-Chan
    • The Korean Journal of Pain
    • /
    • v.4 no.2
    • /
    • pp.152-156
    • /
    • 1991
  • Cervical radiculopathy has been recognized as a common cause of neck, shoulder, and arm pain. It was conventionally treated with rest, traction, cervical collars, manipulation, transcutaneous electrical nerve stimulation, and nonsteroidal anti-inflammatory drugs with varying results. Surgery has not always been successful so the idea of epidural steroid injection was developed as in the case of lumbar epidural injections, for the treatment of low back pain and sciatica. Thirty one patients with evidence of cervical radiculopathy were treated with injections of triamcinolone acetate into the cervical epidural space. The effect of the steroid injection could be evaluated in twenty one patients who received epidural injections more than twice. No improvement was seen in 6 patients and 15 patients showed good to excellent pain relief for a week to over 6 months. Epidural steroid injection seems to have a positive role in the treatment of cervical radiculopathy.

  • PDF

Detection of Facial Feature Regionsby Manipulation of DCT's Coefficients (DCT 계수를 이용한 얼굴 특징 영역의 검출)

  • Lee, Boo-Hyung;Ryu, Jang-Ryeol
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.8 no.2
    • /
    • pp.267-272
    • /
    • 2007
  • This paper proposes a new approach fur the detection of facial feature regions using the characteristic of DCT(discrete cosine transformation) thatconcentrates the energy of an image into lower frequency coefficients. Since the facial features are pertained to relatively high frequency in a face image, the inverse DCT after removing the DCT's coefficients corresponding to the lower frequencies generates the image where the facial feature regions are emphasized. Thus the facial regions can be easily segmented from the inversed image using any differential operator. In the segmented region, facial features can be found using face template. The proposed algorithm has been tested with the image MIT's CBCL DB and the Yale facedatabase B. The experimental results have shown superior performance under the variations of image size and lighting condition.

  • PDF

An Energy-Efficient Clustering Design Apply Security Method in Ubiquitous Sensor Networks (USN에서 보안을 적용한 에너지 효율적 클러스터링 설계)

  • Nam, Do-Hyun;Min, Hong-Ki
    • Journal of IKEEE
    • /
    • v.11 no.4
    • /
    • pp.205-212
    • /
    • 2007
  • The ubiquitous sensor network consists of micro sensors with wireless communication capabilities. Compared to wired communication, wireless communication is more subject to eavesdropping as well as data variation and manipulation. Accordingly, there must be efforts to secure the information delivered over the sensor network. Providing security to the sensor network, however, requires additional energy consumption, which is an important issue since energy transformation is difficult to implement in a sensor network. This paper proposes a routing mechanism based on the energy-efficient cluster that features security functions capable of safely processing the data acquired from the sensor network. The proposed algorithm reduces energy consumption by fixing the clusters formed at the initial stage and using the pre-distribution scheme so that the cluster and node keys generated and exchanged at the initial stage are not re-generated or re-exchanged. Simulation experiments confirmed that the proposed approach reduces energy consumption compared to implementing security measures to the conventional cluster-based routing mechanism.

  • PDF

Controlled Synthesis of Single-Walled Carbon Nanotubes

  • Park, Chong-Yun
    • Proceedings of the Korean Vacuum Society Conference
    • /
    • 2011.02a
    • /
    • pp.2-2
    • /
    • 2011
  • Single-walled carbon nanotubes (SWNTs) have been considered as a promising candidate for nextgeneration electronics due to its extraordinary electrical properties associated with one-dimensional structure. Since diversity in electronic structure depends on geometrical features, the major concern has been focused on obtaining the diameter, chirality, and density controlled SWNTs. Despite huge efforts, the controlled synthesis of SWNTs has not been achieved. There have been various approaches to synthesize controlled SWNTs by preparation of homogeneously sized catalyst because the SWNTs diameter highly depends on catalyst nanoparticles size. In this study, geometrically controlled SWNTs were synthesized using designed catalytic layers: (a) morphologically modified Al2O3 supporting layer (Fe/Al2O3/Si), (b) Mo capping layer (Mo/Fe/Al/Si), and (c) heat-driven diffusion and subsequent evaporation process of Fe catalytic nanoparticles (Al2O3/Fe/Al2O3/Si). These results clearly revealed that (a) the grain diameter and RMS roughness of Al2O3 supporting layer play a key role as a diffusion barrier for obtaining Fe nanoparticles with a uniform and small size, (b) a density and diameter of SWNTs can be simultaneously controlled by adjusting a thickness of Mo capping layer on Fe catalytic layer, and (c) SWNTs diameter was successfully controlled within a few A scale even with its fine distribution. This precise control results in bandgap manipulation of the semiconducting SWNTs, determined by direct comparison of Raman spectra and theory of extended tight binding Kataura plot. We suggest that these results provide a simple and possible way for the direct growth of diameter, density, and bandgap controlled SWNTs by precise controlling the formation of catalytic films, which will be in demand for future electronic applications.

