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Occurrence of Unfertilized Eggs in the Mulberry Silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae)

  • Saheb, N.M. Biram;Singh, Tribhuwan;Saratchandra, Beera
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.1
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    • pp.1-7
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    • 2009
  • Quality of seed in the mulberry silkworm, Bombyx mori (L.) is determined by many important factors, wherein unfertilized eggs play an important role. Unfertilization of eggs are caused by several reasons such as, abnormality in the sexual organs of the male and female, abnormal development of the micropylar end of the egg, unfavorable environmental conditions during spinning, cocoon preservation, imperfect handling of moths, mating, ovipostion, cold storing of pupae / moths and indiscriminate use of male moths etc. Though the presence of unfertilized eggs would in no way affect the fertilized ones and their quality directly, the frequency of their occurrence underrates the quality and brings down the hatching percentage. Lower the occurrence of unfertilized eggs, higher is the rating of seed quality. Of the various intrinsic and extrinsic factors and events involved in egg deposition of an adult silk moth, mating is an instinct and a biological obligation for the ultimate perpetuation of the species and a must to provide stimulus for oogenesis and bring about biochemical changes in the spermatophore of the silkworm in order to ensure the presence of sufficient number of normal sperms and testicular fluid in the female reproductive organ, activating ovulation and accelerating oviposition behavior and egg deposition. An attempt has been made in this article to briefly elucidate the characteristics of unfertilized eggs, causes of their occurrence and its impact as well as the significance in silkworm seed production.

Effect of Microsporidian Infection on Reproductive Potentiality on Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) in Different Seasons

  • Chakrabarti, Satadal;Manna, Buddhadeb
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.1
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    • pp.157-163
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    • 2008
  • Infection of pathogenic microsporidia, Nosema bombycis and Nosema mylitta (Chakrabarti and Manna, 2006) decreased egg production, fecundity, hatching % and increased sterile eggs in heavily infected mulberry silkmoth, Bombyx mori L. On an average a disease free moth laid upto 442.67 eggs with high hatching % (99.53) and less sterile eggs ($0.47{\sim}2.00%$). While an infected moth laid less number of eggs ($7.00{\sim}412.00$) with low hatching % ($32.437{\sim}98.643$) and high sterile eggs ($2.143{\sim}129.571$). Fecundity of disease free laying was highest (468.714) during season-1 then gradually decreased during season- 2 (414.000) to season- 3 (404.285). But fecundity of an infected laying was highest during season-2 and hatched eggs were lowest during season-2. Higher inoculums concentration of N. mylitta infected to 5th stage larva of mulberry silkworm drastically decreased the fecundity in season - 3 and lower inoculums concentration of N. bombycis decreased the fecundity in season-1 and 3. Season-3 was most effective season to decrease the fecundity and increase sterile eggs when both temperature and humidity were fluctuated from the optimum level.

Variation in the reproductive rate of Trypoxylus dichotomus (Dymastinae: Sacarabaeidae: Coleoptera) by partition

  • Seo, Won-Jun;Park, Sang-Eun;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.47 no.4
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    • pp.867-884
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    • 2020
  • Trypoxylus dichotomus is highly utilized in the domestic insect industry and bred at many farms nationwide. Recently, the number of farmers has been increasing due to the expansion of its use in food and medicine. However, the traditional breeding methods have high economic costs, which include relatively large breeding boxes, large amounts of sawdust and a 2 to 1 ratio (or higher) of female to male. Thus, it is essential to develop a cost-effective breeding method. In this study, a breeding cage with a partition was designed and used to investigate the reproduction rate of T. dichotomus with only a single pair of male and female insect. According to the investigation, the average number of eggs laid in the un-partitioned breeding cage was 93.6, and 121.3 eggs were laid in the partitioned breeding cage. This result shows that T. dichotomus lays more eggs in the partitioned breeding cage rather than in the un-partitioned breeding cage. In addition, it was found that T. dichotomus tends to lay more eggs in compressed sawdust, which was harder than the common fermented sawdust. Through a wind-tunnel assay, it was shown that the ovipositing female recognizes the eggs and avoids them to disperse their eggs elsewhere. These results indicate that the partitioned breeding cage may increase the hardness of the sawdust, restrict the adult's movement and provide a new place for oviposition for the female, and therefore results in an increased reproduction rate with less cost.

