• Biomolecules & Therapeutics
• /
• v.1 no.2
• /
• pp.188-195
• /
• 1993

In order to understand the machanism of action and regulation of ${\beta}$-adrenergic receptor in terms of molecular level, the purification of receptor protein has a fundamental importance. Moreover, species differences among avian, amphibian and mammalian ${\beta}$-adrenergic receptors make it more important to purify mammalian ${\beta}$-adrenergic receptor. Because ${\beta}$-adrenergic receptor is an integral membrane protein, it must be solubilized from the membrane for the purification. The purpose of the present study was to solubilize and characterize the mammalian $\beta$-adrenergic receptor from guinea pig lung in quantities by more efficient and practical method eventually to purify receptor. Guinea pig lung membrane preparation was solubilized by sequential treatment of buffers containing low and high concentration of digitonin which are 0.2 and 1.2% respectively. About 50% of the total receptor pool was released by this double extraction procedure. The $\beta$-adrenoceptors in the digitonin extract were identified using the ${\beta}$-adrenergic antagonist, (-)-[$^3H$]-dihydroalprenolol ([$^3H$]DHA). The solubilized receptor retained all of the essential characteristics of membrane-bound receptor, namely saturability; stereoselectivity; high affinity to ${\beta}$-adrenergic drugs. For the measurement of soluble receptor activity, Sephadex G-50 chromatography method has been widely used. Inspite of its accuracy and wide acceptance, this technique employed troublesome column work which required long time to assay the activity of receptor. We employed another methods to measure receptor activity. When using 0.5% polyethylenimine pretreated GF/B glass fiber filter, filtration technique could be used to measure soluble receptor activity. This technique enabled us to reduce the total amount of time to assay by a factor of 4 as well as to detect soluble receptor. In the present study, we could establish more efficient and practical solubilization method of mammalian $\beta$-adrenergic receptor. The rapidity and high yield of this solubilization scheme, together with the favorable recovery of the receptor activity, are significant steps toward the ultimate purification of the mammalian $\beta$-adrenergic receptor. The result of this study together with more convenient purification method could provide large amount of purified receptor with ease for various research purposes.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.21 no.1
• /
• pp.85-93
• /
• 2013

The amount of salted food waste and non-salted food waste from household consisted of 4 family members was surveyed. And the feeding rate and growth rate of Eisenia fetida population upon the different food wastes such as vegetables, grains, fruits and salted food waste were investigated. Total amount of food waste from a household was 3,200g/week. i.e. 0.114kg/day/capita and non-salted food waste was 53.9% of food waste. Salt contents of vegetables, grains, fruits were 0.60, 0.36 and 0.33%, respectively, if they were assumed to have 70% water content. Salt content of salted food waste was 0.78%. Upon the non-salted vegetables and fruits, feeding rate of earthworm was high but growth rate was low. Upon the non-salted grains, feeding rate of earthworm was low but growth rate was high. Upon the food with 70% water content, earthworm could not feed and grow when salt content of food was over 0.6%, and earthworm could feed normally but showed low growth rate when salt content of food was 0.3%. These results indicated that earthworm could stabilize the non-salted food wastes with ease but could hardly stabilize the salted food wastes.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
• /
• v.6 no.2
• /
• pp.169-178
• /
• 2016

The number of people with disabilities is increasing recently in Korea, in particular the proportion of children with disabilities is approximately above 5%. While there are many studies focusing on satisfaction of payment policy due to increased concern about children with disabilities, the studies on comparison and the effect factor of user satisfaction depending on the cash and voucher are not sufficient. Based on the need to recognize and research this issue, the ultimate goal of this study is to lead to implications for the payment policy by investigating user satisfaction depending on the type of payment of policy on children with disabilities. The result that analyzes the effect of general characteristics on user satisfaction shows that in case of in-cash, children and the degree of disability affect user satisfaction. In the case of voucher, the gender of parents, the degree of disability and the average income affect it. The implication of this study suggests practical implication and policy implication. Implications of the study is that provide a customized service considering general characteristics. Second, It needs to extend the range of amount of payment of in-cash. Third, extend and pay the amount of in-cash, and the service of voucher. Fourth, provide low-income group(including public aid recipients and near poverty group) with voucher to ease financial burden.

