• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.288-288
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• 2022

Rice is an important staple in the world. And drought is one of the important constraints that negatively affect yield loss and grain quality of rice. CRISPR/Cas9 is a new breeding strategy that can improve the characteristics of rice quickly and accurately. CRISPR/Cas9 is a novel approach that can reliably harvest rice yields in response to a rapidly changing climate. In addition, there is no externally inserted DNA left in genome-editing rice, and it is receiving attention as being able to take responsibility for future food because its characteristics are continuously improved. In the future, high levels of drought resistant in water-constrained environments will be required, which will reduce yield loss. OsSAP was genome-editing with CRISPR/Cas9 in rice. A different line number was assigned to each panicle, and the generation advanced by applying the ear-to-row method. Genome-editing rice has improved drought resistance in drought conditions. Also, in genome-editing rice, the target sequence was homozygous in the 0 generation, and the coefficient of variation of heading date, number of tiller, and 1,000-grain weight was very small in 2 generation. In the era of rapidly changing climate change, CRISPR/Cas9 presents a new breeding strategy that can rapidly and accurately improve agronomic traits of major food crops as well as rice. CRISPR/Cas9 is applied together with traditional breeding to develop into a new breeding strategy, it is suggested that food can be obtained stably in response to climate change.

• Journal of Korea Multimedia Society
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• v.20 no.8
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• pp.1456-1463
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• 2017

In recent times, IaaS (Infrastructure as a Services) have been rapidly evolving to allow developers to easily and efficiently access work in the server and network areas for development of a web of App based on cloud computing. In this study, we developed the collaborative image editing tool App based on Cloud-computing, by adopting AWS of representative company that develops IaaS. First, it is crucial to understand various situation conditions for representative infrastructure services: AWS, Azure and Google (GCP). This may have the effect of reducing manpower and development time, but as each company has different policy and technical support, we need a new study every time the environment changes of infrastructure services. We tried to develop a hybrid-App so that users with various devices can collaborate work each other by utilizing the infrastructure service AWS through the process of developing the image editing authoring tool based on the cloud computing. The future studies should continue about compatibility issues and support issues in order to minimize the problems of overseas infrastructure services, but we think that domestic cloud computing policies and developments should be urgently considered.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.107-114
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• 2017

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical bio-technologies. Earlier generations of knockout and gain-of-function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

• Transactions of the Korean Society of Mechanical Engineers
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• v.17 no.5
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• pp.1074-1083
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• 1993

NC simulation has been used to replace the test cutting of NC machining. Although it can reduce the NC part programming effort, it still has a problem. If any error is found during the simulation, then the part has to be reprogrammed and it is time consumming. This paper presents a method for verifying and editing the NC code after the post-processing without going back to the part programming stage. A data structure and an algorithm to verify and edit the NC code interactively with the aid of graphics is introduced. Z-map method is used for the shaded image display and cross-sectional view display of the macined parts. The method was implemented in a IBM/PC-386 with MS-Windows software, and the multi-window function of the of the MS-Windows is used for the simultaneous editing and verification.

• Journal of Internet Computing and Services
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• v.4 no.2
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• pp.31-37
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• 2003

Recently, motion capture has become one of the most promising technologies in animation. Realistic motion data can be captured by recording the movement of a real actor with an optical or magnetic motion capture system. This paper deals with motion editing by a geometric mean filter. Since the captured motion has some noises that cause a jerky motion, it needs a smoothing process to make it natural. A geometric mean filter is proposed to produce natural motions without jerky motions. Experimental results show that the geometric mean filter can effectively remove noises that cause a jerky motion and it can guarantee the most natural motions among various spatial filters. This method could be applied to the various fields such as real time animation, virtual reality applications, 3D applications, and etc.

• The Korean Journal of Applied Statistics
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• v.22 no.4
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• pp.697-707
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• 2009

In actual statistical surveys, the inconsistencies within the record are often occurred due to incorrect response. The users may be confused and statistical agencies may have a problem of reliability on statistical data in this case. It is needed to detect and correct the unconvinced record without any special reasons. However, it is not simple to determine which item should be corrected in every failed record. In this paper we briefly introduce Fellegi-Holt method, apply to a business survey, and then discuss the problems for this trial editing.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.360-372
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• 2020

Objective: Specific genomic sites can be recognized and permanently modified by genome editing. The discovery of endonucleases has advanced genome editing in pigs, attenuating xenograft rejection and cross-species disease transmission. However, off-target mutagenesis caused by these nucleases is a major barrier to putative clinical applications. Furthermore, off-target mutagenesis by genome editing has not yet been addressed in pigs. Methods: Here, we generated genetically inheritable α-1,3-galactosyltransferase (GGTA1) knockout Yucatan miniature pigs by combining transcription activator-like effector nuclease (TALEN) and nuclear transfer. For precise estimation of genomic mutations induced by TALEN in GGTA1 knockout pigs, we obtained the whole-genome sequence of the donor cells for use as an internal control genome. Results: In-depth whole-genome sequencing analysis demonstrated that TALEN-mediated GGTA1 knockout pigs had a comparable mutation rate to homologous recombination-treated pigs and wild-type strain controls. RNA sequencing analysis associated with genomic mutations revealed that TALEN-induced off-target mutations had no discernable effect on RNA transcript abundance. Conclusion: Therefore, TALEN appears to be a precise and safe tool for generating genomeedited pigs, and the TALEN-mediated GGTA1 knockout Yucatan miniature pigs produced in this study can serve as a safe and effective organ and tissue resource for clinical applications.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.154-160
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• 2015

The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding. Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology. Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency. Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.

• The Journal of Korean Association of Computer Education
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• v.14 no.4
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• pp.1-10
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• 2011

It is the basic direction of revised education course to bring up the autonomous and creative Koreans to lead the 21st century. In the music education of the middle school it could be said that music program using computer is a very effective medium for learners' sake of stimulating their audio-visual sense at the same time. In this study, I will look around the facility of the computer music program and tried to find the application method to connect the creative learning activity in music class. And I will also show the application method in the musical activity area. For this, I proposed the method for students' creative learning activity in the middle of using Finale 2010 for music notation, Window Moviemaker for image editing and Goldwave for sound editing.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.5
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• pp.843-850
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• 2006

The standardization on RDD and REL has been developed by MPEG. REL is a Right Expression Language and RDD is term dictionary which develop active application of REL. However, since REL documents could be oかy edited by user understanding MPEG-21's framework, much easier editing system is required. As well, REL Consumption System to process and analyze REL documents, the RDD interoperability system to interoperate REL and RDD are needed. In this paper, REL Editing System and REL Consumption System, RDD Web Services System were designed and implemented, REL Editing System to make REL document, REL Consumption System to process and analyze edited documents, RDD Web Services System to process rights inquiry base on the Web Services.