Seasonal Variation in Species Composition of Ichthyoplankton in Northern Jinhae Bay, Korea (진해만 북부 해역에 분포하는 부유성 난 및 자치어의 종조성 및 양적변동)

  • Han, Kyeong-Ho;Yu, Tae-Sik;Lee, Jin;Lee, Sung-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.1
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    • pp.72-78
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    • 2018
  • Fish eggs and larvae were collected in July and November of 2013 and January and May of 2014 to determine seasonal variation in the species composition of ichtyoplankton. Samples were classified based on morphological characteristics. Fish eggs were identified as belonging to five taxa; the most dominant species of fish eggs was Engraulis japonicus (62.05%) followed by Sardinops melanostictus (21.02%) and Leiognathus nuchalis (13.71%). These three species accounted for 96.8% of the total number of fish eggs collected. Larvae of 17 species in six families, and six orders were collected. The most dominant species of fish larvae was E. japonicus (51.79%), followed by L. nuchalis (12.59%) and Omobranchus elegans (12.08%). These three species accounted for 76.46% of the total number of larvae collected. Jinhae Bay was identified as a major spawning and nursery ground for fish species inhabiting in the south coast of Korea, such as E. japonicus, L. nuchalis, S. melanostictus, and Omobranchus elegans. Therefore, management of marine fish resources in the South Sea should necessarily include the ecological management of Jinhae Bay.

Comparison of Non-surgical and Surgical Recovery of Fertilized Eggs in Superovulated Rabbits (과배란 처리 가토에서 수정란의 비외과적 회수와 외과적 회수의 비교)

  • 심금섭;변태호;이재근
    • Korean Journal of Animal Reproduction
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    • v.8 no.1
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    • pp.16-21
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    • 1984
  • This experiment was carried out to compare with the nonsurgical and surgical recovery of fertilized eggs in super-ovulated rabbits. Sixty-four eggs recovered were transferred to twelve synchronized, pseudopregnant rabbits to test the viability of the eggs by surgical transfer. Each group(I, II, III) received a single subcutaneous injection of 5mg PGF2${\alpha}$/kg B.W. at 24(Group I), 48(Group II) and 72 hours (Group III) after mating, respectivity. After the administration of PGF2${\alpha}$, vaginal washings were conducted at 3, 6, 9, 12 and 24 hrs, and frequency of vaginal washing was 5 times for the each group (I, II, III). In Group (IV, V, Ⅵ), the rabbits were killed to recover the fertilized eggs from the genital tract at 24(Group Ⅵ), 48(Group V) and 72 hours (Group Ⅵ) after mating, respectively. The results obtained were as follows: 1. Of the total eggs, 69.3%, 73.4% and 66.9% were recovered for Group I, II and III, respectively from the vagina within 6 hrs after PGF2${\alpha}$ injection and particularly for Group III. 2. The rates of egg recovery versus the number of corpora lutea were 55 (51.6-60%), 35.8 (24-52.6%), 33.4 (25-47%) and 72 (70.7-73.0)%, 60.3 (50-71.4)%, 449(44.4-45.5)% in Group I, II, III and Group IV, V, Ⅵ, respectively. 3. Most of eggs recovered were one-cell stage in Group Iand Group IV. More than one half of the eggs recovered in Group II and V were over eight-cell stage, and most of the eggs were so in Group III and Ⅵ. 4. When sixty-four eggs recovered between 24 to 72 hours after mating were transferred to pseudopregnant rabbits. Three recipients were pregnant, and the rate of pregnancy was 25%.

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Gonadal Maturation and Artificial Spawning of the Manila Clam, Ruditapes philippinarum (Bivalvia: Veneridae), in Komso Bay, Korea

  • Chung Ee-Yung;Hur Sung Bum;Hur Young-Baek;Lee Jung Sick
    • Fisheries and Aquatic Sciences
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    • v.4 no.4
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    • pp.208-218
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    • 2001
  • We have investigated the gonad index (GI), gonadal development, reproductive cycle, first sexual maturity, sex ratio, the number of spawned eggs and spawning frequency of the Manila clam, Ruditapes philippinarum. Samples were collected from the intertidal zone of Komso Bay, Korea from January to December in 1999. Monthly changes in the gonad index (GI) and condition index showed a similar pattern in the reproductive cycle. The spawning period was once a year between early June and early October, there was a spawning peak between July and August when seawater temperature was over $20^{\circ}C$. The reproductive cycle of this species can be categorized into five successive stages; early active (February to March), late active (April to May), ripe (April to August), partially spawned (June to October), and spent/inactive stage (August to March). Percentages of first sexual maturity of female and male clams of l5.1-20.0mm in shell length were $56.3\%$ and $60.0\%$, respectively, and $100\%$ for the clams >25. mm. The sex ratio of individuals >15.1 mm in shell length was about 1:1 $(\chi^2= 0.02,\;p>0.05)$. Number of the eggs released from each clam by the induction increased as the size of clam in terms of shell length increased. Mean number of the eggs from the second induction of the spawning was $75.35-84.30\%$ $(average\;79.81\%)$ of the number of the eggs released in the first spawning. Our data indicated that R. philippinarum in Komso Bay has one major spawning peak with over two minor spawning, and the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.

