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Development of Economical Artificial Diets for Greater Wax Moth, Galleria mellonella (L.) (꿀벌부채명나방[Galleria mellonella (L.)] 사육을 위한 경제적 인공사료 개발)

  • Lee, Seung-Wook;Lee, Dong-Woon;Choo, Ho-Yul
    • Korean journal of applied entomology
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    • v.46 no.3
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    • pp.385-392
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    • 2007
  • This research was carried out to develop economical artificial diets of the greater wax moth, Galleria mellonella (L.). Diets were mixed with malt or sugar instead of whole honey for cheaper ones. fecundity, developmental period, pupation rate, emergence rate and adult longevity of G. mellonella depending on diet were examined. The head width and length of larvae were not significantly different among diets tested. However, the weight of larva was the heaviest, 255.5 mg, at 1/2 honey + 1/2 malt diet and lightest, 144.3 mg, at sugar diet. The weight of pupa was also the heaviest at 1/2 honey + 1/2 malt representing 196.7 mg. Larval period was the shortest at honey diet as 30.9 days but the longest at sugar diet as 36.5 days. Pupation and emergence rates were over 79.3% at all diets. The longevity of mated females was ranged from 6.3 to 8.0 days and those of mated males ranged from 7.9 to 11.2 days. The highest number of eggs, 1269, was obtained at sugar diet. Artificial diets replaced by rice bran, malt, and sucrose reduced costs compared with wheat bran plus honey diet. The cost of rice bran was only 10% of wheat bran. Honey was much more expensive than malt and sugar, that is 6 and 13 times, respectively.

Biodegradation of Abandoned Livestock by Blow Flies (Diptera: Calliphoridae) (검정파리(Calliphoridae)에 의한 폐가축의 분해 촉진)

  • Yun, Ji-Eun;Kang, Gi-Cheol;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.47 no.2
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    • pp.185-188
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    • 2008
  • A possible rapid biodegradation of abandoned livestock was tested by using the dead pig and chicken. The dead pig (36kg) was completely decomposed after 23 days of placement in the open field during June 2007. When the door of a cage in which a dead chicken (3.4kg) was placed was opened, a lot of flies were attracted to the chicken and layed eggs on the chicken. As the result the chicken was decomposed down to 0.6kg after 6 days of placement in the open field, On the other hand when the door was closed, the decomposition was very slow. The chicken weighed 3.0kg even after 6 days. An experiment conducted during October 2007 showed that decomposition speed depended on the number of inoculated flies. When 50 pairs of Lucilia sericata flies were inoculated to 3.2kg chicken, it was decomposed down to 1.0kg after 22 days in the field. However, when 200 female and 100 male flies were inoculated, the 3.4kg chicken was decomposed to 0.8kg after 11 days in the field. A 10,858 pupae (371.2g) was produced from the latter chicken. These pupae may possibly be used as a feed for fish and fowl. From these results it is considered that further research is needed to commercialize the blow flies for the rapid decomposition of an abandoned livestock of diverse size under diverse environment.

Population Fluctuation, Developmental Character of Panonychus citri and Damage Degree as its Control Density on Young Yuzu (Citrus junos) (유자에서 귤응애의 발생소장, 온도별 발육특성 및 방제밀도별 피해정도)

  • 최덕수;김규진
    • Korean journal of applied entomology
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    • v.37 no.2
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    • pp.193-198
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    • 1998
  • Experiments were conducted to investigate the population fluctuation, developmental periods, fecundity, hatching rate and demage of Citrus red mite (Panonychus citri M.) on Yuzu trees (citrus junos T.) from 1996 to 1997. Citrus red mite occurred from May to November and made two peaks. The first peak was in July to August and the secondary peak was in October. Density of the second peak was higher (9.5 miteslleaf) than that of the first peak. In the constitution rate of each developmental stage of citrus red mite on Yuzu leaves, egg stage occupied 85%. At the four constant temperature (15, 20, 25, 30 + 1$^{\circ}$C, RH 60 + lo%, 14L- IOD) conditions, the developmental period from egg to adult was 41 .l, 15.5, 11.0 and 9.4 days ; Mean longevity of adult was 23.3, 8.3, 6.3, and 6.1 days; Mean number of egg laid per female per day was 1.6, 3.2, 4.5, 4.0 eggs; Mean hatching rate was 66.6, 85.7, 90.7 and 94.7% at above temperature, respectively. When sprayed acaricide at different density of Citrus red mite, the growth of young Yuzu tree were better at low density. Defoliation rate during winter was 13.5, 20.6, 53.1, 72.6% at 4 control density 1 , 3, 6 mites per leaf and uncontrolled (10 ( ). When every time spray acaricide under 3 mites per leaf, defoliation rate during winter suppressed above 50% compare to uncontrol 72.6%.

