• 제목/요약/키워드: dual culture

검색결과 264건 처리시간 0.026초

도박이용자의 기본심리욕구 만족이 도박행동에 미치는 영향: 일반 동기와 대인존재감의 이중매개효과 (The Effect of the Gambler's Basic Psychological Needs Satisfaction on Gambling Behavior: The Dual Mediating Effects of General Motivation and Mattering)

  • 김서희;신성만
    • 한국심리학회지 : 문화 및 사회문제
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    • 제27권4호
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    • pp.585-607
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    • 2021
  • 본 연구는 자기결정성 이론을 기반으로 도박행동에 영향을 미치는 변인을 검증하고 도박행동에 대한 개입방안을 모색하기 위해 기본심리욕구 만족이 일반 동기 수준과 대인존재감을 통해 도박행동 심각도와 저수준 도박행동에 미치는 영향을 각각 살펴보았다. 이를 위해, 최근 3개월간 1회 이상 도박에 참여한 성인 402명의 설문 응답 자료를 사용하여 다중매개모형 분석을 실시하였다. 분석 결과, 기본심리욕구의 만족이 도박행동 심각도에 미치는 영향에서 일반 동기수준과 대인존재감의 이중매개효과가 유의한 것으로 나타났다. 구체적으로, 기본심리욕구의 만족은 일반 동기 수준을 매개로 하여 대인존재감에 유의한 정적 영향을 미쳤으며, 이러한 경로를 통한 대인존재감은 도박행동 심각도에 유의한 부적 영향을 미치는 것으로 나타났다. 반면, 기본심리욕구의 만족은 저수준 도박행동에 유의한 부적 영향을 미쳤으나 일반 동기 수준과 대인존재감의 이중매개효과는 유의하지 않은 것으로 나타났다. 연구결과를 통해 도박이용자의 일상에서 나타나는 동기와 대인존재감이 도박행동에 미치는 영향에 대하여 논의하였으며, 제한점과 후속 연구를 위한 방향성을 제언하였다.

양어수경 복합시설에 수로형 사육시설의 적용 (Application of Channel Type Aquaculture System to the Complex Facilities with Tilapia Rearing and Hydroponics)

  • 이병일;이준구;홍상근;홍석우;배용수;김기덕
    • 생물환경조절학회지
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    • 제8권3호
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    • pp.209-215
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    • 1999
  • 수로형의 양어시설에서 틸라피아 사육시 성장도 및 사육수의 수질을 파악하여 수경재배시설에 수로형 사육시설의 병용 가능성을 조사하고, 펜타이트 파이프를 이용한 간이 수로형사육시설을 개발하고자 시험을 수행하였다. 폭 2m, 높이 1m,길이 19m의 수로형 사육시설 위에 수경 베드를 설치하고 틸라피아를 양식하였다. 수질은 사육하는데 이상 없이 유지되었고, 수로형 시설에서의 틸라피아의 성장은 원형사육시설에서의 그 것과 대등하였다. 따라서 수경재배시설에 수용할 수 있는 양어시설은 수로형이 적합하며 사육환경이 정상적이므로 수로형 사육시설의 도입이 가능하다고 판단되었다. 또한 양어수경 복합영농어시설은 높이 1 m, 폭 1.4 m, 길이 20 m의 크기로, 기둥은 25mm의 펜타이트 파이프와 사육조는 5m의 PP판넬을 사용하도록 하였으며, biofilter로는 모래를 10cm 정도 채워 작물을 재배하도록 하는 간이수로형 양어수경 복합시설의 구조를 고안하였다.

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Isolation and Numerical Identification of Streptomyces humidus strain S5-55 Antagonistic to Plant Pathogenic Fungi

  • Lim, Song-Won;Kim, Jeong-Dong;Kim, Biom-Seok;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • 제16권4호
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    • pp.189-199
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    • 2000
  • To search for the antifungal substances, various actino-mycete isolates were obtained from various soils of Korea using plate dilution method on the humic acid vitamin agar plates. In the screening procedures using a dual culture method, 32 actionomycete isolates were selected, which showed the inhibitory activity against mycelial growth of plant pathogenic fungi Altirnaria mali, Colletotrichum gloeosporides, Fusarium oxysporum f.sp. cucumerinum, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. Bioassay of the crude extracts from culture filtrates and mycelial mets revealed that 12 antagonistic actionomycetes produced highly active antifungal substances. Actinomycete strain S5-55 which showed the substantial antifungal activity against the tested fungi was selected for production of the antifungal substances. Based on the cytochemical and morphological characteristics, strain S5-55 was identified as a Streptomyces species. The results of the numerical identification using the TAXON program confirmed that Streptomyces strain S5-55 was identical with Streptomyces humidus including in TAXON major cluster 19. The production of antifungal substance was most favorable when S. humidus strain S5-55 was cultivated for 10 dats on soluble starch broth supplemented with $K_2$HPO$_4$. The antifungal substances active against the plant pathogenic fungi P. capsici and M. grisea were partially purified using $\textrm{C}_{18}$ reversed-phase column chromatography.