• Journal of Aerospace System Engineering
• /
• v.17 no.6
• /
• pp.1-8
• /
• 2023

Research, such as developing alternative energy in the transportation field, including aviation, is being actively conducted to solve the issue of current climate change. Interest in ammonia fuel as a carbon free energy (CFE) source is increasing due to the ease of liquefaction and transportation and similarity in energy density to that of methanol. However, explosiveness and toxicity of ammonia make it difficult to handle. Therefore, in this study, stable ammonia production was attempted using relatively easy-to-handle urea water solution (UWS). High temperature steam was used to promote the hydrolysis of ammonia. In order to determine the causes for ammonia production below the theoretical equivalent ratio, it was suggested that there were not enough collisions to promote the hydrolysis based on the kinetic theory of gases. The hydrolysis of unreacted isocyanic acid (HNCO) was tested according to the change in water supply. As a result, an increased amount of ammonia produced was confirmed. The increased amount of ammonia produced in a certain section was dependent on the steam temperature and the flow rate of water supplied.

• KDI Journal of Economic Policy
• /
• v.14 no.1
• /
• pp.167-189
• /
• 1992

The most difficult problem one faces in estimating North Korea's GNP is the lack of basic national income data. In addition, there is no appropriate foreign exchange rate available to convert North Korea's GNP to dollar values. The physical indicators method is particularly useful in estimating North Korean GNP because it requires only a modest amount of data and obtains dollar GNP directly by applying a relationship between physical indicators and GNP, which is estimated from reference countries, to physical indicators of North Korea. The estimated result of North Korean GNP in 1990 is 27.1 billion dollars and per capita GNP 1,268 dollars. The trade participation ratio (Trade/GNP) implied by the GNP estimate was plausible and so was the ratio of fiscal expenditure to GNP. This paper examined the physical indicators method's logic, the quality of the North Korean data that was used in the estimation, and the plausibility of estimation result. Relatively simple data requirement, comparative ease of computation and plausible estimation results suggest that use of physical indicators method could enhance the reliability of North Korean GNP estimate.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.36 no.5
• /
• pp.380-385
• /
• 2010

Introduction: The iliac crest has been the accepted place to obtain bone for reconstruction in oral and maxillofacial surgery. The iliac crest has many advantages because of its accessibility, large amount of cancellous bone, relative ease of bone harvest, possibility of two team approach and ability to close the wound primarily. This study evaluated retrospectively the morbidity of bone harvesting from the anterior iliac crest to provide a logical guide for recognizing the complications and morbidities of an iliac crest bone graft. Materials and Methods: Fifty healthy patients (mean age of 35.5 years; range 7 to 59) underwent iliac crest bone harvesting for a maxillofacial reconstruction from January 2007 to September 2009 at the Department of Oral and Maxillofacial Surgery in Kyungpook National University Hospital. Age, sex, size and kind of grafted bone, duration of pain on donor site, duration of gait disturbance, sensory deficit, scar, contour defect were measured in each patients by retrospective research. Results: The mean duration of pain is 6.7 days, and mean duration of gait disturbance is 7.2 days. Most patients were free from gait disturbances and pain within 2 weeks and there was no correlation between the size of the harvesting block bone and the duration of gait disturbance or pain. However, this study showed that the duration of pain is associated with gait disturbance. In addition, most patients had no complaints regarding their surgical scar and contour defect, and only one patient had permanent impairment of the sensory function. Moreover, an iliac bone graft did not extend the length of hospitalization. Conclusion: This study suggests that split thickness bone harvesting from the inner table of the anterior iliac crest is a well accepted procedure with relatively low morbidity.

• Journal of Fashion Business
• /
• v.11 no.5
• /
• pp.35-50
• /
• 2007

The purpose of this study was to investigate the result of a market research on men's jacket in their age of 25 to 35, who wear fitted jackets most, and to examine the comparative analysis of pattern and the wearing evaluation between classic jacket and fitted jacket. The results are as follows; Firstly, according to the results of a market research, 32.0% of the target owned fitted jackets, and 28.7% expressed their desire to buy only fitted jackets. This means their concern and preference for fitted jacket with which they could show up young, fresh and slim look are as high as those for classic jacket. Secondly, classic jacket showed the higher value than fitted jacket in such items as chest girth, waist girth, hip girth, sleeve's hem girth, jacket length, shoulder length, side seam length, and across shoulder point length. On the other hand, fitted jacket showed the higher value than classic jacket in back armhole length, front length, collar point length and sleeve's vent length. Thirdly, classic jacket was larger than fitted jacket in 1/2 chest girth(1.0cm), 1/2 waist girth(2.0cm), 1/2 hip girth(1.0cm), back width(0.5cm), front width(0.5cm) and side width(1.0cm). However, fitted jacket was higher than classic jacket in the armhole depth(0.5). Fourthly, the results of appearance evaluation revealed that there was a significant difference in 6 items. In other words, classic jacket was evaluated more appropriate than fitted jacket in such items as back width, back length, back neck depth, chest girth, waist girth and hip girth. And the fitted jacket had a higher value than the classic jacket in shoulder angle item. Fifthly, In the functional evaluation, fitted jacket was evaluated more uncomfortable than classic jacket. And this means that fitted jacket doesn't have a proper ease amount in each part and it has a high armhole depth compared to classic jacket. Therefore, for more comfortable patternmaking of fitted jackets, it would be required a pattern design which reflect men's own shoulder lines and women designers' flexibility that will satisfy both appearance and function.

