• Title/Summary/Keyword: effective microorganisms

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Some Problems of Sikhye Production and An Improvement Method of Sikhye Quality (식혜산업의 문제점과 품질 향상방안)

  • 안용근;이석건
    • The Korean Journal of Food And Nutrition
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    • v.9 no.1
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    • pp.45-51
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    • 1996
  • Korean traditional Sikhye is made from rice and malt, and It's main product Is maltose. However commercial Sikhye differs from traditional Sikhye because it's main component is sucrose. Sikhye industry faces many problems such as contamination of malt with microorganisms, low amylase activity of malt and technical difficulties. There is no commercial Sikhye which is only using rice and malt by these reasons. To produce the traditional Sikhye free from these problems, it is necessary to restrict the microorganisms of malt and to standardize the amylase activity of malt. In addition, the Introduction of effective control and sanitaric process is required. In Sikhye production. if $\beta$-amylase and isoamylase or pullulanase were added, starch could be saccharified 100% as maltose. Accordingly, this method brings us the low cost of Sikhye.

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A Study on the Effects of Fumigation on Excavated Costumes (출토복식유물에 대한 훈증소독의 영향에 관한 연구)

  • 채옥자;박성실;안춘순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.5
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    • pp.668-677
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    • 2004
  • This study examines the effects and influences of fumigation on the antimicrobial treatment of excavated dresses in two different museum settings using chemical composite of Methyl Bromide and Ethylene Oxide. The microbial test indicated that the prewashed samples contained Micrococcus, Bacillus, Staphylococcus, and some unidentified fungi and that the total number of microbes decreased after washing. While it was observed that most germs were removed immediately after fumigation, Bacillus was still found and its number even increased. After 5 months, the number of microbes found in Museum A was smaller than Museum B, which shows that Museum A equipped with better environment control facility was superior to Museum. B in terms of exhibition and preservation of dresses against organic damages. In addition, the result indicates that fumigation is an effective measure against the growth and reproduction of microorganisms, considering that the number of microorganisms decreased after fumigation. The color of test sample showed immediate change after fumigation but color difference became stabilized and decreased with the passage of time. The test samples from both museums showed similar patterns.

Microbial change of pork carcass during processing in small size slaughterhouse (소규모 돼지도축공정에서 도체오염 미생물의 변화)

  • 홍종해;이경환;이성모
    • Korean Journal of Veterinary Service
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    • v.25 no.1
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    • pp.31-37
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    • 2002
  • Major hazards existed in slaughterhouse are pathogenic microorganisms originated from intestinal microflora of slaughtered animals. This study was intended for the identification of microbial contamination sources during pork slaughtering in small plants. Total aerobic bacteria, Coliform group, Salmonella spp, Listeria monocytogenes, and Campylobacter jejuni/coli were isolated from the surface sample of pork carcasses. Contamination level among different sampling points of ham, belly and neck did not showed statistical differences. Therefore, the mixed sampling from belly and neck of carcass could be effective for microbiological monitoring. Isolation rates of pathogenic microorganisms showed Salmonella spp 20.9%, Listeria monocytogenes 10.5%, and Campylobacter jejuni/coli 8.1% from 296 sampling points. High prevalence rate of Salmonella spp indicated that the contamination of intestinal microflora occurred due to unsanitary processing control, which required HACCP system in small plants. It was recommended that the prerequisite program should be a key factor for a successful HACCP system implementation especially in small size slaughterhouse.

Anaerobic-aerobic granular system for high-strength wastewater treatment in lagoons

  • Hamza, Rania A.;Iorhemen, Oliver T.;Tay, Joo H.
    • Advances in environmental research
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    • v.5 no.3
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    • pp.169-178
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    • 2016
  • This study aimed at determining the treatability of high-strength wastewater (chemical oxygen demand, COD>4000 mg/L) using combined anaerobic-aerobic granular sludge in lagoon systems. The lagoon systems were simulated in laboratory-scale aerated and non-aerated batch processes inoculated with dried granular microorganisms at a dose of 0.4 g/L. In the anaerobic batch, a removal efficiency of 25% was not attained until the 12th day. It took 14 days of aerobic operation to achieve sCOD removal efficiency of 94% at COD:N:P of 100:4:1. The best removal efficiency of sCOD (96%) was achieved in the sequential anaerobic-aerobic batch of 12 days and 2 days, respectively at COD:N:P ratio of 200:4:1. Sequential anaerobic-aerobic treatment can achieve efficient and cost effective treatment for high-strength wastewater in lagoon systems.

