• The Korean Journal of Pesticide Science
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• v.2 no.3
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• pp.79-84
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• 1998

Tandem HPLC and atmospheric pressure chemical ionization(APcI) LC/MS method was used for the determination and confirmation of carbendazim residues in soybean sprout. Fluorescence(FL) detector was connected in tandem with the ultraviolet(UV) detector for dual detection of the carbendazim residue at the excitation and emission wavelength of 280 nm and 310 nm, respectively. The limit of detection for carbendazim was $0.1{\mu}g/kg$ sample. Recoveries of carbendazim from fortified soybean sprout at 0.5, 1.0 and 2.0 ppm were averaged 89.1%. Mass spectrometry using a APcI source confirmed the carbendazim residue in the soybean sprout sample. Fragmentation pattern on the APcI LC/MS spectrum of carbendazim was simpler than that from electron impact(EI) mass spectrum. Carbendazim produced 3 major ions including m/z 133, m/z 159 and m/z 191($M^{+}$). This method was sensitive enough to provide reliable and reproducible results for practical applications.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.7
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• pp.3419-3424
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• 2013

Diverse immunoassays including a chemiluminescent immunoassay (CIA) are used to detect hepatitis B surface antigen (HBsAg) and antibody (anti-HBs). Recently, an increasing number of institutions have been utilizing an immunochromatography assay (ICA), which is easy to use. In this study, We evaluated ICA kits for the rapid detection of HBsAg and anti-HBs by comparing them with a CIA. A total of 120 serum hospital samples, were collected for the whole month, were assayed using ICA kit. The Concordance rate, sensitivity, specificity, positive predictive value and negative predictive value of the ICA for HBsAg based on CIA results were 97%, 97%, 100%, 100%, and 96.8%, respectively. The diagnostic performances of the ICA for Anti-HBs were 90%, 90%, 93.3%, 93.1%, and 90.3%, respectively. The ICA kit failed to detect HBsAg and anti-HBs in low reactive samples. The ICA kits for the rapid detection of HBsAg might be recommended for interpreted with caution and dual analysis in the clinical laboratory.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.1
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• pp.329-337
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• 2012

Considerable attention has been given to the accuracy of HER-2 testing and the correlation between the results of different testing methods. This interest reflects the growing importance of HER-2 status in the management of patients with breast cancer. In this study the detection of HER-2 gene and centromere 17 status was evaluated using dual-colour primed in situ labelling (PRINS) in comparison with fluorescence in situ hybridization (FISH). These two methods were evaluated on a series of 27 formalin fixed paraffin embedded breast carcinoma tumours, previously tested for protein overexpression by HercepTest (grouped into Hercep 1+/0, 2+ and 3+). HER-2 gene amplification (ratio${\geq}2.2$) by PRINS was found in 3:3, 6:21 and 0:3 in IHC 3+, 2+ and 1+/0 cases, respectively. Comparing FISH and IHC (immunohistochemistry), showed the same results as for PRINS and IHC. Chromosome 17 aneusomy was found in 10 of 21 IHC 2+ cases (47.6%), of which 1 (10%) showed hypodisomy (chromosome 17 copy number per cell${\leq}1.75$), 7 (70%) showed low polysomy (chromosome 17 copy number per cell=2.26 - 3.75) and 2 (20%) showed high polysomy (chromosome 17 copy number per cell ${\geq}3.76$). The overall concordance of detection of HER-2 gene amplification by FISH and PRINS was 100% (27:27). Furthermore, both the level of HER-2 amplification and copy number of CEN17 analysis results correlated well between the two methods. In conclusion, PRINS is a reliable, reproducible technique and in our opinion can be used as an additional test to determine HER-2 status in breast tumours.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.6C
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• pp.626-633
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• 2009

