• Title/Summary/Keyword: dual cycle

Search Result 241, Processing Time 0.022 seconds

Structural Similarity and Expression Differences of Two Pj-Vg Genes from the Pandalus Shrimp Pandalopsis japonica

  • Jeon, Jeong-Min;Kim, Bo-Kwang;Kim, Young-Ji;Kim, Hyun-Woo
    • Fisheries and Aquatic Sciences
    • /
    • v.14 no.1
    • /
    • pp.22-30
    • /
    • 2011
  • Vitellogenin (Vg) is the precursor of vitellin (Vn), which is the major yolk protein in nearly all oviparous species, including fish, amphibians, reptiles, and most invertebrates. It is one of the most important factors during reproduction, and numerous studies have shown that Vg genes are markers of the reproductive cycle and effecter genes induced by endocrine-disrupting chemicals (EDCs). Previously, we isolated two distinct cDNAs encoding vitellogenin homologs Pj-Vg1 and Pj-Vg2 from Pandalus shrimp Pandalopsis japonica. In this study, full-length genomic sequences of Pj-Vg1 and Pj-Vg2 were determined using a PCR-based genome walking strategy. Isolated Pj-Vg1 and Pj-Vg2 genes were 11,910 and 11,850 bp long, respectively. Both Pj-Vg genes had 15 exons and 14 introns, and the splicing sites were also the same, suggesting that they arose via gene duplication. The similar structural characteristics of decapod Vg genes suggest that they are all orthologs that evolved from the same ancestral gene. Analysis of Pj-Vg1 and Pj-Vg2 expression revealed that the relative copy numbers of Pj-Vg1 and Pj-Vg2 were similar in the hepatopancreas, whereas Pj-Vg2 transcripts were also detected in the ovary. Expression of both Pj-Vg genes was induced in hepatopancreas of mature individuals, whereas only Pj-Vg2 transcripts were upregulated in the ovaries from mature animals, suggesting that both Pj-Vgs are important for oocyte development. A strong positive correlation was found between Pj-Vg1 and Pj-Vg2 transcripts in the same individual, indicating they are under the same control mechanisms. Additionally, a positive correlation was found between ovarian and hepatopancreatic Pj-Vg2 transcripts, suggesting that its dual expression is regulated by similar physiological conditions. Knowledge of the similarities and differences between the two vitellogenin-like genes, Pj-Vg1 and Pj-Vg2, would help us to understand their roles in reproduction and other physiological effects.

A Study of Factors Affecting Bone Mineral Density in Children: Anthropometric Measurements, Socioeconomic Factors, Family History, and Other Environmental Factors (성장기 아동의 골밀도에 영향을 주는 요인에 관한 연구: 신체계측치, 사회경제적 요인, 가족력 및 기타 환경요인)

  • 박정난;김경희;이상선
    • Journal of Nutrition and Health
    • /
    • v.37 no.1
    • /
    • pp.52-60
    • /
    • 2004
  • This study was conducted to investigate the bone mineral density (BMD) and the anthropometric measurements, socioeconomic factors, family history of osteoporosis and other environmental factors affecting BMD in children. One hundred sixty children (80 males, 80 females) in second grade of elementary school in Seoul were recruited; the mean age was 7.7 $\pm$ 0.47 years. Bone mineral density was measured in the lumbar spine (LS), femoral neck (FN), femoral trochanter (FT), and Ward's triangle (WT) by Dual Energy X-ray Absorptiometry (DEXA). Socioeconomic factors, family history of osteoporosis, and other environmental factors were assessed by questionnaire. Bone mineral densities of LS, FN, FT, and WT were 0.677, 0.637, 0.618, and 0.658 g/$\textrm{cm}^2$. BMD of boys was higher than that of girls (LS: 0.685 vs. 0.669, FN: 0.660 vs. 0.614, FT: 0.632 vs. 0.604, WT: 0.678 vs. 0.639 g/$\textrm{cm}^2$). Anthrometric measurements (height, weight relative body weight, circumference of waist and hip) were positively correlated with BMD. With increasing family income, BMD of LS was significantly lowered, and negative correlation was observed between mother's age at birth of subject and the BMD of FN. And the BMD of children with a family history of osteoporosis was significantly lower. However BMDs were not different by educational level of parents, family pattern, delivery term, birth weight, and type of feeding. Further studies are needed to clarify the factors affecting BMD of children and earlier age, included infants and even prenatal life. If any association is revealed and persist until the attainment of peak bone mass, osteoporosis prevention programs are needed to be start very early in the life cycle.

