• International Journal of Naval Architecture and Ocean Engineering
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• 제7권6호
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• pp.964-978
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• 2015

Numerous experiments with a scaled pilot facility were carried out to compare the relative bulk transfer performance of three special devices for applications to drilling systems. The pipe diameter for bulk transportation was 3 in., which corresponds to around half of the actual system dimensions. Two different pressures, 3 and 4 bar, were considered to check the relative performance under different pressure conditions at a bulk storage tank. And to make a practical estimation method of the bulk transfer rate at the early design stages of the bulk handling system, a series of experiments were conducted for real scaled bulk handing systems of two drilling vessels. The pressure drops at each pipe element as well as the bulk transfer rates were measured under different operating conditions. Using the measured results, the friction factor for each pipe element was calculated and a procedure for transfer rate estimation was developed. Compared to the measured transfer rate results for other drilling vessels, the estimated transfer rates were within a maximum 15% error bound.

• 한국가축번식학회지
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• 제13권2호
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• pp.98-104
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• 1989

Successful techniques of in vitro fertilization(IVF) are valuable for studying the process of fertilization and for developing economical procedures for gene and nuclear transfer in farm animals. To date, bovine IVF system has been developed with oocytes in vitro or vitro, but the resulting zygotes exhibit limited embryonic development after in vitro culture. Even though in vitro matured oocytes achieved high fertilization and cleavage rates, these embryos appear extremly low rate of pregnancies when transferred to synchronized recipients. Development of early bovine embryos in vitro is generally arrested at the 8-to 16-cell stage. However, recent use of somatic cells such as trophoblastic vesicle, granulosa and oviduct epithelial cell for co-culture with early bovine embryos has proven effective for development of embryos, matured and fertilized in vitro, past the in vitro cell blocks. These factors clearly indicate the value of the co-culture system in promoting development of bovine oocytes matured and fertilized in vitro to morula or blastocyst stage in vitro. In addition, co-culture system may beome a tool for evaluation of viability of ova that have been manipulated by procedures such as splitting, microinjection and nuclear transfer.

• 대한기계학회논문집B
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• 제22권8호
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• pp.1183-1194
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• 1998

This paper describes a theoretical model developed for analyzing the heat transfer of automotive cooling systems. From the model, heat transfer rate of automotive cooling systems can be predicted, providing useful information at the early stages of the design and development. The aim of the study is to develop a simulation program for automotive cooling system analysis and a performance analysis program for analyzing heat exchanger. Heat release rate from combustion gas to coolant through cylinder wall in engine cylinder was analyzed by using a two zone combustion model. This paper studied how cooling condition would affect engine heat release rate and measured temperature distribution of coolant in water jacket.

• 대한기계학회논문집A
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• 제21권10호
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• pp.1648-1655
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• 1997

The intake noise, a major source of vehicle noises, has rapidly become a noticeable, and has been studied to reduce the level. Traditionally, the intake system has been developed through a experiment, namely, the trial and error process. This approach requires very high cost and long time consuming to develop the system. Recently, FEM and BEM are becoming useful in analysis of the intake system, and te results of analysis are very valid. But because these techniques also require high cost and long analysis time, these are generally not practical tool at the early stage of the development of an intake system. In this study, the software was developed to predict and analyze the acoustic characteristics of an intake system. It was based on the Transfer Matrix Method and operated to analyze a simplified intake system in a personal computer. It can be used early in the design stage of development of the intake system. This study presented an improvement to reduce the level of an intake noise. It was to select the optimum position of a resonator and verified by NIT/SYSNOISE, FE analysis commercial software, and testing a prototype.

