• Title/Summary/Keyword: dwarf type

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Genetic Differentiation of Phytoplasma Isolates by DNA Heteroduplex Mobility Assay and Single-Strand Conformation Polymorphism Analysis

  • Cha, Byeongjin;Han, Sangsub
    • The Plant Pathology Journal
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    • v.18 no.6
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    • pp.308-312
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    • 2002
  • Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf(MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100% homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.

Varietal Differences in Agronomic Characters under Different Altitudinal Locations with Equal Latitude in Paddy Rice (동일위도상 표고의 차이에 따른 수도품종의 수량형질변이)

  • Park, S.Z.;Lee, E.W.;Lee, B.W.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.164-172
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    • 1983
  • The present study was conducted to investigate the variations in morphological traits, yield and yield-related components of rice cultivars exposed to different weather conditions by growing at the locations with different altitude but with equal latitude. Three Japonica varieties (Daegoldo, Jinheung, Shin #2) and three Tongil type semi-dwarf varieties(Josaengtongil, Suweon 287, wx 817-65-2-3) were seeded at Suweon on April 21 and transplanted at Suweon ($127^{\circ}$ N37 20', altitude 37m) and Jechun ($128.2^{\circ}$, N37 10', altitude 280-300m) on June 1 with the spacing of 30 $\times$ 15 em. The morphological traits, yield and yield-related components were measured. Culm length, paincle length and 1000-grain weight showed a little increase in Japonica varieties in Jechun as compared to those in Suweon and vice versa in semi-dwarf varieties, showing no significant differences. Number of panicles, per hill and number of spikelets per panicle among yield-related components showed remarkable increase in all varieties in Jechun as compared to those in 'Suweon but such inter-locational differences differed with varieties within varietal group and between varietal groups. On the contrary, grain fertility was higher in Suweon than that in Jechun and in Japonica varieties than that in semi-dwarf varieites , showing greater inter-locational difference in semi-dwarf varieties. Among Japonica varieties Jinheung and Shin #2 showed remarkable increase in yield in Jechun as compared to that in Suweon but in semidwarf varieties it was just the opposite. The importance of each of the yield-related component contributing to yield showed similar tendency 'regardless of locations in Japonica varieties. However, it was evidently different between locations in semidwarf varieties, where number of spikelets per panicle showed greatest direct effect on yield in Suweon but in Jechun so did grain fertility followed by the number of spikelets per panicle.

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Korean-Japanese Planet Search Program: Search for Planets around G-type Giants

  • Omiya, Masashi;Han, In-Woo;Izumiura, Hideyuki;Lee, Byeong-Cheol;Sato, Bun'ei;Kim, Kang-Min;Yoon, Tae-Seog;Kambe, Eiji;Yoshida, Michitoshi;Masuda, Seiji;Toyota, Eri;Urakawa, Seitaro;Takada-Hidai, Masahide
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.1
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    • pp.48.2-48.2
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    • 2010
  • Korean-Japanese Planet Search Program has been carried out since 2005 to search for planets around intermediate-mass giant stars (1.5-5.0 solar masses) by an international collaboration between Korean and Japanese researchers. In this program, we have been carrying out a precise radial velocity survey of about 190 G-type giant stars (6.21.9 solar masses) giant stars. These results extend the planet mass distribution of massive intermediate-mass stars to higher and lower mass region, and may further constrain substellar system formation mechanisms. We report the recent results and current status of Korean-Japanese Planet Search Program.

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Independent Inheritance between df2 gene and ti gene in Soybean

