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The Effect of Alcohol Administration on Selenium Concentration and Cell Morphology of Heat and Liver of Rats Fed with the Different Levels of Selenium and Vitamin E (알코올의 섭취가 식이내 Selenium과 Vitamin E 수준을 달리한 흰쥐의 체내 Selenium 수준과 심장 및 간조직에 미치는 영향)

  • 김갑순;채기수;정승용
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.6
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    • pp.664-672
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    • 1993
  • The purpose of this study was to investigate the effect of alcohol administration on selenium concentration and cell morphology in tissurs of rats fed with the different levels of selenium (Se) and vitamin E. Seventy two male rats of Sprague-Dawley strain weighing about 58~62g were divided into 12 groups. The dietary Se levels were 0mg(L-), 0.4mg(C-) and 10mg(H-), and the dietary vitamin E levels were 0mg(-L) and 150mg(-C) per kg diet, respectively. Alcohol-adminstrated groups(--A) received the triple distilled potable water solution containing 10% of ethanol from the 3rd week of experimental periods. The obtained experimental results are summarized as follows. Se concentration in blood and urine made difference in accordance with Se level in diet and tended to be low in alcohol administrated groups. Se concentration in liver and kidney was also directly proportional to the dietary Se level, and it tended to be low in each alcohol group, but Se concentration in kidney tended to be increased by alcohol administration. Myocardium in rats showed lysosome increasing, fat droplet, mitochondrial swelling, and in particular, bad intracellular edema, in H-group fed with high Se and in L-group with low Se. It also showed such phenomena in the alcohol administrated group. In HC-group fed with excessive Se and normal vitamin E. there appeared no noticeable change in liver tissue. However, in the alcohol administrated HCA-group, there came out fat droplet. Especially, in the alcohol administrated LLA-group, not fed with sufficient Se and vitamin, E, there were found lysosome increasing and a number of fat droplet.

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Protonation and Energetical Investigations of Calix[4]-cyclen-benzo-crown-6 and Its Complexes with Zinc and Copper

  • Boonchoo, Thanaporn;Pulpoka, Buncha;Ruangpprnvisuti, Vithaya
    • Bulletin of the Korean Chemical Society
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    • v.25 no.6
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    • pp.819-822
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    • 2004
  • Protonation constants of calix[4]-cyclen-benzo-crown-6, L in 1X$10^{-2}$ M $Bu_4NCF_3SO_3$ in 40% $CH_2Cl_2/CH_3OH$ at $25^{\circ}C$ determined by potentiometric titration are log $K_1$ = 10.91, log $K_2$ = 10.30, log $K_3$ = 6.24 and log $K_4$ = 2.55. Stability constants for the receptor L complexes with Cu(II) and Zn(II) in 1X$10^{-2}$ M $Bu_4NCF_3SO_3$ in 40% $CH_2Cl_2/CH_3OH$ at $25^{\circ}C$ were determined by UV-VIS spectrometric titration. Stability constants of the CuL and ZnL complexes as log $\beta$ are 4.37 and 3.45, respectively. Stabilization energies for protonations of receptor L, derived from ab initio Hartree-Fock method with 6-31G basis set, are ${\Delta}E_1$ = -290.1, ${\Delta}E_2$ = -205.0, ${\Delta}E_3$ = -124.9 and ${\Delta}E_4$ = -26.9 kcal/mol and complexation energy of ZnL complex is -370.3 kcal/mol.

Amplification of Glutathione Production in E. coli Cells Using Recombinant DNA Techniques

  • Nam, Yong-Suk;Park, Young-In;Lee, Se-Yong
    • Journal of Microbiology and Biotechnology
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    • v.1 no.3
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    • pp.157-162
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    • 1991
  • Conditions for glutathione production in E. coli cells which possess pGH501 (2 gshI+gshII) were studied. In terms of ATP supply for the glutathione synthesis, two different systems have been constructed and compared. When the acetate kinase reaction of E. coli was used for ATP generation, 20 mM of L-cysteine was completely converted to glutathione by toluene-treated E. coli cells (100 mg/ml) harboring pGH501 within 2 h at $37^{\circ}C$. However, considering the economical aspects, the glycolytic pathway of yeast was chosen as a better system for ATP generation. The optimal concentrations of reactants for glutathione production were determined to be as follows; 80 mM L-glutamate, 20 mM L-cysteine, 20 mM glycine, 20 mM $MgCl_2$, 50 mM potassium phosphate buffer (pH 7.5), 400 mM glucose, polyoxyethylene stearylamine ($5\;\mul/ml$), toluene-treated E. coli HB101/pGH501 (100 mg/ml), and dried yeast cells (400 mg/ml). The conversion ratio of L-cysteine to glutathione was 80% (about 5 mg/ml) under optimal condition within 6 h at $37^{\circ}C$.

