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Metabolic Engineering of Saccharomyces cerevisiae to Improve Glucan Biosynthesis

  • Zhou, Xing;He, Jing;Wang, Lingling;Wang, Yang;Du, Guocheng;Kang, Zhen
    • Journal of Microbiology and Biotechnology
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    • v.29 no.5
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    • pp.758-764
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    • 2019
  • ${\beta}$-Glucan is a chief structural polymer in the cell wall of yeast. ${\beta}$-Glucan has attracted intensive attention because of its wide applications in health protection and cosmetic areas. In the present study, the ${\beta}$-glucan biosynthesis pathway in S. Cerevisiae was engineered to enhance ${\beta}$-glucan accumulation. A newly identified bacterial ${\beta}-1$, 6-glucan synthase GsmA from Mycoplasma agalactiae was expressed, and increased ${\beta}$-glucan content by 43%. In addition, other pathway enzymes were investigated to direct more metabolic flux towards the building of ${\beta}$-glucan chains. We found that overexpression of Pgm2 (phosphoglucomutase) and Rho1 (a GTPase for activating glucan synthesis) significantly increased ${\beta}$-glucan accumulation. After further optimization of culture conditions, the ${\beta}$-glucan content was increased by 53.1%. This study provides a new approach to enhance ${\beta}$-glucan biosynthesis in Saccharomyces cerevisiae.

The Transformation of the Content and Forms of Education under the Pressure of the COVID-19

  • Bekh, Yuliya;Zhyzhko, Tetiana;Kravchenko, Alla;Kurhan-Bakoveieva, Yana;Kyzymenko, Iryna;Krasilnikova, Olena
    • International Journal of Computer Science & Network Security
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    • v.22 no.10
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    • pp.310-314
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    • 2022
  • Digitalization affects not only the content of education, but also its organization. These processes have ambiguous implications for the positioning of both universities and teaching staff. The necessary competencies are often acquired outside the walls of educational institutions, because educational programs often do not keep pace with the dynamics of technology. The teacher turns from a bearer of transmitted knowledge and skills into a navigator that helps to navigate the knowledge bases. All these issues are especially acute in relation to the reform of the higher education system in the context of pandemic consequences. The article examines and analyzes the features of the transformation of higher education in the context of a pandemic that faced the world in 2020. It has been proved that the processes of changes and transformations that the higher education system is going through both in an individual country and throughout the world should be defined as transformation, not modernization, reform or transformation.

Programming class plan for artificial intelligence education in industrial high school (공업계 고등학교 인공지능교육을 위한 프로그래밍 수업 방안)

  • Kim, Semin;Hong, Ki-Cheon;You, Kangsoo;Lee, Choong Ho
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2022.05a
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    • pp.349-351
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    • 2022
  • In this study, a programming class plan was designed for artificial intelligence education in technical high schools such as specialized high schools and Meister high schools. In elementary and middle schools or general high schools, it is not easy to secure the number of hours, so it is not easy to proceed with a lot of content and in-depth content. In addition, technical high schools have a good environment for nurturing human resources in the field of artificial intelligence. For this purpose, existing researches related to artificial intelligence education were explored, and programming classes related to artificial intelligence education were grafted into the units of the existing engineering high school programming classes.

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The influence of new nurses' knowledge, nursing performance, and educational needs of chemotherapy medication on chemotherapy medication errors (신규간호사의 항암 투약 간호 지식, 수행도 및 교육 요구도가 항암 투약 오류에 미치는 영향)

