• Korean Journal of Veterinary Service
• /
• v.45 no.2
• /
• pp.117-124
• /
• 2022

In the event of an outbreak of a livestock epidemic, it has been considered that the existing burial-centered carcass disposal method should be improved ecofriendly for prevention of leachate and odors from burial basically in regard of pathogen inactivation. Therefore, the aim of this study is whether it was possible to treat the carcass of cattle and chickens using the chemical carcass treatment method. It was conducted to establish detailed treatment standards for the chemical treatment method of cattle and chicken carcasses based on the results of the proof of the absence of infectious diseases in cattle chickens. After inoculating cattle carcass with 10 pathogens (foot and mouth disease virus, bovine viral diarrhea virus, Mycobacterium bovis, Mycobacterium avium subsp. Paratuberculosis, Brucella abortus, Bacillus anthracis, Clostridium chauvoei, Clostridium perfringens, Escherichia coli, and Salmonella Typhimurium) and chicken carcasses with low pathogenic avian influenza virus, Clostridium perfringens type C, E. coli and Salmonella Typhimurium, these were treated at 90℃ for 5 hours in a potassium hydroxide liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens by PCR and culture. Based on these results, it was possible to prove that chemical treatment of cattle and chicken carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. The chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

• Biomolecules & Therapeutics
• /
• v.31 no.1
• /
• pp.97-107
• /
• 2023

Aristolochic acid (AA), extracted from Aristolochiaceae plants, plays an essential role in traditional herbal medicines and is used for different diseases. However, AA has been found to be nephrotoxic and is known to cause aristolochic acid nephropathy (AAN). AA-induced acute kidney injury (AKI) is a syndrome in AAN with a high morbidity that manifests mitochondrial damage as a key part of its pathological progression. Melatonin primarily serves as a mitochondria-targeted antioxidant. However, its mitochondrial protective role in AA-induced AKI is barely reported. In this study, mice were administrated 2.5 mg/kg AA to induce AKI. Melatonin reduced the increase in Upro and Scr and attenuated the necrosis and atrophy of renal proximal tubules in mice exposed to AA. Melatonin suppressed ROS generation, MDA levels and iNOS expression and increased SOD activities in vivo and in vitro. Intriguingly, the in vivo study revealed that melatonin decreased mitochondrial fragmentation in renal proximal tubular cells and increased ATP levels in kidney tissues in response to AA. In vitro, melatonin restored the mitochondrial membrane potential (MMP) in NRK-52E and HK-2 cells and led to an elevation in ATP levels. Confocal immunofluorescence data showed that puncta containing Mito-tracker and GFP-LC3A/B were reduced, thereby impeding the mitophagy of tubular epithelial cells. Furthermore, melatonin decreased LC3A/B-II expression and increased p62 expression. The apoptosis of tubular epithelial cells induced by AA was decreased. Therefore, our findings revealed that melatonin could prevent AA-induced AKI by attenuating mitochondrial damage, which may provide a potential therapeutic method for renal AA toxicity.

• Journal of the Korean Applied Science and Technology
• /
• v.40 no.3
• /
• pp.476-483
• /
• 2023

This study aimed to analyze the effects of athletic career and competition participation frequency on exercise commitment of women university taekwondo athletes. Study subjects were 20 women university taekwondo athletes. Athletic career and competition participation frequency was assessed by 4-points scale and the higher points indicate the higher level of each variables. Exercise commitment was assessed by Exercise Commitment Scale. The assessment consists of a total of 8 questions, 4 of which are action immersion and 4 of cognitive immersion, and is evaluated using a 5-point Likert scale. The higher the score, the higher the level of exercise commitment. As the results, positive relationship showed both correlation and casual relationship analysis between competition participation frequency and exercise commitment. Negative casual relationship (-) showed between athletic career and exercise commitment. These results indicated that the increase of competition participation frequency affects the exercise commitment and the longer of athletic career indicates the decrease the level of exercise commitment. Thus, to improve the exercise commitment of women university taekwondo athletes, the competition participation frequency and athletic career should be considered.