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1-out-of-L Electronic Voting System with Ballot-Cancellation (투표-취소가 가능한 1-out-of-L 전자투표 시스템)

  • Yang, Hyung-Kyu;An, Youn-Hwa
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.1C
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    • pp.86-93
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    • 2007
  • In this paper, we present an electronic voting system based on cryptographic techniques. Recently, some countries have used e-voting systems using an electronic voting device instead of a voting sheet. These e-voting systems are the early stage which is not online voting. Many cryptographers have studied on-line e-voting systems based on cryptographic techniques. The existing 1-out-of-L e-voting systems are based on E1Gamal cryptosystem. To reduce computational complexity, we use r-th residue encryption scheme and compare the computational complexity of our 1-out-of-L e-voting system with that of the 1-out-of-L e-voting system based on E1Gamal cryptosystem. Moreover, we extend the proposed 1-out-of-L e-voting system to ballot-cancellation property The existing e-voting systems had been overlooked the ballot-cancellation property. There is the reason that the ballot is cancelled according to an election law. For our e-voting system with ballot-cancellation property, we extend the homomorphic property based on r-th residue encryption. The extended homomorphic property is used to cancel votes with guaranteeing privacy and universal verifiability.

A Study on the Subjective Symptoms of the Printers in Chunbuk Area (전북지역 인쇄공의 심신 자각증상 조사연구)

  • 김성숙;유은주;이종섭
    • Journal of Environmental Health Sciences
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    • v.17 no.2
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    • pp.67-77
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    • 1991
  • The purpose of this study was to investigate the psychosomatic health status of printers. The 77 printers and 24 control group were analysed about salary, drinking, smoking, education, sex, marriage, age and working age by the THI (Todai Health Index) questionaire. THI was modified from CMI(Cornell Medical Index) and developed by Tokyo University Research Team in Japan. The resuts obtained were summarized as follows. 1. The printers, who get more salary showed high score about mental conplaints, especially, mental irritability(j), nervusness (E), lie Scale(L), aggressiveness(F) and irregualr life(G) and lower salary showed generally high score about physical complaints, especially, mouth and anus (D), digestive symptom(C) multiple subjective symptom(I). 2. According to the printers drinking amount shows the difference, eg nondrinker scored higher on mouth and anus(D), 90mg/week drinker scored higher on multiple subjective symptom(I), digestive symptom(C), depression(K), nervousness(E), and irregular life(G), 91~179mg/week drinker scored higher on impulsiveness(H), mental irritability(J), 270~359mg/week drinker scored higher on respiratory(A), lie scale (L) and aggressiveness (F). 3. The nonsmoker scored high level on mouth and anus(D), mental irritability(J). The previous smoker scored on multiple symptom(I), eyes and skin(B), digestive(C), lie scale(L), and depression(K). The present smoker scored on respiratory(A), impulsivehess(H), aggressiveness(F), nervousness(E), and irregular life(G). 4. According to the printers working age showed almost high score about subjective symptoms on 1~3 year. 5. Men printers high scored on respiratory(A). lie scale(L), aggressiveness(F), women printers scored about mental complaints, especially, impulsiveness(H), mental irritability(J), depression (K), nervousness (E). 6. According to the printers age showed high scored about, below 20 years were lie scale(L). aggressiveness(F), irregular life(G) 21~30years were multiple subjective symptom(I) respiratory (A), eyes and skin(B), mouth and anus(D), impulsiveness(H), mental irritability(J), depression (K), nervousness(E), and over 41 years were digestive(C). 7. Married printers scored high level on eyes and skin(B), digestive(C) and impulsivehess(H), and single printers on respiratory(A), mouth and anus(D), lie scale(L), mental irritability(J). 8. According to education shows the difference, eg high school scored higher on eyes and skin (B), mental irritability(J), depression(K), nervousness(E), collage and over scored higher on multiple subjective symptom(I ), respiratory (A), mouth and anus (D), lie scale (L), aggressiveness (F), irregular life (G), and middle school scored high on digestive (C), impulsiveness (H).

