• Molecules and Cells
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• v.25 no.2
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• pp.272-278
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• 2008

The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.169-175
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• 2022

This study aimed to examine the changes in dry matter yield and growth characteristics of alfalfa (Medicago sativa L.) in response to variations in sowing dates during the autumn season of 2021-22 in a dry paddy field of Chilbo-myeon, Jeongeup-si, Jeollabuk-do. Treatments comprised four sowing dates at 10-day intervals, i.e., October 8, October 18, October 28, and November 8, 2021. The winter survival rate of alfalfa showed a significant difference between different treatments but was at a satisfactory level for all (p<0.05). The winter survival rate for the fourth sowing date, a month later than the first sowing date, was approximately 11.7% lower than that for the first sowing date. The plant height ranged between 82.3-93.1 cm and 60.5-63.7 cm at the first and second harvest, respectively, smaller at the second harvest than at the first harvest. The total dry matter yield of alfalfa was the highest at 13,316 kg/ha for the first sowing date, and the later the sowing date, the lower the dry matter yield. The protein content of alfalfa ranged between 13.6-17.3% in the first harvest, lower than the standard alfalfa protein content of 20% or more. In relative feed value, the first sowing (Oct. 8) was the most significantly higher in the first harvest (p<0.05). These results suggest that the early and mid-October sowing dates are optimum for sowing alfalfa during autumn and result in improved plant growth, dry matter yield, protein content, and winter survival compared to those at later sowing dates. Therefore, dry paddy fields can be safely employed for alfalfa cultivation with sowing dates in early and mid-October during autumn.

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.109-115
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• 2000

Effects of the heavy-ion ($^{14}$ N or $^{20}$ Ne) beam irradiation on the response of anthers, growth of calli, germination of seeds, and the early growth after the germination of tobacco (Nicotiana tabacum L. cv. BY-4) were investigated. Anthers precultured for 10 days before the irradiation became brown without callus or shoot induction over 20 Gy of $^{14}$ N and $^{20}$ Ne ion beam irradiation. Relative growth rate of the cultured calli was reduced by the irradiation and became brown significantly 2 weeks after the $^{14}$ N and $^{20}$ Ne ion beam irradiation over 50 Gy. The increased intensity of the heavy-ion ($^{14}$ N, $^{20}$ Ne) beam irradiation resulted in the delay of seed germination and the inhibition of the early growth both in water-treated and non-treated seeds before the irradiation. In addition, the heavy-ion beam irradiation to the imbibed seeds inhibited seed germination more than that to the non-imbibed seeds. The screening approach of non-imbibed seeds with heavy-ion beam irradiation using in vitro culture system was more useful than the filter-paper germination method in investigating the characteristics of heavy-ion beam-irradiated seed population and the screening of morphological variants at the early stage of the plant growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.738-746
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• 1995

Greenhouse studies were carried out to find the difference of growth, weed competition and herbicides response in barley(Hordeum vulgare L. emend Larnark) under foggy and non-foggy condition. Plant height, leaf stage, leaf width and shoot fresh weight of barley under foggy condition were greatly increased, while heading rate ripening rate and number of grains per panicle of barley were reduced. Weed emergence based on fresh weight was much greater under foggy than that under non-foggy condition. Plant height of barley under foggy condition was increased comparing with non-foggy condition and significantly reduced with increasing the duration of weed competition, while 1,000-grain weight of barley reduced by the early competition(0∼20 days). Among the herbicides treated, butachlor and thiobencarb inhibited growth of barley under foggy than non-foggy condition. Plant height of barley treated of herbicides under foggy condition was ever increased but 1,000-grain weight of barley was reduced. Weeding efficacy(75-90%) by shoot fresh weight of weeds under foggy condition at 25 days after application was lower 3 to 15% than that under non-foggy condition.

• Journal of Embryo Transfer
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• v.10 no.3
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• pp.229-236
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• 1995

The experiments reported here take advantage of the large number of in vitro matured and in vitro fertilized(IVM /IVF) bovine oocytes which can be produced, permitting the design of controlled experiments to establish a simple defined medium for the study of early embryo requirements. A total of 1,386 IVM /IVF oocytes were used to compare a simple defined medium(KSOM) with more complex culture conditions used successfully for culture of bovine embryos but do not permit study of specific requirements. All experiments were extensively replicated factorials. In Experiment 1, KSOM was superior to Menezo B$_2$ medium in producing morulae plus blastocysts from IVM /IVF oocytes(33 vs 20%, P<0.()5). The yield of morulae plus blastocysts with KSOM was 22% and with BRLC added was 30%. In Experiment 2, (a 2x2 factorial of KSOM with or without BRLC and 0, 1 ng /ml of platelet derived growth factor, PDGF) more morulae plus blastocysts (40%) were produced in KSOM-BRLC co-culture containing 1 ng /ml PDGF than in the control KSOM(12%). In Experiment 3, there was no dose response when 0, 1 and 5 ng /ml of PDGF were added. The results with simple defined KSOM medium are sufficiently promising to indicate that specific requirements of the embryo may be examined in future studies with KSOM as a base.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.1
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• pp.89-94
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• 1997