• Fisheries and Aquatic Sciences
• /
• v.26 no.2
• /
• pp.105-116
• /
• 2023

Improved fish post-harvest technologies (IFPT) have been promoted as more efficient methods of fish processing, preservation, and value addition than the traditional methods prevalent in developing countries. The adoption rates, however, do not appear to be convincing. The purpose of this study was to determine the socio-demographic and psychological factors that influence intention of Kenyan farmed fish traders to use IFPT. The technology acceptance model (TAM) was used to properly explain the impact of TAM constructs such as perceived usefulness (PU), perceived ease of use (PEOU), and attitude (ATT), as well as socio-demographic factors such as gender, age, education level and fish trading experience on traders' intention to use the technologies. A cross-sectional survey was conducted to collect data using a semi-structured questionnaire from 146 traders in Busia, Siaya and Kakamega counties. At a significance level of p = 0.05, a linear regression model was used to examine the socio-demographic and psychological determinants of the traders' behavioral intention to use the improved technologies. The regression analysis revealed that PU (β = 0.443; p = 0.000), PEOU (β = 0.364; p = 0.000) and ATT (β = 0.615; p = 0.000) influence traders' intention to use IFPT, with ATT having the highest influence on intention. However, the traders' socio-demographic characteristics have no effect on their intention to use the technologies, as the coefficients for gender (β = 0.148; p = 0.096), age (β = 0.016; p = 0.882), level of education (β = -0.135; p = 0.141) and fish trading experience (β = 0.017; p = 0.869) are all insignificant. These findings show that the traders intend to use IFPT and will use them when it is in their best economic interests.

• Korean Journal of Animal Reproduction
• /
• v.9 no.2
• /
• pp.133-139
• /
• 1985

An immunoassay may be defined as an analytical procedure involving the competitive reaction between a limiting concentration of specific antibody and two populations of antigen, one of which is labelled or immobillized. The advent of immunoassay has revolutionised our knowledge of reproductive physiology and the practice of veterinary and clinical medicine. Radioimmunoassay (RIA) was the first of these methods to be developed, which meausred the analyte with good sensitivity, accuracy and precision (1,2). The essential components of RIA are:-(i) a limited concentration of antibodies, (ii) a reference preparation, and (iii) an antigen labelled with a radioisotope (usually tritium or iodine-125). Most procedures invelove isolating the antibody-bound fraction and measuring the amount of labelled antigen. Good facilities are available for scintilltion counting, data reduction nd statistical analysis. RIA is undergoing refinement through:-(i) the introduction of new techniques to separate the antibody-bound and free fractions which minimize the misclassification of labelled antigen into these compartments, and the amount of non-specfic binding. (3), (ii) the development of non-extration for the measurement of haptens (4), (iii) the determination of a, pp.rent free (i.e. non-protein bound) analytes (5), and (iv) the use of monoclonal antibodies(6). In 1968, Miles and Hales introduced in important new type of immunoassay which they termed immunora-diometric assay (IRMA) based on t도 use of isotopically labelled specific antibodies(7) in a move from limited to excess reagent systems. The concept of two-site IRMAs (with a capture antibody on a solid-phase, and a second labelled antibody to a different antigenic determinant of the analyte) has enabled the development of more sensitive and less-time consuming methods for the measurement of protein hormones ovar wide concentration of analyte (8). The increasing use of isotopic methos for diverse a, pp.ications has exposed several problems. For example, the radioactive half-life and radiolysis of the labelled reagent limits assay sensitivity and imposes a time limit on the usefulness of a kit. In addition, the potential health hazards associated with the use and disposal of radioactive cmpounds and the solvents and photofluors necessary for liquid scientillation counting are incompatable with the development of extra-laboratory tests. To date, the most practical alternative labels to radioisotopes, for the measurement of analytes in a concentration > 1 ng/ml, are erythrocytes, polystyrene particiles, gold sols, dyes and enzymes or cofactors with a visual or colorimetric end-point(9). Increased sensitivity to<1 pg/ml may be obtained with fluorescent and chemiluminescent labels, or enzymes with a fluorometric, chemiluminometric or bioluminometric end-point. The sensitivity of any immunoassay or immunometric assay depends on the affinity of the antibody-antigen reaction, the specific activity of the label, the precision with which the reagents are manipulated and the nonspecific background signal (10). The sensitivity of a limited reagent system for the measurement of haptens or proteins is mainly dependent upon the affinity of the antibodies and the smalleest amount of reagent that may be manipulated. Consequently, it is difficult in practice to improve on the sensitivity obtained with iodine-125 as the label. Conversely, with excess reagent systems for the measurement of proteins it is theoretically possible to increase assay sensitivity at least 1000 fold with alternative luminescent labels. To date, a 10-fold improvement has been achieved, and attempts are being made to reduce the influence of other variables on the specific signal from the immunoreaction.