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Effect of Trisodium Phoshate and Cetylpyridinium chloride on E. coli O157:H7 and Listerial monocytogenes (Trisodium Phoshate와 Cetylpyridinium chloride의 E. coli O157:H7 및 Listerial monocytogenes 에 대한 살균 작용)

  • 강길진
    • Journal of Food Hygiene and Safety
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    • v.13 no.4
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    • pp.365-369
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    • 1998
  • The in vitro effects of trisodium phosphate and cetylpyridinium chloride on E. coli 0157:H7 and L. monocytogenes were investigated. The trisodium phosphate and cetylpyridinium chloride was bactericidal toward E. coli 0157:H7 and L. monocytogenes. The killing effects of the $1{\times}10^{-2}\;M$ trisodium phosphate on E. coli 0157:H7 and L. monocytogenes were 30~40%, 40~50%, respectively. The killing effects of the $5{\times}10^{-7}\;M$ cetylpyridinium chloride on E. coli 0157:H7 and L. monocytogenes were 90~95%, 95~99%, respectively. The killing effects of the trisodium phosphate was $10^5$ times that of the cetylpyridinium chloride. Factors effecting the bactericidal action of trisodium phosphate and cetylpyridinium chloride were investigated and the action depended on temperature and pH.

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D.C. Motor Speed control Using Explicit M.R.A.C. Algorithms (Explicit M.R.A.C. 알고리즘을 이용한 직류 전동기 속도 제어)

  • Kim, Jong-Hwan;Park, Jun-Ryeol;Choe, Gye-Geun
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.20 no.6
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    • pp.11-17
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    • 1983
  • In this paper, the application of the explicit M.R.A.C. algorithms to the D.C. motor speed control using the microprocessor is studied. The adaptation algorithms are derived from the gradient method and the exponentially weighted least square [E.W.L.S.] method. In order to minimize the computational instability of the E.W.L.S. method, the adaptation algorithm of UDUt factorization method is developed, and because of the characteristics of the D.C. motor (dead-aone phenomenon) , the SM. gra-dient type algorithm is also improved from the gradient type algorithm. Computer simulations and experiments show that these algorithms adapt well to the rapid change of the reference input and the load.

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Effect of Temperature and Bake-out on Formaldehyde Emission from UF Bonded Wood Composites

  • Lee, Young-Kyu;Kim, Hyun-Joong
    • Journal of the Korean Wood Science and Technology
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    • v.40 no.2
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    • pp.91-100
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    • 2012
  • This study analysis the effect of various temperatures (20, 35 and $50^{\circ}C$) on the formaldehyde emission from wood composites, which were particleboard (PB), medium density fiberboard (MDF), high density fiberboard (HDF) and laminated HDF (L-HDF) by Japanese desiccator method. Also, to reduce formaldehyde emission by wood composites, it has been suggested that undergo a bake-out conditions. On average, the level of formaldehyde emission increased many times with a $15^{\circ}C$ increase in temperature from 20 to $35^{\circ}C$ for PB, MDF, HDF and L-HDF, respectively. Formaldehyde emissions from wood composites could be expected to increase with increasing ambient temperature. At $35$ for 28 days bake-out treatment of boards, the free formaldehyde emission reduced 67.8% (PB), 40.1% (MDF), 37.8% (HDF), and 35.2% (L-HDF). On the other hand, after the bake-out at $50^{\circ}C$ for 28 days, the formaldehyde concentration decreased by 88.2, 66.9, 62.2 and 59.3% of the concentration before the bake-out for PB, MDF, HDF and L-HDF, respectively. An interesting of the bake-out treatment at $50^{\circ}C$ after 14 days, formaldehyde emission grade of PB & MDF down $E_2$ to $E_1$, and HDF & L-HDF down $E_1$ to $E_0$.