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Effectiveness of Several New Insecticides Applied on the Water-surface for the Control of the Rice Stem Borer, Chilo suppressalis Walker, in the 2nd Generation (이화기이화명충에 대한 살충제의 수면시용효과비교시험)

  • Paik Woon Hah;Choi Seung Yoon
    • Korean journal of applied entomology
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    • v.3
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    • pp.41-44
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    • 1964
  • 1. This experiment was planned to compare the effectiveness of Diazinon granular and gammadol dust with the low toxic organophosphorus insecticides applied on the water-surface for the control of the rice stem borer, Chilo suppressalis Walker, in the 2 nd generation and to determine the proper timing of those insecticidal applications at Suwon, Korea, in 1964. 2. The hatching larvae from the eggs were inoculated on the rice plants in pots and insecticides were applied on the water-surface of pots at a given day intervals. The dose of insecticides used per pot was calculated from the 120g. as a actual ingredient per 10 a. The number of living larvae was observed at the 20 days later from the inoculation (as shown in Table 1.). 3. As shown in Table 2 and 3, there was highly significance between the chemicals and L.S.D. was 24.22. The best effectiveness was Diazinon granular and 2nd was gamma-dol dust. The organophosphorus emulsion concentrations used in this experiment were less effective than two other insecticides. Although there were some suspicious results according to the day intervals inoculated, the order of effectiveness was Diazinon>gamma-dol>Sumithion>EM>EPN> Cidial as the total moan. 4, As shown in Table 2 and 3, there was no significance between the timing of insecticidal applications, but, m the whole, Diazinon granular was most effective at a given day intervals in this experiment and gamma-dol also was effective at the day Plot inoculated. The proper timing of EPN, EM, and Cidial seemed to be at e day plot inoculated and that of Sumithion at the day plot before inoculation.

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Transmission of Paulownia witches'-broom virus by tobacco leaf bug, Cyrtopeltis tenuis REUTER (담배장님노린재 (Cyrtopeltis tenuis REUTER)에 의한 오동나무천구 소병(도깨비집병) 바이러스의 매개전염)

  • La Y. J.;Pyun B. H.;Shim K. J.
    • Korean journal of applied entomology
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    • v.5_6
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    • pp.1-7
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    • 1968
  • The present investigation was initiated to find out the possible insect vector of the paulownia witches'-broom virus disease. 1. Among a number of piercing-sucking mouth type insects that were feeding on the witches'-broom infected Paulownia leaves, the tobacco leaf bug, Cyrtopeltis tenuis REUTER, was most abundant. Eggs, nymphs and adults of Cyrtopeltis tenuis were observed from the mid-August through the end of October. 2. It was found that Paulownia witches'-broom virus is transmitted by Cyrotopeltis tenuis. 3. On young Pnulownia plants, first symptom appeared twenty days after exposure to virus-bearing colonies of Cyrtopeltis tenuis. Forty days after exposure to these viruliferous insects, the characteristic symptom of Paulownia witches'-broom was apparent. 4. Paulownia witches' -broom virus was also transmitted by Cyrtopeltis tenuis to calendula and morning glory. No app"rent symptom was observed from tomato, carrot. aster, zinnia, cosmos, soybean and plantago at least until sixty days after exposure to the viruliferous colonies of Cyrtopeltis tenuis. 5. Calendula and morning glory appear to be highly suitable test plants for Paulownia witches'-broom vims because of their relatively short incubation period and distinct symptom appearance.

