• 산업기술연구
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• 제24권B호
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• pp.139-142
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• 2004

To identify conserved structural or functional subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through sequence alignments of the CP1 domains, three conserved regions were found near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed that there exist three common structural subunits in all of the five editing RS structures. The new alignment suggests a translocation movement of the CP1 domain caused by the binding with tRNA. Based on the experimental and modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding.. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through all class Ia editing RSs.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.903-911
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• 2021

Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology. Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms. Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.

• 산업기술연구
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• 제42권1호
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• pp.29-36
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• 2022

The CRISPR system has revolutionized gene editing field. Cas9-mediated gene editing such as Indel induction or HDR enable targeted gene disruption or precise correction of mutation. Moreover, CRISPR-based new editing tools have been developed such as base editors. In this review, we focus on gene editing in human pluripotent stem cells, which is principal technique for gene correction therapy and disease modeling. Pluripotent stem cell-specific drug YM155 enabled selection of target gene-edited pluripotent stem cells. Also, we discussed base editing for treatment of congenital retina disease. Adenine base editor delivery as RNP form provide an approach for genetic disease treatment with safe and precise in vivo gene correction.

• 한국인쇄학회지
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• 제13권1호
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• pp.57-72
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• 1995

The function and technological merit of electronic image editing system using time-code addressing method are investigated. The method shortens the editing time and gives high accuracy. It is possible to digitize the editing system for high resolution and to vary and to systemize the VTR editing technique by this method.

• Molecules and Cells
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• 제46권1호
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• pp.4-9
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• 2023

The advent of the CRISPR-Cas genome editing platform has greatly enhanced the capabilities of researchers in many areas of biology. Its use has also been turned to the development of therapies for genetic diseases and to the enhancement of cell therapies. This review describes some recent advances in these areas.

• 한국게임학회 논문지
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• 제4권2호
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• pp.3-11
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• 2004

게임 엔진은 게임산업이 가지는 위험 요소를 최소화하기 위해서 사용되는 일반화된 방법으로 다양한 편집도구를 제공한다. 본 논문은 3차원 온라인액션게임 개발을 효과적으로 지원하기 위한 편집도구의 요구사항을 분석하고 이를 기반으로 설계와 구현한 결과를 기술한다. 편집도구의 요구사항을 분석하기 위해서 먼저 국내외 게임 엔진의 비교하였으며, 특별히 게임 엔진의 편집도구들의 종류와 특징에 대해서 살펴보았다. 이러한 요구사항 분석을 통해서 본격적인 게임 편집도구에 대한 설계 및 구현이 진행되었다. 이를 통해서 게임산업의 고부가가치화를 실현할 수 있을 것으로 예상된다.

• 한국발생생물학회지:발생과생식
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• 제21권3호
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• pp.343-349
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• 2017

tWith the development of the third-generation gene scissors, CRISPR-Cas9, concerns are being raised about ethical and social repercussions of the new gene-editing technology. In this situation, this article explores the legislation and interpretation of the positive laws in South Korea. The BioAct does not specify and regulate 'gene editing' itself. However, assuming that genetic editing is used in the process of research and treatment, we can look to the specific details of the regulations for research on humans as well as gene therapy research in order to see how genetic editing is regulated under the BioAct. BioAct differentiates the regulation between (born) humans and embryos etc. and the regulation differ entirely in the manner and scope. Moreover, due to the fact that gene therapy products are regarded as drugs, they fall under different regulations. The Korean Pharmacopoeia Act put stringent sanctions on clinical trials for gene therapy products and the official Notification "Approval and Examination Regulations for Biological Products, etc." by Food and Drug Safety Administration may be applied to gene editing for gene therapy purposes.

• 한국콘텐츠학회논문지
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• 제14권11호
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• pp.584-591
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• 2014

이 연구는 영화편집론에서 감정과 커뮤니케이션에 관한 논의를 고찰하였다. 커뮤니케이션 형식으로서의 편집을 통해, 관객은 이야기의 새로운 사실이나 그 사실의 세부로서 새로운 쇼트에 반응하며, 새로운 쇼트는 관객의 귀납적 능력을 이용한다. 쿨레쇼프는 유명한 모주킨 실험을 통해 편집이 개별 쇼트의 내용을 넘어서는 감정과 연상을 이끈다는 것을 보여주고자 했다. 180도 법칙, 시선과 동작의 일치, 30도 법칙, 사운드의 지속성, 빛과 색의 연속성 등의 기술을 활용하는 연속편집은 감정을 강화시킨다. 영화의 감정 효과를 극대화하는 대표적인 장치가 시점편집이다. 영화 내레이션의 목적에 봉사하는 시점편집은 감정 커뮤니케이션을 실행하고 설득하는 강력한 수단이다.

• BMB Reports
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• 제57권1호
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• pp.2-11
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• 2024

Advancements in gene and cell therapy have resulted in novel therapeutics for diseases previously considered incurable or challenging to treat. Among the various contributing technologies, genome editing stands out as one of the most crucial for the progress in gene and cell therapy. The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the subsequent evolution of genetic engineering technology have markedly expanded the field of target-specific gene editing. Originally studied in the immune systems of bacteria and archaea, the CRISPR system has demonstrated wide applicability to effective genome editing of various biological systems including human cells. The development of CRISPR-based base editing has enabled directional cytosine-to-thymine and adenine-to-guanine substitutions of select DNA bases at the target locus. Subsequent advances in prime editing further elevated the flexibility of the edit multiple consecutive bases to desired sequences. The recent CRISPR technologies also have been actively utilized for the development of in vivo and ex vivo gene and cell therapies. We anticipate that the medical applications of CRISPR will rapidly progress to provide unprecedented possibilities to develop novel therapeutics towards various diseases.

• 한국인터넷방송통신학회논문지
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• 제17권4호
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• pp.163-172
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• 2017

최근 방송영상분야에서 영상편집 방법으로 비선형편집(Non-Linear Editing; NLE)을 주로 사용하고 있다. 기존의 선형편집에 비해 비선형편집은 컷(Cut)의 삽입과 삭제가 용이하고, 영상편집 시 원하는 위치의 영상에 바로 접근할 수 있다. 또한, 타이틀과 효과, 장면전환 효과를 적용할 수 있고 출력 전에 미리보기를 통해 적용한 타이틀과 효과를 확인하고 수정하는 것이 용이한 장점이 있다. 그러나, NLE편집을 처음 접하는 학생들이 그것을 배우는 것은 쉽지 않다. 본 논문에서는 비선형편집을 처음 접하는 학생들이 쉽게 배울 수 있는 기존의 상호동료교수법(Reciprocal Peer Tutoring; RPT)를 보완한 새로운 RPT 학습모형을 제시한다. 제안하는 교수학습모형을 적용한 실험집단과 적용하지 않는 비교집단으로 나누어 실험을 실시한다. 두 집단의 전체 평균, 성적 하위 집단의 학업 성취도, 표준편차, T검정과 함께 설문조사를 통한 만족도를 실시한다. 제안하는 학습모형을 적용한 실험집단이 통제집단에 비해 지표와 만족도에서 우월함을 보인다.