  • Han, Eun-Hui;Sung, Mi-Kyung;Kim, Kyung-Roc;Park, Jung-Soo;Nam, Jin-Woo;Chung, Jong-Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.14-17
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    • 2011
  • Dwarfuess and Kunitz trypsin inhibitor (KTI) protein in soybean is useful traits for basic studies. df2 and ti gene control dwarfness and the expression of Kunitz trypsin inhibitor (KTI) protein in soybean, respectively. The objective of this research was to verify genetic linkage or independent inheritance of df2 and ti loci in soybean. The $F_2$ population was made by cross combination between "Gaechuck#2" (Df2Df2titi genotype, KTI protein absence and a normal growth type) and T210 (df2df2TiTi genotype, a dwarf growth type and KTI protein present). A total of 258 $F_2$ seeds were analyzed for the segregation of KTI protein using SDS-PAGE. And so, 198 $F_2$ plants were recorded for the segregation of dwarfness. The segregation ratio of 3 : 1 for Ti locus (201 Ti_ : 57 titi) and Df2 locus (143 Df2_ : 55 df2df2) was observed. Segregation ratio of 9 : 3 : 3 : 1 (116 Ti_Df2_: 44 Ti_df2df2: 27 titiDf2_: 11 titidf2df2) between df2 gene and ti gene was observed ($x^2$=3.53, P = 0.223). These results showed that df2 gene was inherited independently with the ti gene in soybean.

Newly developed MSAP analysis reveals the different polymorphism patterns in transgenic tobacco plants with the dsRNA MET1 gene

  • Oh, Yun Jung;Chung, Hee;Yu, Jae Gyeong;Park, Young Doo
    • Plant Biotechnology Reports
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    • v.3 no.2
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    • pp.139-145
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    • 2009
  • DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. In this study, we isolated NtMET1 from Nicotiana tabacum cv. Havana (SR1) and obtain transgenic plants that reduced MET1 expression level with the double-strand RNA (dsRNA) MET1 gene. Transgenic tobacco plants showed dwarf and abnormal flower development when compared with the wild type. Using methylation-sensitive amplified polymorphism (MSAP) analysis, the patterns of cytosine methylation in transformed plants and the wild type were compared. MseI/HpaII selection primers showed an interesting polymorphism, and 153 DNA bands of interest were detected. Among these, 30 selective fragments were sequenced and analyzed with a BLAST search by successful MSAP modifications. The homology search showed that the transposons and tandem repeated sequences were related to the phenotypes. These results suggested that the decreased degree of methylation by dsRNA strategy caused abnormal growth and development in N. tabacum.

Cataclysmic Variables as Supernova Ia Progenitors

  • Kafka, Stella
    • Journal of Astronomy and Space Sciences
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    • v.29 no.2
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    • pp.163-167
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    • 2012
  • Although the identification of the progenitors of type Ia supernovae (SNeIa) remains controversial, it is generally accepted that they originate from binary star systems in which at least one component is a carbon-oxygen white dwarf (WD); those systems are grouped under the wide umbrella of cataclysmic variables. Current theories for SNeIa progenitors hold that, either via Roche lobe overflow of the companion or via a wind, the WD accumulates hydrogen or helium rich material which is then burned to C and O onto the WD's surface. However, the specifics of this scenario are far from being understood or defined, allowing for a wealth of theories fighting for attention and a dearth of observations to support them. I discuss the latest attempts to identify and study those controversial SNeIa progenitors. I also introduce the most promising progenitor in hand and I present observational diagnostics that can reveal more members of the category.

A STUDY ON ABUNDANCE INDICATOR FOR LATE TYPE DWARFS

  • Lee, Sang-Gak
    • Journal of The Korean Astronomical Society
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    • v.16 no.2
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    • pp.55-63
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    • 1983
  • From UBVRI photometry and space motion data of 232 late type dwarf stars, it is found that for the stars of (R-I)<0.5, $\Delta$(U-B) and $\Delta$(B-V) color excesses are correlated with their orbital eccentricities. Therefore, $\Delta$(U-B) and $\Delta$(B-V) color excesses can be used as possible photometric abundance indicators for the stars of (R-I)<0.5. For the stars of (R-I)${\geq}$0.5, the correlation between color excess and orbital eccentricity is not clear. However, it is interesting to note that the high orbital eccentricity stars show some blue deficiencies and these blue deficiencies seem to be correlated with orbital eccentricity.