• 대한기계학회논문집A
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• 제21권10호
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• pp.1656-1665
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• 1997

The intake noise, a major source of vehicle noises, has rapidly become a noticeable, and has been studied to reduce the level. Traditionally, the intake system has been developed through a experiment, namely, the trial and error process. This approach requires very high cost and long time consuming to develop the systm. Recently, FEM and BEM are becoming useful in analysis of the intake system, and the results of analysis are very valid. But because this techniques also require high cost and long analysis time, this technique is generally not practical tool at the early stage of the development. In this study, the software was developed to predict and analyze the acoustic characteristics of the intake system. It was based on the Transfer Matrix Method and operated to analyze a simplified intake system in a personal computer. It can be used early in the design stage of development of the intake system. This study presented a improvement to reduce the level of the intake noise, which modified the specification of the intake system. And the improvement were verified by NIT/SYSNOISE, FE analysis commercial software, and testing a prototype.

• International Journal of Air-Conditioning and Refrigeration
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• 제13권1호
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• pp.51-60
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• 2005

This paper reviews the studies on the pressure drop and the heat transfer in microchannels. Although a lot of studies about the single-phase flow have been done until now, conflicting results are occasionally reported about flow transition from laminar flow to turbulent flow, friction factor, and Nusselt number. Some studies reported the early flow transition due to relatively greater wall effect like surface roughness, but the other studies showed that the flow transition occurred at the Reynolds number of about 2300 and the early flow transition might be due to less accurate measurement of the channel geometry. Also, there have been arguments whether the conventional relation based upon continuum theory can be applied to the fluid flow and the heat transfer in microchannels without modification or not. The studies about the two-phase flow in microchannels have been mostly about investigating the flow pattern and the pressure drop in rectangular channels using two-component, two-phase flow like air/water mixture. Some studies proposed correlations to predict two-phase flow pressure drop in microchannels. They were mostly based on Lockhart-Martinelli model with modification on C-coefficient, which was dependent on channel geometry, Reynolds number, surface tension, and so on. Others investigated the characteristics of flow boiling heat transfer in microchannels with respect to test parameters such as mass flux, heat flux, system pressure, and so on. The existing studies have not been fully satisfactory in providing consistent results about the pressure drop and the heat transfer in microchannels. Therefore, more in-depth studies should be done for understanding the fundamentals of the transport phenomena in the microchannels and giving the basic guidelines to design the micro devices.

• 기록학연구
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• 제63호
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• pp.115-144
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• 2020

이 논문은 일본 가나가와현 아카이브의 중간보존시설 운영과 현용기록의 조기이관 제도, 평가·선별 제도 연구를 통해 한국 기록관리 제도 개혁을 위한 참고 사례를 제공한다. 가나가와현 아카이브가 운영하는 중간보존 시설은 일본의 기록관리 개혁과 공문서관리법 제정 과정에서 주요 참조 모델로 검토된 바 있으며, 오키나와현 아카이브 등에서 '선진 사례'로 도입, 운영되고 있다. 또한, 가나가와현 아카이브는 '영구' 보존기간을 폐지하고, 10년과 30년 보존의 기록을 생산 종결된 때로부터 5년 경과 시점에 아카이브의 중간보존시설로 조기 이관한다. 기본적으로 보존기간 만료시 모든 현용기록을 재평가하는 제도이며, 1년 보존문서를 제외한 3년과 5년의 단기 보존연한 기록과 10년 이상 장기 보존연한 기록의 평가·선별을 아카이브의 핵심 업무로 설정하고 있다.

• Nuclear Engineering and Technology
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• 제51권1호
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• pp.60-72
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• 2019

The air-cooled passive decay heat removal system (APDHR) was proposed to provide the ultimate heat sink for non-LOCA accidents. The APDHR is a modified one of Passive Auxiliary Feed-water system (PAFS) installed in APR+. The PAFS has a heat exchanger in the Passive Condensate Cooling Tank (PCCT) and can remove decay heat for 8 h. After that, the heat transfer rate through the PAFS drastically decreases because the heat transfer condition changes from water to air. The APDHR with a vertical heat exchanger in PCCT will be able to remove the decay heat by air if it has sufficient natural convection in PCCT. We conducted the thermal-hydraulic simulation by the MARS code to investigate the behavior of the APR + selected as a reference plant for the simulation. The simulation contains two phases based on water depletion: the early phase and the late phase. In the early phase, the volume of water in PCCT was determined to avoid the water depletion in three days after shutdown. In the late phase, when the number of the HXs is greater than 4089 per PCCT, the MARS simulation confirmed the long-term cooling by air is possible under extended Station Blackout (SBO).

