• The Korean Journal of Ecology
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• v.26 no.1
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• pp.5-12
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• 2003

Seed germination rate and seedling growth were measured on 6 different species(Phytolacca americana, Eupatorium rugosum, Rumex acetocella, Echinochloa crusgalli, Cassia mimosoides var. nomame, Setaria viridis) treated with leaf extract of E. rugosum. Total phenolic compound and heavy metal were analyzed on leaf and soil with and without E. rugosum. The growth of P. americana seedlings were stimulated by 10% and 25% of E. rugosum water extract treatment. The content of total phenolic compounds in soil was lower than that of leaf extract, and 25% was confirmed as threshold concentration in natural systems because the total phenolic compounds were not significantly different between the control soils and the soil treated with 10%, and 25% extract. Total phenolic compound concentrations of the leaf extracts were highest (1.66 mg/l) with E. rugosum grown under the Quercus forest canopy and lowest (1.09 mg/l) for the plant grown in the mixed forest edge. Leaf extracts of plants selected in different sampling sites (Forest interior, Forest edge, under Pinus Canopy and Quercus Canopy) were significant, while soil extracts were not. Seed germination of R. acetocella and S. viridis were significantly inhibited at over 50% concentrations of E. rugosum, but C. mimosoides var. nomame was not affected at any concentration. The radicle and shoot growth of the native species group were reduced two times more than those of the exotic species group by the treatment of extracts. Especially, the seed germination percentage and dry weight of E. rugosum were greater than those of the control group by treatments with extracts of 10% and 25%. Analysis of aqueous extracts from E. rugosum by HPLC identified 6 phenolic compounds: caffeic acid (460.9 mg/l), benzoic acid (109.7 mg/l), protocatechuic acid (7.3 mg/l), ρ-hydroquinone (6.0 mg/l), cinnamic acid (2.7 mg/l) and hydroquinone (0.23 mg/l). The seed germination of P. americana was also inhibited dramatically by protocatechuic acid and cinnamic acid even though the content of caffeic acid (460.9 mg/l) was the highest among analyzed phenolic compounds. The heavy metal content of soil without A. altissima was higher than that of soil with E. rugosum. Particularly, Al, Fe and Mn was considerably high and most of the heavy metal were accumulated in leaves where a high level of total phenolic compounds was found.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.2
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• pp.112-129
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• 2012

The aim of this study was to provide basic data in managing the project that was carried out on Bulgwangcheon in a nature-friendly way to improve the conditions around the areas, which was brought to completion in 2002, based on changes in ecological characteristics. For this propose, this study examined documents related to the project, compared physical and enviromnental changes before and after the project was conducted and analyzed changes in the stream ecosystem. The result showed that in areas that effluent water was often observed, especially when it rained, the river wall was washed away and vegetation was found damaged. As for actual vegetation, this study compared planting coverage of each section of the research area and actual vegetation charts. The results indicated that Lespedeza spp., Aster koraiensis among mixed seeds that were planted in the reservoir path were almost swept away while Festuca arundinacea dominated the areas. Phragmites communis, Miscanthus sacchariflorns and Salix gracilistyla which had been planted in a small number were also almost washed out though a small number of them were left to form a colony. After examining the topography and structure of the plant community, this study found that areas where mixed seed were planted had changed into two types of vegetation: First type of area is dominated by P and R which are usually raised in apron with abundant floating particles. The second type of area is dominated by dry gramineous plant such as F and A. Areas around low flow channel where Phragmites communis, Miscanthus sacchariflorus and Salix gracilistyla planting construction method is applied was washed away with the width of low flow channel reduced. Though P, M and S formed a small community in some areas around the low flow area, they were in small number and in composition of simple plant species. Two ways were suggested in this study to manage the stream in an ecological way. First, adequate revetment construction methods should be applied by monitoring the flow of the stream as well as considering the flood control of urban streams. Second, target vegetation communities that are suitable for the environment of the stream should be chosen and be plantedconstantly with high density. At the same time, ornamental native plants shouldn't be planted as they have been and disturbing vegetation should be removed.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.4
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• pp.333-340
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• 2010