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Design of Closed Seawater Recirculating Aquaculture System for Korean Rockfish Sebastes schlegeli Culture

  • Peng, Lei;Oh, Sung-Yong;Jo, Jae-Yoon
    • Ocean and Polar Research
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    • 제26권1호
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    • pp.102-111
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    • 2004
  • Recirculating aquaculture system (RAS) consists of different treatment compartments that maintain water quality within the ranges commonly recommended for fish cultures. However, common RASs still exert considerable environmental impact since concentrations of organic matter and nutrients in their effluents are high. Compared with the traditional RAS, the model RAS developed here use a sedimentation basin for digestion purposes and then use the released volatile organic matter to stimulate a denitrification process. Different treatment compartments for solids, total ammonia nitrogen, and nitrate removal have been reviewed. This paper provides the basic information on designing different treatment compartments as well as the engineering criteria in closed seawater RAS, consisting of circular tanks for fish cultures; dual drain systems, sedimentation basins and foam fractionators for removal of solids; nitrification biofilters for TAN removal; denitrification biofilters for nitrate removal; and aerators for aeration. The main purpose is to outline a common procedure in designing of closed RAS for marine fish culture with an emphasis on easy management and low expense, as well as reduction of the environmental impact.

아르떼 포베라(Arte Povera)가 현대패션에 미친 조형적 특징에 관한 연구 (A Study on the normativeness of Modern Fashion through Arte Povera)

  • 엄소희
    • 복식문화연구
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    • 제10권3호
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    • pp.270-282
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    • 2002
  • This study was established to understand how Arte Povera has affected the contemporary fashion based on the analyses of its characteristics and may provide a prospective view to predict style of future fashions. The results were as follows : 1) “The poverty” shows such characteristics as patch work, intentional mismatch, and tendency to prefer slightly used style. This meaning can be explained as direct expression from a sense of alienation, against to consumer economy, and dual-expression between poverty and wealth. 2) “Anti-formativeness” can be described for misinterpretations of creation, arrangement, and informal silhouette due to distortion of the human body. This expression includes such meanings as against to traditionalism, ignorance of rationality, and disorganization of the concepts between the beauty and the ugly. 3) “Naturalism” indicates as characteristics of use of natural materials and primitive expression. This expression could be generated from adaptation to the nature and desire toward uncivilized society due to skepticism about modern society. 4) “Symbolism” shows such expressions as an isolated feeling from society, and metaphoric sense from specific situations because there are too many applications of imported materials. This tendency can be also explained with the creations of spaces for opened concepts through disorganizations of territory, obscurity, and irregularity.

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Growth Promoting Rhizospheric and Endophytic Bacteria from Curcuma longa L. as Biocontrol Agents against Rhizome Rot and Leaf Blight Diseases

  • Vinayarani, G.;Prakash, H.S.
    • The Plant Pathology Journal
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    • 제34권3호
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    • pp.218-235
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    • 2018
  • Plant growth promoting rhizobacteria and endophytic bacteria were isolated from different varieties of turmeric (Curcuma longa L.) from South India. Totally 50 strains representing, 30 PGPR and 20 endophytic bacteria were identified based on biochemical assays and 16S rDNA sequence analysis. The isolates were screened for antagonistic activity against Pythium aphanidermatum (Edson) Fitzp., and Rhizoctonia solani Kuhn., causing rhizome rot and leaf blight diseases in turmeric, by dual culture and liquid culture assays. Results revealed that only five isolates of PGPR and four endophytic bacteria showed more than 70% suppression of test pathogens in both assays. The SEM studies of interaction zone showed significant ultrastructural changes of the hyphae like shriveling, breakage and desication of the pathogens by PGPR B. cereus (RBacDOB-S24) and endophyte P. aeruginosa (BacDOB-E19). Selected isolates showed multiple Plant growth promoting traits. The rhizome bacterization followed by soil application of B. cereus (RBacDOB-S24) showed lowest Percent Disease Incidence (PDI) of rhizome rot and leaf blight, 16.4% and 15.5% respectively. Similarly, P. aeruginosa (BacDOB-E19) recorded PDI of rhizome rot (17.5%) and leaf blight (17.7%). The treatment of these promising isolates exhibited significant increase in plant height and fresh rhizome yield/plant in comparison with untreated control under greenhouse condition. Thereby, these isolates can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.