Anatomy, Biomechanics and Physical Examination of Foot and Ankle (족부 및 족관절의 해부학, 생역학 및 신체 검사)

  • Hur, Chang-Yong;Kim, Hak Jun
    • The Journal of Korean Orthopaedic Ultrasound Society
    • /
    • v.3 no.1
    • /
    • pp.32-37
    • /
    • 2010
  • Ultrasonographic diagnosis and treatment in Orthopaedic fields had been widely used. Sonographic diagnosis and treatment of foot and ankle is convenient because of anatomical characteristics. The knowledge of the anatomy and biomechanics in foot and ankle area can help to diagnose and treat the disease around foot and ankle. 28 bone and many tendons, ligaments and muscles are consist of ankle and foot joint and the coordinative relation among these structures can allow the dual function, weight bearing and locomotion of ankle and foot during gait cycle. Foot and ankle have small room for many structures, so systemically physical examination is essential for diagnosis. Accurate understanding of foot and ankle anatomy and biomechanics could be helpful to using ultrasonograph.

  • PDF

A Study on Family Life Education (가족생활 교육 프로그램 개발을 위한 기초연구 (I))

  • 유영주;오윤자
    • Journal of Families and Better Life
    • /
    • v.8 no.2
    • /
    • pp.49-68
    • /
    • 1990
  • Family Life Education(FLE) is an educational specialty which was originated in reaction to changing social conditions, industrialization, and urbanization It deals with the perceived inadequacies of families to cope with these changes, thereby reducing social problems involved with and improving family life. With an assumption that the studies about FLE have not been so active in Korea, the present thesis examines the FLE in Korea; i.e. the definition, the objectives, the scope, the approximation of family life education, and the necessity of marital education in Korea based upon the published theories of FLE in th United Stated of America. Also it attempts to formulate a tentative plan for the promotion of a FLE program in Korea. The concept of FLE in Korea was manifested in the social educational law and life-span education, specified as the 4th among ten presidential regulations; and it has drawn an institutional, governmental attention It is defined as " the life-span developmental education to enhance the quality of human life, to solve family problem, to develop an individual's potentiality, and to strengthen the family correlations." of the FLE programs, the marital education is considered one of the crucial subjects because it is the core of the family life. With this premise, FLE tries to support the healthy marital relations, , subsequently helping to explore the family potentiality and to strengthen family ties, Considering the seemingly dual characteristics of the Korean family types, the contents of marital education program should be consisted of the following; marital communication, role cooperation, and effective expressions of mutual affection, In addition, reciprocal understanding and cooperation of the married couples to overcome the differences of value, personality, hobby, and religion, the educational methods in raising kids, and the effective management of home economics should be included. The objects of the FLE program are unmarried, pre-married, and married persons. Fro the married persons, the FLE program should be arranged in accordance with their marital status divided by the family life cycle so that they may prevent possible family problems at each stage of the family life. Also, to prevent the problems incurred in the curse of carrying out family functions, the FLE program should be provided with on the basis of a family unit, thereby improving the quality of the family functions.

  • PDF

The Control Mechanism of Gonadotropin-Releasing Hormone and Dopamine on Gonadotropin Release from Cultured Pituitary Cells of Rainbow Trout Oncorhynchus mykiss at Different Reproductive Stages