• Food Science of Animal Resources
• /
• v.43 no.4
• /
• pp.639-658
• /
• 2023

Although goat meat has several health benefits than other red meats but comsumers reluctant it due to its unpleasant flavor. This study aimed to investigate the odorant of goat meat as well as compare the quality traits of meat regarding sex status. The loin meats [non-castrated male (NCM), castrated male (CM), and female (FM)] were collected and stored at 4℃ in a laboratory refrigerator and analyzed on the 1^st, 5^th, and 8^th consecutive days. The moisture content was the lowest and the protein content was highest in FM (p<0.05). Fat and ash content in NCM and FM were similar while lowest in CM. The CIE L^* was significantly higher in NCM, but there were no significant differences of CIE a^* and CIE b^* within groups at initial day. The color intensity increased on the 5^th storage day and decreased again after the 8^th storage day, except in NCM. NCM displayed the highest thiobarbituric acid reactive substances value (p<0.05), whereas CM displayed a higher pH value than other groups throughout the storage period. Indole levels were the highest in NCM (0.031 mg/kg); however, skatole levels were not significant differences across all treatments (p>0.05). No significant difference was observed in the fatty acid composition between NCM and CM (p>0.05), whereas a significant difference was observed in FM (p<0.05). Most of the water-soluble metabolites showed significant differences between sexes. Overall, sex status effects on quality properties of meat and castration can improve the overall sensory acceptance by reducing goaty flavor of Korean native black goat meat.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.448-448
• /
• 2021

최근 급격한 기후변화와 도시화 및 산업화로 인한 지류하천에서의 수량과 수질의 변동은 생물 다양성 감소와 수생태계 건강성 저하에 큰 영향을 미치고 있다. 효율적인 수생태 관리를 위해서는 지속적인 유량, 수질, 그리고 수생태 모니터링을 통한 데이터 축적과 더불어 면밀한 상관 분석을 통해 수생태계 건강성의 악화 원인을 규명해야 할 필요가 있다. 그러나 수많은 지류하천을 대상으로 한 지속적인 모니터링은 현실적으로 어려움이 있으며, 수생태계의 특성 상 단일 영향 인자만으로 수생태계의 건강성 변화와의 관계를 정확히 파악하는데 한계가 있다. 따라서 지류하천에서의 유량 및 수질의 시공간적인 변동성과 다양한 영향 인자를 고려하여 수생태계의 건강성을 효율적으로 예측할 수 있는 기술이 필요하다. 이에 본 연구에서는 경험적 데이터 기반의 머신러닝 모델 구축을 통해 미계측 하천에서의 수생태계 건강성 지수(BMI, TDI, FAI)의 등급(A to E)을 예측하고자 하였다. 머신러닝 모델은 학습 데이터셋의 양과 질에 따라 성능이 크게 달라질 수 있으며, 학습 데이터셋의 분포가 불균형적일 경우 과적합 또는 과소적합 문제가 발생할 수 있다. 이를 보완하고자 본 연구에서는 실제 측정망 데이터셋을 바탕으로 생성적 적대 신경망 GAN(Generative Adversarial Network) 알고리즘을 통해 머신러닝 모델 학습에 필요한 추가 데이터셋(유량, 수질, 기상, 수생태 등급)을 확보하였다. 머신러닝 모델의 성능은 5차 교차검증 과정을 통해 평가하였으며, GAN 데이터셋의 정확도는 실제 측정망 데이터셋의 정규분포와의 비교 분석을 통해 평가하였다. 최종적으로 SWAT(Soil and Water Assessment Tool) 모형을 통해 예측 된 미계측 하천에서의 데이터셋을 머신러닝 모델의 검증 자료로 사용하여 수생태계 건강성 등급 예측 정확도를 평가하였다. 본 연구에서의 GAN에 의해 강화된 머신러닝 모델은 수질 및 수생태 관리가 필요한 우심 지류하천 선정과 구조적/비구조적 최적관리기법에 따른 수생태계 건강성 개선 효과를 평가하는데 활용될 수 있을 것이다. 또한 이를 통해 예측된 미계측 하천에서의 수생태계 건강성 등급 자료는 수량-수질-수생태를 유기적으로 연계한 통합 물관리 정책을 수립하는데 기초자료로 활용될 수 있을 것이라 사료된다.