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Evaluation of Fertility of Artificial Induced Gynogenetic Diploid Male in Paralichthys olivaceus (유도된 자성발생성 2배체 숫컷 넙치(Parlichthys olivaceus)의 생식 능력 평가)

  • KIM Bong-Seok;MOON Young Bong;JEONG Chang Hwa;KIM Dong Soo;LEE Young-Don
    • Journal of Aquaculture
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    • v.7 no.3
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    • pp.151-158
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    • 1994
  • To evaluate the reproductive ability of gynogenetic diploid male. Paralichthys olivaceus. histological analysis of testis. cytological analysis of spermatozoa and fertilization test with normal aggs were studied and the results are as follow; The gonads of gynogenetic diploid male were histologically normal. and many spermatozoa were observed in their testis. Number of spermatozoa from the control and gynogenetic diploid male were $2.58\times10^9$ and $2.42\times10^9$ cells per 1 ml of milt. respectively (P> 0.05). Amount of milt per kg body weight from the gynogenetic diploid male was significantly higher (P< 0.01) than that from the control male (8.3ml). Size and morphology from the two experimental groups were not different (P>0.05). More than $80\%$ of fertilization rates and hatching rates were observed when the eggs from the control were fertilized with the gynogenetic diploid male sperms.

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A Survey of Nutrition and Blood Contents of Rural Women in Korea (일부 농촌지역 부인의 영양실태 및 혈액성상에 관한 연구)

  • Lim, Hyeon-Sook;Hwang, Geum-Hee
    • Journal of Nutrition and Health
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    • v.15 no.3
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    • pp.171-180
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    • 1982
  • The nutritional survey and blood sampling was conducted from May 23 to 27, 1971, in order to investigate the state of the nutritional intake in relation to blood contents of rural women. This survey covered a total of 47 women residing in Youngam-gun, Cholla-namdo, Korea. The daily diet of these subjects was observed by 24-hour recall method and weighing method for 3 days and hemoglobin (Hb), hematocrit(Hct), mean corpuscular hemoglobin concentration (MCHC), serum protein, serum albumin, A/G ratio, serum iron, total iron binding capacity (TIBC), transferrin saturation (TS), serum cholesterol and blood specific gravity (SG) were measured. The results were summarized as follows ; The rate of intake of vegetable foods was high, especially cereals, and that of animal foods was low, especially eggs and milk and milk products. The daily intake of niacin was sufficient, and the intakes of energy and iron were also tend to be adequate. On the contrary, the intakes of vitamin A, $B_2$ and C, calcium and protein were insufficient. The mean red blood cell number was $410.8{\pm}26.4{\times}10^{4}/mm^{3},$ the mean values of Hb, Hct and MCHC were $12.5{\pm}0.9gm%$, $37.5{\pm}2.4%$ and $33.2{\pm}0.5%$ thus the anemic prevalences were 21.3%, 12.8% and 93.6% comparing with the anemic criteria of WHO. The levels of serum protein and serum albumin were $7.1{\pm}0.4gm\;%$ and $4.3{\pm}0.3gm\;%$so the A/G ratio was indicated $1.6{\pm}0.2.$. The levels of serum iron and TIBC were $91.9{\pm}27.5{\mu}g/100ml and $297.5{\pm}31.4{\mu}g/100ml,$ so TS was calculated $31.2{\pm}9.7\;%$. The mean value of SG was $1.044{\pm}0.002,$ and the level of serum cholesterol was $164.3{\pm}25.4mg/dl$. In the relation between each of bloood content, Hb showed positive correlation not only with Hct but also protein at the level of significance of 1 per cent and 5 per cent, and protein also showed a positive correlation with serum cholesterol at the 1 per cent level of significance. In the relation between the nutrient intake and blood contents, vitamin C intake showed a positive correlation with Hb, serum protein and serum iron at the level of significance of 1 per cent, iron intake also showed a positive correlation with serum iron and serum cholesterol at the same significant level, and protein intake showed a positive correlation with serum iron at the at the level of significance of 5 per cent.