  • Kim, Dae-Jung;Suzuki, Yuzuru;Aida, Katsumi
    • Fisheries and Aquatic Sciences
    • /
    • v.14 no.4
    • /
    • pp.379-388
    • /
    • 2011
  • The mechanism by which gonadotropin-releasing hormone (GnRH) and dopamine (DA) control gonadotropin (GTH) release was studied in male and female rainbow trout using cultured pituitary cells obtained at different reproductive stages. The mechanisms of follicle-stimulating hormone (FSH) release by GnRH and DA could not be determined yet. However, basal and salmon-type GnRH (sGnRH)- or chicken-II-type GnRH (cGnRH-II)- induced luteinizing hormone (LH) release increased with gonadal maturation in both sexes. LH release activity was higher after sGnRH stimulation than cGnRH-II stimulation at maturing stages in both sexes. The GnRH antagonist ([Ac-3, 4-dehydro-$Pro^1$, D-p-F-$Phe^2$, D-$Trp^{3,6}$] GnRH) suppressed LH release by sGnRH stimulation in a dose-dependent manner, although the effect was weak in maturing fish. The role of DA as a GTH-release inhibitory factor differs during the reproductive cycle: the inhibition of sGnRH-stimulated LH release by DA was stronger in immature fish than in maturing, ovulating, or spermiated fish. DA did not completely inhibit sGnRH-stimulated LH release, and DA alone did not alter basal LH release. Relatively high doses ($10^{-6}$ or $10^{-5}M$) of domperidone (DOM, a DA D2 antagonist) increased LH release, which did not change with reproductive stage in either sex. The potency of DOM to enhance sGnRH-stimulated LH release was higher in maturing and ovulated fish than in immature fish. These data suggest that LH release from the pituitary gland is controlled by dual neuroendocrine mechanisms by GnRH and DA in rainbow trout, as has been reported in other teleosts. The mechanism of control of FSH release, however, remains unknown.

Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms

  • Kian, Danielle;Lancheros, Cesar Armando Contreras;Damiani, Igor Alexandre Campos;Fernandes, Tamiris Zanforlin Olmos;Pinge-Filho, Phileno;dos Santos, Marcia Regina Machado;da Silveira, Jose Franco;Nakamura, Celso Vataru;da Silva, Joao Santana;Yamada-Ogatta, Sueli Fumie;Yamauchi, Lucy Megumi
    • Parasites, Hosts and Diseases
    • /
    • v.53 no.4
    • /
    • pp.483-488
    • /
    • 2015
  • This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.

Relationship between body fat distribution and menstrual disorder in Obese Pre-menopausal Korean Women (한국 성인 비만여성의 체지방 분포와 월경 장애의 상관성 분석)

  • Choi, Ga-Ya;Song, Mi-Yeon;Shin, Hyun-Dai;Chung, Won-Suk
    • Journal of Korean Medicine for Obesity Research
    • /
    • v.4 no.1
    • /
    • pp.23-31
    • /
    • 2004
  • Background: Obesity is associated with menstrual disorder. Especially, upper-body obesity affects on female reproductive function. Objectives: The goals were to investigate relation between fat distribution and menstrual disorder in obese pre-menopausal Korean females. The hypothesis were tested that there is a relation between upper body obesity and menstrual disorder. Design: A cross-sectional evaluation of 66 Females (baseline age $32.15{\pm}7,32)$ with body mass index $31.22{\pm}4.05\;kg/m^2$. Body composition was measured using bioimpedence analysis (BIA) and anthropometry was done by same observer. VAS and Multidimensional verval rating scale(MVRS) were checked for menstrual pain. Dual-Energy X-ray Absorptiometry (DXA) was measured for evaluating body fat distribution. Menstrual disorder and body fat distribution were compared using statistical methods. Results: TLFR and WH ratio was higher in menstrual cyclic disorder group than controls. There was negative relationship between VAS and TLFR. Conclusions: The data shows that disorder of menstrual cycle was associated with upper body obesity. WH ratio could be one of the factor of menstrual disorder. VAS was correlated with lower body obesity. Further studies for role of upper body fat distribution on female reproductive function and relationship between menstrual pain and fat distribution thought to be needed.