  • Song, Eon Jeong; Lee, Gyu Young
    • The Journal of Korean Academic Society of Nursing Education
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    • v.29 no.2
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    • pp.115-123
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    • 2023
  • Purpose: This study aimed to identify the factors affecting the chemotherapy medication errors made by new nurses and to use the results as basic data for the development of a chemotherapy medication nursing education program for new nurses. Methods: This cross-sectional study was conducted with 189 new nurses working at a general hospital and a tertiary general hospital in Korea. The data collection period was from January 11 to February 7, 2021. The data collected during this study were analyzed using the IBM SPSS statistics version 25.0 program. Data analysis included descriptive statistics, independent t-test, ANOVA, and logistic regression analysis. Results: One factor influencing chemotherapy medication errors was new nurses' educational needs (odds ratio=.18, p=.005). As educational needs increased, the probability of making errors in medication was reduced by .18. Conclusion: It is necessary to develop a chemotherapy medication education program tailored to the educational needs of new nurses by considering the education period, method, and content, with a focus on the content with high demand from new nurses.

Board Game Design for Disaster Safety Education for Elementary School Students Based on Learning Motivation Theory (학습동기이론 기반의 초등학생 재난안전 교육을 위한 보드게임 설계)

  • Kim Mira;Jung Hyungwon
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.20 no.1
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    • pp.59-74
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    • 2024
  • In order to improve safety consciousness due to the increase in disasters and safety accidents, safety education is necessary to prepare for disasters with interest in safety. This study is a board game design for disaster safety education for elementary school students based on Keller's learning motivation theory. By considering the school safety curriculum and the safety education contents of the School Safety Mutual Aid Association and the Ministry of Public Administration and Security, the content and goals of learning were derived and the order of learning was determined. When designing game content, the fun elements of the game were applied to Keller's learning motivation inducing factors such as attention concentration (A), relevance (R), confidence (C), satisfaction (S), and educational game design elements to induce the achievement of learning goals at the game planning stage. It is expected that the existing safety education focusing on lecture-style and audiovisual will be supplemented and used in the educational field.

Investigating the reassessment opportunity for prospective teachers within their initial required content course

  • Amanda J. Meiners;Angel Luis Figueroa-Rosado
    • Research in Mathematical Education
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    • v.27 no.2
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    • pp.175-193
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    • 2024
  • In K-12 education, reassessment is a common practice, providing students with opportunities to enhance their understanding through low-stakes assignments. However, reassessment is underutilized in higher education, including during the challenges posed by the COVID-19 pandemic. Our study advocates for expanding the use of reassessment in university settings to promote holistic learning and focus on what shifts of change were made by students in an initial mathematics content course as they sought to gain licensure for teaching in a birth (daycare/pre-K setting) to eighth-grade classrooms. Our study took place during COVID-19 semesters and aimed to examine how using a reassessment approach early on in a gateway course for Prospective Teachers (PTs) affected the pass rate of the course. Results showed significant differences between the PTs who engaged with the test recovery and those who did not. We propose recovery opportunities like ours provide the necessary guidance to support early degree necessary classes that are typically gatekeeping and, as another, likely cause too few students within the courses because they were able to advance into the teacher pipeline and out into the field. Future studies may consider how the reassessment could be done more before the official summative assessment of a unit or chapter to continue the shifts in teaching practices and pedagogy that are constant within the K-12 education systems at the university level.

Teachers' Perspectives on Content-based Instruction in English at a Higher Education in Korea

  • Kim, Namsoon
    • English Language & Literature Teaching
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    • v.18 no.1
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    • pp.91-114
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    • 2012
  • The purpose of this study is to investigate teachers' perspectives on content-based instruction (CBI) in English at a higher education in Korea. Based on the assumption that content-based instruction programs could be successful if teachers were actively involved not only in transmitting the content knowledge but also in students' development in the second or foreign language competence, the study explores teachers' treatments of language in relation to the students' language development in CBI classes. Research questions were related to five areas such as (1) the goals of CBI programs, (2) difficulties in CBI classes, (3) the use of native language, (4) teaching strategies and techniques, and (5) factors that affect the success of the CBI programs. Data gathered from 24 college instructors from a large university located in the metropolitan city of Korea. Results of the study indicated that college instructors of CBI programs had keen interest in developing students' language competence, experienced difficulties in designing course syllabus for mixed leveled group of students, rarely used Korean in class and used successful teaching strategies. Also factors needed to improve the CBI programs were recommended at the end of the study. Results of the study implied that teachers needed to be more aware of the students' learning process of English and to be more communicative with students in English in class. Further studies were needed in relation to the CBI courses for students of different age levels.