잔디 Large patch의 생물학적 방제를 위한 길항 미생물의 선발과 항균력 검정 (Antimicrobial test of Antagonistic Microbes for Biological Control of Large patch of Zoysiagrass)

  • 마기윤;이긍주
    • 한국잔디학회:학술대회논문집
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    • 한국잔디학회 2011년도 제24차 정기총회 및 학술발표회
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    • pp.35-35
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    • 2011
  • A large patch disease caused by Rhizoctonia solani AG2-2(IV) is a serious problem in turfgrass sites including golf courses and sports fields in Korea. The objectives of this study were to isolate some antagonistic microorganisms and to explain some involving mechanisms. Initially single colonies which were formed from the filtrates of various soil samples were obtained from LB culture and then co-cultured with R.solani AG2-2(IV) on PDA plate to explore some antagonistic microbes against for large patch fungus, Rhizoctonia solani AG2-2(IV). Out of total 82 antagonistic isolates which commonly had inhibition effect on Rhizoctonia solani AG2-2(IV) mycelial growth, one candidate (YPIN22) showed the most antifungal effect, which was confirmed by the longest distance from the edge of bacterial colony to the mycelial edge of the Rhizoctonia solani AG2-2(IV) in the dual culture. A succeeding investigation was to test any potential effect of the isolate on growth inhibition of 5 other turfgrass pathogens including R. solani solani AG2-2(IIIB), P. ultimum, C. caudatum, C. lunata, and F.oxysporum. Preliminary result indicated that the new isolate YPIN22 was also found to have antagonistic potential on the growth inhibition of those turfgrass pathogenic fungi, which was explained by inhibition zones ranging from 8 to 22mm. A further explanation of some characteristics of the isolate YPIN22 will be discussed in detail.

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Antagonism and Structural Identification of Antifungal Compound from Chaetomium cochliodes against Phytopathogenic Fungi

  • Kang, Jae Gon;Kim, Keun Ki;Kang, Kyu Young
    • Journal of Applied Biological Chemistry
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    • 제42권3호
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    • pp.146-150
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    • 1999
  • As a part of the integrated disease system in greenhouse, an antifungal fungus(AF1) was isolated from greenhouse soil. It exhibited strong inhibitory activites against Pythium ultimum, Phytophtora capsici, Rhizoctonia solani, Botrytis cinerea, and Fusarium oxysporum based on dual culture on 1/5 strength of potato dextrose agar between antagonistic fungus and several plant pathogens. The antagonistic fungus was identified as Chaetomium cochliodes, based on morphological characteristics; the body of the perithecium bears straight or slightly wavy, unbranched hairs, whilst the apex bears a group of spirally coiled hairs. To investigate antagonistic principles, antifungal compound was extracted and fractionated by different solvent systems. An antifungal compound was isolated as pure crystal from is culture filtrate using organic solvent extraction and column chromatography, followed by preparative thin layer chromatography. The chemical structure of the purified antifungal compound was identified as chaetoglobosin A based on the data obtained form $^1H-NMR$, $^{13}C-NMR$, DEPT 90, 135, $^1H-^1H$ COSY, $^1H-^{13}C$ COSY and EI/MS. $ED_{50}$ values of the chaetoglobosin A against P. ultimum, P. capsici, R. solani, B. cinerea and F. oxysporum were 1.98, 4.01, 4.16, 2.67 and 35.14 ppm, respectively.

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Effects of Interleukin-$1\beta$, Tumor Necrosis Factor-$\alpha$ and Interferon-$\gamma$ on the Nitric Oxide Production and Osteoclast Generation in the Culture of Mouse Bone Marrow Cells

  • Kwon, Young-Man;Kim, Se-Won;Ko, Seon-Yle
    • International Journal of Oral Biology
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    • 제31권2호
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    • pp.67-72
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    • 2006
  • Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of $30{\mu}M$ SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration($3{\mu}M$) of SNP enhanced it. Although the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone metabolism and bone pathophysiology.

Phylogenetic Diversity and Antifungal Activity of Endophytic Fungi Associated with Tephrosia purpurea

  • Luo, Ze-Ping;Lin, Hai-Yan;Ding, Wen-Bing;He, Hua-Liang;Li, You-Zhi
    • Mycobiology
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    • 제43권4호
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    • pp.435-443
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    • 2015
  • Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.