Antagonism of Pseudomonas spp. against to Rhizoctonia solani and Pythium spp. (Pseudomonas spp.의 Rhizoctonia solani 및 Pythium spp. 병원균에 대한 길항작용)

  • 주영규;한정훈
    • Asian Journal of Turfgrass Science
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    • v.8 no.1
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    • pp.47-52
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    • 1994
  • Attempts were made to investigate the antagonistic activity of soil borne microorganisms Pseudomonas spp. and Trichoderma spp. against to the pathogens of turf diseases Rhizoctionia solani spp. and Pythiom spp. in vitro by a dual culture bioassay. Inhibition zone between the edge of the my-celium and the margin of each antagonistic bacteria, Pocudontonas, on potato dextrose agar was measured 3 days after incubation at 28˚C. Psudomonas spp. showed relatively high inhibition of mycelium growth of R. solani AG-i and Pythium spp. which cause brown patch and pythium blight, respectively. Antagonistic fungi Trichodenma spp. also showed effective inhibition against mycelium growth of both pathogens, more proper methods of measuring the inhibition effects were required because of fast growth of Trichodenna hypae. Brown patch and pythium blight both, re-quire most higher rate of fungicide use to control in golf curses in Korea. Application of antagon-istic microorganisms are useful as biological resources an approach to sole environmental contamination.

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Biodegradation of and comparison of adaptability to dectergents (미생물에 의한 계면활성제의 분해능과 적응력의 비교)

  • 이혜주;홍순우
    • Korean Journal of Microbiology
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    • v.18 no.4
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    • pp.155-160
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    • 1980
  • Microorgansims utilizing anionic detergent as their carbon and sulfur sources were isolated from soils and sewages. Alkyl benzene sulfonate (Hiti) and sodium dodecyl sulfonate (SDS) were the detergent compound tested. Three of these isolated microorganisms were identified as Pseudomonas spp. and the others asKlbsiella, Enterobacter and Acinetobacter. Biodegradation rate of the detergents and growth rate of Acinetobacter Strain II-8, Pseudomonas strain H-3-1 and 554 among six isolated microorganisms were investigated with colorimetric, warburg manometric, and ultraviolet absorption analyses. By performance of 4 serial successive tranfer to new culture broth for the purpose of adaptation method, ABS and SDS could be degraded to far more than 40%-60% and 70%-75%, respectively. However the employment of nonadaptation method, ABS and SDS were degraded to 30%-45% and 45%-65%, respectively. In another words, detergents degradation ability was increased to a certain extent by successive transfer to the new minimal media. We would conclude that the development of adaptation was effective in the removal of recalcitrant compounds.

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Effect of Microbial Product on Microorganisms in Soil and Growth of Chinese Cabbage (미생물제제 처리가 토양 미생물상의 변화 및 배추의 생육에 미치는 영향)

  • Seok, Woon-Young;Oh, Ju-Sung;Kim, Doh-Hoon;Chung, Won-Bok;Jeong, Soon-Jae
    • Korean Journal of Organic Agriculture
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    • v.12 no.4
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    • pp.399-409
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    • 2004
  • This study was conducted to investigate the effect of different concentrations of microbial products on growth of chinese cabbage and microorganisms in soil. Two different levels of microbial products, such as 50 times and 100 times diluted solutions of chitosan, wood vinegar and EM activity liquid, were treated for foliar application. the results were summarized as follows : Among foliar applications of microbial products, 100 times diluted solution of chitosan was effective on growth of chinese cabbage comparing to other levels of dilutions and untreated control plot. The number of microorganism in the soil tended to increase under the treatment of microbial products compared to control plot. Especially, the numbers of the bacteria and actinomycetes were estimated $73.67{\times}10^3$ CFU/g and $34.00{\times}10^3$ CFU/g, respectively, under the treatment of 100 times diluted solution of chitosan.