Wastewater treatment using algal communities and biodiesel production from wastewater-cultivated algal biomass is a promising green growth technology. In literature, there are many studies providing information on algal species producing high content of lipid. However, very little is known about adaptability and wastewater treatability of such high-lipid algal species. In this study, we attempted to characterize algal biomass production and wastewater treatability of high-lipid algal species under municipal wastewater condition. For this, four known high-lipid algal strains including Chlorella vulgaris AG 10032, Ankistrodesmus gracilis SAG 278-2, Scenedesmus quadricauda, and Botryococcus braunii UTEX 572 were individually inoculated into municipal wastewater where its indigenuous algal populations were removed prior to the inoculation, and the algae-inoculated wastewater was incubated in the presence of light source (80${\mu}E$) for 9 days in laboratory batch reactors. During the incubations, algal biomass production (dry weight) and the removals of dissolved organics (COD), nitrogen and phosphorous were measured in laboratory batch reactors. According to algal growth results, C. vulgaris, A. gracilis and S. quadricauda exhibited faster growth than indigenuous wastewater algal populations while B. braunii did not. The wastewater-growing strains exhibited efficient removals of total-N, ${NH_4}^+$-N, Total-P and ${PO_4}^{3-}$-P which satisfy the Korea water quality standards for effluent from municipal wastewater treatment plants. A. gracilis and S. quadricauda exhibited efficient and stable treatability of COD but C. vulgaris showed unstable treatability. Taken together with the results, A. gracilis and S. quadricauda were found to be suitable species for biomass production and wastewater treatment under municipal wastewater condition.

• Korean Journal of Environmental Agriculture
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• v.23 no.4
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• pp.211-219
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• 2004

Three sludge types from pharmaceutical byproducts and one sludge type from cosmetic waste-water sludge as raw materials of compost were used in a field based concrete pot ($4\;m^2$, $2\;m{\times}2\;m$) for investigating damage of red pepper cultivation. These sludges and pig manure (1 Mg/10a, dry basis) were incorporated into the upper of clay loam soil prior to transplanting with red pepper. Changes in concentration and properties of heavy metal for both of soil and plant were investigated 4 times during of red pepper growth. Plant height and stem diameter of red pepper in sludge treatments except to Pharmaceutical sludge 3 were poor than those of NPK treatment. This result were regarded as an effect of incompleted decomposition sludge which has a lot of organic matter concentration. Amount of total As was increased rapidly Jul. 8. in soil, total Zn Cu Pb Cd were in harvest time, and 1 N-HCl extractable Zn Cu Pb Cd As were in harvest at middle stage and then decreased. Amounts of nitrogen in plant (leaf and stem) were high in Phamaceutical Sludge 1 and fig Manure treatment in early and middle stage because of organic matter and nitrogen concentrations and characteristics. Amounts of Zn, Pb, and Ni in leaf and amount of Zn and Pb in stem were increased in harvest time so that we need to have a concern in detail. Total yield of red pepper was Pig Manure > Phamaceutical Sludge 3 > Phamaceutical Sludge 1 > NPK > Phamaceutical Sludge 2 and Cosmetic Sludge treatment was decreased considerably to compare to others. Amounts of Zn and Cu in green and red pepper in harvest time were higher than the other heavy metals. Finally these results can use to utilize that finding damage on crop for authorization and suitability estimation of raw material of compost.

• Korean Journal of Agricultural and Forest Meteorology
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• v.2 no.4
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• pp.183-189
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• 2000

This study was carried out to analyze ecophysiological responses for seedling of Quercus serrata, Quercus mongolica and Quercus variabilis that are the typical species of deciduous broadleaved forests in Korea. And executed experiments in the climatic control room to provide necessary information to ecological forest management and methods of natural regeneration through the analysis of seedling responses. The details of experimental analysis were growth processes of 4 months after seeding that vary with the condition of three light intensity[relative light intensity(RLI) 8%, 20%, 52%] and three soil moisture[water suction(WS) Ψ=100 hPa, Ψ=280 hPa, Ψ=330 hPa] gradient, growth factors after harvesting and the nutrition condition of leaves. The results of this study are followings: 1) Early growth was prosperous after germination for the species which have more weight of acorn. 2) The formation of lammas shoot was favourable with Q. variabilis and Q. mongolica. And the rate of the occurrence was the highest in the RLI 20%, and it was remarkably reduced in the RLI 8%. 3) As the height growth of seedling of all 3 species were greater in the RLI 20% and 8% than that of the RLI 52%, they showed strong shade tolerance. 4) The increase of light intensity promoted the diameter at root collar growth, and development of main and lateral roots with all 3 species. 5) It showed that the increase of light intensity in the experimental radiation condition raised special leaf area weight(mg/cm$^2$) and leaf area productivity(mg/cm$^2$). Consequently, these resulted in the increase of leaf thickness and total dry biomass per the unit area of leaf. 6) As the increase of light intensity, the minerals contents of leaves such as N, P and K were lowered, and the increase of soil moisture resulted in the increase of P, K, Ca and Mg.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.323-332
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• 2003