  • PDF

The Effects of Lumbar Stabilizing Exercise on the Functional Recovery and the Range of Motion of Low Back Pain Patients (요부 안정화 운동이 요통환자의 기능회복과 가동범위에 미치는 영향)

  • Jung Yeon-Woo;Bae Sung-Soo
    • The Journal of Korean Physical Therapy
    • /
    • v.16 no.1
    • /
    • pp.157-182
    • /
    • 2004
  • The purpose of this study was to evaluate effects of lumbar stabilizing exercise on the functional recovery and the range of motion of low back pain patients. The subjects were consisted of sixty patients who had non specific chronic low back pain(32 females. 28 males; mean aged 37.3) from 19 to 65 years of age(mean age : 37.3). All subjects randomly assigned to the lumbar stabilizing exercise group, the modalities treatment group, the manual treatment group. Lumbar stabilizing exercise group received manual treatment with lumbar stabilizing exercise for 30minutes, modalities treatment group received hot pack used thermal therapy for 20minutes and ICT used electrical therapy for 20minutes and US or MWD used deep thermal therapy for 15minutes, manual treatment group received modalities treatment with therapeutic massage for 10minutes and joint mobilization or manipulation for 10minutes per day and three times a week during 4 weeks period. The Multilevel Roland-Morris Disability Questionnaire(MR-MDQ) was used to measure functional disability level. Visual Analogue Scale(VAS) was used to measure subjective pain level. Remodified Schober test(RST) was used to measure forward flexion range of motion of lumbar segment. Finger-to-Floor test(F-T-FT) was used to measure forward flexion range of motion of full spine of low back pain patients. All measurements of each patients were measured at pre-treatment and 4 week post-treatment. The results of this study were summarized as follows : 1. The MR-MDQ of lumbar stabilizing exercise group, modalities treatment group, and manual treatment group was significantly reduced between pre-treatment and post-treatment(p<.05). 2. The VAS of lumbar stabilizing exercise group, modalities treatment group, and manual treatment group was significantly reduced between pre-treatment and post-treatment(p<.05). 3. The RST of lumbar stabilizing exercise group, modalities treatment group, and manual treatment group was significantly reduced between pre-treatment and post-treatment(p<.05). 4. The F-T-FT of lumbar stabilizing exercise group, modalities treatment group, and manual treatment group was significantly reduced between pre-treatment and post-treatment(p<.05). 5. The results of analyzed effects of MR-MDQ, RST, F-T-FT were significantly reduced (p<.05), but VAS wasn't significantly reduced(p>.05) between treatment type of lumbar stabilizing exercise group and modalities treatment group and manual treatment group according to pre-treatment and post-treatment. 6. The results of LSD post-hoc to find difference between treatment type of lumbar stabilizing exercise group and modalities treatment group and manual treatment group according to pre-treatment and post-treatment that MR-MDQ was significantly reduced stabilizing exercise group than modalities treatment group(p<.05), and VAS wasn't significantly reduced all treatment group(p>.05), and RST was significantly reduced stabilizing exercise group than modalities treatment group(p<.05), and F-T-FT was significantly reduced stabilizing exercise group than modalities treatment group and manual treatment group (p<.05).

  • PDF

A Study on the Improvement of Utilization through Recognition of Virtual Training Content Operating Institutions (가상훈련 콘텐츠 운영기관 인식을 통한 활용도 제고방안 연구)

  • Miseok Yang;Chang Heon Oh
    • Journal of Practical Engineering Education
    • /
    • v.14 no.3
    • /
    • pp.479-489
    • /
    • 2022
  • In order to understand how to increase the use of virtual training content at K University's online lifelong education institute, this study examined the use experience, content recognition, field practice replacement, and requirements, focusing on the examples of operating institutions. To this end, 12 institutions that operated virtual training contents distributed by the K University Online Lifelong Education Center in 2020 were selected for in-depth interviews and qualitative analysis was conducted on the interviews of 11 institutions. As a result of the analysis, first, the experience of using the contents of the virtual training operating institution was aimed at changing the educational environment, supplementing theoretical learning, and improving the sense of practice. Second, according to a survey on the recognition of virtual training content, if the importance and utilization of the content are high, it can be replaced by on-site practice in non-face-to-face classes, such as experiences of facilities and equipment, attracting interest and attention. Third, in many cases, the perception of replacement for field practice is not unreasonable to use as a pre-training material for field practice, but it is difficult to replace field practice. Fourth, content quality improvements can be summarized as content quality improvement, content access and manipulation improvement, dedicated device development, training for instructors, and curriculum systematization. Fifth, institutional requirements include improving the quality of virtual training content itself, equipment support, curriculum systemization and characterization, systematic curriculum and detailed content sharing, detailed guidance on using virtual training content, introducing how to use content, and recruiting instructors. This study is meaningful in that it sought ways to improve the utilization of virtual training content based on the perception of virtual training content operating institutions.