The present study was conducted to know the growth and yield of in response to the drought stress at the different soybean growth stage. Drought stress was given to the soybean plants on early vegetative growth at fourth-node stage(FNS), mid-growth at beginning pod stage(BPS) and late growth at beginning seed stage(BSS) for 30 days, which are high availability in soil water stress on climate condition of Korea. Dry weight was decreased severely by water stress at FNS, and BPS and BSS has no difference compared to control. Chlorophyll content of leaf severely decreased at the end of water stress of FNS and BPS, but was recovered at the harvest stage. Drought-stressed root distributed mainly near the soil surface and number and dry weight of root nodule were decreased severely by drought stress at BPS. Number of pod, seed weight and yield were decreased by drought stress and showed the highest yield loss at BPS.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.264-270
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• 2017

Brassinosteroid (BR), a plant steroid hormone, plays a critical role in the growth and developmental processes through its canonical signaling and crosstalk with various internal and external signaling pathways. Recent studies have revealed the essential interplay mechanisms between BR and ABA during seed germination and early seedling establishment. However, molecular mechanisms for this important signaling crosstalk are largely unknown. To understand the crosstalk between BR-mediated signaling pathways and ABA functions during early seedling development, we carried out a comparative genome-wide transcriptome analysis with an Agilent Arabidopsis $4{\times}44K$ oligo chip. We selected and compared the expression patterns of ABA response genes in ABA-insensitive bes1-D mutant with wild type seedlings on which ABA was exogenously applied. As a result, we identified 2,353 significant differentially expressed genes (DEGs) in ABA-treated bes1-D and wild type seedlings. GO enrichment analysis revealed that ABA signaling, response, and metabolism were critically down-regulated by BR-activated signaling pathways. In addition, the genome-wide transcriptome analysis data revealed that BR-regulated signaling pathways were tightly connected to diverse signal cues including abiotic/biotic stress, auxin, ROS etc. In this study, we newly identified the molecular mechanisms of BR-mediated repression of ABA signaling outputs. Also, our data suggest that interplay among diverse signaling pathways is critical for the adaptive response of the plant to various environmental factors.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.471-474
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• 2002

This experiment was conducted to investigate the response of rice growth and grain yield to night illumination. Three cultivars Heugjubyeo (early maturing), Hwaseongbyeo (medium maturing), and Ilpumbyeo(mid-late maturing) were tested under different light intensities ranging 1.0 to 20.0 lux (0.3 to 5.6 $1\mu\textrm{W}$ $cm^{-2}$). Awn length of all cultivars became longer as night illumination intensity increased; 4.09 cm under 10-20 lux compared to 1.93cm under 2 lux in Hwaseongbyeo. However culm and spike length ere not affected by night illumination. Night illumination delayed flowering by 3 days in Heugjinjubyeo, 5 days in Hwaseongbyeo, and 9 days in Ilpumbyeo at 10-20 lux compared to that at 2 lux. Grain yield was reduced by 10.0% in Heugjinjubyeo, 17.7% in Hwaseongbyeo, and 20.7% in Ilpumbyeo at 10-20 lux compared to 2 lux condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.3
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• pp.9-14
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• 1980

This experiment was carried out to examine the effect of water stress at anthesis on the grain maturation and to interpret the immediate and resulting growth response in barley. The dry weight of root, stem, internode, flag leaf and grain under water stress was remarkably more reduced than that under no-water stress, respectively. Water stressed plants had heavier grain dry weight during 7.14 days after water stress than that under no-water stress, but this early response was reversed significantly in the later period. The relative turgidity of organs except grain under water stress was severely lowered than that under no-water stress. The net photosynthsis by rewatering after water stress had been kept at the lower level than that under no-water stress ti1l the late maturation.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.723-731
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• 2008

Immunomodulatory feed additives might offer alternatives to antimicrobial growth promoters in pig production. This experiment was designed to determine the effects of dietary galacto-mannan-oligosaccharide (GMOS) and chitosan oligosaccharide (COS) supplementation on the immune response in early-weaned piglets. Forty 15-day-old piglets (Duroc$\times$Landrace$\times$Yorkshire) with an average live body weight of $5.6{\pm}0.51kg$ were weaned and randomly assigned to 4 treatment groups that were fed maize-soybean meal diets containing either basal, 110 mg/kg of lincomycin, 250 mg/kg of COS or 0.2% GMOS, respectively, over a 2-week period. Another six piglets of the same age were sacrificed on the same day at the beginning of the study for sampling, in order to obtain baseline values. Interleukin (IL)-1${\beta}$gene expression in peripheral blood monocytes, jejunal mucosa and lymph nodes, as well as serum levels of IL-1${\beta}$ IL-2 and IL-6, IgA, IgG, and IgM, were evaluated for 5 pigs from each group at 15 and 28 days of age. The results indicate that weaning stress resulted in decreases in serum antibody and cytokine levels. Dietary supplementation with GMOS or COS enhanced (p<0.05) IL-1${\beta}$gene expression in jejunal mucosa and lymph nodes, as well as serum levels of IL-1${\beta}$ IL-2, IL-6, IgA, IgG and IgM compared to supplementation with lincomycin. These findings suggest that GMOS or COS may enhance the cell-mediated immune response in early-weaned piglets by modulating the production of cytokines and antibodies, which shows that GMOS or COS have different effects than the antibiotic on animal growth and health.