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Development of a Food Frequency Questionnaire for the Korea National Health and Nutrition Examination Survey: Data from the Fourth Korea National Health and Nutrition Examination Survey (KNHANES IV) (국민건강영양조사 식품섭취빈도조사지 개발: 국민건강영양조사 제4기 (2007-2009) 자료 이용)

  • Yun, Sung Ha;Shim, Jee-Seon;Kweon, Sanghui;Oh, Kyungwon
    • Journal of Nutrition and Health
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    • v.46 no.2
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    • pp.186-196
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    • 2013
  • The objective of this study was to develop a semi-quantitative food frequency questionnaire (FFQ) for an adult population for use in the Korea National Health and Nutrition Examination Survey (KNHANES). The 24-hour recall data for 2,939 subjects aged 19 years and over from the fourth KNHANES first year (2007) were used to extract the items included in the questionnaire. The FFQ items were developed by selection of major dishes based on the cumulative contribution rate, between-person variability based on the cumulative R2 of energy and 14 nutrients (carbohydrate, protein, fat, crude fiber, calcium, phosphorous, iron, sodium, potassium, vitamin A, thiamin, riboflavin, niacin, and vitamin C) and the number of consumers of each dish. In addition, the FFQ items were revised with analysis of 24-hour recall data of the fourth KNHANES second and third year (2008, 2009). Finally, 112 items were included in the FFQ and grouped as follows: rice (5 items), noodles and dumplings (6), breads and rice cakes (8), soups and stews (12), soybeans, eggs, meat and fish (23), vegetables, seaweed and potatoes (27), milk and dairy products (4), fruits (13), beverages (5), snacks (6) and alcoholic beverages (3). The food items of FFQ accounted for an average of 87.0% of energy and 14 nutrient intakes and also accounted for 81.7% of the between-person variability. The frequency range of the FFQ items was classified into nine categories (never or seldom, once per month, 2-3 times per month, once per week, 2-4 times per week, 5-6 times per week, once per day, twice per day and three times per day) and the portion size was divided into three categories (small, medium and large). We expect that this developed dish-based FFQ could be used in assessment of longterm dietary intakes of Korean adults.

Spawning Period and Sex Inversion of Black Seabream Acanthopagrus schlegelii Collected off the Coast of Tongyeong, Korea (통영 연안에서 채집된 감성돔(Acanthopagrus schlegelii)의 산란시기와 성전환)

  • Lee, Hae Won;Jeong, Jae Mook;Yu, Hyo Jae;Hwang, Kang Seok;Oh, Si Eun;Song, Se Hyun
    • Korean Journal of Ichthyology
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    • v.33 no.1
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    • pp.37-42
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    • 2021
  • This study sought to reveal spawning period and sex inversion of black seabream, Acanthopagrus schlegelii collected off the coast of Tongyeong, Korea. The monthly gonodosomatic index (GSI) showed the same pattern of between both sexes, and it was estimated that black seabream spawned once a year (between April and May). Histological examination revealed the transition from male to female sex, size of fork length bisexual gonad with ovarian (primary hermaphrodite; progress from male to female, MF) was seen at 20.5 to 39.6 cm and bisexual gonad with testicular (post hermaphrodite; female development and Male degeneration, FM) was seen at 26.4 to 50.2 cm. The egg diameters was in the range of 0.02 to 0.60 mm, with the smallest average egg diameter of 0.09 mm in February, the highest average egg diameters of 0.43 mm in August, and the range of fecundity was number of eggs 277,148 (33.9 cm) to 2,772,421 (34.1 cm).

Developmental and ovipositional charcteristics of Tenodera aridifolia (Mantodea: Mantidae) (왕사마귀(Tenodera aridifolia)의 발육 및 산란 특성)

  • Moon, Hyung cheol;Lim, Ju Rak;Kwon, Suk Ju;So, Sun Young;Cheon, Hyung Kwon
    • Journal of Sericultural and Entomological Science
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    • v.55 no.1
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    • pp.1-5
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    • 2019
  • This study was carried out to investigate the development and oviposition characteristics of Tenodera aridifolia. The nymphs of T. aridifolia grew to adults through 6 and 7 nymphal instars. The percentage of 7 nymphal instars (7-instar type) at 25, 28, and $30^{\circ}C$ were 77.3, 76.2, and 73.5%, respectively. In the 7 instars type, total nymphal periods at 25, 28, and $30^{\circ}C$ were 71.9, 53.5, and 54.7 days, respectively. And, there was no difference in deveolpmental periods between 6- and 7-instar types at all temperatures. Egg periods were 41.3 days at $25^{\circ}C$. Adult longevity of females were 82.0 days, deposited average 3.2 oothecae at intervals of 11.7~18.7 days at $28^{\circ}C$ during her life span. The first ootheca was the largest and $40.5mm{\times}22.0mm{\times}17.3mm$ in size. The number of average eggs per oothecae was 178.6 at $28^{\circ}C$.