This study was carried out to provide information for the present status of soil pollution near abandoned old-zinc mining area through analysis of bound form and 0.1 N-HCl extractable concentrations of heavy metals in soils and plants. Feasibility of endemic plants for phytoremediation was evaluated by the investigation of vegetation in soils. Cd contents of the selected samples near old-zinc mining soils ranged from 0.2 to $42mg\;kg^{-1}$. Nonagricultural soils near the mining area contained great amounts of Zn, Pb, Cd, and Cu than the paddy and upland soils. Some Korean wild plants, Artemisia princeps, Artemisia montana, Erigeron canadensis, and Pueraria thunbergiana, were found to grow vigorously in the studied area. Among them, Artemisia princeps was selected as a possible phytoremediator for cleaning heavy metal contaminated soils. Artemisia princeps contained about 43 and $52mg\;kg^{-1}$ of Cd in their root and shoot as dry weight, respectively. Average contents of Cd in the rhizosphere soil, $15.68mg\;kg^{-1}$, was slightly higher than the soil-root interface soils, $14.1mg\;kg^{-1}$. Sequential extraction of Cd contaminated soils showed that average $2.4mg\;kg^{-1}$ (about 7%) of cadmium existed as exchangeable form and the average amounts increased as follows : adsorbed < organically bound < exchangeable << oxide carbonate << sulfide residual fractions. Amendment of organic by-product fertilizer in metal-contaminated soils promoted the growth of roots significantly as compared with the other treatments containing chemical fertilizer.

• The Korean Journal of Mycology
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• v.10 no.1
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• pp.33-39
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• 1982

Carpophores of ten Korean strains of Lentinus edodes (Berk.) Singer, an antitumor polysaccharide producing fungus, were extracted with 0.1N NaOH solution. The extracts were dialized for seven days in distilled water and lyophilized to produce crude polysaccharide powders. Thus obtained crude polysaccharide samples were assayed for sugar contents by colorimetric method with anthrone reagent. Among ten strains examined Lentinus edododes-DMC7 was found to be the richest strain in polysaccharide content of carpophores. By shake culture experiment for biomass production, L. edodes-DMC7 was found to be the second most productive strain among seven strains examined. Cultural characteristics of L. edodes-DMC7 were investigated by shake culture method. The best result was obtained when L. edodes-DMC7 was cultured in the medium containing glucose 8g, starch 80g, yeast extract 12g, $KH_2PO_4\;0.87g,\;MgSO_4{\cdot}7H_2O\;O.5g,\;CaCl_2\;0.3g,\;FeSO_4{\cdot}7H_2O\;10mg\;ZnSO_4{\cdot}7H_2O\;4mg,\;CuSO_4{\cdot}5H_2O\;lmg,\;MnCl_2{\cdot}4H_2O\;7mg\;per\;11\;at\;28^{\circ}C$, 180 rpm, for 12 days. Thus thirty-three grams of dry mycelia was obtained per one liter of medium.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.2
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• pp.108-117
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• 2005

Pharmaceutical byproduct sludge and cosmetic industry wastewater sludge can be used as a raw material of compost. Effects of three types of pharmaceutical byproduct sludge and one type of cosmetic industry wastewater sludge on soil properties and red pepper growth were investigated in a field based concrete pot ($2{\times}2m$). These sludges and pig manure ($5Mg\;ha^{-1}$, dry basis) were incorporated into the upper of loam soil 30 days prior to transplanting red pepper. Changes in soil properties and contents of heavy metals and toxic organic compounds in soil and plant were measured. And also plant growth measurement and bioassay of soil phytotoxicity were included. Contents of heavy metals were increased in the soils treated with the sludges. Plant growth in the sludge treatments were mostly inferior to that of NPK treatment, especially in early stage. Content of N in plant was lower in all sludge treatments at early and middle growth stages, and it was especially caused by characteristics and concentration of nitrogen and organic matter of sludges. Total yield of red pepper was highest in the NPK treatment and followed by pharmaceutical sludge 3, pig manure, pharmaceutical sludge 1, and pharmaceutical sludge 2, and the yield of cosmetic sludge treatment was considerably lower than others. HEM and PAHs contents in soil of cosmetic sludge treatment were $4.80mg\;kg^{-1}$ and $2,263.2{\mu}g\;kg^{-1}$, respectively. Root elongation of lettuce exposed to the water extract of soil treated with cosmetic sludge was about 20% of that found in the test with soil extract of non fertilization treatment. At present, raw materials of compost were authorized according to the contents of organic matter, heavy metals and product processing. Toxic organic compounds analysis and bioassay would be helpful for authorization and assessment of suitability of raw materials of compost.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.1-14
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• 1986