Effect of Chlorine Dioxide and Commercial Chlorine Sanitizer on Inhibiting Foodborne Pathogens and on Preventing the Formation of Chemically Injured Cells on Radish Sprouts

  • Choi, Mi-Ran;Kang, Dong-Hyun;Heu, Sung-Gi;Lee, Sun-Young
    • Food Quality and Culture
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    • v.3 no.1
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    • pp.34-39
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    • 2009
  • This study assessed the efficacy of aqueous chlorine dioxide ($ClO_2$) and commercial chlorine sanitizer in terms of its ability to eliminate Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157:H7 on radish sprouts (Raphanus sativus L.). Radish sprouts were inoculated with a cocktail containing one each of three strains of three different foodborne pathogens, then treated with distilled water (control) or chemical sanitizers (100 ppm commercial chlorine, and 50, 100, 200 ppm $C1O_2$) for 1, 5, and 10 min at room temperature ($22{\pm}2^{\circ}C$). Populations of S. Typhimurium, E. coli O157:H7 and L. monocytogenes were counted at 4.64, 6.05, and 4.29 log CFU/g, respectively, after inoculation. Treatment with water did not significantly reduce the levels of any of the three foodborne pathogens. The levels of all three pathogens were reduced by treatment with chemical sanitizers; however, the observed levels of reduction of E. coli O157:H7 and L. monocytogenes were not significant as compared with the controls. The levels of the three pathogens were reduced most profoundly when treated for 10 min with 200 ppm of $C1O_2$, and the reduction levels of S. Typhimurium, E. coli O157:H7, and L. monocytogenes were 1.17, 1.63, and 0.96 log CFU/g, respectively. When chemically injured cells were investigated using SPRAB for E. coli O157 :H7 and by selective overlay methods for S. Typhimurium and L. monocytogenes, respectively, it was noted that commercial chlorine sanitizer generated more numbers of injured pathogens than did $C1O_2$. These data indicate that $C1O_2$ treatment may prove useful in reducing the numbers of pathogenic bacteria in radish sprouts.

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Growth-inhibiting Effects of Juniperus virginiana Leaf-Extracted Components toward Human Intestinal Bacteria

  • Kim, Moo-Key;Kim, Young-Mi;Lee, Hoi-Seon
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.164-167
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    • 2005
  • The growth responses of materials extracted from Juniperus virginiana leaves against Bifidobacterium bifidum, B. longum, Clostridium perfringens, Escherichia coli, Lactobacillus acidophilus, L. casei, and Streptococcus mutans were examined using impregnated paper disk agar diffusion. The biologically active constituent isolated from the J. virginiana extracts was characterized as ${\alpha}$-cedrene using various spectroscopic analyses including IR, EI-MS, and NMR. The responses varied according to the dose, chemicals, and bacterial strain tested. Methanol extracts of J. virginiana leaves exhibited a strong and moderate inhibitory activity against C. perfringens and E. coli at 5 mg/disk, respectively. However, in tests conducted with B. bifidum, B. longum, L. acidophilus, L. casei, and S. mutans, the methanol extracts showed no or weak inhibitory response. At 2 mg/disk, a-cedrene strongly inhibited the growth of C. perfringens and moderately inhibited the growth of E. coli and S. mutans, without any adverse effects on the growth of four lactic acid-bacteria. Of the commercially available compounds originating from J. virginiana leaves, cedrol and ${\alpha}$-pinene exhibited strong and moderate growth inhibition against C. perfringens, and ${\alpha}$-copaene revealed moderate growth inhibition against E. coli at 1 mg/disk. Furthermore, cedrol exhibited moderate and weak growth inhibition against S. mutans at 2 and 1 mg/disk, respectively. However, little or no activity was observed for camphene, (+)-2-carene, p-cymene, limonene, linalool, and a-phellandrene against B. bifidum, B. longum, C. perfringens, L. acidophilus, L. casei, and S. mutans at 2 mg/disk. The observed inhibitory activity of the J. virginiana leaf-extracted materials against C. perfringens, E. coli, and S. mutans may be an indication of at least one of the pharmacological actions of the J. virginiana leaf.