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Investigating the Impacts of Various Parameters on Lactic Acid Production; A Review

  • Hub-e-Fatima;Hammad Zia;Muhammad Awais;Hafiz Miqdad Masood;Najaf Ali
    • Korean Chemical Engineering Research
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    • v.62 no.4
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    • pp.281-295
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    • 2024
  • This review examines the effects of different process parameters on the production of lactic acid. Especially focusing on the factors such as, pH, temperature, utilization of fungi, viz., Rhizopus species and selection of carbon and nitrogen sources. The development of lactic acid synthesis is promoted by acidic environment, usually falling within pH < 3.5, which allows optimal lactic acid synthesis. Another important factor is temperature. Strains such as lactobacillus rhamnosus DUT1908, have a high tolerance to temperature as high as 50℃, which allows for effective substrate utilization and high lactic acid yield. This review highlights the need of tailoring these process parameters to the specific characteristics of the biomass and the metabolic pathways of the microorganisms to achieve increased lactic acid production.

Isolation of cellulosic biomass degrading microorganisms from different sources for low cost biofuel production

  • Sheikh, M. Mominul Islam;Kim, Chul-Hwan;Lee, Ji-Yong;Yeasmin, Shabina;Park, Hyeon-Jin;Kim, Gyeong-Chul;Kim, Sung-Ho;Kim, Jae-Won
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2011.04a
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    • pp.81-91
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    • 2011
  • Current fuel ethanol research and development deals with process engineering trends for improving biotechnological production of ethanol. Recently, a large amount of studies regarding the utilization of lignocellulosic biomass as a good feedstock for producing fuel ethanol is being carried out worldwide. The plant biomass is mainly composed of cellulose, hemicellulose and lignin. The main challenge in the conversion of biomass into ethanol is the complex, rigid and harsh structures which require efficient process and cost effective to break down. The isolation of microorganisms is one of the means for obtaining enzymes with properties suitable for industrial applications. For these reasons, crude cultures containing cellulosic biomass degrading microorganisms were isolated from rice field soil, cow farm soil and rotten rice straw from cow farm. Carboxymethyl cellulose (CMC), xylan and Avicel (microcrystalline cellulose) degradation zone of clearance on agar platefrom rice field soil resulted approximately at 25 mm, 24 mm and 22 mm respectively. As for cow farm soil, CMC, xylan and Avicel degradation clearancezone on agar plate resulted around at 24mm, 23mm and 21 mm respectively. Rotten rice straw from cow farm also resulted for CMC, xylan and Avicel degradation zone almost at 24 mm, 23 mm and 22 mm respectively. The objective of this study is to isolatebiomass degrading microbial strains having good efficiency in cellulose hydrolysis and observed the effects of different substrates (CMC, xylan and Avicel) on the production of cellulase enzymes (endo-glucanase, exo-glucanase, cellobiase, xylanase and avicelase) for producing low cost biofuel from cellulosic materials.

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Quality Properties of Ginseng Chicken Porridge Prepared with Individually Gamma Irradiated Raw Materials

  • Shin, Mee-Hye;Han, In-Jun;Lee, Ju-Woon
    • Food Science of Animal Resources
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    • v.33 no.6
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    • pp.730-736
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    • 2013
  • This study was to establish irradiation process for serving ginseng chicken porridge to immune-compromised patients. Raw chicken, glutinous rice, ginseng, garlic, dried jujube and carrot were used as raw materials for ginseng chicken porridge. The initial level of microorganisms contaminated in raw materials and their predominant species were determined. The level of microorganism detected in raw chicken and in ginseng were 3.4 Log CFU/g and 4.7 Log CFU/g, respectively. Major predominant microorganisms were Pseudomonas fragi in chicken, Enterobactor faecalis in carrot, and Bacillus subtilis in other materials. Chicken and carrot were excluded from irradiation treatment because ordinary thermal treatment can inactivate the microorganisms contaminated in those materials. Five kGy of gamma ray was the effective sterilizing dose required to inactivate B. subtilis in glutinous rice, garlic and jujube, and 10 kGy in ginseng. Ginseng chicken porridge was prepared with each of raw materials gamma-irradiated with the selected sterilizing doses. Control was ginseng chicken porridge prepared with non-irradiated materials. The growth of microorganisms was not observed in the chicken porridge prepared with irradiated raw materials. Sensory results showed that the score of flavor and off-flavor was slightly lower in ginseng chicken porridge prepared with irradiated raw materials than in control. This was considered to be due to the increase of TBARS values by gamma irradiation. However, there was no significant difference on overall acceptance between the porridge prepared with irradiated raw materials and control. The results showed that the individual gamma irradiation of raw materials can be applied to prepare ginseng chicken porridge as meals for the immunocompromised patients.