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Contents Analysis of First Aid in Elementary, Middle and High School Textbooks (응급처치에 대한 초.중.고등학교 교과서 내용 분석)

  • Kim, Shin-Jeong;Lee, Jung-Eun;Kang, Kyung-Ah;Song, Mi-Kyung;Chang, Eun-Young;Kim, Sung-Hee
    • Child Health Nursing Research
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    • v.14 no.2
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    • pp.163-175
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    • 2008
  • Purpose: The purpose of this study was to provide basic data to develop first aid education programs for elementary, middle and high school students. Method: Forty nine textbooks adopted by first to six grade elementary schools and first to third grade of both middle and high schools were analyzed for content on first aid. Results: 1) First aid content was covered only in courses on and 2) Content on first aid was included in the categories, "General of first aid", "Rescue & moving", "Fire & Burns", "Cardiopulmonary resuscitation", "Musculoskeletal injuries", "Thermal injuries", "Wounds", "Removing foreign bodies", "Disaster & Accidents" and "Others". Categories that were not included were "Poisoning" and "Bites". The content did not follow a progression related to the level of intellectual and physical development of the students. Conclusion: These results suggest that the content on first aid should be revised to be practical and rational for the benefits of student safety.

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An International Comparative Study of 'Experiment' Presentation of Science Textbooks of Korea, Japan, and China (한국, 일본, 중국 과학교과서의 '실험' 내용 비교 분석 연구)

  • Byeon, Hyo-Jong;Baik, Mi-Hwa
    • Journal of Fisheries and Marine Sciences Education
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    • v.20 no.3
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    • pp.473-486
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    • 2008
  • This study was to compare 'experiment' presentation types of middle school science textbooks of Korea, Japan, and China and to draw some implication for the improvement of Korean science textbooks. It analyzed the experiments from the unit 'Light' of science textbooks which is common content in all three different science textbooks. The researchers analyzed the experiments with two questions. First, how is the experiment organized? Second, what role does each element of the experiment is playing in the experiment setting? The findings of international comparative analysis of experiment presentation types of middle school science textbooks of three countries were as follows. First, the Korean and Chinese textbooks tended to present the experiments without enough information for the experimental process and method, whereas the Japanese textbook tended to present the experiment with detailed information for the experimental method and process. Second, whereas the Korean and Chinese textbooks tended to have a loosened relationship among pre-experimental content, experiment, after-experiment content, the Japanese textbook tended to have an organic relationship among pre-experimental content, experiment, after-experiment content.

Children's Education Application Design Using AR Technology (AR기술을 활용한 어린이 교육 어플리케이션 디자인)

  • Chung, HaeKyung;Ko, JangHyok
    • Journal of the Semiconductor & Display Technology
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    • v.20 no.4
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    • pp.23-28
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    • 2021
  • Augmented reality is a technique for combining virtual images into real life by showing information of virtual 3D objects on top of a real-world environment (Azuma et al., 2001). This study is an augmented reality-based educational content delivery device that receives user input that selects either a preset object or a photographed object for augmented reality-based training; It includes a three-dimensional design generation unit that generates a stereoscopic model of the augmented reality environment from an object, a three-dimensional view of the scene, a disassembly process of the developing road from a three-dimensional model, and a content control unit provided by the user terminal by generating educational content including a three-dimensional model, a scene chart, a scene, a decomposition process, and a coupling process to build a coupling process from the scene to the three-dimensional model in an augmented reality environment. The next study provides a variety of educational content so that children can use AR technology as well as shapes to improve learning effectiveness. We also believe that studies are needed to quantitatively measure the efficacy of which educational content is more effective when utilizing AR technology.