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Vegetation Characteristics of Main Herbaceous Flowers for Ecological Restoration (생태복원용 주요 초화류의 녹화특성)

  • Shim, Sang-Ryul;Kim, Jae-Hwan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.9 no.1
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    • pp.64-71
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    • 2006
  • Research was initiated to investigate vegetation coverage and growth of main herbaceous flowers under low maintenance condition like a wild state. Eleven entries were comprised of 1 annual, 2 biennials and 8 perennials from widely used herbaceous flowers in Korea. Vegetation coverage and germination rate were high on annual and biennials when compared to perennials. Callistephus chinensis showed high vegetation coverage but lasted only for a year in 2001 and disappeared in 2002 as an annual. Oenothera odorata and Oenothera lamarckiana were also high vegetation coverage species with persistent only for two years as biennials. Therefore, these species should not be used in a large amount for ecological restoration when mixed with perennials because of affecting the vegetation coverage of perennials. There were considerable variations in vegetation coverage with perennial herbaceous flowers : Lotus corniculatus and Taraxacum platycarpum-high vegetation coverage species, Platycodon grandiflorum, Aster tataricus and Aster koraiensis-medium vegetation coverage species, and Aster yomena, Patrinia scabiosaefolia and Chrysanthemum indicum-low vegetation coverage species. Growth types of herbaceous flowers tested in this research were divided into 4 types : Callistephus chinensis, Oenothera lamarckiana and Aster koraiensis-wide leaf and tall type, Aster tataricus, Patrinia scabiosaefolia, Oenothera odorata, Taraxacum platycarpum, and Aster yomena-medium leaf and medium tall type, Platycodon grandiflorum-fine leaf and dwarf type, and Lotus corniculatus-fine leaf and tall type.

SNR 0104-72.3: A remnant of Type Ia Supernova in a Star-forming region?

  • Lee, Jae-Jun;Park, Sang-Wook;Hughes, John P.;Slane, Patrick;Burrows, David
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.1
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    • pp.87.2-87.2
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    • 2011
  • We report our 110 ks Chandra observations of the supernova remnant (SNR) 0104-72.3 in the Small Magellanic Cloud (SMC). The X-ray morphology shows two prominent lobes along the northwest-southeast direction and a soft faint arc in the east. Previous low resolution X-ray images attributed the unresolved emission from the southeastern lobe to a Be/X-ray star. Our high resolution Chandra data clearly shows that this emission is diffuse, shock-heated plasma, with negligible X-ray emission from the Be star. The eastern arc is positionally coincident with a filament seen in optical and infrared observations. Its X-ray spectrum is well fit by plasma of normal SMC abundances, suggesting that it is from shocked ambient gas. The X-ray spectra of the lobes show overabundant Fe, which is interpreted as emission from the reverse-shocked Fe-rich ejecta. The overall spectral characteristics of the lobes and the arc are similar to those of Type Ia SNRs, and we propose that SNR 0104-72.3 is the first case for a robust candidate Type Ia SNR in the SMC. On the other hand, the remnant appears to be interacting with dense clouds toward the east and to be associated with a nearby star-forming region. These features are unusual for a standard Type Ia SNR. Our results suggest an intriguing possibility that the progenitor of SNR 0104-72.3 might have been a white dwarf of a relatively young population.

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In vitro Variant Induction and Its Content of Gentiopicroside of Gentiana scabra BUNGE (용담(龍膽)의 기내변이주(器內變異株) 유도(誘導)와 변이주(變異株)의 Gentiopicroside 함량(含量))

  • Seong, Nak-Sul;Park, Chung-Heon;Kim, Kwan-Su;Lee, Seoung-Tack;Chang, Yeong- Hee
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.1
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    • pp.40-44
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    • 1995
  • This study was aimed to mutant induction in Gentiana scabra BUNGE through the tissue culture techniques and comparison of gentiopicroside content in each mutant plants derived from tissue culture. In order to induce the mutants of Gentiam scabra, seed, apical and lateral bud were soaked in $NaN_3$, EMS and MNU solutions and cultured on MS basal medium containing 0.5mg/l NAA, BA $NaN_3$, MNU-treated buds survived about 50% but not survived in EMS treatment. Seed germination rate was extremely low in EMS and MNU treatments but various types of mutant plantlets were obtained by those treatments. 63% of elongated type and 37% of dwarf type were obtained from tissue culture treated by various mutagen. Gentiopicroside contents of regenerated plantlets was analyzed. Root of dwarf type contained more gentiopicroside(1.383%) than that of elongation type (0.083%).

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