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.75-75
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• 2002

The present study was conducted for the production of transgenic cloned cows by somatic cell nuclear transfer (SCNT) that secrete human prourokinase into milk. To establish an efficient production system for bovine transgenic SCNT embryos, the offset was examined of various conditions of donor cells including cell type, size, and passage number on the developmental competence of transgenic SCNT embryos. An expression plasmid far human prourokinase (pbeta-ProU) was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and a human prourokinase target gene into a pcDNA3 plasmid. Three types of bovine somatic cells including two adult cells (cumulus cells and ear fibroblasts) and fetal fibroblasts were prepared and transfected using a lipid-meidated method. In Experiment 1, developmental competence and rates of GFP expression in bovine transgenic SCNT embryos reconstructed with cumulus cells were significantly higher than those from fetal and ear fibroblasts. In Experiment 2, the effect of cellular senescence in early (2 to 4) and late (8 to 12) passages was investigated. No significant differences in the development of transgenic SCNT embryos were observed. In Experient 3, different sizes of GFP-expressing transfected cumulus cells [large (>30 ${\mu}{\textrm}{m}$) or small cell (<30 ${\mu}{\textrm}{m}$)] were used for SCNT. A significant improvement in embryo development and GFP expression was observed when small cumulus cells were used for SCNT. Taken together, these results demonstrate that (1) adult somatic cells could serve as donor cells in transgenic SCNT embryo production and cumulus cells with small size at early passage were the optimal cell type, and (2) transgenic SCNT embryos derived from adult somatic cells have embryonic development potential.

• 한국수정란이식학회지
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• 제11권3호
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• pp.201-210
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• 1996

This experiment was carried out to evaluate the effect of early mouse embryonic development in vitro by co-culture with bovine and porcine oviductal epithelial cells (BOEC and POEC). The 2-cell embryos were collected from the oviducts of the superovulated and mated cultured in D-PBS /15% FCS at 48 hours after hCG injection. The in vitro developmental rate of blastocyst formation in the embryos were examined under the fllowing treatments; 1) TCM 199 added 15% HCS, 2) Ham's F-10 added 15% HCS, 3) MediCult IVF medium, 4) TCM 199 added 15% HCS + BOEC, 5) TCM 199 added 15% HCS + POEC, 6) Ham's F40 added 15% HCS + BOEC, 7) Ham's F-10 added 15% HCS + POEC,8) MediCult IVF medium + BOEC, 9) MediCult IVF medium + POEC. For a comparative study of in vitro development for 96 hours after hCG injection, were cultured with oviductal epithelial cell and media only. The obtained results were 2-cell embryos developed to the blastocyst stage in TCM 199, Ham's F-10 and MediCult IVF medium at the rates of 84.4,83.2 and 81.6%. respectively. The higher developmental rates(91~97%) of blastocyst formation was appeared when the embryos were co-cultured with a monolayer of bovine or porcine oviductal epithelial cells in TCM 199 or Ham's F-10 and MediCult IVF media. No significant difference in developmental rates was shown between bovine and porcine oviductal epithelial cells but significant difference in co-culture system in comparison between media only system and co-cultures. In conclusions, oviductal epithelial cells, BOEC and POEC, when co-culture with mouse early embryos improved the rates of development, blastocyst and hatching. Therefore, it is suggested that co-culture system using oviductal epithelial cells improve early embryonic developtnent in mouse.