• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.52-52
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• 2019

Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.

• 한국환경과학회지
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• 제26권7호
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• pp.869-874
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• 2017

This study was conducted to investigate the effects of dietary supplementation of various types of fermented red ginseng marc with red koji to laying hens on eggshell and egg yolk quality characteristics. A total of 240 Hy-line Brown laying hens (40 wk of age) were randomly allotted to 24 pens (6 replicates per treatment and 10 laying hens per replicate). Experimental diets consisted of 4 treatments containing basal diet (control), 1% fermented red ginseng marc powder with red koji, 1% fermented red ginseng marc pelleted with red koji and 1% fermented red ginseng marc coated with red koji. During the 8-wk feeding trial, there were no significant differences in eggshell strength, eggshell thickness and eggshell color among the treatments, except for eggshell strength at 4 and 8 wk and eggshell thickness at 0 wk. In addition, no differences in egg yolk color and egg yolk index were found for all treatments throughout the 8-wk feeding period, except for egg yolk color at 0 wk. Thus, using various types of fermented red ginseng marc with red koji to laying hens did not improve eggshell and egg yolk quality characteristics.

• 한국발생생물학회지:발생과생식
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• 제18권1호
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• pp.25-31
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• 2014

This study was investigated spawning behavior, structure of egg masses and egg development in Aplysia kurodai inhabiting the coastal waters of Jeju Island, Korea. The mating and courtship behavior of A. kurodai occurred in the form of unilateral copulating with chain formation. In chain copulation, only the first animal acted as a female; the second and succeeding animals acted as males (sperm donors) to the animals in front and as females to the animals behind. The fertilized eggs were packaged in capsules that are embedded in jelly to form a cylindrical string called an egg masses. The number of capsule per cm of the egg masses was 55 to 60 capsules and each capsule within the egg masses held 15 to 25 eggs. After spawning, the egg masses were bright yellow or orange in color. This egg masses color not changed until embryos developed into trochophore stage. Thereafter, as embryo developed from trochophore stage to veliger stage the egg masses color became brownish. The fertilized eggs were spherical, with a diameter of approximately $80{\pm}1{\mu}m$ at spawning. At 5 to 6 days after spawning, the embryo developed into trochophore stage and began to rotate within the egg capsule. In the trochophore stage, the precursor of the velum, called the prototroch or prevelum, developed. At 10 days after spawning, the prevelum is transformed into the velum, and the trochophore developed into veliger stage. Between 10 to 15 days after spawning, the veligers broke out of the egg capsule, and hatched as free-swimming larvae.

• 한국식품영양과학회지
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• 제26권5호
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• pp.860-865
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• 1997

Investigations were performed on the effects of extraction temperature, pressure, time on solubility and selectivity of egg yolk lipid and cholesterol, and color and fatty acid composition of the residue in supercritical carbon dioxide(SC-Co$_2$) extraction. Lipid and cholesterol solubility increased as the increase of Co$_2$ density and was found to strongly depend on the extraction pressure rather than the extraction temperature. The relative concentration of cholesterol in the extract increased with an increase in temperature and decreased with an increase in pressure and extraction time. extraction of dried eg yolk for 3hr at 4$0^{\circ}C$/276 bar removed 46.1% of cholesterol from the residual egg yolk with a yield of 63.2%. SC-Co$_2$ extraction produced a lighter color egg yolk with less redness and yellowness. As the extraction time increased, the resultant residual egg yolk became more saturated with fatty acids. SC-$CO_2$ extraction offers a safe, natural method for removing cholesterol from dried egg yolk.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.84-86
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• 2003

An experiment was conducted to determine the effects of dietary supplementation of yeast Rhodotourula glutinis(Glutincus) on the performance and egg quality of layers. A total of 360 ISA Brown layers of 72 weeks old were assigned to one of the following 6 diets ：control, 25 ppm ${\beta}$-carotene. 0.5％, 1％, 2％ and 4％ Glutinicus supplemented diet. Each treatment was replicated three times with 20 birds housed in 2 birds cage units. Glutinicus supplemented at the level of 0.5％ significantly(p＜0.05) improved egg production and FCR. Soft and broken egge production rate decreased as the level of Glutinicus supplementation increased. Eggshell was highest in Glutinicus 4％ supplementation. Egg yolk color increased linearly as the level of Glutinicus supplementation increased up to 2％ of the diet. It was concluded that Glutinicus supplementation at the level of 0.5％ can be recommended for the improvement of egg production while 2.0％ can be recommended for egg yolk pigmentation.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1605-1610
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• 2012

Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk color of laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown laying hens were fed one of seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 or 9.6 ppm red pepper pigment or 0.3 ppm carophyll red. Each diet was fed to three replicate batteries of hens with each battery consisting of a row of five cages of hens with two hens per cage (n = 3). In Exp. 2, 180, thirty-wk old, Hyline Brown laying hens, housed similarly to those in Exp. 1, were fed an unsupplemented basal diet as well as treatments in which the basal diet was supplemented with 0.8% red pepper powder processed in a laboratory blender to an average particle size of $300{\mu}m$, 0.8% red pepper powder processed as a super fine powder with a vibrational mill ($44{\mu}m$) and finally 0.8% red pepper powder processed as a super fine powder with a vibrational mill but mixed with 5% $Na_2CO_3$ either before or after grinding. A diet supplemented with 0.3 ppm carophyll red pigment was also included (n = 3). In both experiments, hens were fed the red pepper powder or pigment for 14 days. After feeding of the powder or pigment was terminated, all hens were fed the basal diet for eight more days to determine if the dietary treatments had any residual effects. In Exp. 1, there were no differences in egg-laying performance, feed consumption or feed conversion ratio due to inclusion of red pepper pigment in the diet. Average egg weight was higher (p<0.05) for birds fed 1.2, 2.4 or 9.6 ppm red pepper pigment than for birds fed the diet containing 0.3 ppm red pepper pigment. On d 14, egg color scores increased linearly as the level of red pepper pigment in the diet increased. In Exp. 2, feeding red pepper powder did not affect egg-laying performance, feed consumption or feed conversion ratio (p>0.05). However, compared with the control group, supplementation with all of the red pepper powder treatments increased egg weight (p<0.05). All the red pepper powder treatments also increased (p<0.05) the yolk color score compared with the control. The results of the present study suggest that both red pepper powder and pigment are effective feed additives for improving egg yolk color for laying hens.

• 한국가금학회지
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• 제39권1호
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• pp.27-32
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• 2012

This experiment was conducted to investigate the dietary effects of turmeric powder (TP) on laying performances, egg qualities and its transfer into eggs. A total of two hundred, 60-wk-old, Lohmann Brown layers were divided into 4 groups, placed in to 5 replicates per group (10 layers each) and fed each one of four diets containing 0% TP (control), 0.10, 0.25 or 0.50% TP, respectively, for 7 wks. Egg production in the all groups fed diets containing TP were significantly higher than that in control (P<0.05). No differences in feed intakes, egg and eggshell qualities were observed among the treatments, but Roche color fan number (yolk color) in group fed diet with 0.5% TP was significantly higher than in control (P<0.05). In the groups fed diets containing TP, Haugh units after 2 wk of storage were significantly higher than that of control (P<0.05). The curcumin content of egg yolk in the groups fed diet containing 0.50% TP was significantly higher than those in the other groups (P<0.05). The cholesterol levels of egg yolk were not influenced by dietary TP. It was concluded that dietary TP was effective in improving laying performance and internal egg qualities. TP can be also used as a feed additive for the production of value-enhanced eggs with increased yolk color and curcumin content.

• 한국축산식품학회지
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• 제22권3호
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• pp.252-258
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• 2002

본 실험은 CLA급여수준과 기간이 계란의 품질에 미치는 영향에 대하여 연구하였다. 계란의 무게는 CLA 급여에 따른 유의적인 차이를 나타내지 않았으며, 난백의 높이는 급여 7주에서 CLA 급여구가 유의적(P<0.05)으로 낮은 값을 나타내었다. Haugh unit는 CLA 급여에 따른 유의적인 차이는 나타나지 않았다. 난황내 CLA축적량은 급여수준이 높을수록 유의적(P4O.05)으로 높은 축적량을 나타내었다. 그러나 저장 2주 이후에는 증가폭이 크지 않았다. CLA급여에 의한 난황내 지방산 조성을 보면 포화지방산이 증가되고 불포화 지방산이 감소하였는데 포화지방산인 palmitic acid의 증가와 불포화지방산인 oleic acid의 증가가 주요 원인인 것으로 사료된다. 난황색 (Roche)은 CLA 급여에 의해 유의적(P<0.05)으로 높게 나타났으며, 난황의 경도 또한 CLA급여에 의해 유의적(P<0.05)으로 높게 나타났다. 이와 같은 결과는 CLA 급여가 계란의 이화학적인 성질에 영향을 미친다는 것을 알 수있다. 그러나 CLA급여에 의한 계란 품질의 변화가 소비자의 관능적 특성에 미치는 영향에 관해서는 많은 연구가 필요할 것으로 사료된다.