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Investigation of Oviposition Characteristics of Protaetia brevitarsis seulensis (2) - Winter Larvae - (흰점박이꽃무지 산란 특성 조사(2) - 월동(동면) 유충을 중심으로 -)

  • Choi, Sung-Up;Choi, In-Hag;Son, Jin-Sung;Chung, Tae-Ho
    • Journal of Environmental Science International
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    • v.29 no.7
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    • pp.789-792
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    • 2020
  • The present study was investigated the oviposition characteristics of Protaetia brevitarsis seulensis with a focus on winter larvae to improve the utilization of insect resources. The average oviposition tendency and average cumulative number of eggs laid during a 7-week period were measured in adults emerging from larvae that had hibernated for 4 months in five farmhouses. The study covered five zones: Gimje, Jangsu, Wanju, Iksan and Yeoju. The average oviposition tendency over a 7-week period showed increased egg laying between 2 to 3 weeks and 5 to 6 weeks. Overall, it was found that the average cumulative number of eggs laid during the 7-week period was initially low and increased proportionally with time. Our results suggest that using adults that have emerged from hibernated larvae is effective in increasing the oviposition rate and utilization of the insect.

Factors Affecting Acoustic Responses of Egg Shell (난각의 음향반응에 영향을 주는 인자)

  • 조한근;최완규
    • Journal of Biosystems Engineering
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    • v.22 no.1
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    • pp.41-48
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    • 1997
  • A nondestructive quality inspection technique using acoustic impulse response method was studied to investigate the feasibility of egg shell inspection. An experimental system was built to generate impact force, to measure the response signal and to analyze the frequency spectrum. This system includes an impulse generating unit, an egg holding seat, a microphone with preamplifier, and a digital oscilloscope connected to Personal Computer by RS-232C interface. The factors such as impulse generating method, egg holding method, and sensor location were evaluated by analyzing the power spectrum density of the measured signal. The results obtained are summarized as follows : 1. From the sampled eggs, the proper conditions for detecting damaged eggs were found as followings; ceramic for the impact ball material, rubber for egg seat material, 20 degrees for an impact angle of pendulum, 10mm for the distance between egg and sensor, the sharp side for impacting part, and 180 degrees for the location of sensor. 2. Examination of the Fourier transformed analysis in beth normal and damaged eggs revealed that those factors such as the resonant frequency, a number of peak frequencies and the magnitude of power spectrum were important to detect damaged eggs.

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Effects of the first egg day on egg production and egg weight in layer (난용계의 초산일령이 산란능력과 란중에 미치는 영향)

  • 임경순;여정수
    • Korean Journal of Animal Reproduction
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    • v.6 no.1
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    • pp.36-38
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    • 1982
  • For the purpose of identifing the reationship between the first egg day and other economic traits; egg production and egg weight, this experiment was divided 200 birds into four first egg day groups and observed egg number and egg weight every day during 11 months from 2nd December, 1979 to 31st October, 1980 at Animal farm, Yeungnam University. Birds showing 148∼155day in first egg day layed the most eggs among four groups and they produced not only 1.5g heavier eggs than the earlist first egg day group but also similar eggs compared with later matured birds. In egg production above 55g, 148∼155day group showed the best record as a 83.72% of total eggs and also the most egg mass during 11 months.

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Relationship between the abdominal sagging index and the reproductive performance of the Roman goose

  • S. C. Chang;M. J. Lin;L. J. Lin;S. Y. Peng;Tzu Tai Lee
    • Animal Bioscience
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    • v.36 no.4
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    • pp.584-590
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    • 2023
  • Objective: This research aimed to explore the changes in the observed abdominal sagging index (ASI) and reproductive performance of Roman male and female geese during the breeding period. Methods: The 339 six-month-old breeding geese (109 male; 230 female) were used in this study, in which five male and five female geese were slaughtered on a monthly basis to record the ASI. Results: The short diameter of the testes of the male goose when the female goose lays eggs and in the second, third, and fourth months was significantly wider than in the fifth months (19.0, 20.8, 21.4, and 19.6 vs 12.7 and 14.0 mm/bird; p = 0.0105). On the other hand, the testicular weight of the male goose in the second and third months after the female goose lays eggs was significantly higher than that in the second and fifth months after laying (0.33% and 0.37% vs 0.11% and 0.19%; p = 0.0212). During the exploring period, the length and weight of the fallopian tube, the weight of the ovary, the number of follicles in 2 to 3 cm, the number of follicles in 3 to 4 cm, the fallopian tube weight in the carcass weight percentage, and the ovary weight in the carcass weight percentage all demonstrated a significant curve response. Further, female ASI was positively correlated with reproductive tract length (r = 0.815; p<0.05) and egg production per female (r = 0.790; p<0.05). Conclusion: The ASI classification method is more objective and easy to distinguish. This scoring method has a high correlation with the number of eggs laid by each goose and the length of the reproductive tract, inferring that the goose observation could take advantage of ASI during egg-laying and can predict the reproductive system development during the laying period and determine when the breeding goose begins to lay eggs.