ISO/IEC/WD 24753 defines new modem specifications for a long-range RFID communications and application protocol for a sensor tag system. According to the standard, the frequency offset of the tag is 4%. In general wireless communications systems, it is known that a coherent receiver is superior to a non-coherent receiver. However, if the frequency offset is large, it is difficult to restore the original data accurately with a coherent receiver, and the performance of a coherent receiver is easily degraded. In this paper, a non-coherent receiver structure is adopted to solve the frequency offset problem of long-range RFID communications. We designed a frequency estimation block to find an optimal frequency from the received signal with 4% frequency offset and proposed a start frame delimiter (SFD) detection algorithm to determine the start position of the payload. The frequency estimation block finds the optimal frequency from the received signal using 9-correlators. And the SFD detection block searches the received signal to find the start position of the payload with dual correlator. We implemented a long-range RFID reader with the proposed methods and evaluated its performance in a wired/wireless test network. The implemented long-range RFID reader showed more superior performance than the commercial RFID reader in terms of recognition range.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.36.2-36.2
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• 2015

We report the first detection of linearly polarized emission at an observing wavelength of 350 mum from the radio-loud active galactic nucleus 3C 279. We conducted polarization observations for 3C 279 using the SHARP polarimeter in the Caltech Submillimeter Observatory on 2014 March 13 and 14. For the first time, we detected the linear polarization with the degree of polarization of $13.3%{\pm}3.4%$ (3.9sigma) and the electric vector position angle (EVPA) of $34.^{\circ}7{\pm}5.^{\circ}6$. We also observed 3C 279 simultaneously at 13, 7, and 3.5 mm in dual polarization with the Korean very long baseline interferometry (VLBI) Network on 2014 March 6 (single dish) and imaged in milliarcsecond (mas) scales at 13, 7, 3.5, and 2.3 mm on March 22 (VLBI). We found that the degree of linear polarization increases from 10% to 13% at 13 mm to 350 mum and the EVPAs at all observing frequencies are parallel within < $10^{\circ}$ to the direction of the jet at mas scale, implying that the integrated magnetic fields are perpendicular to the jet in the innermost regions. We also found that the Faraday rotation measures RM are in a range of $-6.5{\times}102{\sim}-2.7{\times}103$ rad m-2 between 13 and 3.5 mm, and are scaled as a function of wavelength:| {RM}| ${\backslash}propto$ {lambda }-2.2. These results indicate that the millimeter and sub-millimeter polarization emission are generated in the compact jet within 1 mas scale and affected by a Faraday screen in or in the close proximity of the jet.

• Journal of the Korean Society for Nondestructive Testing
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• v.29 no.5
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• pp.466-472
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• 2009

An acoustic leak monitoring system(ALMS) using acoustic emission(AE) technique was applied for leakage detection of nuclear power plant's pipeline which is operated in high temperature and pressure condition. Since this system only monitors the existence of leak using the root mean square(RMS) value of raw signal from AE sensor, the difficulty occurs when the characteristics of leak size and shape need to be evaluated. In this study, dual monitoring system using AE sensor and accelerometer was introduced in order to solve this problem. In addition, artificial neural network(ANN) with Levenberg.Marquardt(LM) training algorithm was also applied due to rapid training rate and gave the reliable classification performance. The input parameters of this ANN were extracted from varying signal received from experimental conditions such as the fluid pressure inside pipe, the shape and size of the leak area. Additional experiments were also carried out and with different objective which is to study the generation and characteristic of lamb and surface wave according to the pipe thickness.