• 한국가금학회지
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• 제49권2호
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• pp.53-60
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• 2022

계란의 내부 및 외부의 품질을 나타내는 요소들 사이의 상관 관계를 분석하여, 할란 검사를 통하지 않고도 계란 품질을 예측하는데 적합한 지표가 있는지 알아보기 위한 실험을 수행하였다. 케이지 계사에서 사육 중인 27주령의 하이라인 브라운 산란계 동일 계군이 같은 날에 산란한 180개의 계란을 수집하였다. 분석 항목으로는 호우유닛, 농후난백높이, 파각 강도, 난각 두께, 난각 색(CIE L^*, CIE a^*, CIE b^*, 반사율), 계란의 중량, 계란의 장경, 계란의 폭, 난형 지수 및 난황색 등 총 13가지의 지표를 조사하였다. 피어슨 상관 계수(r)를 이용하여, 각 품질 지표 사이의 선형 관계성을 평가하였다. 호우유닛과 농후난백높이, 난각색 CIE L^*값과 반사율, 그리고 계란 중량과 계란의 폭 사이에서 매우 강한 정의 상관 관계가 분석되었다(r=0.975, r=0.973, r=0.814, P<0.001). 계란의 중량과 계란의 장경, 난각색 CIE L^*값과 난각색 CIE a^*값, 난각색 CIE a^*값와 반사율, 그리고 난형 지수와 계란의 장경 사이에는 높은 상관 관계가 나타났다(r=0.703, r=-0.726, r=-0.681, r=-0.681, P<0.001). 난각의 파각 강도와 난각의 두께, 난각색 CIE a^*값과 난각색 CIE b^*, 난형 지수와 계란의 폭 사이에는 중간 정도의 상관 관계가 나타났다(r = 0.417, r = 0.590, r = 0.405, P<0.001). 난각색 CIE a^*값은 호우유닛, 농후난백높이와 약한 정의 상관 관계가 있었으며(r=0.217, r=0.202, P<0.01), 난각색 CIE L^*값과 파각 강도 사이에는 약한 부의 상관 관계가 나타났다(r=-0.236, P<0.01). 난각색 CIE b^*값은 난각 두께와 약한 부의 상관 관계를 보였고, 난각색 반사율은 파각 강도 및 난각 두께와 약한 부의 상관 관계에 있는 것으로 나타났다(r=-0.225, r=-0.249, r=-0.222, P<0.01). 그 외 나머지 품질 지표들 간의 상관성은 통계적으로 유의하지 않거나 관계가 없는 것으로 나타났다. 갈색란의 난각색은 반사율만으로 표현하는 것 보다, CIE L^*값, CIE a^*값과 CIE b^*값을 함께 적용했을 때 갈색의 정도를 더 구체적으로 표현한다. 결론적으로 본 연구를 바탕으로 갈색란의 난각색 CIE L^*a^*b^*값은 계란의 품질 지표인 호우유닛, 농후난백높이, 난각의 파각 강도와 두께를 간접적으로 예측하는데 사용할 수 있을 것으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.1015-1020
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• 2012

An experiment was conducted to evaluate the effects of bacteriophage supplementation on egg performance, egg quality, excreta microflora, and moisture content in laying hens. A total of 288 Hy-line brown commercial laying hens (36-wk-old) were randomly allotted to 4 treatments in this 6-wk trial and dietary treatments included: i) CON, basal diet; ii) T1, CON+0.020% bacteriophage; iii) T2, CON+0.035% bacteriophage; iv) T3, CON+0.050% bacteriophage. There were 6 replicates for each treatment with 6 adjacent cages (2 hens/cage). Laying hens in T2 and T3 treatments had higher (p<0.05) egg production than those in CON and T1 treatments during wk 0 to 3. In addition, egg production in T1, T2, and T3 treatments was increased (p<0.05) compared with that in CON treatment during wk 4 to 6. At wk 4 and 5, birds in T2 group had higher (p<0.05) HU than those in CON. In addition, at wk 5 and 6, HU in birds fed T1 and T3 diets was greater (p<0.05) than those fed CON diet. E. coli and Salmonella spp. concentrations in excreta were decreased (p<0.05) by T1, T2, and T3 treatments. However, egg weight, egg shell color, yolk height, yolk color unit, egg shell strength, egg shell thickness, egg gravity, and excreta moisture content were not influenced by dietary treatments during the entire experimental period. In conclusion, bacteriophage supplementation has beneficial effects on egg production, egg albumen, and excreta microflora concentration in laying hens.