  • PDF

Bicycle Wear Survey Based a Study on the Development of the Commute for Bike Wear Textile Design (바이크복 현황조사를 기반으로 한 출.퇴근용 바이크복 텍스타일 디자인 개발에 관한 연구)

  • Lee, Youn-Soon;Eum, Ji-Eun
    • Journal of the Korea Fashion and Costume Design Association
    • /
    • v.14 no.4
    • /
    • pp.179-190
    • /
    • 2012
  • During recent few years, Bike is arising as one of the most important item of sports market. As well as in domestic markets, customers are demanding specific brands only for young aged riders. Therefore, this study researched current bike wears and suggest digital textile printing with sports wear trends which also have safety and visual impact effectiveness, so it can be satisfied as green products and we also try to suggest sports sensitivity of existing cycle wear to city fashion. The purpose of this study is to figure out 20's-30's female biker attitude for bike wear and their purchasing habits and also their preference, digital textile design of city biker wear based on present condition research of riding wear markets and to establish high quality products with occupying high percentage of global markets and making high profits. For this study, bike wear distributors Survey First, foreign companies generally meet evenly sampling the 20s and 30s targeted vendors 10 companies were selected. 'city sporty ware' or 'casual line' dual 'extreme line' from all manufacturers, including, however did not distinguish it from all the vendors. This higher proportion of the recent upsurge in public and non-professional riders, despite the fact that you can see that the lack of 'Extreme line' compared to the 'casual' line of the city sporty ware production. overall seasonal sales in the spring appeared windbreak jacket, leggings and sweat emissions and at the same time built into the fabric dry faster T-shirt and shorts in the summer sales were higher. autumn jacket and produced excellent warm in winter fleece fabric T-shirts, pants, windshield jacket higher sales. showed improvements in design, improved in the order of the highest and perfect for everyday wear for both. As mentioned, we figure out 20's-30's female rider's attitude for riding suit and their purchasing habits and also their preference, so we can develop riding suit design based on their needs and suggest new design patterns.

  • PDF

Occupational Health Nurses' Role Experiences (산업간호사의 역할 경험)

  • June, Kyung-Ja;Joo, Hea-Ju;Kim, Young-Mi
    • Korean Journal of Occupational Health Nursing
    • /
    • v.20 no.3
    • /
    • pp.250-260
    • /
    • 2011
  • Purpose: The purpose of this study was to describe the role experiences of occupational health nurse. The research question was "what are daily experiences in practice?" Methods: The data were collected through the focus-group interviews with nine occupational health nurses and analyzed by the grounded theory of Strauss and Corbin (1998). Results: The core category on occupational health nurse role experience was named as "struggling alone with the dual role". The causal conditions included 'unfamiliar area' and 'new work'. The contextual conditions included 'overload of work, 'tasks beyond OHN job' and 'disharmony with colleagues'. The intervening conditions were analyzed as supportive factors and discouraging factors. There were action/interaction strategies 'getting information about tasks', 'working as a professional', 'establishing a cooperative relationship', 'seeking help' by trying to regulate the phenomenon. Finally they succeed in 'establishing the identity' and 'feeling proud', but sometimes they could be 'suffering from low morale', or 'considering turnover'. Conclusion: Through these results, it can be explained that occupational health nurses have been exerting themselves to establish their role with various situation through the conflicted context. Based on the findings, we suggest developing the diverse continuing education programs to be tailored for OHN's needs and reinforcing the occupational health services under the legal and political support.

A Design of DLL(Delay-Locked-Loop) with Low Power & High Speed locking Algorithm (저전력과 고속 록킹 알고리즘을 갖는 DLL(Delay-Locked LooP) 설계)

  • 경영자;이광희;손상희
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.26 no.12C
    • /
    • pp.255-260
    • /
    • 2001
  • This paper describes the design of the Register Controlled DLL(Delay-Locked Loop) that achieves fast locking and low Power consumption using a new locking algorithm. A fashion for a fast locking speed is that controls the two controller in sequence. The up/down signal due to clock skew between a internal and a external clock in phase detector, first adjusts a large phase difference in coarse controller and then adjusts a small phase difference in fine controller. A way for a low power consumption is that only operates one controller at once. Moreover the proposed DLL shows better jitter performance Because using the lock indicator circuit. The proposed DLL circuit is operated from 50MHz to 200MHz by SPICE simulation. The estimated power dissipation is 15mA at 200MHz in 3.3V operation. The locking time is within 7 cycle at all of operating frequency.

  • PDF