• Journal of Korean Society of Environmental Engineers
• /
• v.31 no.5
• /
• pp.341-345
• /
• 2009

Continuous dry anaerobic digestion of organic solid wastes (30% TS, Total Solids) comprised of food waste and paper was performed under mesophilic condition. During the operation, hydraulic retention time (HRT) was decreased as follows: 150 d, 100 d, 60 d, and 40 d, which corresponded to the solid loading rate of 2.0, 3.0, 5.0, and 7.5 kg TS/$m^3$/d, respectively. Volumetric biogas production rate ($m^3$/$m^3$/d) increased as HRT decreased, and the highest biogas production rate of 3.49${\pm}$0.31 $m^3$/$m^3$/d was achieved at 40 d of HRT. At this HRT, high volatile solids (VS) reduction of 76% was maintained, and methane production yield of 0.25 $m^3$/kg $TS_{added}$ was achieved, indicating 67.4% conversion of organic solid waste to bioenergy. The highest biogas production yield of 0.52 $m^3$/kg $TS_{added}$ was achieved at 100 d of HRT, but it did not change much with respect to HRT. For the ease feed pumping, some amount of digester sludge was recycled and mixed with fresh feed to decrease the solid content. Recirculation volume of 5Q was found to be the optimal in this experimental condition. Specific methanogenic activity (SMA) of microorganisms at mesophilic-dry condition was 2.66, 1.94, and 1.20 mL $CH_4$/g VS/d using acetate, butyrate, and propionate as a substrate, respectively.

• Journal of Korean Society of Environmental Engineers
• /
• v.36 no.9
• /
• pp.641-647
• /
• 2014

In this study, sludge waste which has a difficulty in treating it was used to manufacture a fiber type of biosorbent. To solve the problems such as the release of organic pollutants and the difficulty in separating solid from treated water, entrapment method using Ca-alginate was used to immobilize sludge waste. Considering ease of manufacture as well as improvement of adsorptive ability, the biosorbent was manufactured in the form of fiber type. Optimum immobilization condition for minimizing the amount of alginate used and maximizing the performance of biosorbent was determined to be 10 g/L alginate concentration, 40 g/L sludge concentration, and 0.3-0.4 mm fiber diameter. The maximum Cd(II) uptake of the biosorbent was 60.73 mg/g. Pseudo-second-order kinetic model and Langmuir isotherm model adequately described the dynamic and equilibrium behaviors of Cd(II) biosorption onto the biosorbent, respectively. In conclusion, sludge waste generated from wastewater treatment process is a cheap raw material for the manufacture of biosorbent which can be used to remove toxic heavy metals from industrial wastewaters efficiently.

• Journal of Technology Innovation
• /
• v.25 no.4
• /
• pp.189-209
• /
• 2017

Financial institutions around the world, including financially advanced nations, widely operate a credit information sharing system to ease off information asymmetry between financial institutions and financial consumers. This study analyzed the credit problem data that is actually being shared among financial institutions in Korea, and classified credit problem data into three categories; Frequency, Period, Amount. In survival analysis, this study analyzed how different types of credit problem influence on survival period of companies. Next, in comparative analysis, this study verified a difference between start-up companies and existing companies on classified conditions of the credit problems. After conducting a survival and comparative analysis of the credit information of 449,579 companies of 8 years' actual information sharing in Korea, it showed that the number of the frequency of accidents showed a positive(+) correlation with the survival period. This provides contrary evidence to the financial institutions' risk policies that the number of the frequency of accidents is a negative factor. Furthermore, since the start-up companies that are under 7 years old show more positive aspect in the survival period than existing companies, it draws a policy implication that the credit information sharing system need to be improved by taking account of characteristics of the start-up companies.