• 한국체육학회지인문사회과학편
• /
• v.57 no.3
• /
• pp.117-133
• /
• 2018

The purpose of this study was to explore integrated approaches to physical education in general education by examining methodology of physical education aiming for convergence education. This case study was conducted, using a qualitative approach during March, 2017 to November, 2017. Data were collected through non-participant observation, in-depth interviews, field-notes, students' journal, syllabus and lecture materials. The key findings are as follows: First, "Emotion Coaching through Movement" is a course of 'understanding of body' approaching integrated humanities science and natural science. Second, it is a convergence education, conducting 'text to daily practice' by approaching positive psychology and neurophysiology. Third, it is a physical education with 'integrated theory and practice' in higher education. These results indicate that students can understand their own body, observe their daily and fixed movement or reaction pattern, and enhance the ability of understanding others through a physical education in general education.

• International journal of advanced smart convergence
• /
• v.11 no.4
• /
• pp.227-239
• /
• 2022

Purpose: In our study, in order to find lactic acid bacteria (LAB) with multi-drug resistance to antibiotics, we isolated 140 strains from 15 types of kimchi commercially available in Korea and 20 types of Kimchi made at home from January to December in 2016, and investigated their H₂O₂ generating ability and multi-drug resistance to antibiotics. Methods: In order to observe the H₂O₂ generation ability of LAB, we performed the experiment with methods such as Rabe, Hillier, and Kang. To test the antibacterial susceptibility of LAB, we used the disc agar diffusion method using MRS agar (Difco, USA) according to the CLSI and WHO test methods. There are 18 types of antibiotic discs used. Results: Out of the total numbers of 140 strains, 6 strains of Ent. Faecium, 25 strains of L. plantarum, 1 strain of L. rhamnosus, 3 strains of L. sakei, 1 strain of L. acidophilus, 1 strains St. thermophilus, and 7 of unidentified strains generated H₂O₂. The antibiotic susceptibility of Ent. Faecium indicated SXT, OX, NA, and E; and the antibiotic susceptibility of L. plantarum indicated NA; and the antibiotic susceptibility of St. thermophilus indicated NA, CC, RA, CTT, CM, and P ; and the antibiotic susceptibility of L. rhamnosus indicated SXT, VA, NA and CTT; and the antibiotic susceptibility of 6 strains of L. sakei indicated SXT, OX, NOR, NA, CTT and CIP, all indicating antibiotic resistance. In the case of multi-drug resistance to antibiotics for 53 strains of L. antarum, 8-drug resistance was the most common with 25 strains, followed by 7-drug-resistant strains with 18 strains, 9-drug-resistant strains with 4 strains, 6-drug-resistant strains with 3 strains, 5-drug-resistant strains with 2 strains, and 17-drug-resistant strains with 1 strain. In the case of multi-drug resistance to antibiotics for Ent. Faecium 27 strains, 9-drug resistance was most commonly identified as 9 strains, 8-drug resistance was identified as 6 strains, 7- and 11 drug resistances were identified as 4 strains each, and 4- and 6-drug resistances were identified as 1 strain each. Conclusion: Ent. Faecium, L. plantarum, L. rhamnosus, L. sakei, and St. thermophilus, shown to have anantibacterial activity in previous studies on LAB and shown to have and H₂O₂ generating ability, antibiotic resistance and multi-drug resistance in this study, are expected to be able to play an excellent role for long-term inpatients to use as an alternative to antibiotics and to cope with emerging antibiotic resistance.

• The Journal of Bigdata
• /
• v.8 no.1
• /
• pp.49-59
• /
• 2023

There are various items in the safety and health standards of the manufacturing industry, but they can be divided into work-related diseases and musculoskeletal diseases according to the standards for sickness and accident victims. Musculoskeletal diseases occur frequently in manufacturing and can lead to a decrease in labor productivity and a weakening of competitiveness in manufacturing. In this paper, to detect the musculoskeletal harmful factors of manufacturing workers, we defined the musculoskeletal load work factor analysis, harmful load working postures, and key points matching, and constructed data for Artificial Intelligence(AI) learning. To check the effectiveness of the suggested dataset, AI algorithms such as YOLO, Lite-HRNet, and EfficientNet were used to train and verify. Our experimental results the human detection accuracy is 99%, the key points matching accuracy of the detected person is @AP0.5 88%, and the accuracy of working postures evaluation by integrating the inferred matching positions is LEGS 72.2%, NECT 85.7%, TRUNK 81.9%, UPPERARM 79.8%, and LOWERARM 92.7%, and considered the necessity for research that can prevent deep learning-based musculoskeletal diseases.