• Proceedings of the Korea Society of Information Technology Applications Conference
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• 2005.11a
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• pp.325-329
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• 2005

Vast amount of information is generated and shared in this active digital As the digital informatization is vividly going on now, most of documents are in digitalized forms, and this kind of information is on the increase. It is no exaggeration to say that this kind of newly created information and knowledge would affect the competitiveness and the future of our nation. In addition to that, a lot of investment is being made in information and knowledge based industries at national level and in reality, a lot of efforts are intensively made for research and development of human resources. It becomes easier in digital era to create and share the information as there are various tools that have been developed to create documents along with the internet, and as a result, the share of dual information is increasing day in and day out. At present, a lot of information that is provided online is actually being plagiarized or illegally copied. Specifically, it is very tricky to identify some plagiarism from tremendous amount of information because the original sentences can be simply restructured or replaced with similar words, which would make them look different from original sentences. This means that managing and protecting the knowledge start to be regarded as important, though it is important to create the knowledge through the investment and efforts. This dissertation tries to suggest new method and theory that would be instrumental in effectively detecting any infringement on and plagiarism of intellectual property of others. DICOM(Dynamic Incremental Comparison Method), a method which was developed by this research to detect plagiarism of document, focuses on realizing a system that can detect plagiarized documents and parts efficiently, accurately and immediately by creating positive and various detectors.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 1998.06b
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• pp.92-97
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• 1998

For the efficient searching and browsing of digital video, it is essential to extract the internal structure of the video contents. As an example, a news video consists of several sections such as politics, economics, sports and others, and also each section consists of individual topics. With this information in hand, users can ore easily access the required video frames. This paper addresses the problem of automatic shot boundary detection and selection of representative frames (R-frames), which are the essential step in recognizing the internal structure of video contents. In the shot boundary detection, a new algorithm that have dual detectors which are designed specifically for the abrupt boundaries (cuts) and gradually changing bounaries respectively is proposed. Compared to the existing 미algorithms that mostly have tried to detect both types by a single mechanism, the proposed algorithm is proved to be more robust and accurate. Also in the problem of R-frame selection, simple mechanical approaches such as selecting one frame every other second have been adopted. However this approach often selects too many R-frames in static short, while drops important frames in dynamic shots. To improve the selection mechanism, a new R-frame selection algorithm that uses motion information extracted from pixel difference is proposed.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.3
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• pp.225-231
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• 2011

The satellite clock anomalies, one of the abnormal signal factors of the GPS satellites, can have a significant impact on the GPS measurements. However, it can be difficult to detect the anomalies of the satellites clock before the range of the satellites clock error becomes bigger than the range of the other factors, due to the measurement including error of the orbit, ionosphere delay, troposphere delay, multipath and receiver clock. In order to perform quick and accurate detection by minimization of critical range in anomalies of the satellites clock, this paper suggested a solution to detect precise anomalies of the satellites clock after application of carrier smoothing filter from measurement by dual-frequency and adjustment of errors which can be occurred by other factor and the receiver clock errors. The performance of the proposed method was confirmed by comparing to the satellite clock biases which are provided by IGS.

• Journal of Microbiology
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• v.42 no.2
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• pp.126-132
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• 2004

A single chain variable fragment (scFv) specific towards B. pseudomallei exotoxin had previously been generated from an existing hybridoma cell line (6E6AF83B) and cloned into the phage display vector pComb3H. In this study, the scFv was subcloned into the pComb3X vector to facilitate the detection and purification of expressed antibodies. Detection was facilitated by the presence of a hemagglutinin (HA) tag, and purification was facilitated by the presence of a histidine tag. The culture was grown at 30$^{\circ}C$ until log phase was achieved and then induced with 1 mM IPTG in the absence of any additional carbon source. Induction was continued at 30$^{\circ}C$ for five h. The scFv was discerned by dual processes-direct enzyme-linked immunosorbent assays (ELISA), and Western blotting. When compared to E. coli strains ER2537 and HB2151, scFv expression was observed to be highest in the E. coli strain Topl0F'. The expressed scFv protein was purified via nickel-mediated affinity chromatography and results indicated that two proteins a 52 kDa protein, and a 30 kDa protein were co-purified. These antibodies, when blotted against immobilized exotoxin, exhibited significant specificity towards the exotoxin, com-pared to other B. pseudomallei antigens. Thus, these antibodies should serve as suitable reagents for future affinity purification of the exotoxin.