This study was carried out to acguire some basic information on nutritional and lhysiological effects of vesicular arbuscular mycorrhizae(VAM) on mulberry trees inoculated with Glomus mosseae, Gerd. & Trap. grown in clay for 65 days and treated with 5 different levels of phosphorus, ie 30, 60, 120, 240, 480 ppm as (NH4)2 HPO4. At the End of the expermental period the levels of fixed phosphate in the soil was measared. And the native VAM fungi were collected to select the most effective VAM species on mulberry tree. The nutritional and biochemical effects of VA mycorrhizae on the mulberry leaves were also studied. Those results are as follow. 1. The mulberry trees grown in clay and inoculated with VAM were heavier in shoot dry weight as much as 197% than uninoculated plants. But in vermioulite, uninoculated mulberry trees were heavier as much as 135% than inoculated. 2. The rates of endo mycorrhizal formation in clay was highest at 60 ppm level of phosphorus, and vesicles in roots were formed in 240ppm and 480ppm level of phosphorus, but not in 30ppm, 60ppm and 120ppm. 3. The greatest growth responses of VAM inoculation was found at 60ppm level of hosphorus, and the optimum phosphorus level for VAM responese appeared to be 60ppm. 4. VAM was also to absorb soil-fixed phosphate. VAM abosrbed Fe-bound phosphate most efficiently and Ca-bound phosphate with ease but not Al-bound phosphate and Al-Fe occluded phosphate. 5. Three species of Gigaspora and one species of Glamus growing naturally in mulberry plantations were collected and tested for the growth responses. Gigaspora tricalipta and Gigaspora calospora revealed the greatest growth responses on mulberry tree among tested VAM fungi. 6. Mulberry leaves inoculated with VA mycorrhizal fungi contained 9.8% more phosphate and 15.2% more nitrogen, 22.2% more water-soluble carbohydrates and 15.2% more proteins than uninoculated plants. 7. The electrophoretic pattern of mulberry leaf protein inoculated with VAM fungi has 19 bands. 8. The patterns of peroxidase zymogram and Amulase zymogram were different between the mulberry leaves inoculated and uninoculated with VA mycorrhizal fungi. The peroxidase zymogram of inoculated leaves has 1 less majour band than unioculated leaves The amylase zymogram of inoculated leaves has 2 bands near the +pole, but that of uninoculated leaves has 1 band near the $pole.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.5
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• pp.364-370
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• 2009

This study was conducted to survey, analyze on the compaction layer and the plow layer at Jeonbug and Jisan series paddy soil, which is the representative soil in fluvio-marine and local alluvium, respectively. The depths of surface soil were 12.6 and 12.7 cm in Jeonbug and Jisan series, respectively. A plowing depth was 10.5 cm. The properties of compaction layer in two soil series were as follows. The hardness were $14.7kg\;cm^{-2}(25.3mm)$ and $8.7kg\;cm^{-2}(22.1mm)$ in Jeonbug and Jisan series, respectively. The thickness were 22.3 cm and 17.8 cm in Jeonbug and Jisan series, respectively. The depth of soil compaction, which means depth from surface, were 15 and 20 cm in Jeonbug and Jisan series, respectively. The relationship between the hardness of compaction layer and the depth of surface soil showed negative correlation, however relationship between the hardness and the thickness of compaction layer showed positive correlation. Soil temperature was lower in compaction layer than in plow layer. This temperature differences between compaction layer and plow layer were from 1.0 to $2.5^{\circ}C$ in Jeonbug series and from 0.7 to 2.1 in Jisan series. The soil physical properties of compaction layer were higher in bulk density and solid phase and lower in porosity and gaseous phase than those of plow layer in all soil series. The soil chemical properties of compaction layer were higher in pH, content of available silicate, exchangeable calcium and magnesium but lower in total nitrogen, content of organic matter and available phosphate than those of plow layer in all soil series. Cation exchangeable capacity and content of exchangeable potassium were similar between compaction layer and plow layer in Jeonbug series, however, in Jisan series these were lower in compaction layer than in plow layer. Elution amount of inorganic nitrogen were lower in compaction layer than in plow layer in all soil series. The content of soluble Fe and Mn were plenty in compaction layer compared with plow layer and these tendency was apparent in Jeonbug series. The water depth decrease were fast until the latter part of June, and were slow as $1{\sim}3mm\;day^{-1}$ for July and August, and were fast again from september. Rice roots distributions as each soil series and tillage method were 25 cm at rotary plowing in Jeonbug series, 30 cm at deep plowing in Jeonbug series, and 20 cm at tillage in Jisan series. Dry weight per m2 at heading stage were much in order of deep plowing in Jeonbug series, rotary plowing in Jeonbug series, and tillage in Jisan series.